• 제목/요약/키워드: ${252}^C$f

검색결과 31건 처리시간 0.028초

제주도 고산에서 수용성 에어로졸의 화학적 성분 분석 (Chemical Analysis of Water Soluble Aerosols at Kosan, Cheju lsland)

  • 이호근;박경윤;서명석;장광미;강창희;허철구
    • 한국대기환경학회지
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    • 제11권3호
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    • pp.245-252
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    • 1995
  • Atmospheric aerosols were collected by a High Volume Tape Sampler from March 1992 to December 1993 ar Korean, Cheju, Korea. The water soluble ion concentrations in aerosol were analyzed. The concentrations of cations (N $a^{+}$, $K^{+}$, $Ca^{2+}$, $Mg^{2+}$, N $H_{4}$$^{+}$) were determined by an Inductively Coupled Plazma(ICP) or an Atomic Absorption Spectrometer(AAS), and those of anions (C $l^{[-10]}$ , N $O_{3}$$^{[-10]}$ , S $O_{4}$$^{2-}$) were analayzed by the capillary electrophoresis method. The $Ca^{2+}$, S $O_{4}$$^{2-}$ and N $O_{3}$$^{[-10]}$ concentrations in spring were higher than those in other seasons. The lowest concentrations of these elements were found in summer, largely due to scavenging by frequent rains. Especially the $Ca^{2+}$ concentration on April was three to four times higher than the annual mean concentration. The enrichment factor(E.F.) of each element was calculated. The annual mean E.F. values of the $Ca^{2+}$, $Mg^{2+}$ and C $l^{[-10]}$ in 1992 were the same as those in 1993 except $k^{+}$ and S $O_{4}$$^{2-}$. The correlation formula between all cations and anions for the whole period was Anions = 0.759 * Cations + 0.066.Cations + 0.066.

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Liver Function Analyses and Spleen Histology Assessment Following the Co-administration of Cisplatin and Methanolic Extract of Portulaca Oleracea in Wistar Rats: An Experimental Study

  • Okafor, Izuchukwu Azuka;Nnamah, Uchenna Somtochukwu;Nnaka, Jude
    • Natural Product Sciences
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    • 제26권3호
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    • pp.252-258
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    • 2020
  • This study investigated the biochemical and histological changes associated with the co-administration of cisplatin and methanolic extract of Portulaca oleracea (MEPO) in adult Wistar rats. Twenty-four (24) adult female Wistar rats were randomly divided into six (6) groups (A-F) (n = 4). Group A served as the control group for the experiment and received no treatment. Group B was given a single dose of cisplatin and served as the cisplatin control group. Group C and D received 400 mg/kg and 800 mg/kg of MEPO 6 hours after a single dose cisplatin injection respectively. Group E and F received 400 mg/kg and 800 mg/kg of MEPO 6 hours before cisplatin injection. The cisplatin injection was 2 mL/kg given intraperitoneally for all groups. There was a significant increase in the serum levels of ALT, ALP, AST, total bilirubin, and conjugated bilirubin following cisplatin treatment (p = 0.000, 0.000, 0.039, 0.000, 0.004 respectively) with a consequent reversal due to MEPO administration across all treated groups (p = 0.000, 0.000, 0.000, and 0.000) in a dose-dependent fashion. Cisplatin caused the expansion of the red and white pulp in the spleen which was attenuated by MEPO. MEPO demonstrated a protective effect against cisplatin-induced liver and spleen toxicity.

볏짚분해과정 중에 생산하는 Fomitopsis palustris 균체 외 Xylanase의 분리정제 및 효소특성 (Purification and Characterization of Xylanase from Fomitopsis palustris in Rice Straw Culture)

  • 윤정준;이영민;최두열;김영균;김영숙
    • Journal of the Korean Wood Science and Technology
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    • 제35권6호
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    • pp.159-165
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    • 2007
  • 본 논문에서는 갈색부후균 Fomitopsis palustris가 볏짚을 분해하는 과정 중에 생산하는 xylanase를 확인하여 분리 정제하고, 아미노산 서열분석을 통해 동정하였다. 그리고 동정된 단백질의 효소특성을 조사하였다. 분리 정제된 단백질은 SDS-PAGE분석에서 43kDa의 분자량을 나타내었고, 아미노산 서열분석에서는 Glycoside Hydrolase family 10에 속하는 xylanase와 높은 상동성을 나타내었다. 정제효소의 기질에 대한 $K_m$치는 $31 mg/m{\ell}$, $V_{max}$는 252.3 U/mg, $K_{cat}$$2.3{\times}10^4/min$이고, 최적 pH 범위는 pH 4.0~5.0 최대 활성 온도는 $70^{\circ}C$로 밝혀졌다.

Effects of polysaccharide fractions from phellodendron chinese SCHNEID on tumor progression and immunopontentiation

  • Jun, Kya-I;Lee, Tae-Kyun;Kim, Cheorl-Ho
    • Advances in Traditional Medicine
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    • 제1권1호
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    • pp.37-44
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    • 2000
  • In the previous paper (Kim et al., Glycoconjugate Journal 16, 247-252, 1999), heteropolysaccharides from Korean medicinal plant, Phellodendri cortex (Hwangbek) showed a poten B-Iymphocyte-stimulating activity in a system using polyclonal antibody forming cells in C57BL/6XC3H mice at dosages of 2-10 mg. In a series of biolgical active polysaccharides from natural medicinal plants, the polysaccharide fractions were isolated and purified from Phellodendron chinese SCHNEID, and antitumor activities were examined at dosages of 2, 5 and 10 mg/100 g. F-7 and F-8 showed the highest tumor inhibitory activities (inhibition ratio 96.4 and 98.2% in 2 mg/100 g), and in dose of 5 mg/100 g, the inhibitory ratios were 95.3 and 97.5%, respectively. Furthermore, 10 mg/100 g of intraperitoneal (i.p.) injection gave 97.3 and 98.7% of inhibition. In oral administration, the inhibitory activities were not markedly observed, indicating that the polysaccharides are directly acting to immune system. When the effects on TS and TK activities were determined, TS activities in the F-2 and F-7-treated mice were markedly suppressed to 73.7% and 79.5% of that in the control (p<0.01), while there was little difference in TK activity with a slight decrease in F-2 only. However, in i.p. injection, TS activities in the F-2, F-5, F-7 and F-8-treated mice were markedly suppressed to 83% to 85% of that in the control (p<0.01). Furthermore, there was also significant differences in TK activities in F-2, F-5, F-7 and F-8-treated mice (p<0.05). Therefore, polysaccharide fraction F-8 was further purified to active fractions of F-9 and F-11 by gel permeation chromatography using TSK Gel HW50S. The purified polysaccharides of F-9 and F-11 were composed of GlcNAc (47.3%), Gal (24.7%) and Man (28.0%). These results clearly indicated that the i.p. injection is much effective to suppress tumor growth than oral administration.

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Quantitative Determination of Bakkenolide D in Petasites japonicus and Farfugium japonicum by HPLC/UV

  • Quilantang, Norman G.;Lee, Ki Ho;Lee, Dong Gu;Lee, Ju Sung;Cho, EunJu;Kim, Hyun Young;Lee, Sanghyun
    • Natural Product Sciences
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    • 제23권4호
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    • pp.270-273
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    • 2017
  • A quantitative analysis of bakkenolide D in the different parts of Petasites japonicus and Farfugium japonicum was performed by HPLC. A gradient HPLC elution system with a mobile phase consisting of water: acetonitrile solution (20:80 to 0:100 for 45 min) was followed and an INNO $C_{18}$ column was used for the chromatographic separation. The injection volume, flow rate, and UV detection were $10{\mu}L$, 1 mL/min, and 290 nm, respectively. Results show that both species showed the highest amount of bakkenolide D in the roots being 107.203 and 166.103 mg/g for P. japonicas and F. japonicum, respectively. Content analysis on the different parts of both plants displayed remarkably lower values which ranged from 0.403 - 4.419 and 7.252 - 32.614 mg/g for P. japonicas and F. japonicum, respectively. The results show that the roots of both plants are rich in bakkenolide D showing a promising use in the development of nutraceuticals and industrial application of the compound.

간호대학생의 현장실습적응, 대학적응, 죽음에 대한 인식이 학과적응에 미치는 영향 (Impact on department adaptation of field practice adaptation, college adaptation, and awareness of death of nursing students)

  • 제남주;박미라
    • 한국산학기술학회논문지
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    • 제19권10호
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    • pp.300-312
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    • 2018
  • 본 연구는 간호대학생의 현장실습적응과 대학적응, 죽음에 대한 인식이 학과적응에 미치는 영향을 파악하여 전공적응과 현장실습적응 및 죽음에 대한 인식을 높이기 위한 대책 마련의 기초자료를 마련하고자 시도되었다. 연구대상자는 C시, J시 간호학과 3.4학년 252명을 대상으로 하였다. 자료수집은 C 대학교 IRB의 승인을 받은 후부터 2018년 4월 30일까지였고, 자료분석은 IBM SPSS WIN/21.0을 이용하여 빈도와 백분율, 평균, 표준편차, t-test, ANOVA, pearson correlation coefficient, multiple regression를 사용하였다. 대상자의, 현장실습적응은 평균 3.58점, 대학적응은 평균 3.33점, 죽음에 대한 인식은 평균 3.15점, 간호학과 학과적응은 평균 3.38점이었다. 대상자의 학과적응과 현장실습적응(r=.56, p<.001), 죽음에 대한 부정도(r=-.15, p=.016), 생명존중의지(r=.33, p<.001)와 유의한 상관관계가 있으며, 현장실습적응과 대학적응(r=.48, p<.001), 생명존중의지(r=.16, p=.009)과 상관관계가 있었고, 대학적응과 생명존중의지(r=.32, p<.001)와 유의한 상관관계가 있었다. 간호대학생의 현장실습적응, 대학적응, 죽음에 대한 인식이 학과적응에 미치는 영향을 확인한 결과 가장 큰 영향을 미치는 요인은 대학적응이었으며(F=171.67, p<.001), 이 모형은 67.5%의 설명력을 보였다. 따라서 죽음에 대한 긍정적 태도 변화를 위해 학부 과정에서 죽음에 대한 긍정적 태도 및 인식 변화와 관련된 교육과정이 제공되어야 하며 생명존중의지 프로그램 실시 후 간호학과 적응 효과에 대한 연구가 필요함을 제언한다.

소결온도에 따른 $0.7Mg_4Ta_2O_9-0.3TiO_2$ 세라믹스의 마이크로파 유전특성 (Microwave Dielectric Properties of $0.7Mg_4Ta_2O_9-0.3TiO_2$ Ceramics with Sintering Temperature)

  • 김재식;최의선;이성갑;배선기;이영희
    • 한국전기전자재료학회:학술대회논문집
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    • 한국전기전자재료학회 2003년도 춘계학술대회 논문집 센서 박막재료 반도체 세라믹
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    • pp.249-252
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    • 2003
  • The microwave dielectric properties of $0.7Mg_4Ta_2O_9-0.3TiO_2$ ceramics were investigated. All samples were prepared by the conventional mixed oxide method. The structural properties were investigated with sintering temperature by X-ray Diffractor meter. According to. the X-ray diffraction patterns of the $0.7Mg_4Ta_2O_9-0.3TiO_2$ ceramics, major phase of the hexagonal $Mg_4Ta_2O_9$ were appeared. In the case of $0.7Mg_4Ta_2O_9-0.3TiO_2$ ceramics sintered at $1400^{\circ}C$, dielectric constant, quality factor and temperature coefficient of resonant frequency were 11.72, 126,419GHz, $-31.82ppm/^{\circ}C$, respectively.

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Development of Semen Extenders by Assessment of Sperm Viability in Miniature-Pig Semen

  • Lee S. H.;Cheong H. T.;Yang B. K.;Park C. K.
    • Reproductive and Developmental Biology
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    • 제29권4호
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    • pp.247-252
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    • 2005
  • The purpose of this study was to assess sperm quality during in vitro storage of miniature-pig semen in order to determine which extender should be used and how extender can be diluted for in vitro storage of miniature-pig semen. Freshly ejaculated miniature-pig's semen was diluted with same volumes of Beltsville Thawing Solution (BTS), Androhep, Modena, Mulberry III and modified-Modena extenders. Sperm quality was evaluated by examining viability, motility, abnormality, acrosome intactness, intensity and capacitation status by chlorotetracycline (CTC) staining. Sperm motility decreased with storage period prolonged and differences among BTS, Androhep, Modena and Mulberry III were apparent On Day 1, approximately 80% of the sperm were motile, but motility decreased to $40\%$ at Day 7. During the 7 days of storage, sperm survival in modified-Modena B extender was higher than another extenders. However, it was not differ significantly among other extenders. The percentage of F and B patterns were not differ significantly among the extenders. However, F pattern in modified-Modena B extender was slightly higher until 3 days of storage than that of Modena extender, modified-Modena A extender and modified-Modena C extender. The percentage of AR patterns in modified-Modena B extender was slightly lower, but did not differ significantly among other extenders. The results of present study suggest that modified-Modena B was effective as new extender for in vitro storage of miniature-pig semen.

Restriction Enzyme-Mediated Integration 방법으로 확보한 Fusarium oxysporum 형질전환체의 후자리산 생성능 분석 (Fusaric Acid Production in Fusarium oxysporum Transformants Generated by Restriction Enzyme-Mediated Integration Procedure)

  • 이데레사;신진영;손승완;이수형;류재기
    • 식물병연구
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    • 제19권4호
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    • pp.254-258
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    • 2013
  • 후자린산(FA)는 Fusarium 속 균이 생성하는 독소로서 다른 곰팡이독소보다 독성은 낮으나 다른 독소와 중복 오염시 전체 독성을 증진시키는 것으로 알려졌다. 현재까지 FA 생합성 관련 효소나 유전자가 Fusarium oxysporum에서 밝혀지지 않았기 때문에 본 연구에서는 관련 생합성 유전자의 발굴을 위해 제한효소를 통한 무작위 삽입 형질전환방법인 REMI를 이용하여 FA 생성 F. oxysporum 균주의 생합성유전자의 결손을 시도하였다. F. oxysporum 균주 2주를 대상으로 REMI를 시도한 결과, 평균 3.2주 ($1{\mu}g$ DNA 당)의 효율로 7,100주 이상의 형질전환체를 육성하였다. FA 미생성 형질전환체를 스크리닝 하기 위해 FA가 함유된 배양액에서 다양한 식물종자의 발아여부를 조사한 결과, 11종의 종자 중 가장 감수성인 배추종자를 선발하였다. 각 형질전환체는 Czapek-Dox broth에서 3주간 배양한 후 배양여액을 배추종자의 발아여부 검정에 사용하였다. 검정결과 총 5,000여 주의 REMI 형질전환체 중 53주의 배양여액에서 종자가 발아하지 않아, 이들을 FA 미(저) 생성 추정 형질전환체로 선발하였다. 이중 26주의 FA 생성량을 HPLC로 분석한 결과, 2주의 형질전환체에서 모균주 생성량의 1% 이하의 FA가 검출되었다. 이 중 형질전환체 1주로부터 REMI 벡터 삽입 부위 게놈 DNA의 염기서열(252 bp)을 확보하였으며, 이 부위는 F. fujikuroi의 미동정 게놈부위와 93% 유사성이 있음을 확인하였다. 이 부위의 FA생성 관련성 증명을 위해서는 추후 연구가 필요하다.

울릉도 희귀.특산 식물 섬현호색의 유전적 다양성과 구조 (Genetic Diversity and Structure of a Rare and Endemic, Spring Ephemeral Plant Corydalis filistipes Nakai of Ullung Island in Korea)

  • 김진석;양병훈;정재민;이병천;이재천
    • Journal of Ecology and Environment
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    • 제29권3호
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    • pp.247-252
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    • 2006
  • 울릉도의 특산 식물종으로서 희귀식물종이며, 춘계 단명성 식물로서 개미에 의해 종자 산포가 이루어지는 섬현호색(Corydalis filistipes Nakai) 4개의 아집단에 대하여 합리적인 보전 및 관리 대책 수립을 위하여 9 개의 동위효소 marker를 이용하여 유전적 다양성과 구조를 분석하였다. 그 결과 평균 대립 유전자의 수(A)는 1.73개, 95% 수준에서 다형적 유전자좌의 비율은(P)은 61.2%, 이형접합체의 평균 관측치(Ho)는 0.201, 기대치(He)는 0.167로서 분포역이 넓은 특산 식물 종들에 비해서는 낮은 수준이지만, 섬에 고립된 유사한 생활사를 갖는 특산식물 종들에 비해서는 높은 유전적 다양도를 유지하고 있는데, 그 이유는 소집단이기는 하나 적정수준의 개체수가 유지되고 있고, 타가수정을 주로 하며 개미에 의해 종자 산포가 이루어져 적응력이 높고, 또한 폐쇄화에 의한 유 무성 번식 체계를 겸하기 때문인 것으로 판단된다. 그리고 유전적 구조분석 결과 아집단내($F_{IS}$)와 전체 아집단($F_{IT}$)의 근친교 배계수가 각각 -0.1889 와 -0.1226로서 H-W의 평형구조에 비해 이형접합자의 빈도가 높았으며, 아집단간 유전적 분화도는 매우 낮은($F_{ST}=0.0557$)결과를 보였다. 그리고 우리나라 희귀 및 특산 식물종인 섬현호색의 유전적 변이의 유지 기작과 합리적인 보전과 관리 대책을 논의하였다.