• The Plant Pathology Journal
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• 제35권3호
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• pp.234-242
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• 2019

Thirty-four strains of bacteria were isolated from Phellodendron amurense. Using Nectria haematococca as an indicator strain, the best strain, B18, was obtained by the growth rate method. The morphological, physiological and biochemical characteristics of strain B18 and its 16S DNA gene sequence were identified, and the biocontrol effect of strain B18 was assessed in pot and field tests, as well as in a field-control test. Drilling methods were used to determine the antibacterial activity of metabolites from strain B18 and their effects on the growth of pathogen mycelia and spores. The best bacteriostatic rate was 85.4%. B18 can hydrolyse starch and oxidize glucose but does not produce gas; a positive result was obtained in a gelatine liquefaction test. According to 16S DNA gene sequencing, strain B18 is Bacillus methylotrophicus (GenBank accession number: MG457759). The results of pot and field-control trials showed 98% disease control when inoculating $10^8cfu/ml$ of the strain. The disease control effect of the B18 culture liquid (concentrations of $10^8$, $2{\times}10^6$, $10^6$, $5{\times}10^5$ and $2.5{\times}10^5cfu/ml$) in the field-control test was higher than 80%, and the cure rate of the original delivery solution was 96%. Therefore, in the practical forestry production, a $2.5{\times}10^5cfu/ml$ culture liquidshould be applied in advance to achieve good control effects.

• 대한한방체열의학회지
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• 제1권1호
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• pp.13-19
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• 2002

Objectives : This study was performed to apply thermography as an method in diagnosis of hwabyung patients. We studied 11 Hwabyung patients who visited to chronic diseases center and circulatory oriental internal medicine of Kangnam oriental medicine hospital and 11 patients control group. Methods : Diagnosis of Hwabyung was based on the dignostic criteria of Hwabyung. The temperature was measured on Chonjung(CV17) Shimsu(B15), Kansu(B18), Kyonjong(G21) in each group. The ${\Delta}T$ was measured between Chonjung(CV17) and Chungjong(CV16), left and right Chungjong(CV16), Shimsu(B15), Kansu(B18), Kyonjong(G21) in each group. We compared the ${\Delta}T$ and DITI types between patients and control group. Results : The ${\Delta}T$ between left and right Chungjong(CV16), Shimsu(B15), Kansu(B18), Kyonjong(G21) were not statistically significant. But the ${\Delta}T$ between Chonjung(CV17) and Chungjong(CV16) was statistically significant(P<0.05) in each group. In control group, DITI type was straight 36%, diamond 27%, multiple small spot 18%, others 18%. In Hwabyung patients group, DITI type was inverse triangle 64%, multiple small spot 9.1%, round 9.1%. Conclusions : The ${\Delta}T$ between Chonjung(CV17) and Chungjong(CV16) and DITI type is considered useful diagnostic methods on Hwabyung patients.

• 한국전자파학회논문지
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• 제18권2호
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• pp.105-110
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• 2007

본 논문에서는 $6{\sim}18$ GHz의 넓은 대역에서 진폭 선형성을 얻기 위해, 인접 전송선-스터브 블록 간에 저항이 삽입된, 마이크로스트립형의 선형 진폭 등화기가 구현된다. 선형성과 반사 손실 특성을 만족하도록 저항과 스터브의 값들이 변화하며, 적합한 수치를 찾으면서 설계가 이뤄진다. 실험치는 본 구현 결과는 $6{\sim}18$ GHz에서 요구되는 약 9 dB의 선형도와 약 10 dB 이하의 반사 손실이 얻어지고, 설계 기법의 타당함을 보인다.

• IMMUNE NETWORK
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• 제3권3호
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• pp.176-181
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• 2003

Background: The molecular basis of follicular dendritic cells (FDC)-germinal center (GC) B cell interaction is largely unknown, although this cellular interaction is thought to be important for the whole process of GC B cell differentiation. Methods: Using FDC-like cells, HK, and highly purified GC B cells, we attempted to identify the molecules that play critical roles in the interactions between FDC and B cells. GC B cells were co-cultured with HK cells and soluble CD154 in the presence or absence of various function-blocking monoclonal antibodies to examine their effect on GC B cell binding to HK cells and B cell proliferation. Results: Anti-CD11a and anti-CD54 antibodies inhibited GC B cell binding to HK cells while anti-CD49d and anti-CD106 antibodies did not. GC B cell proliferation was not impaired by the disruption of GC B cell-HK cell adherence. Conclusion: Our results suggest that CD11a/CD18-CD54 interactions play an important roles in the initial binding of GC B cells to FDC and diffusible growth factors from FDC may be responsible the massive proliferation of GC B cells.

• 한국식품저장유통학회지
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• 제22권4호
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• pp.545-552
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• 2015

본 연구는 된장 제조 시 자연발효 시킨 control과, B. subtilis KACC15935, B. subtilis HJ18-9균주를 starter로 접종하여 발효시킨 팽화미된장의 효소활성과 품질특성을 측정하였다. 환원당을 유리하는데 관여하는 ${\alpha}$-amylase 효소활성의 경우 control과 HJ18-9를 접종한 시료에서 높게 나타났다. 또한 된장의 단백질을 분해하여 특유의 구수한 맛 성분을 유리하는 protease 활성의 경우도 control과 HJ18-9를 접종한 시료에서 높게 나타났으며, 이는 아미노태질소 함량에서도 같은 경향을 나타냈다. 또한 cellulose를 분해할 수 있는 능력을 가지고 있어 유용성분의 장내 이용성 증진을 위해 널리 사용되는 효소인 cellulase활성이 있는 HJ18-9균주를 처리한 접종구에서 $115.45{\pm}30.05unit/g$로 control과 B. subtilis KACC15935 처리구에서 $53.75{\pm}15.91$, $43.75{\pm}13.51unit/g$ 인 것에 비해 높게 나왔다. 이러한 결과로 본 연구를 통해 선별한 균주를 스타터로 접종하여 된장 제조에 알맞은 균주를 개발, 평가하여 가공품으로 개발의 기초연구가 되고자 하였다.

• 한국항행학회논문지
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• 제18권4호
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• pp.359-363
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• 2014

본 논문은 출력 포트 사이의 물리적인 분리와 전기적 격리도를 개선시킨 비대칭 분배기기의 설계와 성능을 나타낸 것이다. 이 분배기는 입력 포트와 연결된 두 개의 ${\lambda}/4$ 전송선로의 중앙에 격리소자 저항 $18{\Omega}$과 캐패시터 0.7 pF를 직렬로 연결하여 출력 포트 사이에 격리도를 높였고, 출력 포트가 다른 회로에 연결이 쉽도록 하는 설계 방법을 제시하였다. 이러한 설계는 출력포트의 격리도를 물리적으로 향상시켜주며, 이 분배기와 다른 회로가 연결 될 때 별도의 전송선로가 필요 없이 연결할 수 있는 특징을 갖고 있다. 이러한 특성을 확인하기 위하여 중심 주파수 2 GHz에서 4:1 비대칭 분배기를 설계하였고 측정된 결과는 반사계수가 17 dB 이상, 삽입손실은 1.5 dB 와 7.7 dB, 그리고 격리도는 18 dB 이상 특성을 얻었다. 이러한 전기적 특성은 시뮬레이션 결과와 일치함을 확인하였다.

• Bulletin of the Korean Chemical Society
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• 제18권11호
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• pp.1144-1145
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• 1997

• 원예과학기술지
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• 제34권2호
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• pp.294-304
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• 2016

Members of the genus Brassica, which are known as oil crops or cruciferous vegetables, are widely cultivated in Canada, Australia, Asian and Europe. Because Brassica species have high yields, are well adapted to their environments, and are self-incompatible, the germplasm is abundant. Previous studies have reported abundant genetic diversity even within Brassica subspecies. In Korea, fresh cabbage leaves are eaten with roast meat, and to meet the current popular demand, new varieties are being increasingly bred. To determine the genetic diversity and relationships among the cabbage vegetables in Korea, we evaluated the genetic variation of 18 accessions based on 5S and 18S ribosomal RNA (rRNA) gene sequences. We detected many variable nucleotide sites, especially in the 5S rRNA gene sequences. Because the length of the 18S rRNA gene might influence the dissimilarity rate statistics, we used both the 5S and 18S sequences to analyze the phylogenetic relationships. S7 (B. oleracea) showed the most distant phylogenetic relationship with the other Brassica species. Interestingly, B2 (B. oleracea), B15, and B18 (B. campestris) have three different types of leaf profiles, and were divided into one group, and the other Brassica species formed another group. Statistical analysis of interspecies and intraspecies genetic distances revealed that B. campestris L. showed higher genetic diversity than B. oleracea L. This work provides additional data that facilitates the evaluation of the genetic variation and relationships among Brassica species. The results could be used in functional plant breeding programs to improve Brassica crops.

• Journal of Microbiology and Biotechnology
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• 제10권3호
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• pp.399-404
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• 2000

The adjuvant effect of a muramyl dipeptide (MDP) derivative, MDP-Lys(L18), on enhancing of antitumor immunity induced by X-irradiated tumor cells against highly metastatic B16-BL6 melanoma cells was examined in mice. Mice immunized intradermally (i.d.) with a mixture of X-irradiated B16-BL6 cells and MDP-Lys (L18) [Vac+MDP-Lys (L18)] followed by an intravenous (i.v.)inoculation of $10^4$ viable tumor cells 7 days after immunization, showed a significant inhibition of experimental lung metastasis of B16-BL6 melanoma cells. The most effective immunization for the prophylactic inhibition of tumor metastasis was obtained from the mixture of $100{\;}\mu\textrm{g}$ of MDP-Lys (L18) and $10^4$ X-irradiatied tumor vaccine. Furthermore, immunization of mice with Vac+MDP-Lys(L18), 3 days after tumor challenge, resulted in a significant inhibition of lung metastasis of B16-BL6 melanoma cells in an experimental lung metastasis model. Similarly, the administration of Vac+MDP-Lys(L18), 1 or 7 days after tumor removal, markedly inhibited tumor metastasis of B16-BL6 in a spontaneous lung metastasis model. When Vac+MDP-Lys (L18) was i.d. administered 3 days after subcutaneous (s.c.) inoculation of tumor cells ($5{\times}10^5/site$) on the back, mice treated with Vac+MDP-Lys(L18) showed inhibition of significantly tumor growth on day 20. These results suggest that MDP-Lys (L18) is able to enhance antitumor activity induced by X-irradiated tumor vaccine to reduce lung metastasis of tumor cells, and is a potent immunomodulating agent which may be applied prophylactically as well as therapeutically to treatment of cancer metastasis.

• 대한화학회지
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• 제43권4호
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• pp.447-455
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• 1999

Allyl (5R)-dibromopenicillanate(6)의 2,3-O-isopropylidene-D-glyceraldehyde와의 축합, $Zn-NH_4OAc$에 의한 환원 및 Mitsunobu 제거반응의 일련의 과정을 거처서 allyl (5R)-(Z) 및 (5R)-(E)-6-[(2S)-2,3-isopropylidenedioxypropylidene]penicillanate(10a 및 10b)를 합성하였다. 화합물10a의 이소프로필리덴 및 알릴 보호기를 제거하여 potassium (5R)-(Z)-6-[(2S)-2,3-dihydroxypropylidene]penicilla-nate(4)를 얻었다. 그러나 화합물 10b의 이소프로필리덴 보호기를 제거한 결과 ${\alpha},\;{\beta}$-unsaturated-lactone(12)이 얻어짐을 확인하였다. Allyl(5R)-6,6-dibromopenicillanate(6)의 (2S)-2-(t-butyldimethyl-silyloxy)propanal(15)과의 축합, $Zn-NH_4OAc$에 의한 환원 및 Mitsunobu 제거반응 또는 메실화-제거반응의 일련의 과정을 거처서 allyl (5R)-(Z)- 및 (5R)-(E)-6-[(2S)-2-(t-butyldimethylsilyloxy)propylidene]-penicillanate(18a 및 18b)를 합성하였다. 화합물 18a 및 18b의 t-부틸디메틸실릴 및 알릴 보호기를 제거하여 potassium (5R)-(Z)- 및 (5R)-(E)-6-[(2S)-2-hydroxypropylidene]penillanate(5a 및 5b)를 얻었다.