• Clinical and Experimental Pediatrics
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• v.49 no.10
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• pp.1093-1099
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• 2006

Purpose : Homocysteine is a strong and independent risk factor for cardiovascular disease. The deleterious effects of homocysteine included endothelial dysfunction, arterial intimal-medial thickening, wall stiffness and procoagulant activity. However, the precise mechanism responsible for homocysteine release in children with coronary artery disease is still unknown. The purpose of this study was to investigate serum homocysteine and tumor necrosis $factor(TNF)-{\alpha}$ levels and identify whether these levels had any association with the development of coronary artery lesions in Kawasaki disease(KD). Methods : Serum homocysteine and $TNF-{\alpha}$ levels were measured in 24 KD patients(group 1, eight patients with normal coronary artery; group 2, 16 patients with coronary artery lesions) and 21 controls(group 3, 10 afebrile controls; group 4, 11 febrile controls). Blood samples were drawn from each study group before and after intravenous immunoglobulin(IVIG) therapy and in the convalescent stage. Results : The homocysteine levels before IVIG therapy were significantly higher in group 1 than in group 3, and in group 2 than in group 3 and 4. The $TNF-{\alpha}$ levels before IVIG therapy were significantly higher in group 2 than group 3 and 4. Serum homocysteine and $TNF-{\alpha}$ levels were highest in group 2 before IVIG therapy. In the acute KD patients, serum homocysteine levels correlated significantly with $TNF-{\alpha}$ levels. Conclusion : The increased serum homocysteine levels in the acute stage increase the susceptibility to coronary arterial lesions in KD. $TNF-{\alpha}$ may also play an important role in the formation of coronary arterial lesions in KD.

• Tuberculosis and Respiratory Diseases
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• v.42 no.4
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• pp.492-501
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• 1995

Background: Transforming growth factor- alpha(TGF-$\alpha$) may play important roles in carcinogenesis, tumor growth, and angiogenesis. Transforming growth factor-beta(TGF-$\beta$) are known to be involved in cell-cycle control and regeneration. TGF-$\alpha$ positively acts on growth control of many epithelial cells in contrast to the negative role of TGF-$\beta$. Method: To evaluate the possible role of TGF-$\alpha$ and TGF-$\beta$ in human primary lung cancers, the expression of TGF-$\alpha$ and TGF-$\beta$ were immmunohistochemically investigated in tissue sections from forty seven cases with lung cancers and ten cases with non-cancerous lung tissues. Recombinant cloned monoclonal antibody of TGF-$\alpha$ and neutralizing antibody of TGF-$\beta$ were employed as primary antibodies after dewaxing the formalin-fixed, paraffinized tissue sections. Results: TGF-$\alpha$ was expressed in the cytoplasms of tumor cells in thirty five cases of forty seven(74.5%) primary lung cancers, whereas the control expressed in two of ten brochial epithelial cells. The expression of TGF-$\alpha$ was disclosed in four cases of eleven(36.4 %) small cell carcinomas and thirty one cases of thirty six(86.1%) non-small cell carcinomas of the lung. Expressions of TGF-$\beta$ was discernible in bronchial epithelium in eight of ten non-cancerous lung tissues. The expression of TGF-$\beta$ was noted in the cytoplasms of tumor cells in eight cases of forty seven(17.0%) primary lung cancers. The expression of TGF-$\beta$ disclosed in two cases of eleven(18.2%) small cell carcinomas and six cases of thirty six(16.7%) non- small cell carcinomas of the lung. Conclusion: These findings suggest that up-regulation of TGF-$\alpha$ and down-regulation of TGF-$\beta$ are involved during development and growth of primary lung cancers.

• Proceedings of the Korean Society for Food Science of Animal Resources Conference
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• 2000.06a
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• pp.98-98
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• 2000

• Biomedical Science Letters
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• v.21 no.2
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• pp.126-130
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• 2015

There are several studies that show hypothermia improves cellular ischemia damages on experimental and clinical bases. However, its exact molecular mechanisms are unclear. In this study, we demonstrate that hypothermia induced insulin-like growth factor 1 (IGF1) gene expression, and its expression was dramatically decreased under ischemic insults. It was also demonstrated that hypothermia activated endoplasmic reticulum (ER) stress sensors especially both the phosphorylation of $eIF2{\alpha}$ (eukaryotic translation initiation factor 2 alpha) and ATF6 (activating transcription factor-6) proteolytic cleavage. However, the factors of apoptosis and autophagy were not associated with hypothermia. We suggest that hypothermia-treated IGF1 gene expression after ischemia may show a good possibility for the development of treatments and diagnostic methods in cerebral ischemic damages.

• Membrane Journal
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• v.14 no.4
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• pp.298-303
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• 2004

PTMSP/PDMS-PEI composite membrane was prepared by solution casting method. To investigate the characteristics of this membrane, the analytical methods such as FT-IR, $^1$H-NMR, DSC, TGA, GPC, and SEM have been utilized. The number-average((equation omitted)) and weight-average((equation omitted)) molecular weight of PTMSP/PDMS copolymer were 501,516 and 675,560 respectively. The separation of the gas mixture($H_2$/$N_2$) through the composite membrane was studied as a function of pressure. The separation factor($\alpha$, $\beta$, (equation omitted)) of the composite membrane used in this work increased as the pressure of permeation cell increased. The real separation factor($\alpha$), head separation factor($\beta$), and tail separation factor ((equation omitted)) of PTMSP/PDMS-PEI composite membrane were 21.50, 49.14 and 1.84 respectively at $\Delta$P 345.55 kPa and $25^{\circ}C$.

• Korean Journal of Adult Nursing
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• v.28 no.2
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• pp.191-201
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• 2016

Purpose: The purpose of this methodological study was to examine the validity and reliability of a translated Korean version of The Oncology Patients' Perception of the Quality of Nursing Care Scale. Methods: The translated scale was pilot tested and then administered to 360 patients with cancer. Exploratory and confirmatory factor analyses were utilized to assess the factor structure. Internal consistency reliability was determined using coefficient ${\alpha}$. Results: Two of the 40 items were deleted with a principal component method of exploratory factor analysis because they did not meet the factor-loading criterion. The 38 items were again analyzed and, four factors were validated (KMO=.96, Bartlett ${\chi}^2=10809.81$, df=780, p<.001). The four factors accounted for 60.9% of the variance. Factor loadings of the scale on the four subscales ranged from .65 to .91. The scale showed reliable internal consistency with a Cronbach's ${\alpha}$, total 38 items (${\alpha}=.96$) in four subscales: individualization (18 items, ${\alpha}=.96$), proficiency (10, .91), responsiveness (7, .90), and coordination (3, .80). Conclusion: The findings of this study demonstrate that the scale has satisfactory construct validity and reliability to measure quality of cancer nursing care from the patient's perspective in Korea.

• Journal of Korean society of Dental Hygiene
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• v.12 no.4
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• pp.675-684
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• 2012

Objectives : The purpose of this study was to develop an instrument for Korean Scaling Fear (KSF)-1.1 in scaling patients. Methods : 402 sample size for scaling patients was studied in Daegu city in July and August of 2011. Mean and standard deviation was calculated in 3 dimensions(FWS: fear while scaling, DDH: distrust on dental hygienist, FAS: fear after scaling). Results : Age of 402 subjects was 36.5 years. In analyzing reliability for item-level, a range of correlation coefficient(${\alpha}$) on item-internal consistency(FWS, DDH, and FAS) was 0.58~0.88(${\alpha}$=0.90), 0.40~0.71(${\alpha}$=0.82), and 0.54~0.63(${\alpha}$=0.82), respectively. Floor(%) and ceiling(%) value on 3 dimensions were also 9.2% and 4.0%, 12.4% and 0.5%, and 17.7% and 1.2%, respectively, therefore, we found statistically high reliability for those(p<0.001). With explanatory factor analysis, this study could generate 3 dimensions(factor 1, eigenvalue 5.41, proportion 0.49; factor 2, eigenvalue 1.50, proportion 0.14; factor 3, eigenvalue 1.04, proportion 0.09) and 11 sub-scales. Also confirmatory factor analysis results showed that the KSF1.1 model was fitted very well in analysis of model fit($x^2$=112.94, df=41, p=0.000; goodness of fit index=0.95; adjusted goodness of fit index=0.92; root mean square residual=0.057). Conclusions : In conclusion, The findings of this study showed that developed reliable and valid instrument for measuring the KSF1.1 in the scaling patients.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.1
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• pp.508-517
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• 2016

The aim of this study was to test the validity and reliability of the Suicide Resilience Inventory-Korean version. Data were collected from 266 university students in Jeonbuk, South Korea. The Suicide Resilience Inventory-Korean version Suicide Resilience Inventory-25 was developed, and the collected data were analyzed using confirmatory factor analysis and Cronbach's alpha. Confirmatory factor analysis showed that The Suicide Resilience Inventory-Korean version(SRI-K) had factor loadings of the 19 items on the three subscales from 0.442 to 0.767. The three subscales model was validated by confirmatory factor analysis (RMSEA<.08, $CFI{\geq}.9$). The internal consistency with a Cronbach's alpha was reliable with a total scale of 0.943. These findings show that the SRI-K has satisfactory construct validity and reliability to measure the ability to effectively overcome and to flexibly cope with the suicide risk.

• Microbiology and Biotechnology Letters
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• v.36 no.1
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• pp.49-54
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• 2008

The gene encoding human pancreatic pro-carboxypeptidase B (CPB) was cloned and fused to Saccharomyces cerevisiae mating factor alpha-1 secretion signal $(MF{\alpha}1)$, in which the transcription of $MF{\alpha}1$-pro-CPB was under the control of GAL10 promoter. The constructed plasmid $pY{\alpha}$-hproCPB(7.72 kb) was transformed into S. cerevisiae 2805. The recombinant human pro-CPB (hproCPB) was successfully expressed in S. cerevisiae after induction of galactose, and could be secreted into the culture medium. By analyses of SDS-PAGE and western blotting, the molecular weight of the purified hproCPB was estimated to be a 45.9kDa. The activity of extracellular hCPB after removal of pro-region by trypsin treatment reached about 10.16 unit/ml at batch culture of S. cerevisiae $2805/pY{\alpha}$-hproCPB for 60 h. Also, the Km value of partially purified recombinant hCPB is about 0.43 mM.

• The Korean Journal of Physiology and Pharmacology
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• v.16 no.2
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• pp.131-138
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• 2012

Alcoholic hepatitis is a leading cause of liver failure in which the increased production of tumor necrosis factor ${\alpha}$ (TNF${\alpha}$) plays a critical role in progression of alcoholic liver disease. In the present study, we investigated the effects of cilostazol, a selective inhibitor of type III phosphodiesterase on ethanol-mediated TNF${\alpha}$ production in vitro and $in$ $vivo$, and the effect of cilostazol was compared with that of pentoxifylline, which is currently used in clinical trial. RAW264.7 murine macrophages were pretreated with ethanol in the presence or absence of cilostazol then, stimulated with lipopolysacchride (LPS). Cilostazol significantly suppressed the level of LPS-stimulated TNF${\alpha}$ mRNA and protein with a similar degree to that by pentoxifylline. Cilostazol increased the basal AMP- activated protein kinase (AMPK) activity as well as normalized the decreased AMPK by LPS. AICAR, an AMPK activator and db-cAMP also significantly decreased TNF${\alpha}$ production in RAW264.7 cells, but cilostazol did not affect the levels of intracellular cAMP and reactive oxygen species (ROS) production. The $in$ $vivo$ effect of cilostazol was examined using ethanol binge drinking (6 g/kg) mice model. TNF${\alpha}$ mRNA and protein decreased in liver from ethanol gavaged mice compared to that from control mice. Pretreatment of mice with cilostazol or pentoxifylline further reduced the TNF${\alpha}$ production in liver. These results demonstrated that cilostazol effectively decrease the ethanol-mediated TNF${\alpha}$ production both in murine macrophage and in liver from binge drinking mice and AMPK may be responsible for the inhibition of TNF${\alpha}$ production by cilostazol.