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S$_H$2 Reaction on Silicon-Carbon Bond in the Photoreactions of 2, 3-Benzo-1, 1-diphenyl(or dimethyl)-1-sila-2-cyclobutene with Carbonyl Compounds

  • Kang, Kyung-Tai;Okazaki, Renji;Inamoto, Naoki
    • Bulletin of the Korean Chemical Society
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    • v.5 no.1
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    • pp.32-37
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    • 1984
  • The photoreaction of 2, 3-benzo-1, 1-diphenyl (or dimethyl)-1-sila-2-cyclobutene (9 or 10) with an aldehyde or ketone results in 1:1 cycloadduct of [4 + 2] type. In the reactions of 2, 3-benzo-1, 1-dimethyl-1-sila-2-cyclobutene (10) with acetone and butanone, another 1:1 adducts (13) were also formed, respectively. The following facts indicate that the formation of adduct involves an attack of a triplet carbonyl compound on the silicon of the benzosilacyclobutene, an $S_H2$ process. (1) Even when the reaction of 9 with acetophenone was carried out under conditions such that more than 99% of incident light was absorbed only by acetophenone using the filter solution of aq. cupric sulfate, the same adduct was still formed. (2) When the reaction of 9 with acetone was carried out under oxygen atmosphere, only trace amount of adduct was formed.

Cis-Trans Isomerization of Dimeric $[Me_2Al(μ-NH^tBu)]_2$

  • Park, Joon T.;오원태;김윤수
    • Bulletin of the Korean Chemical Society
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    • v.17 no.12
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    • pp.1147-1149
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    • 1996
  • The trans (2a)-cis (2b) isomerization of [Me2Al(μ-NHtBu)]2 (2) has been studied by 1H NMR spectroscopy. The equilibrium has been observed to follow reversible first order kinetics with ΔH0=2.22±0.07 kJmol-1 and ΔS0=2.85±0.07 JK-1mol-1. The activation parameters for the conversion 2a→2b are ΔH1=49.7±2.3 kJmol-1 and ΔS1=-126.3±0.2 JK-1mo1-1 and for the reverse reaction 2b→2a are ΔH-1=47.5±2.3 kJmol-1 and ΔS-1=-129.1±0.5 JK-1mol-1. The isomerization is markedly accelerated in the presence of Lewis bases. A crossover experiment indicates that the isomer interconversion is a unimolecular process. The large negative entropies of activation suggest either the existence of a sterically congested intermediate or the participation of solvent in the isomerization process.

Recognition of the Importance of Imidazolidinone Motif for Cytotoxicity of 4-Phenyl-1-arylsulfonylimidazolidinones Using Thiadiazolidine-1, 1-Dioxide Analogs

  • Kim, Il-Whan;Jung, Sang-Hun
    • Archives of Pharmacal Research
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    • v.25 no.4
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    • pp.421-427
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    • 2002
  • For probing the importance of planarity of imidazolidinone motif of 4-phenyl-1-(N-acylindoline-5-sulfonyl)imidazolidinones 1 for their cytotoxicity, 4-phenyl-1-(N-acylindoline-5-sulfonyl)[1,2,5]thiadiazolidine-1,1-dioxides 2 were prepared and their cytotoxicity were measured against human lung carcinoma (A549), human colon carcinoma (COLO205), human ovarian cancer (SK-OV-3), human leukemic cancer (K562), and murine colon adenocarcinoma (Colon26) cell lines in vitro. Although only carbonyl moiety of imidazolidinone ring was replaced with sulfonyl group, compounds 2 do not show any activity against all five cancer cell lines unlike 1. Therefore the planarity of imidazolidinone ring of 1 should be an important factor for their cytotoxic activity.

Synthesis and Magnetic Relaxation Properties of Paramagnetic Gd-complexes of New DTPA-bis-amides. The X-ray Crystal Structure of [Gd(L)(H2O)]·3H2O (L = DTPA-bis(4-carboxylicphenyl)amide)

  • Dutta, Sujit;Kim, Suk-Kyung;Lee, Eun-Jung;Kim, Tae-Jeong;Kang, Duk-Sik;Chang, Yong-min;Kang, Sang-Ook;Han, Won-Sik
    • Bulletin of the Korean Chemical Society
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    • v.27 no.7
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    • pp.1038-1042
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    • 2006
  • A new type of DTPA-bis-amides (L1-L4) and their Gd(III)-complexes of the type $[Gd(L)(H_2O)]{\cdot}nH_2O$ (5: L1; 6: L2; 7: L3; 8: L4) have been prepared and characterized by analytical and spectroscopic techniques. The X-ray crystal structure of 8 has been determined for structural confirmation. The coordination geometry adopts a tricapped trigonal prism geometry with L4 acting as a chelate octadentate and a water molecule in the coordination sphere. Crystals are monoclinic, $P2_1$, a = 14.468(3), b = 19.235(4), c = 13.527(2) $\AA$ $\beta$ = $107.245(3)^{\circ}$, V = 3595.2(11) $\AA^3$, Z = 4, $D_{calc}$ = 1.570. Significant increases in relaxivities are observed with 6 and 7 as compared with that of $Omniscan^{(R)}$, a commercial MR agent: R1 = 12.46 $mM^{-1}\;s^{-1}$, R2 = 8.76 $mM^{-1}\;s^{-1}$ for 6; R1 = 12.77 nm-1 s-1, R2 = 7.60 mM-1 s-1 for 7; R1 = 4.9 $mM^{-1}\;s^{-1}$, R2 = 4.8 $mm^{-1}\;s^{-1}$ for $Omniscan^{(R)}$. In the case of 5, however, both R1 and R2 are found to be lower to show 2.09 $mM^{-1}\;s^{-1}$, and 1.82 $mM^{-1}\;s^{-1}$, respectively.

Study on Sensory Papillae of Metagonimus yokogawai Cercaria (Metagonimus yokogawai 세르카리아의 감각유두에 관한 연구)

  • 김재진;민득영소진탁
    • Parasites, Hosts and Diseases
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    • v.22 no.1
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    • pp.11-20
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    • 1984
  • A number of studies on the papillae of cercariae of trematodes reported that the papillar patterns (or chaetotaxy) of cercariae might be an excellent method to attain better understanding of the digenetic trematodes (Richard, 1971 ; Short and Cartrett, 1973; Bayssade-Dufour, 1979) . The present study was aimed to determine the number, distribution pattern and structure of the sensory papillae of Metagonimus yokogawai cercariae, and to elucidate the chaetotaxy of this digenetic trematode. M. yokogawai cercariae were pipetted from a vial in which infected snails (Semisulcospira libertina) had been kept for 3 hours. The snails were collected from an endemic area of M. yokogawai, Boseong river in west-southern part of Korea. Observations of papillae were based on light microscopy of those stained with silver nitrate, and on scanning electron microscopy The results are summarized as follows: 1, All papillae observed were uniciliated. 2. Cilia in anterior tip were shorter than the others in other portions. 3. The body papillae were arranged in essentially symmetrical patterns, Total number of the papillae was 126(63 pairs) in average; anterior tip 40(20 pairs), ventral 20(10 pairs), lateral 42(21 pairs), and caudal 8(4 pairs). 4. The chaetotany of M. yokogawai cercaria was: Ci cycle ($3+3C_{I}V,{\;}2+2C_{I}L,{\;}2+3C_{I}D),{\;}C_{II}{\;}cycle(2C_{II}V,{\;}1C_{II}L,{\;}2C_{II}D),{\;}C_{lll}{\;}cycle{\;}(1+lC_{III}V,{\;}1C_{IlI}L),{\;}C_{IV}{\;}cycle{\;}(1C_{IV}V,{\;}IC_{lV}L){\;}in{\;}cephalic{\;}region:{\;}A_I(1A_{IV}V,{\;}1+2A_{I}L,{\;}1A_{I}D),{\;}A_{II}(1A_{II}V,{\;}1+3A_{II}L,{\;}1A_{II}D),{\;}A_{III}(1A_{III}V,{\;}1+1A_{III}L,{\;}1A_{III}D){\;}and{\;}A_{IV}(1A_{IV}V,{\;}2A_{IV}L)$ in antacetabular region: $1M_{I}V{\;}and{\;}2M_{I}L$ in median: $1+1P_{I}L,{\;}1P_{II}L,{\;}1P_{II}D,{\;}1P_{III}L,{\;}1P_{IV}L{\;}and{\;}1P_{IV}D$ in postacetabular region: 2-2-2-2 in caudal region.

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Simultaneous Determination of Prostaglandin E1 and Prostaglandin E1 Ethyl Ester in Hairless Mouse Skin Homogenate by High-Performance Liquid Chromatography

  • Choi, Han-Gon;Kim, Ji-Hyun;Li, Dong-Xun;Piao, Ming-Guan;Kwon, Tae-Hyub;Woo, Jong-Soo;Choi, Young-Wook;Yoo, Bang-Kyu;Yong, Chul-Soon
    • Journal of Pharmaceutical Investigation
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    • v.35 no.5
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    • pp.375-381
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    • 2005
  • A rapid and specific high-performance liquid chromatographic method was developed and validated for the simultaneous determination of prostaglandin $E_{1}\;(PGE_{1})$ and prostaglandin $E_{1}$ ethyl ester $(PGE_{1}-EE)$ in hairless mouse skin homogenate. The sample treatment procedure involved deproteination and precipitation by acetonitrile. $PGE_{1}$ and $PGE_{1}-EE$ in supernatant were separated in a reversed-phase C18 column without being interfered by other components present in hairless mouse skin homogenate. 9-Anthracenecarboxylic acid was used as an internal standard. The retention times of $PGE_{1}$, 9-anthracenecarboxylic acid and $PGE_{1}-EE$ were, 4.5, 9.5 and 18.0 min, respectively. The assay showed linearity from 1 to $40\;{\mu}g/ml$ for both $PGE_{1}$ and $PGE_{1}-EE$. Precision expressed as RSD ranged from 2.3 to 14.1 % for $PGE_{1}$ and 1.6 to 11.0% for $PGE_{1}-EE$. Accuracy ranged from 100.5 to 119.6 % for $PGE_{1}$ and from 98.0 to 103.7% for $PGE_{1}-EE$. This method was employed successfully to follow the time course of concentrations of $PGE_{1}$ and $PGE_{1}-EE$ in hairless mouse skin homogenate for stability study.

Effect of Ethanol on Aerobic Biodegradation of Benzene, Toluene, and Ethylbenzene by Rhodococcus sp. EH831 (Rhodococcus sp. EH831에 의한 벤젠, 톨루엔 및 에틸벤젠의 호기성 생분해에 미치는 에탄올의 영향)

  • Lee, Seung-Ha;Lee, Eun-Hee;Cho, Kyung-Suk
    • Microbiology and Biotechnology Letters
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    • v.37 no.3
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    • pp.243-247
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    • 2009
  • The usage of ethanol (EtOH)-blended gasoline (gasohol), has been increasing in recent years. EtOH has influence on the distribution and biodegradation of aromatic compounds such as BTEX (benzene (B), toluene (T), ethylbenzene (B), and xylene (X)) that are gasoline compositions. In this study, the effect of EtOH on the aerobic biodegradation of B, T and E was investigated using a BTEX and EtOH-degrading bacterium, Rhodococcus sp. EH831. The degradation rates of B in the conditions of 1:1, 1:4, and 1:0.25 mixtures with EtOH (B:EtOH, mol:mol) were ranged from $3.82{\pm}0.20$ to $5.00{\pm}0.37{\mu}mol{\cdot}g-dry$ cell wight $(DCW)^{-1}{\cdot}h^{-1}$. The degradation rate of T was the fastest in the 1:0.25 mixture ($6.63{\pm}0.06{\mu}mol{\cdot}g-DCW^{-1}{\cdot}h^{-1}$), and it was the lowest in the 1:4 mixture ($4.41{\pm}0.04{\mu}mol{\cdot}DCW^{-1}{\cdot}h^{-1}$). The degradation rates of E were increased with increasing the addition amount of EtOH: The degradation rate of E was the highest in the 1:4 mixture ($1.60{\pm}0.03{\mu}mol{\cdot}g-DCW^{-1}{\cdot}h^{-1}$), and the rates were $1.42{\pm}0.06$, $1.30{\pm}0.01$, and $1.01{\pm}0.30{\mu}mol{\cdot}g-DCW^{-1}{\cdot}h^{-1}$ in the 1:1, 1:0.25, 1.0 mixtures, respectively. In conclusion, the biodegradation of B, T, E by Rhodococcus sp. EH831 was not significantly inhibited by the co-existence of EtOH.

Contrast Sensitivity as a function of spatial frequency by using polarization (편광을 이용한 눈의 공간주파수-대비민감도 함수 측정기구 설계)

  • Kim, Young-Geun
    • Journal of Korean Ophthalmic Optics Society
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    • v.5 no.1
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    • pp.43-48
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    • 2000
  • It was to adjust the luminance of light by the rotation angle of the polarizes and analyzer. The luminance value Lmax, Lmin of Contrast Sensitivity could be obtained from the rotation angle ${\theta}_m$ of the average luminance($L_m$), the rotation angle(${\theta}_{max}$, ${\theta}_{min}$) of the maximum and the minimum's amplitude. $$L_{max}=I(0)e^{-2at}{\cdot}cos^2{\theta}_m(1+C_s^{-1})$$ $$L_{min}=I(0)e^{-2at}{\cdot}cos^2{\theta}_m(1-C_s^{-1})$$ We obtained the rotation angle(${\theta}_{max}$, ${\theta}_{min}$) of the polarizes and analyzer from the rotation angle ${\theta}_m$ of the average luminance($L_m$) and the Contrast Sensitivity($C_s$). $${\theta}_{max}=cos^{-1}[cos{\theta}_m{\cdot}(1+C_s^{-1})^{1/2}]$$ $${\theta}_{min}=cos^{-1}[cos{\theta}_m{\cdot}(1-C_s^{-1})^{1/2}]$$.

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Effect of Substrates on Growth and Yield of Hydroponically Grown Cucumber in Bag Culture (자루재배에서 배지의 종류가 오이의 생육 및 수량에 미치는 영향)

  • 이응호;이재욱;권지선;남윤일;조일환;권영삼
    • Journal of Bio-Environment Control
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    • v.5 no.1
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    • pp.15-22
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    • 1996
  • Cucumbers(Cucumis sativus L. CV. SHARP 1) were cultivated by bag culture for 5 months to investigate the effect of substrates on growth, yield, and mineral contents. The substrates used in the experiment were vermiculite + perlite +peatmoss(1:1:1=V:V:V), perlite+peatmoss(1:1=V:V), perlite+carbonized chaff(7:3=V:V), rockwool, sawdust, and chaff+chaff powder under 10 mesh (7:3=V:V). The rate of moisture content was highest at chaff powder under 10 mesh as 42.5% and was lowest at perlite+carbonized chaff as 31%. Plant height, No. of leaves, fresh and dry weight were increased in vermiculite+perlite+peatmoss(1:1:1=V:V:V). Marketable fruits and yield also showed same aspect with growth. All kinds of mineral contents except K in cucumber plants were higher at vermiculite+perlite+peatmoss(1:1:1=V:V:V), but K content was higher at perlite+ carbonized chaff(7:3=V:V) than those other substrates.

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PEP-1-paraoxonase 1 fusion protein prevents cytokine-induced cell destruction and impaired insulin secretion in rat insulinoma cells

  • Lee, Su Jin;Kang, Hyung Kyung;Choi, Yeon Joo;Eum, Won Sik;Park, Jinseu;Choi, Soo Young;Kwon, Hyeok Yil
    • BMB Reports
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    • v.51 no.10
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    • pp.538-543
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    • 2018
  • Pancreatic beta cell destruction and dysfunction induced by cytokines is a major cause of type 1 diabetes. Paraoxonase 1 (PON1), an arylesterase with antioxidant activity, has been shown to play an important role in preventing the development of diabetes in transgenic mice. However, no studies have examined the anti-diabetic effect of PON1 delivered to beta cells using protein transduction. In this study, we expressed the cell-permeable PON1 fused with PEP-1 protein transduction domain (PEP-1-PON1) to investigate whether transduced PEP-1-PON1 protects beta cells against cytokine-induced cytotoxicity. PEP-1-PON1 was effectively delivered to INS-1 cells and prevented cytokine-induced cell destruction in a dose-dependent manner. Transduced PEP-1-PON1 significantly reduced the levels of reactive oxygen species (ROS) and nitric oxide (NO), DNA fragmentation, and expression of inflammatory mediators, endoplasmic reticulum (ER) stress proteins, and apoptosis-related proteins in cytokine-treated cells. Moreover, transduced PEP-1-PON1 restored the decrease in basal and glucose-stimulated insulin secretion induced by cytokines. These data indicate that PEP-1-PON1 protects beta cells from cytokine-induced cytotoxicity by alleviating oxidative/nitrosative stress, ER stress, and inflammation. Thus, PEP-1-mediated PON1 transduction might be an effective method to reduce the extent of destruction and dysfunction of pancreatic beta cells in autoimmune diabetes.