• 재활간호학회지
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• 제1권1호
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• pp.93-109
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• 1998

The purpose of study is to identify the relation between pain level and perceived family support and quality of life in musculoskeletal patient with chronic pain. The subjects for the study consist of 155 patients with musculoskeletal pain that received medical treatment in hospital or by attending hospital in Chonju. The data were collected during the period from August 5 to August 14, 1998 by means of interviews with structured questionnaire. Data analysis was done by descriptive statistics. t-test, ANOVA, Pearson's correlation, Regression. Cronbach alpha using the SAS program. The result of this study were as follows : 1. The mean score of pain was 8.02, family support was 3.88 and quality of life was 3.07. 2. Hypothesis : The first hypothesis that 'The lower pain level is, the higher quality of life is' was accepted (r=-.2178, p= .0065). In addition, pain level of musculoskeletal patient with chronic pain provided predicted 4.7%(F=7.619, P= .0065) of quality of life. The second hypothesis that 'The higher perceived family support is, the lower pain level is' was rejected (r=-.0376, p= .6425). The third hypothesis that 'The higher perceived family support is, is higher quality of life is' was accepted (r= .3212, p= .0001). In addition, perceived family support of musculoskeletal patient with chronic pain provided predicted 10.31% (F=17.597, p= .0001) of quality of life. 3. General characteristics related pain were age(F=6.85, p= .0001),educational-level(F=9.29, p= .0001), occupation(F=5.81, p= .0037), marriage status(F=8.09, p= .0005), family numbers(F=5.73, p= .001), benefits of medical care(F=4.09, p= .0019), pain period(F=9.52, p= .0001), part of pain(F=2.33, p= .0352), pain period(F=3.08, p= .0181). 4. General characteristics related pain were sex(t=3.20, p= .0017), support sources(t=3.26, p= .0014), pain period(F-4.52, p= .0018). 5. General characteristics related pain were religion(t=3.11. p= .0022), benefits of medical care(F=3.61, p= .0293), pain duration(F=3.03, p= .0195). In conclusion, perceived family support in musculoskeletal patient with chronic pain is an important factor that can improve their quality of life. Therefore, nurses must establish nursing plan included patient's family when nurses carry out nursing intervention and education for patient so that a patient promote quality of life by maintaining optimal wellbeing.

• Journal of Microbiology and Biotechnology
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• 제22권4호
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• pp.494-500
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• 2012

Silk fibroin (SF) peptide has been traditionally used as a treatment for flatulence, spasms, and phlegm. In this study, we examined whether SF peptide enhanced the anti-inflammatory effect of PEP-1-FK506 binding protein (PEP-1-FK506BP) through comparing the anti-inflammatory activities of SF peptide and/or PEP-1-FK506BP. In the presence or absence of SF peptide, transduction levels of PEP-1-FK506BP into HaCaT cells and mice skin and anti-inflammatory activities of PEP-1-FK506BP were identified by Western blot and histological analyses. SF peptide alone effectively reduced both mice ear edema and the elevated levels of cyclooxygenase-2, interleukin-6 and $-1{\beta}$, and tumor necrosis factor-${\alpha}$, showing similar anti-inflammatory effect to that of PEP-1-FK506BP. Furthermore, co-treatment with SF peptide and PEP-1-FK506BP exhibited more enhanced anti-inflammatory effects than the samples treated with SF peptides or PEP-1-FK506BP alone, suggesting the possibility that SF peptide and PEP-1-FK506BP might interact with each other. Moreover, the transduction data demonstrated that SF peptide did not affect the transduction of PEP-1-FK506BP into HaCaT cells and mice skin, indicating that the improvement of anti-inflammatory effect of PEP-1-FK506BP was not caused by enhanced transduction of PEP-1-FK506BP. Thus, these results suggest the possibility that co-treatment with SF peptide and PEP-1-FK506BP may be exploited as a useful therapy for various inflammation-related diseases.

• 대한한방내과학회지
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• 제25권4호
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• pp.260-273
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• 2004

Backgrounds : In recent years, asthma has become recognized as a chronic inflammatory disease associated pathologically with airway epithelial inflammation and airway remodeling. Objectives : To evaluate the different effects of Hirudo depending upon pharmaceutical manufactures on the expression and the activities of IL-6 and GM-CSF in airway epithelial cells, samples of Hirudo(水蛭), Hirudo toasted with Talcum(水蛭滑石炒) and Hirudo toasted with Ephedrae Herba(水蛭麻黃炒) were tested. Methods : After inducing enhanced messenger RNA(mRNA) expression and secretion of each cytokine by tumor necrosis factor-alpha(10 ng/ml) treatment, cultured human bronchial epithelial cell line BEAS-2B was added to each sample$(l,\;10,\;100\;&\;1000\;{\mu}g/ml)$. Subsequently, DNA activities were analyzed. Specifically mRNA expression and culture supernatants(protein levels) of IL-6 and GM-CSF from BEAS-2B cells, were analyzed using luciferase reporter gene assay, reverse transcription-polymerase chain reaction(RT-PCR) analysis and enzyme-linked immunosorbent assay. Results : Hirudo toasted with Ephedrae Herba(水蛭麻黃炒) and Hirudo(水蛭) inhibited IL-6 activities in BEAS-2B cells remarkably, and inhibited mRNA expression levels and protein levels in supernatant of IL-6 and GM-CSF at various concentrations, significantly(p<0.05). However, Hirudo toasted with Talcum(水蛭滑石炒) had no effect on mRNA expression levels and showed a slight inhibitory effect on GM-CSF protein levels in supernatant of culture medium. Conclusions : These results strongly suggest that Hirudo toasted with Ephedrae Herba(水蛭麻黃炒) and Hirudo(水蛭) would be serve as effective medicaments in the treatment of airway inflammation and remodeling of asthmatic patients.

• Toxicological Research
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• 제33권3호
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• pp.239-253
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• 2017

Neodymium is a future-oriented material due to its unique properties, and its use is increasing in various industrial fields worldwide. However, the toxicity caused by repeated exposure to this metal has not been studied in detail thus far. The present study was carried out to investigate the potential inhalation toxicity of nano-sized neodymium oxide ($Nd_2O_3$) following a 28-day repeated inhalation exposure in male Sprague-Dawley rats. Male rats were exposed to nano-sized $Nd_2O_3-containing$ aerosols via a nose-only inhalation system at doses of $0mg/m^3$, $0.5mg/m^3$, $2.5mg/m^3$, and $10mg/m^3$ for 6 hr/day, 5 days/week over a 28-day period, followed by a 28-day recovery period. During the experimental period, clinical signs, body weight, hematologic parameters, serum biochemical parameters, necropsy findings, organ weight, and histopathological findings were examined; neodymium distribution in the major organs and blood, bronchoalveolar lavage fluid (BALF), and oxidative stress in lung tissues were analyzed. Most of the neodymium was found to be deposited in lung tissues, showing a dose-dependent relationship. Infiltration of inflammatory cells and pulmonary alveolar proteinosis (PAP) were the main observations of lung histopathology. Infiltration of inflammatory cells was observed in the $2.5mg/m^3$ and higher dose treatment groups. PAP was observed in all treatment groups accompanied by an increase in lung weight, but was observed to a lesser extent in the $0.5mg/m^3$ treatment group. In BALF analysis, total cell counts, including macrophages and neutrophils, lactate dehydrogenase, albumin, interleukin-6, and tumor necrosis factor-alpha, increased significantly in all treatment groups. After a 4-week recovery period, these changes were generally reversed in the $0.5mg/m^3$ group, but were exacerbated in the $10mg/m^3$ group. The lowest-observed-adverse-effect concentration of nano-sized $Nd_2O_3$ was determined to be $0.5mg/m^3$, and the target organ was determined to be the lung, under the present experimental conditions in male rats.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 2002년도 창립10주년기념 및 국립독성연구원 의약품동등성평가부서 신설기념 국재학술대회:생물학적 동등성과 의약품 개발 전략을 위한 국제심포지움
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• pp.113-113
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• 2002

Phylogenetically conserved Bcl-2 family proteins play a pivotal role in the regulation of apoptosis from virus to human. Members of the Bcl-2 family consist of antiapoptotic proteins such as Bcl-2, Bcl-xL, and Bcl-w, and proapoptotic proteins such as BAD, Bax, BOD, and Bok. It has been proposed that anti- and proapoptotic Bcl-2 proteins regulate cell death by binding to each other and forming heterodimers. A delicate balance between anti- and proapoptotic Bcl-2 family members exists in each cell and the relative concentration of these two groups of proteins determines whether the cell survives or undergoes apoptosis. Mcl-1 (Myeloid cell :leukemia-1) is a member of the Bcl-2 family proteins and was originally cloned as a differentiation-induced early gene that was activated in the human myeloblastic leukemia cell line, ML-1 . Mcl-1 is expressed in a wide variety of tissues and cells including neoplastic ones. We recently identified a short splicing variant of Mcl-1 short (Mcl-IS) and designated the known Mcl-1 as Mcl-1 long (Mcl-lL). Mcl-lL protein exhibits antiapoptotic activity and possesses the BH (Bcl-2 homology) 1, BH2, BH3, and transmembrane (TM) domains found in related Bcl-2 proteins. In contrast, Mcl-1 S is a BH3 domain-only proapoptotic protein that heterodimerizes with Mcl-lL. Although both Mc1-lL and Mcl-lS proteins contain BH domains fecund in other Bcl-2 family proteins, they are distinguished by their unusually long N-terminal sequences containing PEST (proline, glutamic acid, serine, and threonine) motifs, four pairs of arginine residues, and alanine- and glycine-rich regions. In addition, the expression pattern of Mcl-1 protein is different from that of Bcl-2 suggesting a unique role (or Mcl-1 in apoptosis regulation. Tankyrasel (TRF1-interacting, ankyrin-related ADP-related polymerasel) was originally isolated based on its binding to TRF 1 (telomeric repeat binding factor-1) and contains the sterile alpha motif (SAM) module, 24 ankyrin (ANK) repeats, and the catalytic domain of poly(adenosine diphosphate-ribose) polymerase (PARP). Previous studies showed that tankyrasel promotes telomere elongation in human cells presumably by inhibiting TRFI though its poly(ADP-ribosyl)action by tankyrasel . In addition, tankyrasel poly(ADP-ribosyl)ates Insulin-responsive amino peptidase (IRAP), a resident protein of GLUT4 vesicles, and insulin stimulates the PARP activity of tankyrase1 through its phosphorylation by mitogen-activated protein kinase (MAPK). ADP-ribosylation is a posttranslational modification that usually results in a loss of protein activity presumably by enhancing protein turnover. However, little information is available regarding the physiological function(s) of tankyrase1 other than as a PARP enzyme. In the present study, we found tankyrasel as a specific-binding protein of Mcl-1 Overexpression of tankyrasel led to the inhibition of both the apoptotic activity of Mel-lS and the survival action of Mcl-lL in mammalian cells. Unlike other known tankyrasel-interacting proteins, tankyrasel did not poly(ADP-ribosyl)ate either of the Mcl-1 proteins despite its ability to decrease Mcl-1 proteins expression following coexpression. Therefore, this study provides a novel mechanism to regulate Mcl-1-modulated apoptosis in which tankyrasel downregulates the expression of Mcl-1 proteins without the involvement of its ADP-ribosylation activity.

• Biomolecules & Therapeutics
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• 제26권2호
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• pp.146-156
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• 2018

Spermidine is a naturally occurring polyamine compound that has recently emerged with anti-aging properties and suppresses inflammation and oxidation. However, its mechanisms of action on anti-inflammatory and antioxidant effects have not been fully elucidated. In this study, the potential of spermidine for reducing pro-inflammatory and oxidative effects in lipopolysaccharide (LPS)-stimulated macrophages and zebrafish was explored. Our data indicate that spermidine significantly inhibited the production of pro-inflammatory mediators such as nitric oxide (NO) and prostaglandin $E_2$ ($PGE_2$), and cytokines including tumor necrosis $factor-{\alpha}$ and $interleukin-1{\beta}$ in RAW 264.7 macrophages without any significant cytotoxicity. The protective effects of spermidine accompanied by a marked suppression in their regulatory gene expression at the transcription levels. Spermidine also attenuated the nuclear translocation of $NF-{\kappa}B$ p65 subunit and reduced LPS-induced intracellular accumulation of reactive oxygen species (ROS) in RAW 264.7 macrophages. Moreover, spermidine prevented the LPS-induced NO production and ROS accumulation in zebrafish larvae and was found to be associated with a diminished recruitment of neutrophils and macrophages. Although more work is needed to fully understand the critical role of spermidine on the inhibition of inflammation-associated migration of immune cells, our findings clearly demonstrate that spermidine may be a potential therapeutic intervention for the treatment of inflammatory and oxidative disorders.

• 패션비즈니스
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• 제8권2호
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• pp.42-55
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• 2004

The purpose of this study was to classify the contents of department stores and large-scale discount stores of consumer on information source, shopping orientation and store image in an effort to determine which variable gave a crucial impact on cosmetics department stores and large-scale discount stores selection behavior. The subjects of this study were 557 adult women visited department store and large-scale discount store in Busan. The data were analyzed by using Factor analysis, Frequency analysis, Correlation analysis, Cronabach $\alpha$ and Regression analysis. The results were as follows; 1. There was a difference in the demographical characteristics on department stores and large-scale discount stores of consumers. 2. Shopping Orientation of consumers were categorized into 5 types, and information source of consumers did 3 types, and store image of consumers did 5 types. 3. Leisure utilization, store & brand loyalty, store information, personal information, massmedia information, product & operate on, store atmosphere & salesperson and shopping convenience had positive correlations with cosmetics department stores selection beavior. but convenient location, rationality & economy and sales promotion had negative correlations with cosmetics department stores selection beavior. 4. Rationality & economy and sales promotion had positive correlations with cosmetics large-scale discount stores selection beavior. but convenient location, leisure utilization, store & brand loyalty, massmedia information, product & operate on, store atmosphere & salesperson and shopping convenience had negative correlations with cosmetics large-scale discount stores selection beavior. 5. Age, income, business(-), convenient location(-), rationality & economy(-), leisure utilization, store & brand loyalty, store information, personal information, massmedia information, store atmosphere & salesperson, shopping convenience and sales promotion(-) had a direct effect on cosmetics department stores selection beavior. Age, income, marriage, education had an indirect effect on department stores selection beavior through information source and store image, and information source did through store image, and shopping orientation did through store image. 6. Rationality & economy, convenient location(-), leisure utilization(-), store & brand loyalty(-), buying independence(-), personal information, massmedia information(-), product & operate on(-), shopping convenience(-) and sales promotion had a direct effect on cosmetics large-scale discount stores selection beavior. Age, income, marriage, education had an indirect effect on large-scale discount stores selection beavior through information source, shopping orientation and store image, and information source did through store image, and shopping orientation did through store image.

• 한국가정과교육학회지
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• 제26권2호
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• pp.15-30
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• 2014

본 연구는 중 고등학교의 청소년을 대상으로 그들의 의복행동과 관련하여 동조적 준거특성과 유명브랜드에 대한 태도에 따라 학교생활적응에 어떠한 차이가 있는지 파악해 봄으로써 학생들의 학교생활적응을 도울 수 있는 의생활지도와 가정 교과의 의생활 교육 영역과 관련된 기초자료를 제공하는 것을 목적으로 하였다. 그 결과를 요약하면 다음과 같다. 청소년의 유명브랜드에 대한 태도와 학교생활적응의 차이를 가져오는 일반적인 변수는 성적과 월 용돈으로 나타났으며 성적이 높고 월 용돈이 20만원이상이거나 5-10만원인 경우 유명브랜드를 더 선호하며 학교생활적응을 더 잘하는 것으로 나타났다. 소년의 의복행동동조 준거그룹은 부모동조, 친구동조, 매스미디어동조의 세 요인이었으며 유명브랜드 요인은 네임이미지선호 요인과 품질선호요인이었다. 부모동조요인이 친구나 매스미디어동조요인보다 상대적으로 높았지만 세 요인의 전체평균은 보통수준 이하였다. 의복행동 동조준거특성은 유명브랜드에 대한 태도의 요인에 비해 더 큰 차이를 가져왔으며 친구와 매스미디어의 동조경향이 강한 그룹이 부모동조의 경향이 높은 그룹보다 상대적으로 유명브랜드를 선호하였다. 의복행동의 동조준거에 따른 학교생활적응의 차이를 살펴보면 보통수준일 때 친구와 매스미디어의 동조가 상대적으로 높은 그룹이 낮은 그룹보다 학교생활적응경향이 높았다. 또한 부모동조경향이 상대적으로 가장 높은 그룹 또한 학교규율과 교우관계는 좋은 것으로 나타났다. 보편적인 수준에서 의복행동 동조준거 경향이 상대적으로 높은 경우 학교생활적응에 긍정적인 관계라고 할 수 있다. 청소년이 유명브랜드를 선호하는 경우 학교규율적응은 낮았고 교우관계와 교사와의 관계적응은 상대적으로 더 높았다. 학습적응에 있어서는 유명브랜드에 대한 태도에 따른 유의한 차이가 나타나지 않았다. 다시 말하면 청소년의 의복행동 동조는 극단적이지 않으며 보편적인 수준에서는 친구와 대중매체의 의복행동의 동조와 유명브랜드를 선호하는 태도가 학교생활 적응에 긍정적으로 작용한다고 볼 수 있다.

• 한국병원경영학회지
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• 제11권1호
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• pp.31-53
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• 2006

The objective of this study is to examine the effectiveness of the strategy of hospital specialization by analyzing the differences in expected and perceived services, perceived service quality, satisfaction, and intentions to revisit and recommend the hospital to others between general and specialty hospitals. Data were collected using self-administered questionnaire from patients admitted to four study hospitals: two speciality and two general hospitals. The questionnaire was developed based on SERVQUAL to measure five dimensions of service quality. Four hundreds questionnaires were distributed to inpatients or their guardians and 282 returned questionnaires were used in the analyses. The significance of the differences in study variables between specialty and general hospitals were tested by t-test and $x^2$-test. The factor analysis result confirmed the construct validity of 28 questions asked to measure service quality and resulted in four dimensions of service quality: reliability, assurance, tangible and empathy/responsiveness. Cronbach's Alpha ranged from .9013 to .9358, that confirmed the internal consistency of answers. The study results indicated that patients who used specialty hospitals had higher levels of expected and perceived service, a higher level of perceived service quality, and higher levels of service satisfaction than patients who used general hospitals. Percents of patients who had the intention to revisit the hospital and to recommend the hospital to others were higher among patients in specialty hospitals. The most frequent reason to choose the hospital was the excellence of doctors in both general(29.9%) and specialty(43.8%) hospitals, that was followed by convenient transportation(15.3%) and someone know works at the hospital(15.3%) in general hospitals and other's recommendation(14.6%), and nice amenities(13.1%) in specialty hospitals. Although there were no significant differences in clinical department, age, and sex of patients between general and specialty hospitals, patients who visited speciality hospitals had higher levels of education and income than their counter part in general hospitals. These results suggested that specialty hospitals performed better than general hospitals. Specialization could be a viable strategy to tide over recent financial difficulties experienced by hospitals, particularly small- and medium-sized hospitals.

• Journal of Periodontal and Implant Science
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• 제36권3호
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• pp.591-600
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• 2006

Purpose: This study assessed the impact of self-reported periodontal health on the oral health-related quality of life among elderly Koreans. Methods: Four hundred twenty one elderly Koreans in Seoul and suburban areas were selected with a cluster (institution) sampling method, and were requested to take oral examinations and finish questionnaires on the Oral Health Impact Profile-14(OHIP-14). and self-reported periodontal health status, such as periodontal symptoms, self-rated periodontal health and periodontal treatment need. As the dependent variable, OHIP-14 showed a positive skewed distribution (skewness: 1.17), we transformed to square-root form to apply parametric analyses. Bivariate analysis by t-test and ANOVA, and multivariate analysis with the two-level regression model accounting clusters were implemented. Results: Mean age of the subjects was 74.6 years and 66.5% were women. Fourteen items of OHIP-14 were summarized to one factor explaining 78.6% of total variance and produced the Chronbach alpha coefficient of 0.92. Results from the multivariate model, adjusting for age, sex, type of institutions, ability to pay, and number of teeth present, showed significantly lower OHIP-14 with reporting less than 3 periodontal symptoms (p(O.OOO1), rating their own periodontal health as above average level (p=O.0144), and thinking they don't need any periodontal treatments in the near future (p=O.0148), than their counterparts. The intraclass-corrrelation estimated by the final model was 0.028. Conclusion: This study demonstrates a significant association between self-reported periodontal health status and the oral health-related quality of life.