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Production and characterization of ultra-high-molecular weight poly(3-hydroxybutyrate) by recombinant Escherichia coli

  • Park, Jong-Pil;Park, Si-Jae;Lee, Sang-Yeop
    • 한국생물공학회:학술대회논문집
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    • 2001.11a
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    • pp.731-734
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    • 2001
  • An efficient fermentation strategy for the high level production of ultra-high-molecular weight poly(3-hdyroxybutyrate) (PHB) was developed. Although the cell and PHA concentrations obtained by flask cultures at different initial pH (6.0 or 6.9) were almost same level, the molecular mass of PHB produced were quite different along with the initial pH. When a recombinant Escherichia coli XL1-Blue harboring pJC2 containing the Alcaligenes latus PHB biosynthesis genes was cultivated in flask culture (pH 6.0), the PHB having a very high molecular weight of 22 MDa could be produced while only below 1 MDa at initial pH 6.9. The ultra-high-molecular weight PHB could be synthesized to high concentration of 89.8 g/L resulting in the PHB productivity of 2.07 g/L-h by simple fed-batch culture. In this study, we report that PHB having various molecular mass can be produced by employing metabolically engineered E. coli strains harboring the plasmids of different copy numbers containing the A. latus phbCAB genes.

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The Effect of Non-genetic Factors on Birth Weight and Weaning Weight in Three Sheep Breeds of Zimbabwe

  • Assan, N.;Makuza, S.M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.18 no.2
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    • pp.151-157
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    • 2005
  • Sheep production is affected by genetic and non-genetic factors. A knowledge of these factors is essential for efficient management and for the accurate estimation of breeding values. The objective of this study was to establish the non-genetic factors which affect birth weight and weaning weight in Dorper, Mutton Merino and indigenous Sabi sheep breeds. A total of 2,625 birth and weaning weight records from Grasslands Research Station collected from 1991 through 1993, were used. The records were collected from indigenous Sabi (939), Dorper (807) and Mutton Merino (898) sheep. A mixed classification model containing the fixed effects of year, birth status and sex was used for identification of non-genetic factors. Sire within breed was included as a random effect. Two factor interactions and three factor interactions were important in indigenous Sabi, Mutton Merino and Dorper sheep. The mean birth weights were 4.37${\pm}$0.04 kg, 4.62${\pm}$0.04 kg and 3.29${\pm}$0.04 kg for Mutton Merino, Dorper and Sabi sheep, respectively. Sire had significant effects (p<0.05) on birth weight in Mutton Merino and indigenous Sabi sheep. Year of lambing had significant effects (p<0.05) on birth weight in indigenous Sabi, Mutton Merino and Dorper sheep. The effect of birth status was non significant in Dorper and Mutton Merino sheep while effect of birth status was significant on birth weight in indigenous Sabi sheep. In Indigenous Sabi sheep lambs born as singles (3.30${\pm}$0.05 kg) were 0.23 kg heavier than twins (3.07${\pm}$0.05 kg), in Mutton Merino lambs born as singles (3.99${\pm}$0.08 kg) were 0.07 kg heavier than twins (3.92${\pm}$0.08 kg) and in Dorper lambs born as singles (4.41${\pm}$0.04 kg) were 0.02 kg heavier than twins (4.39${\pm}$0.04 kg). On average males were heavier than females (p<0.05) weighing (3.32${\pm}$0.04 kg vs. 3.05${\pm}$0.07 kg) in indigenous Sabi, 4.73${\pm}$0.03 kg vs. 4.08${\pm}$0.05 in Dorper and 4.26${\pm}$0.07 kg vs. 3.66${\pm}$0.09 kg in Mutton Merino sheep. Two way factor interactions of sire*year, year*sex and sex*birth status had significant effects (p<0.05) on birth weight in indigenous Sabi, Mutton Merino and Dorper sheep while the effect of year*birth status was non significant on birth weight in Indigenous Sabi sheep. The three way factor interaction of year*sex*birth status had a significant effect (p<0.01) on birth weight in indigenous Sabi and Mutton Merino. Tupping weight fitted as a covariate had significant effects (p<0.001) on birth weight in indigenous Sabi, Mutton Merino and Dorper sheep. The mean weaning weights were 17.94${\pm}$0.31 kg, 18.19${\pm}$0.28 kg and 14.39${\pm}$0.28 kg for Mutton Merino, Dorper and Indigenous Sabi sheep, respectively. Effects of sire and sire*year were non significant on weaning weight in Dorper and Mutton Merino while year, sex and sex*year interaction had significant effects (p<0.001) on weaning weight. On average males were heavier than females (p<0.001) at weaning. The respective weaning weights were 18.05${\pm}$0.46 kg, 18.68${\pm}$0.19 kg, 14.14${\pm}$0.15 kg for males and 16.64${\pm}$0.60 kg, 16.41${\pm}$0.31 kg, 12.64${\pm}$0.32 kg for females in Mutton Merino, Dorper and Indigenous Sabi sheep. Lambs born as singles were significantly heavier at weaning than twins, 0.05 kg, 0.06 kg and 0.78 kg for Mutton Merino, Dorper and Indigenous Sabi sheep, respectively. Effect of tupping weight was highly significant on weaning weight. The three way factor interaction year*sex*birth status had a significant effect (p<0.01) on weaning weight. Correction for environmental effects is necessary to increase accuracy of direct selection for birth weight and weaning weight.

A Study on Weight Control and Dietary Life among Middle School Students: Nutrition Knowledge, Dietary Behavior and Food Frequency (남녀 중학생들의 체중조절과 식생활 비교 연구: 영양지식, 식생활 태도 및 식품 섭취 빈도)

  • Kang, Hee-Won;Lee, Sang-Sun
    • Journal of Nutrition and Health
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    • v.39 no.8
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    • pp.817-831
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    • 2006
  • The purpose of this study is to compare the nutrition knowledge, dietary behavior and food frequency according to the gender and weight control attempt among middle school students in Seoul and Gyunggi-do, Korea. The subjects were divided into three groups, one with experience in attempting to lose their weight, one with experience in attempting to gain their weight and one with no experience in attempting to control their weight. Nutrition knowledge level of girls was higher than that of boys (p<0.01), but there were no significant difference according to weight control attempt. As for the scores of dietary behavior, there were no significant differences by gender but according to weight control attempt, the scores of dietary behavior of the weight loss attempt group were higher than those of the other attempt groups (p<0.01). In the food intake frequency, there were significant differences according to weight control attempt (p<0.05, p<0.01).

A Study on the Characteristics of Lower Extremity Weight Bearing in Patients with Low Back Pain (요통환자의 기립시 하지체중 지지특성에 관한 연구)

  • Yoon, Hong-Il;Bae, Soo-Chan
    • The Journal of Korean Academy of Orthopedic Manual Physical Therapy
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    • v.5 no.1
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    • pp.59-74
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    • 1999
  • 66 patients with low back pain were evaluated to determine if any differences of weight bearing were existed between the same side leg that feel pain(pain side leg) and that without pain (non-pain side leg) of lower extremities while weight bearing. 66 patients were divided into 3 groups according to pain pattern such as low back pain only(26), radiating pain only(21) and low back pain with radiating pain (19). Control group were 28 normal persons. The weight bearing was rated by Limloader(Model LLD-2000, Ver 1.2) and analyzed by paired and independent t-test, one-way ANOVA, Dunan's analaysis and Pearson's correlation analysis. The results of this study were as follows : 1. In patient group, significant difference of weight bearing rating between pain side leg and non-pain side leg was found. There was borne significantly less weight bearing rating(14.68%) on the pain side leg(P<.001). 2. In normal group, there was no significant difference between left and right leg(P>.05). But there was significant difference(3.21%) in absolute difference of both leg(p<.001). 3. The difference of 1 and 2 was 11.47% : more difference of weight bearing rating between both legs in patient group than that of the control group(p<.001). 4. There were significant differences of weight bearing rating in those 3 groups : low back pain group(10.30%), radiating pain group (17.90%) and low back pain with radiating pain group (17.10%) (p<.001). 5. There was significant correlation between pain intensity and difference of weight bearing rating(p<.05). The severer pain intensity. the more difference of weight bearing rating was found. 6. There was no significant correlation between the age, height and duration of symptom, etc(p>.05).

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A Study on Alkali-Treatment of Polyester/silk Union Cloth (폴리에스테르/견 교직물의 알칼리 감량가공에 관한 연구)

  • Lee, Seok-Young;Park, Sung-Woo;Seo, Mal-Yong;Cho, Kyu-Min;Gu, Kang
    • Textile Coloration and Finishing
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    • v.10 no.5
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    • pp.39-47
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    • 1998
  • The purpose of this study was to investigate the effect of alkali treatment on EG solution of polyester/silk union cloth(P/S cloth) . Tensile strength, moisture regain, crease resistance, and reduction ratio of the treated P/S cloth were measured. The results of this study were as follows : 1) The weight loss of P/S cloth treated with EG solution was three times higher than those of P/S cloth treated with $H_2O$. The weight loss of P/S cloth was increased greatly with increasing concentration of NaOH, temperature, and time. Favorable weight reduction of treating condition could be obtained when lower concentration of NaOH was used with longer time. 2) If it was added $H_2O$ on EG solution, weight loss of polyester increased, while those of silk decreased. In addition, decreasing ratio of tensile strength warp direction (polyester) was lower, while those of weft direction(silk) was higher. 3) Moisture regain of P/S cloth treated with EG solution increased with weight loss up to 10% . Crease resistance of P/S cloth was the highest at weight loss of 10~15%.

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A NOTE ON THE q-ANALOGUE OF KIM'S p-ADIC log GAMMA TYPE FUNCTIONS ASSOCIATED WITH q-EXTENSION OF GENOCCHI AND EULER NUMBERS WITH WEIGHT α

  • Araci, Serkan;Acikgoz, Mehmet;Park, Kyoung Ho
    • Bulletin of the Korean Mathematical Society
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    • v.50 no.2
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    • pp.583-588
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    • 2013
  • In this paper, we introduce the $q$-analogue of $p$-adic log gamma functions with weight alpha. Moreover, we give a relationship between weighted $p$-adic $q$-log gamma functions and $q$-extension of Genocchi and Euler numbers with weight alpha.

Regularity for Very Weak Solutions of A-Harmonic Equation with Weight

  • Gao, Hong-Ya;Zhang, Yu;Chu, Yu-Ming
    • Kyungpook Mathematical Journal
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    • v.49 no.2
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    • pp.195-202
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    • 2009
  • This paper deals with very weak solutions of the A-harmonic equation $divA(x,{\nabla}u)$ = 0 (*) with the operator $A:{\Omega}{\times}R^n{\rightarrow}R^n$ satisfies some coercivity and controllable growth conditions with Muckenhoupt weight. By using the Hodge decomposition with weight, a regularity property is proved: There exists an integrable exponent $r_1=r_1({\lambda},n,p)$ < p, such that every very weak solution $u{\in}W_{loc}^{1,r}({\Omega},{\omega})$ with $r_1$ < r < p belongs to $W_{loc}^{1,p}({\Omega},{\omega})$. That is, u is a weak solution to (*) in the usual sense.

Comparative study on body shape satisfaction and body weight control between Korean and Chinese female high school students

  • Ro, Yoo-Na;Hyun, Wha-Jin
    • Nutrition Research and Practice
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    • v.6 no.4
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    • pp.334-339
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    • 2012
  • This study was conducted to compare body shape satisfaction, body image perception, weight control status, and dietary habits of Korean and Chinese female high school students in order to provide information for proper body image perception of adolescents. 221 students in Yongin, a city in Korea, and 227 students in Weihai, a city in China, were surveyed using questionnaires. Body shape satisfaction was significantly higher in Chinese students (P < 0.001) compared to Korean students. 76.2% of Korean students and 72.7% of Chinese students wanted a thinner body shape than their present body shapes. Experiences of weight control, laxative or diuretics uses, eating during weight control, and vomiting after eating were significantly higher in Korean students (P < 0.05-P < 0.001) compared to Chinese students. The score for dietary habits was significantly higher in Chinese students (P < 0.001) compared to Korean students, suggesting a more desirable dietary habit among Chinese students. Students of both countries showed a significantly positive correlation between body shape satisfaction and dietary habits, suggesting that as body shape satisfaction increases, dietary habits become more desirable. In conclusion, Korean female students showed a more distorted body image perception and had more poor dietary habits than Chinese students. Nutritional education for the establishment of normal body weight, proper body image perception, and healthy dietary habits are needed.

Effect of Enzyme Supplementation on the Performance of Growing-Finishing Pigs Fed Barley-Based Diets Supplemented with Soybean Mealor Canola Meal

  • Thacker, P.A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.7
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    • pp.1008-1013
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    • 2001
  • This experiment was conducted to determine the effects of enzyme supplementation on the performance of 80 growing-finishing pigs (26.2 kg) fed diets containing either soybean or canola meal. Barley-based diets formulated using either soybean meal or canola meal were fed with or without enzyme (Allzyme Vegpro, Alltech Biotechnology Centre). Eight castrates and twelve gilts were fed each diet. Digestibility of dry matter, crude protein and gross energy was 8.0 (p=0.0001), 7.9 (p=0.0005) and 7.9 (p=0.0003) percent lower for pigs fed diets containing canola meal compared with soybean meal. Enzyme supplementation had no effect on nutrient digestibility (p>0.05). There was a significant interaction between protein source and enzyme for all three nutrients. Over the entire experimental period (26.2 to 77.9 kg), pigs fed canola meal consumed 9.4% less feed (p=0.001), gained weight 20.4% slower (p=0.001) and had a 12.9% poorer feed conversion (p=0.001) than pigs fed soybean meal. Weight gain, feed intake and feed conversion were unaffected by enzyme addition (p>0.05). Castrates gained weight 11.4% faster (p=0.001), consumed 9.3% more feed (p=0.001) and had a 2.6% better feed conversion (p=0.026) than gilts. There was a significant interaction between protein source and sex of pig for feed conversion. Pigs fed diets based on canola meal had a significantly lower carcass value index (p=0.01), lower lean yield (p=0.007) and lower lean depth over the loin (p=0.001) than pigs fed diets based on soybean meal. Enzyme addition significantly increased lean depth over the loin (p=0.01). There was a significant interaction between protein source and enzyme for carcass value index (p=0.04), estimated lean yield (p=0.05) and fat depth over the loin (p=0.05). These results confirm previous studies which have demonstrated poorer pig performance when canola meal completely replaces soybean meal in diets fed to growing-finishing pigs. In addition, the results provide little justification for the inclusion of the Vegpro enzyme in diets fed to pigs of this weight range.

Gender Difference in the Effects of Gonadectomy and Hypercholesterol Diet on Plasma and Liver Cholesterol and Triglyceride bevels, Platelet Aggregation and Liver Tissue in Sprague Dawley Rats (성소의 제거와 고콜레스테롤 식이 급여가 흰쥐의 혈장과 간의 콜레스테롤, 중성지방 수준과 혈소판 응집성 및 간 조직에 미치는 영향에 있어서 암.수의 차이)

  • 오인숙;강정애;강정숙
    • Journal of Nutrition and Health
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    • v.35 no.1
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    • pp.12-23
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    • 2002
  • Gender differences in the effects of gonadectomy and high cholesterol diet on body weight and body cholesterol were investigated by using Sprague Dawley rats. Body weight, plasma and liver levels of cholesterol and triglyceride and platelet aggregation were examined in ovariectomized(OVX) or orchidectomized(ODX) rats with their intacts after feeding diet with or without 0.5% cholesterol. Body weight was significantly increased(p < 0.01) in OVX rats and significantly decreased(p < 0.01) in ODX rats compared to their respective intact rats, and cholesterol diet significantly(p < 0.05) decreased body weight in gonadectomized rats. Liver lobes from rats fed cholesterol diet were opaque and larger than those from rats find control dict, resulting in a significant increase(p < 0.01) in LW/BW ratio. Plasma and liver levels of total cholesterol were significantly increased (p < 0.01) in female rats regardless ovariectomy when find 0.5% cholesterol diet, but those levels in male rats were increased only when they were orchidectomized(p < 0.0l). Plasma HDL-cholesterol was significantly decreased(p < 0.05) in both sexes when find cholesterol diet. HDL-cholesterol were higher in female than male rats regardless treatments(p < 0.05). Liver triglyceride was significantly increased(p < 0.05) in both sexes when find cholesterol dict. Plasma level of triglyceride was not different among groups except significant decrease(p < 0.05) in cholesterol find ODX rats. Maximum platelet aggregation in female rats was significantly lower(p < 0.05) than male, but ovariectomy and cholesterol diet caused an increase te the level of male rats. Microscopic examination showed cholesterol diet caused a lipid accumulation in liver. Results indicate that intact female rats have higher response to hypercholestcrolemic diet than intact male rats and orchidectomy causes male rats more responsive to hypercholesterolemic diet. However, ovariectomy causes an increase female food efficiency ratio to the level of male rats, significantly increasing body weight.