• Analytical Science and Technology
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• v.33 no.1
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• pp.11-22
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• 2020

When extracting semi-volatile components of herbal medicines using hot water vapor, some substances may react with water vapor or oxygen, and some volatile substances may be lost, when using an organic solvent extraction method has the disadvantage that it may contain a non-volatile material and residual organic solvent. In addition, it is inefficient to separate semi-volatile substances from herbal medicines into each single component and conduct biological activity research for each component to determine the effective ingredient, and some components may be lost in the separation process. In this study, semi-volatile substances evaporated under two pressure-reduced conditions in Chinese herbal medicines such as Cnidii Rhizoma, Aucklandiae Radix and Amomum Fructus were separated by cooling with liquid nitrogen. Those were analyzed by gas chromatography-mass spectrometry (GC-MS) to identify the components, and this method may be used to study biological activities at the cellular level. The substances separated under reduced pressure, essential oil obtained by simultaneous distillation extraction (SDE) method and substances by using solid phase micro-extraction (SPME) from Cnidii Rhizoma, Aucklandiae Radix and Amomum Fructus were analyzed by GC-MS. In the case of Cnidii Rhizoma and Aucklandiae Radix, there were some differences among the essential oil components obtained by SDE and those identified by low temperature capture (CT) and SPME method, these were believed to be produced by some volatiles reacting with water or oxygen at the boiling point temperature of water.

• The Korean Journal of Food And Nutrition
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• v.34 no.2
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• pp.196-206
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• 2021

The main purpose of this study was to examine the correlation between the consumption of red ginseng-based 'SSR' for 30 days and the reduction in human fatigue, blood component changes, and immune cell activity in 35 human subjects. 'SSR' is composed of zinc oxide, folic acid, and D-α-tocopherol with red ginseng as the main component. According to the protocol criteria of the study, 35 subjects who understood the purpose of the study and signed an informed consent form were selected. The fatigue survey was conducted through a questionnaire, and after taking 'SSR', a decreased tendency of physical, mental, and neurosensory fatigue was observed. In hematological analysis, no significant changes were observed in the levels of WBC, RBC, and hemoglobin; however, AST (SGOT) and ALT (SGPT) levels were statistically significantly decreased. In immunological analysis, it was observed that the proliferative effect of T cells (CD3+CD4+) was greater than that of NK cells (CD16+CD56+). The collected data were subjected to t-test analysis using the SPSS 25.0 statistical program. The result from this study proposes that 'SSR' can be used as a functional food material as it reduces human fatigue and enhances immune function.

• Analytical Science and Technology
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• v.27 no.6
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• pp.284-291
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• 2014

The handling process of car indoor air quality is composed of 2 steps of testing. First, assambly part is tested to find a source of car indoor VOCs. Second, cut sample of unit component is tested to find a source material of VOCs emission. If the source material of VOCs emission is found, it can reduce car and assembly part of VOCs by improving material. As cut sample testing has problem of emission from cut surface to find the source of VOCs, it needs to apply unit components testing method. The aim of study is to evaluate VOCs impact factor of unit components in assembly parts. ISO 12219-5 test method reflects not only material effect but also surface area effect by testing unit component without cutting. The unit components of doortrim and console, were tested by ISO12219-5. And it could figure what unit component is main source of VOCs in assembly. And quantity conversion Factor which gets by testing assembly and unit components can be used to make guideline of ISO 12219-5.

• Analytical Science and Technology
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• v.33 no.2
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• pp.86-97
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• 2020

Reconstruction and separation of explosive-contaminated overlapping fingerprints constitutes an analytical challenge of high significance in forensic sciences. Laser-induced breakdown spectroscopy (LIBS) allows real-time chemical mapping by detecting the light emissions from laser-induced plasma and can offer powerful means of fingerprint classification based on the chemical components of the sample. During recent years LIBS has been studied one of the spectroscopic techniques with larger capability for forensic sciences. However, despite of the great sensitivity, LIBS suffers from a limited detection due to difficulties in reconstruction of overlapping fingerprints. Here, the authors propose a simple, yet effective, method of using chemical mapping to separate and reconstruct the explosive-contaminated, overlapping fingerprints. A Q-switched Nd:YAG laser system (1064 nm), which allows the laser beam diameter and the area of the ablated crater to be controlled, was used to analyze the chemical compositions of eight samples of explosive-contaminated fingerprints (featuring two sample explosive and four individuals) via the LIBS. Then, the chemical validations were further performed by applying the Raman spectroscopy. The results were subjected to principal component and partial least-squares multivariate analyses, and showed the classification of contaminated fingerprints at higher than 91% accuracy. Robustness and sensitivity tests indicate that the novel method used here is effective for separating and reconstructing the overlapping fingerprints with explosive trace.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2021.04a
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• pp.5-5
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• 2021

Alcoholic and nonalcoholic steaohepatitis is a leading form of chronic liver disease with few biomakers ad treatment options currently available. a progressive disease of NAFLD may lead to fibrosis, cirrhosis, and hepatocellular carcinoma. Recently, we extracted HIMH0021, which is an active flavonoid component in the Acer tegmentosum extract, has been shown to protect against liver damage caused by hepatic dysfunction. Therefore, in this study, we aimed to investigate whether HIMH0021 could regulate steatohepatitis and liver fibrosis during alcoholic or nonalcoholic metabolic process. HIMH0021, which was isolated from the active methanol extract of A. tegmentosum, inhibited alcohol-induced steatosis and attenuated the serum levels of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) during hepatocellular alcohol metabolism, both of which promote lipogenesis as well as liver inflammation. Treatment with HIMH0021 conferred protection against lipogenesis and liver injury, inhibited the expression of cytochrome P4502E1, and increased serum adiponectin levels in the mice subjected to chronic-plus-binge feeding. Furthermore, in hepatocytes, HIMH0021 activated fatty acid oxidation by activating pAMPK, which comprises pACC and CPT1a. These findings suggested that HIMH0021 could be used to target a TNFα-related pathway for treating patients with alcoholic hepatitis.

• Journal of fish pathology
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• v.36 no.2
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• pp.349-360
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• 2023

Common carp (Cyprinus carpio) (Weight 23.05 ± 6.98 g, Length 12.65 ± 1.38 cm) were exposed to waterborne nitrite at 0, 50, 100, 200, 400 and 800 mg NO₂ ^-/L for 96 hours. The lethal concentration 50 (LC₅₀) of C. carpio exposed to waterborne nitrite was 398.6 mg NO₂ ^-/L. Hemoglobin, hematocrit and RBC count were significantly decreased by waterborne nitrite exposure. The MCV (mean mean corpuscular volume) (µl), MCH (mean corpuscular hemoglobin) (pg) and MCHC (mean corpuscular hemoglobin concentration) (%) were significantly increased. The inorganic component, plasma calcium, was significantly increased, and the organic components such as plasma cholesterol and total protein were significantly increased showing a similar tendency with calcium. In enzymatic components, the AST and ALT were also significantly increased by nitrite exposure. The results of this study indicate that exposure to nitrite can affect the survival and hematological physiology of C. carpio.

• Analytical Science and Technology
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• v.30 no.5
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• pp.234-239
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• 2017

Identifying the components of residues that are not completely burned at the sites of fires site can provide valuable information for tracing the causes of fires. In order to clarify the types of plastic combustion residues found at the scenes of fires, we studied the residue formed after the combustion of polyethylene (PE) and acrylonitrile butadiene styrene (ABS). Plastic samples were burned at 200, 300, 350, 400, and $500^{\circ}C$ for 3 min using a cone calorimeter, and the changes in weight and combustion products were observed. The powder products obtained by lyophilization and pulverization of the combustion products obtained at each temperature were analyzed by a Fourier transform-near infrared (FT-NIR) spectrometer. When the PE samples were burned, the weight did not change up to $350^{\circ}C$, however a significant change in the weight could be measured above $400^{\circ}C$. The principal component analysis (PCA) of the FT-NIR spectra of the PE and ABS samples obtained at each temperature confirmed that the combustion residues at each temperature were PE and ABS, respectively. Therefore, the types of unburned plastics found at the sites of fires can be confirmed rapidly by near infrared spectroscopy.

• Analytical Science and Technology
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• v.30 no.5
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• pp.240-251
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• 2017

1,1,1,2-Tetrafluoroethane (HFC-134a), which is used as refrigerant in air conditioners, has been recently regulated as a greenhouse gas and is recommended for reuse by refining. It is very important to quantitatively analyze trace impurities present in the refrigerant to evaluate the criteria for reuse. In this study, trace impurities including C, H, Cl, and F, which are difficult to quantify because there are no reference materials, were quantitatively analyzed by a gas chromatograph-atomic emission detector (GC/AED); for this analysis, this was preceded by a qualitative analysis with a GC-mass selective detector (GC/MSD). In addition, the AED response was investigated using a hydrocarbon mixed reference material, which was proportional to the number of atoms in the component. Fifteen refrigerant components were detected as trace impurities in HFC-134a by qualitative analysis of trace impurities including C, H, Cl, and F in the samples. Based on the results of the qualitative analysis, quantitative analysis of trace impurities using AED showed that the highest mole fractions were for the $CHClF_2$ component ($45438.38{\mu}mol/mol$) in one sample and for the $C_2H_2ClF_3$ component ($1311.47{\mu}mol/mol$) in another sample. From this study, it has been shown that it is possible for this analytical method to be applied to the qualitative and quantitative analysis of trace compounds in refrigerants, which are difficult to quantify because of the absence of reference materials.

• Analytical Science and Technology
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• v.21 no.2
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• pp.123-128
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• 2008

The golden turbid solution of cordierite precursor was obtained by using magnesium ethoxide in sol-gel method, while the clear solution of cordierite precursor was obtained when 5%-$Zr(OC_3H_7)_4$ solution was used in the sol-gel reaction. $SiO_2$ component was confirmed by infrared spectra showing $1045cm^{-1}$ and the stretching vibration of gelish $SiO_4$ showed $1140cm^{-1}$ and $940cm^{-1}$. The component of $Al_2O_3$ showed at $580cm^{-1}$ and network structure of $Al_2O_6$ showed at $680cm^{-1}$. The component of MgO was confirmed at $575cm^{-1}$ as the stretching vibration. X-ray diffraction analysis showed ${\mu}$-cordierite crystal was showed up at temperature above $1000^{\circ}C$ at the mole ratio of cordierite precursor and water (1:5). ${\mu}$-Cordierite and ${\alpha}$-cordierite were coexisted at $1050^{\circ}C$ for the mole ratio of cordierite precursor and ammonia (1:5) while ${\alpha}$-cordierite was only existed at $1100^{\circ}C$ for the same mole ratio as mentioned above.

• Korean Journal of Poultry Science
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• v.43 no.3
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• pp.143-148
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• 2016

This study was conducted to investigate the effect of dietary egg yolk antibody (IgY) powder supplementation on the growth performance, blood component profile, intestinal microflora, and immunoglobulin G in ducks. A total of 300 1-day-old ducks (Cherry Valley) were randomly divided into 5 groups, with 3 replicates of 20 birds. The treatment groups were the negative (NC), positive (PC), egg yolk antibody powder 0.1% (T1), egg yolk antibody powder 0.5% (T2), and egg yolk antibody powder 1.0% (T3) groups. In the growth performance of ducks during the entire experimental period, the IgY groups and positive control group (PC) showed significantly higher (P<0.05) body weights and body weight gains compared to the negative control group (NC). However, no significant differences were observed in the feed intake and feed conversion ratio. The blood component profiles showed that the IgY 0.1 and 1.0% groups decreased in total cholesterol content compared to the NC group. The aspartate aminotransferase (AST) and alanine aminotranferease (ALT) contents were lower in the IgY 1.0% group, but there were no significant differences. Regrading the leukocyte content after feeding IgY, the heterophil: lymphocyte ratio decreased in the IgY groups, especially in the IgY 0.1% group, which had a lower content than the other groups. However, these results showed no significant differences. The Lactobacillus count in the intestines significantly increased (P<0.05) in the IgY 0.1 and 0.5% groups, the level of IgY increased, and the Escherichia coli count decreased. However, no significant difference was observed in the total plate count. The immunoglobulin G content was lower in the IgY groups than in the NC group, and compared with the IgY groups, the IgY 0.5% had a lower content, which was not a significant difference.