• 한국제4기학회지
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• 제15권2호
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• pp.75-81
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• 2001

한반도 내륙지역의 제4기 후기의 식생변천 과정을 밝히기 위하여, 충북 청원군 옥산면 소로리 유적지($36^{\circ}41'3"N$, $127^{\circ}24'49"E$)의 퇴적물을 대상으로 화분분석을 실시하였다. 그 결과, 이 지역 일대는 \circled1OS-1의 아고산성 침엽수림(만빙기;약 15,000yr.B.P.~10,000yr.B.P.)$\longrightarrow$\circled2OS-2의 침엽ㆍ낙엽 활엽수혼합림(후빙기 초기;약 10,000yr. B.P.경)$\longrightarrow$\circled3OS-3의 냉온대성 낙엽활엽수림(후정기 중기;약 10.000yr.B.P.~2,000yr.B.P.)$\longrightarrow$\circled4OS-4의 침엽수림(후빙기 후기;약 2,000yr.B.P.) 이후)로 변천하였음을 알 수 있었다.을 알 수 있었다.

• Animal cells and systems
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• 제12권2호
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• pp.69-75
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• 2008

The tumor suppressor and transcription factor p53 is a key modulator of cellular stress responses, and can trigger apoptosis in many cell types including neurons. In this study, we have shown that Microtubule-associated protein 1B(MAP1B) light chain interacts with tumor suppressor p53. MAP1B is one of the major cytoskeletal proteins in the developing nervous system and essential in forming axons during elongation. We also demonstrate that both p53 and MAP1B-LC1 interact in the nucleus in HEK 293 cells. Indeed, we show that the MAP1B-LC1 negatively regulates p53-dependent transcriptional activity of a reporter containing the p21 promoter. Consequently, MAP1B light chain binds with p53 and their interaction leads to the inhibition of doxorubicin-induced apoptosis in HEK 293 cells. Furthermore, these examinations might be taken into consideration when knock-down of MAP1B-LC1 is used as a cancer therapeutic strategy to enhance p53's apoptotic activity in chemotherapy.

• 약학회지
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• 제42권4호
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• pp.414-421
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• 1998

In order to compare the suppressive effect of quercetin and its glycosides, such as quercitrin (quercetin-3-rhamnoside), isoquercitrin (quercetin-3-glucoside), hyperin (querceti n-3-galactoside)and rutin (quercetin-3-rhamnosyl glucoside), on the genotocicity by benzo(a)pyrene(B(a)P), in vitro sister chromatid exchange(SCE) test using mouse spleen lymphocytes and in vivo micronucleus test using mouse peripheral blood were performed. B(a)P-induced SCEs in vitro were slightly decreased by the simultaneous treatment of quercetin and its glycosides, although there was no significant decrease. On the other hand, MNU induced micronucleated reticulocytes(MNRL7s) in vivo were significantly decreased with a dose-dependent manner in all compounds tested. However, there were no differences between quercetin aglycone and glycosides in the suppressive effects under experimental condition of this study. To elucidate, the action mechanism of quercetin aglycone and its glycosides against B(a)P-induced genotoxicity, the assay of DNA binding with B(a)P was studied. Quercetin aglycone and its glycosides inhibited B(a)P metabolism in the presence of S-9 mix and decreased the B(a)P/DNA binding in the calf thymus DNA with S-9 mix. These results suggest that antigenotoxicity of quercetin antiglycosides on B(a)P-induced genotoxicity is due to decrease of DNA binding with B(a)P through the inhibition of metabolism with B(a)P in the calf thymus DNA. Therefore, quercetin and its glycosides may act as an antigenotoxicity agent and may be useful as a chemopreventive agent of polycyclic aromaic hydrocarbons like B(a)P.

• 열처리공학회지
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• 제15권2호
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• pp.76-81
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• 2002

The melt-spun amorphous $Fe_{77-X}P_{13}C_4B_4Al_2Mo_X$(x=4~10) and $Fe_{82-X}P_XC_4B_4Al_2Mo_8$(x=9~15) alloys were found to exhibit a large supercooled liquid region(${\Delta}T_x$) exceeding 40 K before crystallization. The largest ${\Delta}T_x$ for the glassy alloys containing Mo reaches as large as 65 K for the $Fe_{69}P_{13}C_4B_4Al_2Mo_8$ alloy. The corrosion behavior of the amorphous $Fe_{77-X}P_{13}C_4B_4Al_2Mo_X$(x=4~15) and $Fe_(82-X)P_XC_4B_4Al_2Mo_8$ (x=9~17) alloys were examined by electrochemical measurements in 9M $H_2SO_4$ solution at 303 K. The addition of Mo(or P) for replacing some portion of Fe is effective in improving the corrosion resistance of the investigated Fe-based glassy alloys. They are spontaneously passivated and have a wide passive region with low passive current density.

• 대한환경공학회지
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• 제31권12호
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• pp.1081-1088
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• 2009

Rhodamine B (RhB)의 제거를 위해 철 전극을 이용한 전기응집 (EC) 공정의 성능에 대해 연구하였다. RhB 제거 효율에 대한 전극 물질 (알루미늄, 철), 전류밀도, NaCl 첨가량, 초기 pH 및 초기 염료 농도와 같은 운전인자의 영향을 연구하였다. 이들 운전 인자에 대한 최적 운전 범위는 실험을 통하여 결정되었다. 희생 전극 물질로서 철 전극의 성능이 알루미늄 전극보다 우수하였다. 최적 전기분해 시간, 전류밀도, NaCl 첨가량 및 pH는 각각 10 분, 1630 A/$m^2$, 4 g/L 및 중성의 pH 이었다. 최적의 공정 조건하에서 RhB 농도가 230 mg/L인 조건에서 RhB는 효과적으로 제거되었고 (> 93.4%), RhB의 COD도 역시 감소하였다(> 88.9%). RhB의 색도와 COD 제거에 대한 상기 조건에서의 전기 에너지 소비량은 각각 10.3 and 10.8 kWh/kg RhB으로 나타났다. 전기응집 공정은 RhB와 같은 염색폐수 처리에 효과적인 것으로 나타났다.

• Molecules and Cells
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• 제41권5호
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• pp.423-435
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• 2018

This investigation was aimed at working out the combined role of lncRNA H19, miR-29b and Wnt signaling in the development of colorectal cancer (CRC). In the aggregate, 185 CRC tissues and corresponding para-carcinoma tissues were gathered. The human CRC cell lines (i.e. HT29, HCT116, SW480 and SW620) and normal colorectal mucosa cell line (NCM460) were also purchased. Si-H19, si-NC, miR-29b-3p mimics, miR-29b-3p inhibitor, si-PGRN and negative control (NC) were, respectively, transfected into the CRC cells. Luciferase reporter plasmids were prepared to evaluate the transduction activity of $Wnt/{\beta}-catenin$ signaling pathway, and dual-luciferase reporter gene assay was arranged to confirm the targeted relationship between H19 and miR-29b-3p, as well as between miR-29b-3p and PGRN. Finally, the proliferative and invasive capacities of CRC cells were appraised through transwell, MTT and scratch assays. As a result, overexpressed H19 and down-expressed miR-29b-3p displayed close associations with the CRC patients' poor prognosis (P < 0.05). Besides, transfection with si-H19, miR-29b-3p mimic or si-PGRN were correlated with elevated E-cadherin expression, decreased snail and vimentin expressions, as well as less-motivated cell proliferation and cell metastasis (P < 0.05). Moreover, H19 was verified to directly target miR-29b-3p based on the luciferase reporter gene assay (P < 0.05), and miR-29b-3p also bound to PGRN in a direct manner (P < 0.05). Finally, addition of LiCl ($Wnt/{\beta}-catenin$ pathway activator) or XAV93920 ($Wnt/{\beta}-catenin$ pathway inhibitor) would cause remarkably altered E-cadherin, c-Myc, vimentin and snail expressions, as well as significantly changed transcriptional activity of ${\beta}-catenin/Tcf$ reporter plasmid (P < 0.05). In conclusion, the lncRNA H19/miR-29b-3p/PGRN/Wnt axis counted a great deal for seeking appropriate diagnostic biomarkers and treatment targets for CRC.

• Plant Biotechnology Reports
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• 제2권1호
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• pp.1-6
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• 2008

Cotyledonary leaves of tomato cv. Megha were transformed with the hepatitis B virus 's' gene, which encodes surface antigen. Six plant expression cassettes (pHBS, pHER, pEFEHBS, pEFEHER, pSHER and pEFESHER) were used to assay the possible expression levels by agroinfiltration. The maximum transient expression level of 489.5 ng/g D.W. was noted in pEFEHER-infiltrated cotyledonary leaves. Transgenic tomato plants with pEFEHBS and pEFEHER expression cassettes were regenerated and characterized by molecular analysis. The expression of the antigen in the fruits was confirmed by RT-PCR and ELISA analysis. This is the first report on the expression of hepatitis B surface antigen in tomato.

• 미생물학회지
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• 제26권4호
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• pp.291-297
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• 1988

신호배열 돌연변이인 rbsB 103는 전구체 리보스 결합단백질을 세포질내에 축적시키고, rbsB 106 복귀유전자는 이 전구체를 숙성 가능하게 하여 페리플라슴으로 수송되게 한다.(Iida el at., 1985, Park, el at., 1988). 본고에서는 rbsB 유전자의 세 allele, rbsB, rbsB 103와 rbsB 106를 이들이 코딩하는 단백질을 대량생산 하그l자 람다 P 프로모터 조절하에 클론하고 나아가서 다섯종의 단백질을 순수정제하였다.. 전구체 단백질의 pI는 8.0, 숙성단백질의 $P_{L}$는 7.5임을 밝혔다. 순수정제된 단백질의 아마노 말단의 아미노산 배열을 결정하여 DNA 염기서열로부터 밝혀진 아미노산 변화를 확인하였다.

• Molecules and Cells
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• 제45권3호
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• pp.122-133
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• 2022

The aim of this study was to investigating whether lncRNA H19 promotes myocardial fibrosis by suppressing the miR-29a-3p/miR-29b-3p-VEGFA/TGF-β axis. Patients with atrial fibrillation (AF) and healthy volunteers were included in the study, and their biochemical parameters were collected. In addition, pcDNA3.1-H19, si-H19, and miR-29a/b-3p mimic/inhibitor were transfected into cardiac fibroblasts (CFs), and proliferation of CFs was detected by MTT assay. Expression of H19 and miR-29a/b-3p were detected using real-time quantitative polymerase chain reaction, and expression of α-smooth muscle actin (α-SMA), collagen I, collagen II, matrix metalloproteinase-2 (MMP-2), and elastin were measured by western blot analysis. The dual luciferase reporter gene assay was carried out to detect the sponging relationship between H19 and miR-29a/b-3p in CFs. Compared with healthy volunteers, the level of plasma H19 was significantly elevated in patients with AF, while miR-29a-3p and miR-29b-3p were markedly depressed (P < 0.05). Serum expression of lncRNA H19 was negatively correlated with the expression of miR-29a-3p and miR-29b-3p among patients with AF (r_s = -0.337, r_s = -0.236). Moreover, up-regulation of H19 expression and down-regulation of miR-29a/b-3p expression facilitated proliferation and synthesis of extracellular matrix (ECM)-related proteins. SB431542 and si-VEGFA are able to reverse the promotion of miR-29a/b-3p on proliferation of CFs and ECM-related protein synthesis. The findings of the present study suggest that H19 promoted CF proliferation and collagen synthesis by suppressing the miR-29a-3p/miR-29b-3p-VEGFA/TGF-β axis, and provide support for a potential new direction for the treatment of AF.

• Korean Chemical Engineering Research
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• 제54권5호
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• pp.587-592
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• 2016

휴대용 고분자전해질 연료전지의 수소발생용으로써 $NaBH_4$는 많은 장점을 갖고 있다. 본 연구에서는 고농도 $NaBH_4$ 용액에서 비담지 Co-P-B, Co-B 촉매의 $NaBH_4$ 가수분해 특성에 대해 연구하였다. 고농도에서 수소 발생 수율을 높이기 위해 $NaBH_4$ 가수분해 반응의 수소 발생 수율에 미치는 촉매 형태, $NaBH_4$ 농도, 응축수 회수 등의 영향에 대해 실험하였다. Co-P-B 제조과정에서 붕소의 비가 높아질수록 수소 발생 수율이 증가하였다. Co-P:B = 1:5 촉매를 사용해 $NaBH_4$ 수용액 농도를 20 wt%에서 25 wt%로 증가시켰을 때 수소 발생 수율이 감소하였다. Co-P-B와 Co-B 촉매를 같이 사용한 반응기에서 촉매 팩의 두께를 감소시키고 응축수를 회수하여, $NaBH_4$ 25 wt% 수용액으로 최고 수소 발생수율 96.4%를 얻었다.