• 한국의류학회지
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• 제23권3호
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• pp.353-360
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• 1999

An investigation made of the variations of angle of bias on the top of the sleeve cap curve line and calculated easing contraction ratio by capheights(A ; a,h$\times$5,/6) B: A, H/4 +4cm C:A.H/3 D: A.H/ 4+3cm E:AH/4+2cm, F: A,H/4+1cm, G: A,H/4, H:A,H/6, I:A,H/8) and the efects of easing contraction on the cap curve lines of sleeve A, D, G by easing stitch density with the gathering foot: sewing condition-lockstitch industrial machine stitch density(N1.0 ; 38stitches/3cm N1.5: 26stitches/3cm, N2.0 ; 19stitches/3cm, N2.5 ; 14stitches/ 3cm) The results obtained were as follows; 1) The variations of the angle of bias on the top of the sleeve cap curve line by cap heights can be done according to the angle balance (front; $\alpha$-$\beta$ back ; $\alpha$'- $\beta$') between the angle (front ;$\alpha$, $\beta$, back ; $\alpha$'- $\beta$') of bias of the two base-lines. 2) The higher cap height the more higher the calculated easing contraction ratio. 3) The lower the stitch density the higher easing contraction ratio. 4) The effects of easing contraction was that sleeve G was more than sleeve A, D.

• Tuberculosis and Respiratory Diseases
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• 제55권1호
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• pp.21-30
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• 2003

서 론 : MUC genes의 증가와 배상세포의 증식 기전에 성장인자(growth factor)인 상피세포 성장인자 및 수용체(epidermal growth factor receptor; EGFR)가 배상세포의 증식이나 이형성에 관여한다. EGFR의 ligands 중의 한 종류인 heparin binding EGF(HB-EGF)는 세포막에 존재하는 pro-heparin binding EGF(pro-HB-EGF)로부터 유리된다. HB-EGF의 유리는 G-protein과 연관이 있다. 따라서, 본 연구는 그람 음성세균의 lipopolysaccande(LPS)에 의한 기도 점액 과생성의 기전을 밝히고, 기도점액 과분비에서 EGFR과 G-protein의 연관성을 밝혀 기도 점액 과분비 기전을 밝히고자 한다. 연구방법 : NCI-H292 세포배양에서 LPS단독 투여 또는TGF-${\alpha}$와 병합 투여한 후 MUC5AC의 당단백질을 ELISA법으로 측정하였다. LPS에 의한 MUC5AC 당단백질의 생성 기전을 밝히기 위해서 heterotrimeric G-protein 억제제인 mastoparan을 투여하고 TNF-${\alpha}$와 MUC5AC를 ELISA법으로 각각 측정하였다. MUC5AC의 생성에서 G-protein과 EGFR의 연관성을 확인하기 위하여 EGFR이 항상 발현되어 있고 MUC5AC를 분비할 수 있는 NCI-H292 세포에 G-protein 자극제인 mastoparan-7로 자극한 후 MUC5AC의 생성을 측정하였다. G-protein이 활성화하여 metalloproteinase가 세포막에 있는 HB-EGF를 유리하여 EGFR이 활성화하여 MUC5AC가 생성여부를 확인하기 위하여 ADAM10으로 NCI-H292세포에 자극하여 MUC5AC의 생성을 측정하였다. MUC5AC 생성이 EGFR과 연관성을 확인하기 위하여 특이 EGFR tyrosine kinase 억제제인 AG1478과 중화 polyclonal EGF 항체를 전처치 후 MUC5AC를 측정하였다. 결 과 : LPS의 자극에 의한 MUC5AC의 생성은 LPS 농도에 유의하게 증가 되지 않았으나, EGFR의 ligand인 TGF-${\alpha}$를 동시 투여한 경우는 LPS의 농도에 비례하여 유의하게 증가하였다. LPS의 자극은 TNF-${\alpha}$의 생성을 유의하게 증가시켰으며, G-protein 억제제인 mastoparan을 전처치한 경우는 TNF-${\alpha}$가 유의하게 감소 되었다. LPS 자극 전에 TNF-${\alpha}$ antibody, AG1478 또는 mastoparan을 전처치한 경우는 MUC5AC의 생성이 유의하게 억제되었다. MUC5AC의 생생에서 G-protein과 EGFR의 연관성에 대한 실험에서 MUC5AC의 생성이 mastoparan-7의 농도에 따라 유의하게 증가되었으며, EGF의 중화항체를 사용한 경우는 MUC5AC의 생성이 감소되었다. 또한 Matrix metalloproteinase인 ADAM10의 농도에 비례하여 MUC5AC의 생성을 증가시켰다. 결 론 : LPS에 의한 MUC5AC의 분비는 LPS가 TNF-${\alpha}$를 생성시키고, TNF-${\alpha}$가 EGFR의 발현을 유도하여 MUC5AC가 분비되었다. 또한 MUC5AC의 생성에 있어서 G-protein의 활성은 matrix metalloproteinase에 의하여 EGFR의 ligand 인 HB-EGF가 유리되어 EGFR의 transacti vation으로 MUC5AC가 생성되는 것으로 사료된다.

• Applied Biological Chemistry
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• 제49권3호
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• pp.180-185
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• 2006

본 연구는 Debaryomyces sp.가 생산하는 ${\alpha}-galactosidase$의 mannobiose-sepharose 담체합성법에 의한 affinity column chromatography를 수행하여 효소정제, 효소 화학적 성질 및 galactosyl mannooligosacchrides에 대한 기질특이성 규명을 주요 목적으로 하였다. 배양시간별 활성과 pH의 변화에서 배양시간 40시간부터 활성이 증가하고 있으며, 70시간에서 25.8unit/ml의 최대활성을 나타내고 있으며, 배양초기 pH 6.0에서 시작되어 배양말기에는 pH 8을 나타내는 pH의 변화를 보였다. 최적 pH는 4.0, 최적온도는 $60^{\circ}C$이며 $pH\;3{\sim}4.5$에서 100%의 잔존활성을 나타낸 반면 pH 8.0에서는 20%로 급격히 감소하였고. 온도안정성에서 $30{\sim}50^{\circ}C$에서는 100%의 잔존활성을 나타내었으나 $70^{\circ}C$ 이상에서는 20% 이하의 잔존활성을 나타내었다. $Hg^{2+}$에 의해서 54%, $Ag^{2+}$에 의해서는 85%로 저해되었으며, 그 이외의 이온에 대해서는 큰 영향을 받지 않았다. Debaryomyces sp.유래 ${\alpha}-galactosidase$는 24시간 반응 후 melibiose, $Gal^3Man_3$를 완전 가수분해 하여 각각 galactose와 glucose, galactose와 mannotriose로 분해되었으나 $Gal^3Man_4$에 대해서는 12시간 반응시켜도 기질로부터 galactose 유리가 불가능함이 확인되었다.

• Bulletin of the Korean Chemical Society
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• 제27권1호
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• pp.93-98
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• 2006

Two new compounds 1'-(stigmast-11,20(21),25-trien 3$\alpha$,9$\alpha$-diolyl)-3'-(pimara-11,15-dien-3$\alpha$-olyl) glycerol (1) and stigmast-5-en-3$\alpha$,26-diol (2) along with known fatty acids n-hexacosanoic acid (3) and hexadecanoic acid (4), have been isolated from the methanol extract of rice hulls of Oryza sativa. The structures of the compounds were elucidated using 1D and 2D NMR spectral methods, viz; $^1H, ^{13}C, ^{13}C$-DEPT, $^1H-^1H$ COSY, $^1H-^{13}C$ HETCOR, HSQC and HMBC aided by IR, EIMS, FABMS and HRFABMS. Compound (1) showed inhibition to radish germination, growth of shoot and root length.

• The Korean Journal of Ceramics
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• 제2권1호
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• pp.39-42
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• 1996

${\alpha}-SiC$ powder with or without the addition of 0.1 wt% of large ${\alpha}-SiC$ partices(seeds) was pressureless-sintered at $1950^{\circ}C$ for 0.5, 2, and 4 h using $Y_3Al_5 O_{12}$ (yttrium aluminum garnet, YAG) as a sintering aid. The materials without seeds had an equiaxed grain struture. In contrast, the materials with seeds sintered for 2 and 4 h had a duplex microstructure with large elongated grains and amall equiaxed grains. Addition of large ${\alpha}-SiC$ seeds into ${\alpha}-SiC$ accelerated the grain growth of some ${\alpha}-SiC$ grains during sintering and resulted in the increased fracture toughness of the sintered materials. The fracture toughnesses of materials with or without seeds sintered for 4 h were 6.6 and $5.2 MPa \;m^{12}$, respectively.

• International Journal of Industrial Entomology and Biomaterials
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• 제8권2호
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• pp.189-194
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• 2004

Amylase isozyme based three multivoltine viz., N+p, Np, N+ $p^{cho}$ and two bivoltine-D6+p, D6p syngenic lines (Syn. L) were developed from germplasm (GP) stocks Nistari (N) and D6 respectively. haemolymph isozyme pattern at pH 7.0 and 8.5 depicted a total 11 number (Am $y_{1 to 6}$ at pH 7.0 and Am $y^{l to 5}$ at pH 8.5) of native proteins (NP) of various sizes are amylase isozyme expressers. Among eleven NPs, two NPs of 770 kDa (Am $y^{6}$ at pH 7.0) and 376 kDa (Am $y^3$ at pH 8.5) are $\alpha$-amylase expressers and remaining NPs of 370, 364, 350, 329 and 274 kDa at pH 7.0 and 206, 292, 416, 725 kDa at pH 8.5 are $\beta$-amylase expressers. Accordingly, digestive juice amylase isozyme pattern at aforesaid pH also depicted a total number of 10 NPs (Am $y^{1 to 5}$) at each pH 7.0 and 8.5 are amylase expressers of which NP of 387 kDa (Am $y^4$ at pH 7.0) and 780 kDa (Am $y^{5}$ at pH 8.5) are a-amylase expresser. Remaining NPs of 338,297 ＆ 216 kDa at pH 7.0 and 370, 341, 329 ＆302 kDa at pH 8.5 are $\beta$-amylase expresser. Recurrent backcross lines (RBL) viz., N+pRBL and NpRBL were developed through introgression of high shell weight character (a multigenic trait) to be used further for congenic line (Con. L) development and to understand any association with introgressed character. Isozyme pattern in haemolymph of RBLs depicted only one $\alpha$-amylase of 770 kDa at pH 7.0 and 376 kDa at pH 8.0 with three and four respective $\beta$-amylase bands but in bivoltine lines numbers of $\beta$-amylase bands vary between 1 to 2 at aforesaid pH. Variability was also observed in digestive juice of multivolitine and its RBLs but bivoltine lines express null activity at both pH except appearance of one very week $\alpha$-amylase band D6+p at pH 8.5. Overall study suggests that not a single NP at both pH is common for expression of any band of amylase isozyme i.e., a totally different set of proteins are the amylase isozyme expresser at specific pH and no molecular factor of amylase is associated in developed RBLs which showed improvement on survival, single cocoon shell weight (SCSW) and single filament length over receptor parents.s.s.s.

• 천문학회보
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• 제44권1호
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• pp.73.5-73.5
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• 2019

Studying the amount and kinematics of circumand intergalactic medium (CGM and IGM) is key to understanding the role of feedback and environment (cold streams and galactic winds) in the evolution of galaxies. In particular, $Ly{\alpha}$ emission line has been utilized to investigate the density structure and kinematics of the (most abundant) H I gas in the CGM and IGM around galaxies. Therefore, modeling $Ly{\alpha}$ radiative transfer through multiphase interstellar medium (ISM), CGM and IGM is crucial in understanding the galaxy evolution. As discussed in Kakiichi & Dijkstra (2018), most $Ly{\alpha}$ RT effects would occur on interstellar scales. This is because the main source of $Ly{\alpha}$ photons would be H II regions, which are in most cases, if not all, surrounded by "cold" photo-dissociation regions. However, most $Ly{\alpha}$ RT studies have been performed in the CGM and IGM environments with T ~ 10,000K. In this talk, we present how the $Ly{\alpha}$ RT effect in the cold ISM with T ~ 100 K regulates the $Ly{\alpha}$ spectral properties.

• 한국식품영양과학회지
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• 제26권5호
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• pp.844-850
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• 1997

$\alpha$-Galactosidase was partially purified from the culture filtrate of pichia guilliermondii by Mannobiose-Sepharose affinity column chromatography. The galactosidase exhibited maximum activity at pH 4.5 and 4$0^{\circ}C$, and was stable in the pH and temperature ranges of 4 to 5.5 and 30 to 6$0^{\circ}C$, respectively. The enzyme was inhibited by $Hg^{2+}$ and $Ag^{2+}$. The enzyme activity was ot affected considerably by treatment with other metal compounds. The enzyme hydrolyzed melibiose to galactose and glucose, raffinose to galactose and sucrose, and $Gal^{3}Man_{3}(6^{3}-$\alpha$-galactosyl-1,4-mannotriose)$ to galactose and mannotriose. On the contrary, it could not hydrolyze $Gal^{3}Man_{4}(6^{3}-galactosyl-1,4-$\alpha$-mannotetraose)$.

• 대한화학회지
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• 제29권4호
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• pp.397-405
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• 1985

금속킬레이트제로써 잘 알려진 몇가지 oxime 화합물들의 Amberlite XAD 수지들에 대한 흡착성을 분포계수값을 측정함으로써 상호 비교해 본 결과 킬레이트제로서는 분포계수값이 비교적 큰 salicylaldoxime (SAO)과 ${\alpha}$-benzoinoxime(${\alpha}$-BzO) 그리고 수지로서는 XAD-4 수지가 적합함을 알았다. SAO과 ${\alpha}$-BzO를 XAD-4 수지에 각각 침윤시킨 SAO-XAD-4 및 ${\alpha}$-BzO-XAD-4 침윤수지의 그 특성을 조사하였다. SAO와 ${\alpha}$-BzO의 XAD-4 수지에 대한 최적 흡착조건은 30% 메탄올, pH 1∼8(SAO) 및 pH 1~9 (${\alpha}$-BzO)였다. SAO 및 ${\alpha}$-BzO의 XAD-4 수지에 대한 흡착은 온도가 증가함에 따라 감소하였으며, 그 흡착메카니즘은 흡착엔탈피(-${\Delta}$H)를 구해본 결과 4.99 ~ 6.66 (Kcal/mol)인 것으로 보아 쌍극자-쌍극자 인력에 상응하는 분자 흡착임을 알 수 있었다. 한편 금속이온을 흡착시키는 매질용액과 흡착된 금속이온을 회수하는 염산수용액에서 침윤수지의 안정성을 조사한 결과, 전자는 pH 5∼10에서, 후자는 0.1~5M 염산수용액에서 비교적 큰 안정성을 보였다. 두 침윤수지에 의한 Mn(II), Co(II), Ni(II), Zn(II)등의 금속이온의 흡착몰비는 대략 1;2(금속이온:킬레이트제)이었으며 흡착된 금속이온은 3M-HCl 수용액 또는 3M-HCl/50%-MeOH 용리액으로 정량적으로 회수가 가능하였다.

• 한국의류학회지
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• 제17권3호
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• pp.380-390
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• 1993

Effect of interfacial electrical conditions on adhesion of ${\alpha}-Fe_2O_3$ particles to PET fabric and the removal of ${\alpha}-Fe_2O_3$ particles from PET fabric, were investigated as functions of pH, electrolyte and ionic strength. The ${\zeta}$ potential of PET fiber and ${\alpha}-Fe_2O_3$ particles in the electrolyte solution were measured by streaming potential and microelectrophoresis methods respectively. The potential energy of interaction between ${\alpha}-Fe_2O_3$ particles and PET fabric were calculated by using the heterocoagulation theory for a sphere-plate model. The negative ${\zeta}$ potential of ${\alpha}-Fe_2O_3$ particle and PET fiber increased with pH, and then decreased certain pH and isoelectric points of ${\alpha}-Fe_2O_3$ particles and PET fiber were pH 6.5 and pH 3.5, respectively. The negative ${\zeta}$ potential of ${\alpha}-Fe_2O_3$ particle and PET fiber affected by electrolytes, were relatively high with polyanion electrolytes in solutions and were low with neutral salts. However, at surfactant solution, ${\zeta}$ potential was levelled off. The influence of the ionic strength on the ${\zeta}$ potential of ${\alpha}-Fe_2O_3$ particle was small but the negative ${\zeta}$ potential of PET fiber increased with the ionic strength. In the presence of anionic surfactant, the ${\zeta}$ potential of ${\alpha}-Fe_2O_3$ particle and PET fiber increased regardless of solution conditions. The interaction energy between ${\alpha}-Fe_2O_3$ particle and PET fabric increased with pH. The interaction energy was relatively high with polyanion electrolytes in solution, and the influence of ionic strength on the interaction energy was small, and the effective thickness of electrical double layer increased with decreasing the ionic strength.