• 사상체질의학회지
• /
• 제17권1호
• /
• pp.130-141
• /
• 2005

1. Objectives The present study was carried out to investigate the effects of Chungpyesagan-tang on the $CCI_4-induced$ Liver Damage in Rats. 2. Methods Sprague-Dawley rats were devided into 5 experimental groups : Normal, $NS+CCI_4(Solid$ extract of $CCI_4$ injection group after Normal Saline feed), $CP+CCI_4(Solid$ extract of $CCI_4$ injection group after Chungpyesagantang feed), $CCI_4+NS(Nomal$ Saline feed group after $CCI_4$ injection), $CCI_4+CP(Solid$ extract of Chungpyesagantang feed group after $CCI_4$ injection). Biochemical assays for serum enzyme activities such as AST, ALT, ALP, BUN, Creatinine, Uric Acid, Total Protein, Albumin, Total Cholesterol, Triglyceride, Glucose, and mRNA Revelation of Cytochrome p450 and activities such as LPO(Lipid Peroxidation), GSH(Glutathione), GST(Glutathione-S- Transferase), Glutathione Reductase, Glutathione Peroxidase, SOD(Superoxide Dismutase), Catalase, Hydroxyproline, and ${\beta}-Glucuronidase$ were performed. 3. Results 1) $CP+CCI_4$ showed significantly lower relation of Cytochrome p450 than $NS+CCI_4$. 2) As to LPO Hydroxyproline, $CCI_4+CP$ showed significantly lower activity than $CCI_4+NS$. 3) As to GSH GST Glutathione Peroxidase Catalase, $CP+CCI_4$ showed higher activity than $NS+CCI_4$, $CCI_4+CP$ showed significantly higher activity than $CCI_4+NS$. 4) As to Glutathione Reductase SOD, $CCI_4+CP$ showed significantly higher activity than $CCI_4+NS$. 5) As to ${\beta}-Glucuronidase$, $CP+CCI_4$ showed signficantly lower activity than $NS+CCI_4$. 4. Conclusions Chungpyesagantang has the recovering effects on the $CCI_4-induced$ Liver Damage.

• Archives of Pharmacal Research
• /
• 제26권1호
• /
• pp.43-46
• /
• 2003

The anti-oxidant activities of tectochrysin, a major compound of propolis, were investigated. Tectochrysin exhibited a significant decrease in serum transaminase activities elevated by hepatic damage induced by $CCl_4$-intoxication in rats. Tectochrysin tested exhibited a lipid peroxidation causing a significant decrease in MDA production in TBA-reactant assay. Tectochrysin was strong in the increase in the anti-oxidant enzymes such as hepatic cytosolic superoxide dismutase, catalase and glutathione peroxidase activities in $CCl_4$-intoxicated rats. These results suggest that tectochrysin possess not only the anti-oxidant, but also the activities in $CCl_4$-intoxicated rats. Especially, tectochrysin was found to cause significant increases in the rat liver cytosolic SOD, catalase, GSH-px activities as well as a significant decrease in the MDA production.

• 대한약학회:학술대회논문집
• /
• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
• /
• pp.254.1-254.1
• /
• 2003

CCl4 has been known as typical chemical which induce acute hepatitis accompanying increase in the levels of acute phase proteins in serum. In this study, after acute liver damage was induced by CCl4 in Sprague-Dawley rats, the levels of serum acute phase proteins were examined using 2-dimensional electrophoresis and lactic dehydrogenase (LDH), alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels in blood were also examined at 1, 2, 3 days after the induction to confirm acute hepatitis. (omitted)

• 한국산학기술학회논문지
• /
• 제11권5호
• /
• pp.1936-1942
• /
• 2010

본 논문은 기능식품의 과용에 따른 간질환 환자에게 미치는 영향을 알아보기 위해, 마늘열수추출물의 용량을 달리하여, 사염화탄소에 의한 랫드의 간 손상을 유발하여 기능학적검사를 통해 간장에 미치는 용량의 중요성을 알아보았다. 실험동물은 Sprague-Dawley 종 웅성 랫드로 하여 정상군, 대조군, 양성대조군, 실험군 E1, E2, E3 군으로 나누어 각 군당 7마리씩 총 42마리를 이용하였고, 실험동물은 체중 당 사염화탄소 $0.5\;m{\ell}$ (0.20 g/kg/day)를 olive oil에 동량 희석하여 1일 1회 격일 간격으로 총 5회 복강 내 투여하여 간 손상 유발한 후 국내에서 유기농 재배한 마늘을 열수 추출하여 각 실험군에 체중 당 0.35 g/kg(E1), 0.70 g/kg(E2), 1.40 g/kg(E3)를 일일 1회, 주 5일, 4주간 위존데를 사용하여 투여하였다. 실험의 결과로 마늘추출물의 투여가 생리적 기능 활성에 긍정적인 작용을 하여 간장에서 산화스트레스를 감소시키고 독성 증감작용 및 기능학적 변화를 확인하였고, 용량에 따른 실험군(E1, E2, E3) 간의 비교에서 고농도 투여군(E3)에서는 마늘독성의 영향 때문에 E1, E2보다 긍정적인 효과를 덜 보였다.

• Biomolecules & Therapeutics
• /
• 제27권2호
• /
• pp.222-230
• /
• 2019

Intestinal barrier dysfunction always accompanies cirrhosis in patients with advanced liver disease and is an important contributor facilitating bacterial translocation (BT), which has been involved in the pathogenesis of cirrhosis and its complications. Several studies have demonstrated the protective effect of Vitamin D on intestinal barrier function. However, severe cholestasis leads to vitamin D depletion. This study was designed to test whether vitamin D therapy improves intestinal dysfunction in cirrhosis. Rats were subcutaneously injected with 50% sterile $CCl_4$ (a mixture of pure $CCl_4$ and olive oil, 0.3 mL/100 g) twice a week for 6 weeks. Next, $1,25(OH)_2D_3$ ($0.5{\mu}g/100g$) and the vehicle were administered simultaneously with $CCl_4$ to compare the extent of intestinal histologic damage, tight junction protein expression, intestinal barrier function, BT, intestinal proliferation, apoptosis, and enterocyte turnover. Intestinal heme oxygenase-1 (HO-1) expression and oxidative stress were also assessed. We found that vitamin D could maintain intestinal epithelial proliferation and turnover, inhibit intestinal epithelial apoptosis, alleviate structural damage, and prevent BT and intestinal barrier dysfunction. These were achieved partly through restoration of HO-1 and inhibition of oxidative stress. Taken together, our results suggest that vitamin D ameliorated intestinal epithelial turnover and improved the integrity and function of intestinal barrier in $CCl_4$-induced liver cirrhotic rats. HO-1 signaling activation was involved in these above beneficial effects.

• 한국식품저장유통학회지
• /
• 제23권2호
• /
• pp.275-282
• /
• 2016

본 연구는 4종의 별의별간(SS) 음료의 간보호 소재로서의 이용 가능성을 조사하고자 항산화능 평가, t-BHP 와 $CCl_4$로 산화적 손상 및 급성 간독성 유도한 in vitro, in vivo 모델을 활용하여 간보호능을 평가하였다. 실험결과, 별의별간 01~04는 $50{\mu}M$ vitamin C 와 유사한 항산화 효과를 나타내었다. HepG2 세포에 t-BHP로 산화 스트레스를 유도한 뒤 나타나는 세포독성에 대해 별의별간 01 및 04에서 농도 의존적인 세포 보호효과를 보였으며, ROS 생성 억제에서 별의별간 01, 03, 04에서 농도의존적인 억제를 나타내었다. 미나리가 혼합된 별의별간 04에 대한 급성 간손상 in vivo 모델을 활용하여 간보호능 검증 결과, 별의별간 04는 $CCl_4$로 증가된 혈중 ALT, AST의 유의적 감소, 간 조직중 증가된 MDA 함량 감소 및 감소된 GSH의 유의적 증가를 나타내었다. 또한, 혈청 및 간 조직에서 증가된 중성지방과 콜레스테롤을 유의적으로 감소시켰다. 이러한 결과를 종합하며, 별의별간 04는 in vitro 및 in vivo 모델에서 산화적 손상에 대해 간보호 효과를 나타내었다.

• 약학회지
• /
• 제48권1호
• /
• pp.34-40
• /
• 2004

Hovenia dulcis which is distributed in Korea, China and Japan is known to show hepatoprotctive effect and reduce the acute alcohol toxicity. In this study, the hepatoprotective effect against the chemically induced experimental liver injury models and lowering effect of blood alcohol level in animal models acutely administered alcohol by the peduncle extracts of Hovenia dulcis var. koreana were investigated. HdfHW-1, and HdfM-1 which are the extracts of fruit peduncles and young branches with hot water or 70％ methanol and followed with 100％ methanol, were significantly reduced the $CCl_4$ or D-galactosamine/LPS induced damage in sliced liver. The hot water or methanol extracts of fruit peduncle protected dose-dependently against $CCl_4$ induced toxicity in primary hepatocyte culture and particularly, the amount of LDH release was reduced to the control level at 500 $\mu\textrm{g}$/ml of hot water extracts. HdfHW-1 also decreased the hepatotoxicity induced by $CCl_4$ in rats. The active components of HdfHW-1 seemed to be high molecular weights because 0.2 M NaCl HdfHW-1 fraction was the most effective among NaCl fractions of HdfHW-1 eluted with various concentrations of NaCl on DEAE 650C column chromatography. HdfM and HdfHW were significantly reduced the levels of blood alcohol in rats and mice administered 40％ alcohol. These results indicated that the hot water or methanol extracts of fruit peduncle of Hovenia dulcis var. koreana have hepatoprotective effect and may be reduce alcohol toxicity.

• 대한약침학회지
• /
• 제8권3호
• /
• pp.31-38
• /
• 2005

Objectives : This study was aimed at investigating liver protection mechanism of bear bile juice (Fel Ursi)by inducing liver toxicity through $CCl_4$ in mice and evaluated histological and serological findings. Methods : Experiment groups was categorized into untreated normal group, $CCl_4$ treated control group, and orally administered bear bile juice experiment group. At the termination of experiment, gross examination of the liver as well as histological findings, and Total protein, Albumin, Total bilirubin, Direct bilirubin SGOT, SGPT, and ALP contents in the serum were evaluated. Results : 1. For gross examination and histological findings, $CCI_4$ treated control group showed destroyed lobular structure, increased fibrosis, as well as hepatic cirrhosis. For the group treated with bear bile juice, the lobular structure suffered less damage, and showed lower level of fibrosis and liver cirrhosis compared to the control group. 2. For serum analysis, Total protein and Albumin were significantly increased in the bear bile juice experiment group than the control group. Total bilirubin and Direct bilirubin didn't show significant differences between the two groups. SOOT, SGPT, and ALP were significantly decreased in the normal and bear bile juice experiment groups compared to the control group. Conclusion : Taken together, bear bile juice can be effectively used for recovering the liver functions and further researches must be conducted to verify the efficacies of bear bile juice.

• 동의생리병리학회지
• /
• 제22권1호
• /
• pp.155-161
• /
• 2008

Carbon tetrachloride $(CCl_4)-induced$ liver injury depends on a toxic agent that has to be metabolized by the liver NAPDH-cytochrome P450 enzyme system to a highly reactive intermediate. Although several isoforms of cytochrome P450 may metabolize $CC1_4$, attention has been focused largely on the cytochrome P450 2E1 (CYP2E1), which is ethanol-inducible. Alternations in the activity of CYP2E1 affect the susceptibility to hepatic injury from $CC1_4$. In this study, the liver protective effect of the hot water extracts of Scutellaria radix (SR) was investigated. The SR exhibited a hepatoprotective activity against $CCl_4-induced$ liver damage in Chang liver cells. The expression of CYP2E1, measured by RT-PCR and Western blot analysis, was significantly decreased by SR treatment in Chang cells. Based on these findings, it is suggested that hepatoprotective effect of SR possibly related to downregulation of CYP2E1 expression.

• 한국식품영양과학회지
• /
• 제33권8호
• /
• pp.1246-1251
• /
• 2004

본 실험은 사염화탄소 독성에 대한 몇 가지의 식물소재들- 솔잎, 콩나물, 인진쑥, 표고버섯 - 의 간보호 효과를 조사하기 위하여 수행되었다. 우선적으로 일차 간세포 배양실험에서 식물추출물(열수 또는 에탄올)을 첨가(20 mg/mL 배지)하고 2시간 후에 사염화탄소를 처리(50$\mu$L)한 결과, 배지내의 AST와 ALT 효소활성은 다음과 같았다. 솔잎은 에탄올 및 열수 추출물(P<0.05)에서, 표고버섯은 열수 추출물(p<0.01∼0.05)에서, 그리고 콩나물과 인진쑥은 추출방법에 따라 유의성 있는(p<0.01) 감소를 보였다. 또한, 3주간의 식물 추출물을 첨가한 사료를 사전급여하고 사염화탄소를 2일간 복강주사(1 mg/kg 체중)한 동물실험에서 혈청내의 AST 및 ALT 활성은 간세포 배양의 결과와 유사하게 솔잎과 콩나물에서 낮은 활성효과(p<0.01)를 보였다. 정상군에 대한 사염화탄소의 투여는 지질과산화물과 cytochrome P-450의 함량, 그리고 xanthine oxidase(XOD) 활성을 크게 증가시켰다. 그러나 식물소재 중에서 솔잎, 콩나물, 인진쑥 등을 추출물을 첨가한 실험군에 대한 사염화탄소 처리는 일반적으로 지질과 산화와 XOD 활성증가를 억제하는 것(p<0.01)으로 나타난 반면에, cytochrome P-450의 함량 증가는 솔잎과 인진쑥의 첨가군에서 관찰되었다. 따라서 본 실험에서 cytochrome P-430의 함량증가에 대한 식물 추출물들의 효과는 확실하지 않았지만, 솔잎과 콩나물의 추출물은 항산화관련 실험에서 대부분 탁월한 효과를 나타내었다.