• Applied Biological Chemistry
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• 제48권4호
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• pp.315-321
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• 2005

유색미 겨 추출물이 염증반응에 미치는 효과를 호중구 세포주 및 비만세포가 분비하는 염증매개물질인 histamine과 ${\beta}-hexosaminidase$의 분비 및 염증 cytokine 생성에 대한 억제활성을 측정하여 평가하였다. 호중구 세포주인 RBL-2H3 세포와 rat에서 분리 추출한 복강 비만세포(RPMC, peritoneal mast cell)를 이용하여 histamine과 ${\beta}-hexosaminidase$ 분비에 대한 억제활성을 조사한 결과, 일반미 겨 추출물은 RBL-2H3 세포에서 염증매개물질들의 분비량을 증가시킨 반면, 유색미 겨 추출물은 이들의 분비를 억제하였다(histamine 10.19% 억제, ${\beta}-hexosaminidase$ 110.03% 억제). RPMC에서는 유색미가 histamine과 ${\beta}-hexosaminidase$의 분비를 일반미보다 각각 8배와 3배나 높게 억제하는 것으로 나타났다. 염증관련 cytokine으로서 $IL-1{\beta},\;TNF-{\alpha}$ 및 IL-6의 발현을 RT-PCR로 측정한 결과, 유색미가 일반미 보다 $TNF-{\alpha},\;IL-1{\beta}$ 및 IL-6의 발현을 억제하는 효과가 우수하였다. ELISA를 이용하여 cytokine의 분비량을 측정한 실험에서도 유색미가 일반미보다 $TNF-{\alpha},\;IL-1{\beta}$ 및 IL-6의 분비를 효과적으로 억제하였다. 이상의 결과는 유색미가 염증반응의 원인 물질인 histamine과 ${\beta}-hexosaminidase$의 분비에 대한 억제효과 뿐 아니라, 염증 cytokine의 발현을 저해하는 효과도 일반미보다 우수하다는 사실을 보여주었다.

• Journal of Applied Biological Chemistry
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• 제50권3호
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• pp.155-159
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• 2007

The water extracts of root, stem, and leaf from Korean indigenous Acanthopanax divaritacus were examined for their suppressive effects against allergic inflammations such as lipoxygenase activity, ${\beta}-hexosaminidase$ release, inflammatory cytokine production, and serum IgE level. The root extract inhibited the release of ${\beta}-hexosaminidase$, a degranulation marker, from rat basophilic leukemia cells (RBL-2H3) much more potently than the stem and leaf extracts. The root extract also significantly reduced the expression of $TNF-{\alpha}\;and\;IL-1{\beta}$ in the RBL-2H3 cells challenged with antigen. Moreover, there was a significant fall in the serum IgE level by the treatment of the root extract. Taken together, the root extract could be the most potent inhibitor of allergic inflammation, suppressing ${\beta}-hexosaminidase$ release and inflammatory cytokine expression, as well as reducing the rise of serum IgE level.

• 한국약용작물학회지
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• 제18권4호
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• pp.231-237
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• 2010

The present study was conducted to investigate the anti-allergic reaction with Glycyrrhiza uralensis. We examined cell viability, $\beta$-hexosaminidase release, IL-4 and IL-13 mRNA expression from RBL-2H3 cell after pre-treatment with 0, 100, 250, 500, 1000${\mu}g/m{\ell}$ of Glycyrrhiza uralensis water extracts. Effects of Glycyrrhiza uralensis on the degranulation and pro-inflammatory cytokines (IL-4 and IL-13) expression were evaluated with $\beta$-hexosaminidase assay, and RT-PCR analysis. We observed that Glycyrrhiza uralensis concentrations from 100${\mu}g/m{\ell}$ to 1000${\mu}g/m{\ell}$ had no effect on cell survival. The release of $\beta$-hexosaminidase decreased significantly with all concentrations of Glycyrrhiza uralensis extracts. The expression of the IL-4 and IL-13 mRNA were decreased by Glycyrrhiza uralensis in dose-dependent manner. These results that Glycyrrhiza uralensis has an anti-histamin effects and controls IL-4, IL-13 secretion on allergic reaction.

• The Korean Journal of Physiology and Pharmacology
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• 제15권5호
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• pp.313-317
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• 2011

The effects of extremely low frequency electromagnetic fields (EMF) on intracellular $Ca^{2+}$ mobilization and cellular function in RBL 2H3 cells were investigated. Exposure to EMF (60 Hz, 0.1 or 1 mT) for 4 or 16 h did not produce any cytotoxic effects in RBL 2H3 cells. Melittin, ionomycin and thapsigargin each dose-dependently increased the intracellular $Ca^{2+}$ concentration. The increase of intracellular $Ca^{2+}$ induced by these three agents was not affected by exposure to EMF (60 Hz, 1 mT) for 4 or 16 h in RBL 2H3 cells. To investigate the effect of EMF on exocytosis, we measured beta-hexosaminidase release in RBL 2H3 cells. Basal release of beta-hexosaminidase was $12.3{\pm}2.3%$ in RBL 2H3 cells. Exposure to EMF (60 Hz, 0.1 or 1 mT) for 4 or 16 h did not affect the basal or $1{\mu}m$ melittin-induced beta-hexosaminidase release in RBL 2H3 cells. This study suggests that exposure to EMF (60 Hz, 0.1 or 1 mT), which is the limit of occupational exposure, has no influence on intracellular $Ca^{2+}$ mobilization and cellular function in RBL 2H3 cells.

• 한국버섯학회지
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• 제12권4호
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• pp.324-329
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• 2014

본 연구에서는 버섯추출물 (42개)의 in vitro 항알레르기 효능탐색을 위해 랫드비만세포 (RBL-2H3 cell)에서 면역글로불린 (IgE)가 매개한 탈과립에 대한 저해 효과를 실험하였다. 이를 위해 anti-DNP IgE 및 DNP-HSA에 의해 알레르기반응이 유발된 랫드비만세포에서 버섯 추출물의 IL-4과 ${\beta}$-hexosaminidase 분비량에 대한 저해활성과 세포생존에 대한 영향이 분석되었다. 실험결과, IL-4 분비에 대해서는 팽이버섯 물추출물 등 5개의 버섯 추출물이 20% 이상의 우수한 저해효과를 나타내었으며, ${\beta}$-hexosaminidase 분비에 대해서는 영지버섯의 물추출물 등 8개의 버섯 추출물이 20% 이상의 비교적 우수한 저해활성을 나타내었다. 세포증식에 대해서는 잎새버섯의 물추출물등 대부분의 버섯 추출물이 우수한 세포증식효과를 나타내었다. 팽이버섯의 물추출물과 상황버섯의 물추출물은 ${\beta}$-hexosaminidase 및 IL-4 분비에 대해 모두 비교적 우수한 저해효과를 나타내었다. 추가로 2, 10, 50 ug/ml에서 실험된 영지버섯, 편각영지버섯, 눈꽃동충하초, 상황버섯 그리고 느타리버섯의 물추출물들은 ${\beta}$-hexosaminidase의 분비량을 농도-의존적으로 감소시켰다. 이상의 결과를 살펴볼때, 이들 in vitro 항알레르기 효과를 나타낸 버섯 추출물들은 추가실험을 통해 항알레르기 소재로의 활용성 검토가 필요하다고 사료되었다.

• 대한화장품학회지
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• 제40권1호
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• pp.21-28
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• 2014

본 연구에서는 두충 추출물이 RBL-2H3 세포의 ${\beta}$-hexosaminidase의 분비 억제와 HaCaT keratinocytes 피부장벽의 회복과 관련한 filaggrin, transglutaminase-1 (TGase-1), cornified cell envelope(CE)의 발현에 미치는 영향에 관하여 연구하였다. ${\beta}$-hexosaminidase 방출 억제 능은 13% 효능을 확인하였고, 피부장벽기능의 회복과 관련된 인자들은 발현과 활성의 정도가 매우 우수한 것을 확인하였다. 각질형성세포의 분화를 판단할 수 있는 CE 측정에서는 두충추출물이 양성대조군보다 더 좋은 효능을 나타내기도 하였다. 따라서 두충 추출물은 ${\beta}$-hexosaminidase 분비 억제에 효과가 있으며, 손상된 피부장벽강화에 영향을 미치는 각질형성세포의 분화 촉진에 효과가 있음을 확인하였다.

• 한국식품과학회지
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• 제41권6호
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• pp.717-721
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• 2009

고장초 메탄올 추출물의 항알레르기 활성을 조사하기 위해, 비만세포주인 RBL-2H3 세포를 이용하였으며, 고장초 추출물을 처리한 후 IgE-항체, A23187 및 PMA를 이용하여 세포를 활성화시켜 분비되는 $\beta$-hexosaminidase, TNF-$\alpha$의 양을 측정하였다. 그 결과 고장초 추출물이 활성화된 비만세포에서 분비되는 $\beta$-hexosaminidase, TNF-$\alpha$를 농도 의존적으로 억제하는 것을 확인할 수 있었다. 또한, 비만세포에 탈과립이 발생할 경우 나타나는 형태학적 변화에 대한 시료의 영향을 검색하기 위해 고장초 추출물을 처리한 세포에 compound 48/80을 처리하였으며, 그 결과 고장초 추출물을 처리한 경우 대조군에 비해 탈과립이 현저히 억제되는 것을 관찰하였다. 따라서 고장초 추출물은 다양한 자극으로 인한 비만세포의 탈과립 저해를 통해 알레르기 반응을 억제시키는 것으로 판단되며, 본 연구결과는 고장초의 항알레르기 기능성 소재로의 이용 가능성을 시사해준다.

• Journal of Applied Biological Chemistry
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• 제53권3호
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• pp.139-146
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• 2010

The inhibitory effects of 25 polyphenols against in vitro allergic reactions were compared using biochemical and cell assays. Three polyphenols including curcumin, gallic acid, and quercetin suppressed the release of $\beta$-hexosaminidase from ionophore A23187-stimulated RBL-2H3 cells more effectively (>50% inhibition at $100{\mu}M$ concentration). They were found to have potencies in suppressing the release of histamine not only from ionophore A23187-, but also from immunoglobulin E (IgE)-stimulated RBL-2H3 cells. Moreover, such suppressive effects of the three polyphenols were also observed in A23187 plus PMA-costimulated rat peritoneal mast cells. The extent of inhibition were quantified as the respective polyphenol concentration that inhibit 50% ($IC_{50}$) of $\beta$-hexosaminidase or histamine release, showing an inhibition tendency with decreasing order of curcumin>gallic acid>quercetin. Down-regulation of $Ca^{2+}$ influx was suggested as the cause of the inhibition of $\beta$-hexosaminidase and histamine releases in these cells. The immune process inhibition was confirmed by the observed reduction in the gene expressions and release of pro-inflammatory cytokine tumor necrosis factor (TNF)-$\alpha$, interleukin (IL)-$1\beta$, and IL-4, due probably to antioxidant activity of the polyphenols. These findings illustrate that curcumin, gallic acid, and quercetin may be beneficial against allergic inflammatory diseases.

• Korean Journal of Acupuncture
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• 제28권3호
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• pp.73-83
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• 2011

Objectives : The purpose of this study is to investigate anti-allergic effects of Glycyrrhizae Radix(GR) pharmacopuncture and GR extract. Methods : In vivo, animals were gotten GR pharmacopunctures at both sides of ST36s three times for 5 days. Then, we investigated anti-DNP IgE-induced passive cutaneous anaphylaxis of Sprague Dawley rats. In vitro, we measured cell viability, ${\beta}$-hexosaminidase release, and the secretion of interleukin-4(IL-4) and tumor necrosis factor-alpha(TNF-${\alpha}$) in RBL-2H3 cells after treatment of various concentrations of GR extract. Results : In vivo, we observed inhibition of passive cutaneous anaphylaxis after GR pharmacopuncture treatments at both sides of ST36s and optional points. In vitro, GR extract treatments did not affect cell viability, but inhibited ${\beta}$-hexosaminidase release and the secretion of IL-4 and TNF-${\alpha}$. Conclusions : These results suggest that GR pharmacopuncture and GR extract should be beneficial in the inhibition of allergic inflammatory response.

• 한국축산식품학회지
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• 제38권4호
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• pp.780-793
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• 2018

This study was conducted to compare the anti-allergic effects of a whey protein concentrate (WPC) and WPC hydrolysate. WPC hydrolysate was prepared using enzymatic digestion for 8 h with trypsin and ${\alpha}$-chymotrypsin, after which it was freeze-dried. The allergic parameters assessed in rat basophilic leukemia (RBL)-2H3 cells were degranulation and release of ${\beta}$-hexosaminidase, release of tumor necrosis factor $(TNF)-{\alpha}$, and changes in the expression of $IL-1{\beta}$, IL-4, and IL-10 by real time polymerase chain reaction (PCR). During preparation of the WPC hydrolysate, hydrolysis increased rapidly from 0 to 10 min and then gradually increased slowly from 1 h onwards, achieving a final degree of hydrolysis of 78.50%. The SDS-PAGE analysis revealed a reduction in the intensity of several protein bands in the WPC hydrolysate compared to the WPC. IgE-induced ${\beta}$-hexosaminidase release from RBL-2H3 cells was decreased to a higher degree following treatment with the hydrolysate compared to WPC treatment. W500 ($500{\mu}g/mL$ WPC) showed the least inhibition of ${\beta}$-hexosaminidase release, but there was no significant difference between W500 and W1000 ($1,000{\mu}g/mL$) (p<0.05). H1000 ($1,000{\mu}g/mL$ WPC hydrolysate) inhibited ${\beta}$-hexosaminidase release by 39%. Compared to the control, treatment with H1000 decreased $TNF-{\alpha}$ secretion to 11.87 pg/mL. The gene expression levels of IL-1${\beta}$, IL-4, and IL-13 were all significantly decreased in hydrolysate (p<0.05). In the case of $IL-1{\beta}$ and IL-4, the expression levels in W1000 treated cells were decreased by 73.67% and 65%, respectively, and that of IL-13 was decreased by 66.43% compared to the control.