• Journal of Ginseng Research
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• 제22권3호
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• pp.181-187
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• 1998

Nitric Oxide (NO), derived from L-arginine, is produced by two types (constitutive and inducible) of nitric oxide synthase (NOS). The NO produced in large amounts by the inducible NOS is known to be responsible for the vasodilation and hypotension observed in septic shock. We have found three polyacetylene compounds which inhibited the production of NO in LPS-activated RAW 264.7 cells. Their structures were identified as panauynol, ginsenoyne A and PQ-6 by the spec- troscopic analysis (IC50 values were 32.3 $\mu$M, 2.3 $\mu$M, 1.5 $\mu$M, respectively). These polyacetylenes may be useful candidates for the development of new drug to treat endotoxemia and inflammation accompanied by the overproduction of NO.

• 약학회지
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• 제18권4호
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• pp.243-248
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• 1974

Nine benzoxazolin-2-thione derivatives were sunthesized as the potential antimicrobial substances. These compounds were tested for the antimicrobial activities using Staphylococcus aureus and Escherichia coli by way of tube dilution method. The three compounds of I, II and VII omjoboted the growth of S.aureus at the concentration of 1${\mu}$g/ml, and III,VI,VIII and IX exhibited considerable antimicrobial activities against S. aureus at the concentration of 10.m.u.g/ml. As to the growth of E. Coli, VII VII and VIII inhibited at the concentration of 1${\mu}$g/,l. II,III and IV exhibited considerable antimicrobial activities against E. coli at the concentration of 10${\mu}$g/ml.

• Bulletin of the Korean Chemical Society
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• 제11권6호
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• pp.531-534
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• 1990

The reaction of $Os_3(CO)_{10}(NCMe)_2$ with a tungsten alkylidyne $Cp(CO)_2W{\equiv}CTol(Cp={\eta}^5-C_5H_5,\;Tol=p-C_6H_4Me)$ produces the compound, $CpWOs_3(CO)_{11}({\mu}_3-CTol)$(1). The structure of compound 1 can be viewed as one in which the metalla-alkyne, $Cp(CO)_2W{\equiv}CTol$, is ${\mu}_3-{\eta}^2-{\bot}$ (perpendicular) bound to an $Os_3(CO)_9$ fragment. Variable-temperature $^{13}C$ NMR spectra of 1 show no evidence for the analogous rotation of the metalla-alkyne molecule as shown alkyne rotation in $Os_3(CO)_9(C_2Tol_2)$ compound. This lack of the metalla-alkyne fluxionality supports the apparent saturated nature of compound 1.

• 동력기계공학회지
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• 제17권3호
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• pp.72-81
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• 2013

Many articles have been published about a 2-degree of freedom model that includes the lateral and yaw motions for controller synthesis in intelligent transport system applications. In this paper, a 3-degree of freedom linear model that includes the roll motion is developed to design a robust steering controller for lane following maneuvers using ${\mu}$-synthesis. This linear perturbed system includes a set of parametric uncertainties in cornering stiffness and unmodelled dynamics in steering actuators. The state-space model with parametric uncertainties is represented in linear fractional transformation form. Design purpose can be obtained by properly choosing the frequency dependent weighting functions. The objective of this study is to keep the tracking error and steering input energy small in the presence of variations of the cornering stiffness coefficients. Furthermore, good ride quality has to be achieved against these uncertainties. Frequency-domain analyses and time-domain numerical simulations are carried out in order to evaluate these performance specifications of a given vehicle system. Finally, the simulation results indicate that the proposed robust controller achieves good performance over a wide range of uncertainty for the given maneuvers.

• 한국동물위생학회지
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• 제41권3호
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• pp.171-177
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• 2018

Purified bee venom was collected from colonies of honeybees (Apis mellifera L.) using a bee venom collector under sterile conditions and then purified under strict laboratory conditions. Purified bee venom contained $63.9{\pm}5.4%$ melittin, $10.9{\pm}1.6%$ phospholipase A2, and $2.3{\pm}0.3%$ apamin. Purified bee venom has various anti-bacterial, anti-inflammatory and immunostimulating effects. In this study, we evaluated purified bee venom which are mammary gland cells, MAC-T cells are used to increase the synthesis of milk protein. Purified bee venom promoted the proliferation of MAC-T cells at concentrations below $1{\mu}g/mL$, but cytotoxicity at $10{\mu}g/mL$ and above. As a result of the increase in the synthesis of ${\beta}-casein$, a milk protein after treatment with MAC-T cells at a concentration of the bee venom without cytotoxicity, the ${\beta}-casein$ content in the cell culture was increased when treated at a concentration of 1 ng/mL or more. In addition, it was confirmed that purified bee venom significantly increased the expression of bovine ${\beta}-casein$ (bCSNB) mRNA, a ${\beta}-casein$ synthesis gene, at a concentration of 1 ng/mL or more. These results suggest that purified bee venom can be used to increase the production of livestock by ultimately increasing the expression of milk protein.

• 한국결정성장학회지
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• 제11권5호
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• pp.185-189
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• 2001

비정질 인산알루미늄 분말은 화학양론조성비의 $Al_2(SO_4)_3$와 $H_3PO_4$혼합물을 NaOH 또는 KOH 용액을 이용하여 중화 반응시킨 뒤 이를 수열침전법에 의해 단일상으로 얻을 수 있었으며, 합성조건은 다음과 같다. 즉, 출발원료; $Al_2(SO_4)_3$와 $H_3PO_4$, 중화반응의 pH범위; 5.6~6.0, 수열반응의 온도범위; 170~$180^{\circ}C$, 수열반응의 시간범위; 4~5시간이었다. 이와 같은 조건하에서 얻어진 생성물은 0.1~0.3$\mu\textrm{m}$ 크기의 비정질 인산알루미늄 미립자였으며 그리고 미국약전에 따라 실험한 결과 모두 적합하였다.

• Journal of Applied Biological Chemistry
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• 제54권4호
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• pp.284-289
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• 2011

선학초 추출물을 기능성 화장품 소재로 활용하기 위하여 미백효과를 검증하였다. 세포 내 tyrosinase 저해활성 측정결과 선학초 에탄올 추출물 $500{\mu}g/mL$에서 42%의 저해활성을 나타내었다. 이는 선학초 추출물이 세포 내 tyrosinase 발현을 억제시킴으로서 멜라닌 합성 또한 저해 하는 것임을 확인 할 수 있었다. 선학초 추출물의 단백질 발현과 mRNA 발현 억제효과를 검토한 결과 선학초 열수 및 에탄올 추출물을 처리한 B16F10군에서는 tyrosinase protein의 발현이 처리하지 않은 군보다 감소함을 확인할 수 있었다. 결과적으로 선학초 추출물의 미백효능을 확인할 수 있었으며, 식품 및 화장품의 기능성 소재로 이용이 가능할 것으로 판단된다.

• 대한화장품학회지
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• 제40권4호
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• pp.341-347
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• 2014

본 연구에서는 노무라입깃해파리(Nemopilema nomurai) 가수분해 추출물의 미백활성에 대하여 검색하였다. 가수분해 시료(NHE, N. nomurai Hydrolized Extract)는 노무라입깃해파리를 상업용 단백질분해효소(Protamex, Alcalase, Flavourzyme, Neutrase)를 이용하여 $55^{\circ}C$에서 pH 5-8 조건으로 처리하는 과정을 거쳐 얻었다. 미백활성은 B16-F1 멜라노마 세포에서 멜라닌 색소 합성 저해능 평가를 통하여 확인하였다. 실험결과, Neutrase로 처리된 시료(N-NHE)에서 $100{\mu}g/mL$의 농도에서 멜라닌 생성이 가장 효율적으로 89.9% 감소되는 것을 관찰하였다. 이 시료는 또한 tyrosinase와 TRP-1 (tyrosinase related protein-1) 단백질의 발현을 억제하였다. 이상의 연구 결과는 노무라입깃해파리 가수분해 추출물의 화장품 미백 소재로의 개발 가능성을 보여주고 있다.

• Molecules and Cells
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• 제26권6호
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• pp.625-630
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• 2008

This study was undertaken with the aim of developing an easy and quick means of analyzing the effect of various compounds on the synthesis and secretion of human type I collagen at the protein level. A modification of the ELISA method was used on HFF-1 cells. For the proof of concept, we used thirteen compounds most of which are known to be antioxidants. Each compound was tested at concentrations of 0, 10 and $100{\mu}m$ on HFF-1 cells for 24 h. Thirteen sets of experiments for each compound were performed in ANOVA with three replicates. Duncan multiple range test (DMRT) was used to compare the mean values obtained from the treatment groups. From the results it was concluded that Vitamin C, undecylenic acid, conjugated linoleic acid, glycolic acid, and citric acid at $100{\mu}m$ concentration could be used for anti-wrinkling or protection from premature aging, which requires enhancement of collagen synthesis. Lactic acid, EGCG, resveratrol, and retinol that can inhibit collagen synthesis effectively in a dose-dependent manner may be used for anti-fibrosis treatment purposes.

• 공업화학
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• 제20권6호
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• pp.617-621
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• 2009

Zinc oxide (ZnO)을 금(Au)과 fluorine-doped tin oxide (FTO) 촉매로 산화실리콘($SiO_2$) 기판에 산화아연입자 20 nm, $20{\mu}m$를 각각 사용하여 기체-액체-고체(VLS) 합성법으로 성장시켰다. 나노로드의 표면특성, 화학조성, 그리고 결정특성을 엑스레이회절(X-ray diffraction (XRD)), 에너지 분산형 X선 분광기(Energy-dispersive X-ray spectroscopy (EDX)), 표면 방출주사현미경(Field-emission scanning electron microscope (FE-SEM))으로 분석하였다. ZnO의 입자 크기 뿐만 아니라 결정형태가 성장에 크게 영향을 미쳤다. ZnO의 모든 나노구조가 6방정계(六方晶系), 단일결정구조를 가지고 있었다. 최적온도는 $1030^{\circ}C$, 입자크기는 20 nm이다. 그러므로 Au 대신 플루오린 첨가 도핑으로 전기음성도가 증가된 FTO 증착에 의해서 생성된 나노로드는 경제성 있는 대체물질로서의 가치가 있을 것으로 사료된다.