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Decay Damage of Japanese Larch(Larix leptolepis) Caused by Two Butt-Rot Fungi, Phaeolus schweinitzii and Sparassis crispa (해면버섯균(菌) 꽃송이버섯균(菌)에 의(依)한 낙엽송 생입목(生立木)의 심재부후피해(心材腐朽被害))

  • Kim, Hyun Joong;Kim, Jun Suob;Yi, Chang Keun
    • Journal of Korean Society of Forest Science
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    • v.79 no.2
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    • pp.138-143
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    • 1990
  • In mature stand of Japanese larch (Larix leptolepis(S. et Z.) Gordon) planted in 1925, heartrot damage was investigated by felling some infected trees with fruit bodies of two butt-rot fungi, Phaeolus schweinitzii and Sparassis crispa. Percentage of heartrot area on basal area of decay trees was 19.2-74.4, and the height reached by the decay was 1.2-5.5m. Percentage of decay volume was in the range of 2.2-13.4%, but the loss wood to the height reached by the decay amounted to 27.2% (range 13.1-37.1%) of whole volume, Decay damage caused by S. crispa was much greater than that caused by P. schweinitzii. Compressive strength ($52-77kg/cm^2$) and specific gravity (0.40-0.47) of rot wood was reduced to 77% and 39% respectively in maximum as compared to sound wood of healthy tree, and those of sound-looking wood close by heartrot to 42% and 26%. The current annual growth of trees with decay was fallen behind that of sound tree from eight to fourteen years after planting in D. B. H. and from twelve to twenty-one years after in volume.

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Effects of 17β-Estradiol on Colonic Permeability and Inflammation in an Azoxymethane/Dextran Sulfate Sodium-Induced Colitis Mouse Model

  • Song, Chin-Hee;Kim, Nayoung;Sohn, Sung Hwa;Lee, Sun Min;Nam, Ryoung Hee;Na, Hee Young;Lee, Dong Ho;Surh, Young-Joon
    • Gut and Liver
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    • v.12 no.6
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    • pp.682-693
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    • 2018
  • Background/Aims: Intestinal barrier dysfunction is a hallmark of inflammatory bowel diseases (IBDs) such as ulcerative colitis. This dysfunction is caused by increased permeability and the loss of tight junctions in intestinal epithelial cells. The aim of this study was to investigate whether estradiol treatment reduces colonic permeability, tight junction disruption, and inflammation in an azoxymethane (AOM)/dextran sodium sulfate (DSS) colon cancer mouse model. Methods: The effects of $17{\beta}$-estradiol (E2) were evaluated in ICR male mice 4 weeks after AOM/DSS treatment. Histological damage was scored by hematoxylin and eosin staining and the levels of the colonic mucosal cytokine myeloperoxidase (MPO) were assessed by enzyme-linked immunosorbent assay (ELISA). To evaluate the effects of E2 on intestinal permeability, tight junctions, and inflammation, we performed quantitative real-time polymerase chain reaction and Western blot analysis. Furthermore, the expression levels of mucin 2 (MUC2) and mucin 4 (MUC4) were measured as target genes for intestinal permeability, whereas zonula occludens 1 (ZO-1), occludin (OCLN), and claudin 4 (CLDN4) served as target genes for the tight junctions. Results: The colitis-mediated induced damage score and MPO activity were reduced by E2 treatment (p<0.05). In addition, the mRNA expression levels of intestinal barrier-related molecules (i.e., MUC2, ZO-1, OCLN, and CLDN4) were decreased by AOM/DSS-treatment; furthermore, this inhibition was rescued by E2 supplementation. The mRNA and protein expression of inflammation-related genes (i.e., KLF4, NF-${\kappa}B$, iNOS, and COX-2) was increased by AOM/DSS-treatment and ameliorated by E2. Conclusions: E2 acts through the estrogen receptor ${\beta}$ signaling pathway to elicit anti-inflammatory effects on intestinal barrier by inducing the expression of MUC2 and tight junction molecules and inhibiting pro-inflammatory cytokines.

Synthesis of Binuclear Bismacrocyclic Iron(II) Complex by the Aerobic Oxidation of Iron(II) Complex of 1,4,8,11-Tetraazacyclotetradecane

  • Myunghyun Paik Suh;Gee-Yeon Kong;Il-Soon Kim
    • Bulletin of the Korean Chemical Society
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    • v.14 no.4
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    • pp.439-444
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    • 1993
  • The aerobic oxidation of the Fe(II) complex of 1,4,8,11-tetraazacyclotetradecane, [Fe(cyclam)$(CH_3CN)_2](ClO_4)_2$, in MeCN in the presence of a few drops of $HClO_4$ leads to low spin Fe(III) species [Fe(cyclam)$(CH_3CN)_2](ClO_4)_3$. The Fe(III) cyclam complex is further oxidized in the air in the presence of a trace of water to produce the deep green binuclear bismacrocyclic Fe(II) complex $[Fe_2(C_{20}H_{36}N_8)(CH_3CN)_4](ClO_4)_4{\cdot}2CH_3CN$. The Fe(II) ions of the complex are six-coordinated and the bismacrocyclic ligand is extensively unsaturated. $[Fe_2(C_{20}H_{36}N_8)(CH_3CN)_4](ClO_4)_4{\cdot}2CH_3CN$ crystallizes in the monoclinic space group $P2_1/n$ with a= 13.099 (1) ${\AA}$, b= 10.930 (1) ${\AA}$, c= 17.859 (1) ${\AA}$, ${\beta}$= 95.315 $(7)^{\circ}$, and Z= 2. The structure was solved by heavy atom methods and refined anisotropically to R values of R= 0.0633 and $R_w$= 0.0702 for 1819 observed reflections with F > $4{\sigma}$ (F) measured with Mo K${\alpha}$ radiation on a CAD-4 diffractometer. The two macrocyclic units are coupled through the bridgehead carbons of ${\beta}$-diimitie moieties by a double bond. The double bonds in each macrocycle unit are localized. The average bond distances of $Fe(II)-N_{imine}$, $Fe(II)-N_{amine}$, and $Fe(II)-N_{MeCN}$ are 1.890 (5), 2.001 (5), and 1.925 (6) ${\AA}$, respectively. The complex is diamagnetic, containing two low spin Fe(II) ions in the molecule. The complex shows extremely intense charge transfer band in the near infrared at 868 nm with ${\varepsilon}$= 25,000 $M^{-1}cm^{-1}$. The complex shows a one-electron oxidation wave at +0.83 volts and two one-electron reduction waves at -0.43 and-0.72 volts vs. Ag/AgCl reference electrode. The complex reacts with carbon monoxide in $MeNO_2$ to form carbonyl adducts, whose $v_{CO}$ value (2010 $cm^{-1}$) indicates the ${\pi}$-accepting property of the present bismacrocyclic ligand.

In Vivo Development of Mouse IVF/IVC Embryo Treated with Epidermal Growth Factor (EGF) (EGF 처리를 받은 체외생산된 생쥐배의 체내 발달)

  • Kim, E.Y.;Kim, M.K.;Yi, B.K.;Lee, H.S.;Yoon, S.H.;Park, S.P.;Chung, K.S.;Lim, J.H.
    • Clinical and Experimental Reproductive Medicine
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    • v.24 no.2
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    • pp.261-265
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    • 1997
  • The objective of this study was to examine the effect of EGF to the in vivo development of mouse IVF/IVC embryo. The 2-cell embryos were cultured in medium (5-6 embryos/25 ${\mu}l$/drop) w/wo EGF (10 ng/ml) and day 4 blastocysts recovered from each treatment group were transferred into the uteri of recipients of pseudopregnant day 3. The results obtained in this experiment were summarized as follows: 1. When the effect of EGF to the in vitro development and cell number of blastocysts produced from the culture of 2-cell embryos in w/wo EGF was determined, those results of EGF treatment group showed not significant difference compared with control. 2. However, when the effect of EGF to the in vivo development of blastocysts recovered from each treatment group was examined, production of the normal fetus against transferred embryos in EGF treatment group (51.2%) was very higher than that in control group (31.1%), although total implantation was not significantly different between treatment group (control: 64.4%, EGF: 69.8%). Therefore, this result suggested that EGF can affect to the in vivo development of IVF/IVC embryos through the improvement of embryo quality, although EGF treated embryos showed not significant development rate compared with control.

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Investigation for Processing Conditions of Porridge with Addition of Ramie Leaf (Boehmeria nivea L) Powder Using a Response Surface Methodology (반응표면 분석법을 이용한 모시분말 첨가 비단죽 제조조건 조사)

  • Lee, Young-Tack;Im, Ji-Soon
    • Food Science and Preservation
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    • v.19 no.6
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    • pp.841-848
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    • 2012
  • Response surface methodology was applied to the processing conditions to investigate the physicochemical properties of ramie porridge. In the processing conditions based on the central composite design with the addition of ramie leaf powder (0-4 g) and water (700-1100 g), the coefficients of determination (R2) of the models were above 0.9141 for the physicochemical properties, above 0.7627 for the Hunter color parameters, and above 0.8975 for the rheological properties. The soluble solid content, viscosity, and gumminess of the sample decreased significantly with an increase in the water added, whereas the spreadability and adhesiveness increased significantly. The Hunter color L value of the sample decreased significantly with an increase in the amount of ramie leaf powder added, whereas the Hunter b value increased significantly. The estimated maximum viscosity of the ramie porridge was shown to be 24,643 cp when 1.90 g ramie leaf powder and 700.24 g water were added; the estimated maximum lightness of the ramie porridge was shown to be 59.11 when 0.02 g ramie leaf powder and 869.30 g water were added; and the estimated maximum gumminess of the ramie porridge was shown to be 21.46 g when 2.12 g ramie leaf powder and 700.35 g water were added.

Effects of Roasted Korean Oak Chip Addition on the Aging of Red Wine (국산 참나무편의 첨가가 적포도주의 숙성에 미치는 영향)

  • Lee, A-Rong;Park, Heui-Dong
    • Food Science and Preservation
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    • v.18 no.6
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    • pp.891-897
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    • 2011
  • Oak barrel have been used for a long time for the aging of wine. Instead of oak barrel aging, however, roasted oak chips can be directly added in the wine before aging. In this study, Korean oak chips roasted for different time durations were used for the red wine aging, and the properties of wine aged through the addition of the roasted oak chips were characterized. No significant differences were observed in the alcohol, soluble-solid, reducing-sugar and total-acid contents based on the oak chips aging, but the total phenol content increased. The methanol, ethyl acetate, propanol, isobutanol and isoamyl alcohol contents also increased. Especially, the methanol and propanol contents increased as the roasting time was prolonged. Although the hue values were similar to one another, the intensity as well as the lightness, redness, and yellowness values were high in the wine aged with the roasted oak chips. In the sensory evaluation, the wine aged with oak chips roasted for 2 h showed the highest scores in the flavor, taste, and overall preference.

Quality Characteristics of Canned Boiled Oyster and Canned Boiled Oyster in Bamboo Salt in Various Sterilization Conditions (살균조건에 따른 굴 보일드통조림 및 죽염 굴 보일드통조림의 식품 품질 특성)

  • Kong, Cheong-Sik;Je, Hae-Soo;Jung, Jae-Hun;Kwon, Soon-Jae;Lee, Jae-Dong;Yoon, Moon-Joo;Choi, Jong-Duck;Kim, Jeong-Gyun
    • Journal of Fisheries and Marine Sciences Education
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    • v.26 no.6
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    • pp.1231-1244
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    • 2014
  • Oysters, especially are excellent source of several mineral including iron, zinc and selenium, which are often low in the mordern diet. They are also an excellent source of glycogen, vitamin B12 and considered the healthiest when eaten raw on the half shell in good tasted season from November to March. This study was investigated for the purpose of obtaining basic data which can be applied to processing of two kinds of canned boiled oyster (canned boiled oyster, canned boiled oyster in bamboo salt). Shucked oyster meat was cooked in steam (15min) after washing with water, filled 90g into can (301-3), added with salt solution and then precooked for 10 min. at $100^{\circ}C$. Canned boiled oyster was added 1.5% salt solution 60mL. Canned boiled oyster in bamboo salt was added 0.5% salt solution 30mL and 0.7% bamboo salt solution 30mL. The cans were seamed using a vacuum seamer, and then sterilized for various Fo values (Fo 8~12 min.) in a steam system retort at $116^{\circ}C$, $118^{\circ}C$. Viable bacterial count, proximate composition, pH, salinity, yield, VBN, amino-N, TBA, mineral, color value, free amino acid, hardness and sensory evaluation of two kinds of canned boiled oyster produced at various sterilization condition (Fo 8~12 min.) were measured after divide to meat and juice. The results showed that canned boiled oyster in bamboo salt sterilized at Fo 8 min. was the most desirable because this condition is the most economical and tasty.

The Relationship between the Personality of Elementary School Students and the Safety Accidents in the School (초등학생의 성격특성과 학교안전사고)

  • Kim, Chang-Hee;Park, Young-Soo
    • The Journal of Korean Society for School & Community Health Education
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    • v.4
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    • pp.1-20
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    • 2003
  • This study was not intended to analyze out the realities of the safety accidents in school. The purpose of this study was to find out the personality of elementary school students in 6th grade and analyze the relationship between the personality of elementary school students and the safety accidents in the school. We made a visit 12 elementary school personally in the B city of Gyeonggi Province and gave out 552 questionaries in order to accomplish the purpose of this study. And we analyzed the 501 answer data except the unfittable data 51 sheets-in case of no answer or untruthfulness. The inspection of the personality used the Eysenck Personality Questionnaire(EPQ) and the questionnaires of the safety accidents in the school were reorganized of the questionnaires which were used in Chun-Ok, Joen(1997) and One-Yong, Kim(1999)'s study to correspond with our purpose of study. Answer sheets were gone through the t and x2 test by using the program of SPPS. First, elementary school students are in the formative period in the life of a man. So many students's personality was in the middle group rather than in high and low group. In regard of the distintion between the boy students and the girl students, the boy students had higher score in Psychoticism(P) but the girl students had higher score in Neutroticism(N). And Lie(L) was showing a little more score in the girl students. Second, there was significant difference in the investigation of safety accidents in school according to sex distinction. 1) Comparing the boy students and the girl students, the rate of accidents was higher in boy rather than girl. And there was significant difference. 2) Comparing the grade of the school, the rate of accidents was higher in upper grade than lower grade both girls and boys. 3) In the kind of accidents, the traumatic Injuries were very popular and the next were contusions. There was significant difference in the traumatic injury and nosebleed. 4) The results of injured parts showed that the injuries of foot, leg, hand, arm hold a large majority. And there was a significant difference in head, face, foot, leg and etc. 5) Most accidents broke out during the break time and then accidents happened after school were the next. The flowings were lunch time and class time. There was a significant difference in accidents happened during the class, break time and lunch time. 6) Comparing the subjects, the most accidents broke out in the class of physics. 7) Among the cause of accidents, one's own carelessness was showing the highest rate. There was a significant distintion in the fight and game. 8) The safety accidents took place most in playground, and there was a significant difference. 9) Being slight wound was showing almost in the degrees of damage. And there was a significant difference. Third, the boy students were not showing the significant difference in relation to the personality, the frequency of accidents and the degrees of damage. But the girl students were showing the significant difference in relation to the Extraversion-Introversion(E) and the frequency of accidents. And there were a significant difference between E and N. Also they were showing the intense damages.

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Studies on the Characteristics of Humic Substances and Fractionations of Nitrogen in Paddy Soils (답토양(畓土壤)의 부식(腐植)과 질소형태(窒素形態)에 관(關)한 연구(硏究))

  • Kim, Young-Sig;Whang, Kwang-Nam;Kim, Weon-Chul;Park, Moon-Hee;Kim, Kwang-Sik
    • Korean Journal of Soil Science and Fertilizer
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    • v.19 no.3
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    • pp.239-244
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    • 1986
  • This study was conducted to investigate the characteristics of humic substances and the fractional distribution of organic nitrogen in Korean paddy soils. The results are obtained as follows: 1. The content of humus in soils used was 665-2680 mg/100g and the average contents of humic acid in normal paddy soils and in sandy paddy soils were 1436 mg/100g, 970 mg/100g respectively. 2. The humic acid diagrams classed by Kumada method belong to B type, P type and Rp type. 3. The content of Mineral - N to Total - N was 1.04-2.86% and the average contents of that in sandy paddy soils and in normal paddy soils were 1.72% and 1.75% respectively. 4. The fractionations of acid hydrolysable organic - N in sandy paddy soils were Amino acid - N (31.52%), Humin - N (20.63%), Amino sugar - N (18.70%), and Unknown - N (25.73%).

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The Raw silk Size-Deviation Relationship with the Cocoon cultured in Korea (한국산 가잠계 견사섬도가 생사섬도 편차에 미치는 영향)

  • 최병희;김동욱
    • Journal of Sericultural and Entomological Science
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    • v.3
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    • pp.61-67
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    • 1963
  • This report is prepared to find how the filament of cocoon bave size deviation relates with the raw silk made by them which are intensively cultured in this country. Three recommended hybrid varieties and two varieties under working at Suwon Sericultural Experiment Station were selected as specimens. The cocoons were reeled as an individual filament of every fifty meters long skein with a wrap reel to weigh the denier and to investigate the relationship of the above statement so that it may be used for the quality estimation before processing it into raw silk. The conclusions obtained are as follows. (1) The variation of Pk${\times}$Sn was found as best cocoon for 21 denier raw silk use, but the number of cocoon to make the denier has to be eight which might cause more labor cost. (2) Baektoo-Kumkang and Myohiang-Chongchon were found as economical varieties for 21 denier use. (3) Seulak-Soyang is a proper variety for the use of 14 or 28 denier silk use. (4) Myohiang-Chongchon did not confirm a good property from the aspect of denier deviation. (5) It was found that there was a fairly strong corelationship between the mean cocoon bave size deviation as indicated by Ono's report. (6) Three graphs were prepared to estimate the cocoon quality before processing into various sizes of raw silk using the mean cocoon bave size and the raw silk size to be prepared. (7) Mean time, the graph which is able to estimate the expectable grade of the raw silk size deviation was designed for the practical use. (8) The expectable grade of the varieties used in this report were found as following data. Notice (A......cocoon number to make raw silk (B......total cocoon bave size deviation (C......expectable silk grade (9) The result of the work concerning the expectable denier deviation on 21 denier silk was the same with the statistical actual testing result as 1.25 D while the distribution showed farther necessity of improvement in technically.

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