• 제목/요약/키워드: ${\alpha}+{\beta}$ phase

검색결과 532건 처리시간 0.019초

Bleomycin Inhibits Proliferation via Schlafen-Mediated Cell Cycle Arrest in Mouse Alveolar Epithelial Cells

  • Jang, Soojin;Ryu, Se Min;Lee, Jooyeon;Lee, Hanbyeol;Hong, Seok-Ho;Ha, Kwon-Soo;Park, Won Sun;Han, Eun-Taek;Yang, Se-Ran
    • Tuberculosis and Respiratory Diseases
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    • 제82권2호
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    • pp.133-142
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    • 2019
  • Background: Idiopathic pulmonary fibrosis involves irreversible alveolar destruction. Although alveolar epithelial type II cells are key functional participants within the lung parenchyma, how epithelial cells are affected upon bleomycin (BLM) exposure remains unknown. In this study, we determined whether BLM could induce cell cycle arrest via regulation of Schlafen (SLFN) family genes, a group of cell cycle regulators known to mediate growth-inhibitory responses and apoptosis in alveolar epithelial type II cells. Methods: Mouse AE II cell line MLE-12 were exposed to $1-10{\mu}g/mL$ BLM and $0.01-100{\mu}M$ baicalein (Bai), a G1/G2 cell cycle inhibitor, for 24 hours. Cell viability and levels of pro-inflammatory cytokines were analyzed by MTT and enzyme-linked immunosorbent assay, respectively. Apoptosis-related gene expression was evaluated by quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR). Cellular morphology was determined after DAPI and Hoechst 33258 staining. To verify cell cycle arrest, propidium iodide (PI) staining was performed for MLE-12 after exposure to BLM. Results: BLM decreased the proliferation of MLE-12 cells. However, it significantly increased expression levels of interleukin 6, tumor necrosis factor ${\alpha}$, and transforming growth factor ${\beta}1$. Based on Hoechst 33258 staining, BLM induced condensation of nuclear and fragmentation. Based on DAPI and PI staining, BLM significantly increased the size of nuclei and induced G2/M phase cell cycle arrest. Results of qRT-PCR analysis revealed that BLM increased mRNA levels of BAX but decreased those of Bcl2. In addition, BLM/Bai increased mRNA levels of p53, p21, SLFN1, 2, 4 of Schlafen family. Conclusion: BLM exposure affects pulmonary epithelial type II cells, resulting in decreased proliferation possibly through apoptotic and cell cycle arrest associated signaling.

아까시나무(Robinia pseudoacacia L.)와 분홍아까시나무(R. margarettae 'Pink Cascade') 향기성분 조성 비교 (Comparative Study of Floral Volatile Components in the Different Species of Robinia spp.)

  • 이수진;김영기;노광래;이현숙;김문섭;김세현;권형욱
    • 한국양봉학회지
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    • 제34권3호
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    • pp.189-195
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    • 2019
  • 본 연구는 아까시나무 꽃으로부터 발산되는 휘발성 성분을 SPME법으로 추출하여 GC/MS를 통해 분석을 시도하여, 서로 다른 수종 별로 발산하는 FVCs의 성분과 그 구성비율이 확연하게 차이가 있음을 알 수 있었다. 아까시나무, 4배체 아까시나무, 분홍아까시나무에서 FVCs 분석결과를 비교해 볼 때 차이를 보이는 주요 FVCs는 각각 Linalool (35.47%), α-Farnesene (33.94%), (E)-4,8-Dimethylnona-1,3,7-triene (37.23%)으로 나타났으며, 공통적으로 (Z)-β-ocimene이 30% 이상을 차지하고 있음을 확인하였다. 이에 따라 수종 간 FVCs 조성비의 차이가 꿀벌의 방화 행동 유도에 중요한 역할을 할 것으로 추측되며, 아까시나무의 FVCs에 대한 꿀벌의 선호도 및 방화 행동의 연관성에 대해 심층적인 연구가 수행되어야 할 것으로 보인다.