• 한국식품영양과학회지
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• 제45권2호
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• pp.293-297
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• 2016

HMG-CoA reductase는 체내 콜레스테롤 생합성에 있어서 속도제한단계(율속) 효소이다. 본 연구는 HMG-CoA reductase에 대한 이소플라본 배당체의 저해 효과를 연구하였다. $100{\mu}M$의 농도에서 genistein-7-O-triglucoside(G2-genistin)는 HMG-CoA reductase 활성을 약 18% 정도 저해하였으나, daidzein-7-O-triglucoside는 저해 효과를 갖지 않았다. 시리아햄스터 HMG-CoA reductase의 반응속도 실험에서 G2-genistin은 농도에 관계없이 $V_{max}$의 저해 정도가 일정하였으며, 이것은 G2-genistin이 HMG-CoA reductase의 경쟁적 저해제로 작용함을 시사하고 HMG-CoA reductase의 활성을 직접적으로 저해함으로써 혈중 콜레스테롤 함량을 감소시킬 수 있음을 예상할 수 있다.

• Journal of Microbiology and Biotechnology
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• 제23권4호
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• pp.451-458
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• 2013

The human gastric pathogen Helicobacter pylori (Hp) has been considered a microaerophile. However, we recently reported that, when supplied with 10% $CO_2$, Hp growth is stimulated by an atmospheric level of $O_2$, suggesting that Hp is a capnophilic aerobe. In this study, we investigated the effects of aerobic $O_2$ tension on Hp cells by comparing gene expression profiles of cultures grown under microaerobic and aerobic conditions in the presence of 10% $CO_2$. The results showed that overall differences in gene expression in Hp cells grown under the two $O_2$ conditions were predominantly growth-phase-dependent. At 6 h, numerous genes were down-regulated under the aerobic condition, accounting for our previous observation that Hp growth was retarded under this condition. At 36 h, however, diverse groups of genes involved in energy metabolism, cellular processes, transport, and cell envelope synthesis were highly up- or down-regulated under the aerobic condition, indicating a progression of the cultures from the log phase to the stationary phase. The expression of several oxidative stress-associated genes including tagD, katA, and rocF was induced in response to aerobic $O_2$ level, whereas trxA, trxB, and ahpC remained unchanged. Altogether, these data demonstrate that aerobic $O_2$ tension is not detrimental to Hp cells but stimulates Hp growth, supporting our previous finding that Hp may be an aerobic bacterium that requires a high $CO_2$ level for its growth.

• 한국기계가공학회지
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• 제18권3호
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• pp.14-25
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• 2019

From 2019 onwards, more stringent regulations (from Stage 4 to Stage 5) are to be implemented in Europe in order to reduce the air pollutant emissions. In South Korea, the government authorities started to make new regulation to meet the European regulation. As a first step, the air pollutant emissions such as CO, NOx, SOx, TSP, $PM_{10}$, $PM_{2.5}$, VOC, $NH_3$ by agricultural machinery were analyzed based on CAPSS inventory along with the analysis in the general aspect in this study. Three levels of analysis was conducted each in agricultural machinery aspect along with in the general aspect. Per agricultural tractor, all kinds of the air pollutant emissions decreased by 25, 25, 99, 25, 25, 25, 25% for the CO, NOx, SOx, TSP, $PM_{10}$, VOC, $NH_3$ emissions each from the year 2000 to the year 2014. Per combine harvester, all kinds of the air pollutant emissions decreased by 63, 63, 91, 63, 63, 63, 63% for the CO, NOx, SOx, TSP, $PM_{10}$, VOC, $NH_3$ emissions each from the year 2000 to the year 2014.

• 환경정책연구
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• 제12권2호
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• pp.59-86
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• 2013

1988년부터 2008년까지의 자료를 바탕으로 우리나라 6개 권역을 대상으로 권역별 소득, 환경규제, 오염유발원이 각 권역별 대기오염 물질(황산화물, 질소산화물, 총먼지, 일산화탄소)에 미치는 영향을 지역적 관점에서 살펴보았다. 즉, 환경 쿠즈네츠 곡선(EKC)가설 검증차원에서 공해배출과 경제성장 간의 관계를 패널회귀분석을 통해 분석하였다. 분석 결과, 산업시설에 영향을 미치고 있는 황산화물(SOx)의 경우 대부분의 권역에서 EKC가설이 성립하는 것으로 나타났지만, 질소산화물(NOx)과 총먼지(TSP)의 경우 6개 권역 중 대경권에서만 EKC가설이 성립하는 것으로 나타났 으며, 일산화탄소(CO)의 경우에도 EKC가설을 충족시키는 권역은 중부권, 호남권, 동남권에 한정되어 있었다. 또한 각 권역별 대기오염 단속규제가 대기오염과 정(+)의 관계를 보임에 따라 환경규제효과가 나타난다고 보기 어려우며, 각 권역별 연료소비와 석유화학제품의 특화 또한 권역별 대기오염을 증가시키는 주요 요인으로 작용하고 있다.

• 한국분무공학회지
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• 제22권2호
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• pp.47-54
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• 2017

$CO_2$ emission regulation will be prescribed and main issue in automotive industry. Mostly, vehicle's fuel efficiency deeply related to $CO_2$ emission is regulated by qualified driving test cycle by using chassis dynamometer and exhaust gas analyser. But, real driving fuel consumption rate depends so much on the individual usage profile and where it is being driven: city traffic, road conditions. In this study, vehicle model of fuel consumption rate for ICEV and PHEV was developed through co-simulation with CRUISE model and Simulink based on driving control model. The simulation results of fuel consumption rate were analysed with on-road vehicle data and compared with its official level.

• 해양환경안전학회지
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• 제21권2호
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• pp.147-153
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• 2015

국제해사기구는 선박기인 $CO_2$ 저감을 위해 SEEMP를 시행 하였다. 본 논문에서는 국제해사기구가 권고한 SEEMP 시행 방안과 세 해운선사의 SEEMP 적용 사례를 동일 기준으로 비교 분석 하였다. 이를 통해 적용상의 문제점을 파악하여 해운선사들이 SEEMP를 더욱 효율적으로 적용하기 위한 개선 방안을 제시하였다. 개선방안으로는 Company EEOI의 오류 수정, EEOI 데이터 획득 기간 통일, 에너지 효율 운항을 위한 조치사항 준수, 다양한 연료에 대한 $CO_2$ 환산 계수 제시, 국제해사기구의 공식적 기준을 통한 EEOI 목표값 설정이 있다.

• Molecules and Cells
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• 제40권5호
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• pp.322-330
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• 2017

This study investigated the role of cancer/testis antigen DDX53 in regulating cancer stem cell-like properties. DDX53 shows co-expression with CD133, a marker for cancer stem cells. DDX53 directly regulates the SOX-2 expression in anti-cancer drug-resistant $Malme3M^R$ cells. DDX53 and miR-200b were found to be involved in the regulation of tumor spheroid forming potential of Malme3M and $Malme3M^R$ cells. Furthermore, the self-renewal activity and the tumorigenic potential of $Malme3M^R$-CD133 (+) cells were also regulated by DDX53. A miR-200b inhibitor induced the direct regulation of SOX-2 by DDX53 We therefore, conclude that DDX53 may serve as an immunotherapeutic target for regulating cancer stem-like properties of melanomas.

• IMMUNE NETWORK
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• 제24권1호
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• pp.2.1-2.24
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• 2024

Studies over the last 2 decades have identified IL-17 and IL-21 as key cytokines in the modulation of a wide range of immune responses. IL-17 serves as a critical defender against bacterial and fungal pathogens, while maintaining symbiotic relationships with commensal microbiota. However, alterations in its levels can lead to chronic inflammation and autoimmunity. IL-21, on the other hand, bridges the adaptive and innate immune responses, and its imbalance is implicated in autoimmune diseases and cancer, highlighting its important role in both health and disease. Delving into the intricacies of these cytokines not only opens new avenues for understanding the immune system, but also promises innovative advances in the development of therapeutic strategies for numerous diseases. In this review, we will discuss an updated view of the immunobiology and therapeutic potential of IL-17 and IL-21.

• Preventive Nutrition and Food Science
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• 제8권1호
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• pp.61-65
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• 2003

This study was investigated to identify the regulatory mechanism of ACC gene expression by hormones and nutrition. The fragment of ACC promoter I (PI) -220 bp region was recombined to pGL3-Basic vector with luciferase as a reporter gene. The primary hepatocyte from the rat was used to investigate the regulation of ACC PI activity. ACC PI (-220 bp)/luciferase chimeric plasmid was transfected into primary rat hepatocyte by using lipofectin. ACC PI activity was shown by measuring luciferase activity. The addition of insulin, dexamethasone, and triiodothyronine to the culture medium increased the activity of ACC PI by 2.5-, 2.3- and 1.8-fold, respectively. In the presence of 1 $\mu$M dexamethasone, the effects of insulin was amplified about 1.2-fold showing the additional effects of dexamethasone. Moreover the activity of luciferase was increased by insulin, dexamethasone, and triiodothyronine treatment approximately 4-fold. These results indicated that insulin, dexamethasone and thyroid hormone coordinately regulate ACC gene expression via regulation of promoter I activity. On the -220 to ＋21 region of ACC PI, the addition of the glucose to the culture medium increased the activity of ACC PI. With 25 mM glucose, luciferase activity increased by 7-fold. On the other hand, on the -220 bp region, ACC PI activity was not changed by polyunsaturated fatty acids. Therefore, it can be postulated that there are response elements for insulin, triiodothyronine, dexamethasone, and glucose, but not PUFAs on the -220 bp region of ACC PI.

• Biomolecules & Therapeutics
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• 제19권2호
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• pp.211-217
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• 2011

Panax ginseng CA Meyer, a herb from the Araliaceae, has traditionally been used as a medicinal plant in Asian countries. Ginseng extract fermented by ginsenoside-${\beta}$-glucosidase treatment is enriched in ginsenosides such as Rh2 and Rg3. Here we show that a fermented ginseng extract, BST204, has anti-proliferative and anti-invasive effects on HT-29 human colon cancer cells. Treatment of HT-29 cells with BST204 induced cell cycle arrest at $G_1$ phase without progression to apoptosis. This cell cycle arrest was accompanied by up-regulation of tumor suppressor proteins, p53 and p21$^{WAF1/Cip1}$, down-regulation of the cyclin-dependent kinase/cyclins, Cdk2, cyclin E, and cyclin D1 involved in $G_1$ or $G_1/S$ transition, and decrease in the phosphorylated form of retinoblastoma protein. In addition, BST204 suppressed the migration of HT-29 cells induced by 12-O-tetradecanoylphorbol-13-acetate, which correlated with the inhibition of metalloproteinase-9 activity and extracellular signal-regulated kinase activity. The effects of BST204 on the proliferation and the invasiveness of HT-29 cells were similar to those of Rh2. Taken together, the results suggest that fermentation of ginseng extract with ginsenoside-${\beta}$-glucosidase enhanced the anti-proliferative and the anti-invasive activity against human colon cancer cells and these anti-tumor effects of BST204 might be mediated in part by enriched Rh2.