• 한국식생활문화학회지
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• 제19권6호
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• pp.598-604
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• 2004

This was aim to study the changes of components by different parts and maturity of tomato. We found that orally fed tomatine which induced a significant reduction in cholesterol in hamster in contrast to potato glycoalkaloids, the safety of tomatine was supported by our observation that pickled green tomatoes consumed widely in many countries has a high tomatine content. The tomatoes harvested during the first stage(10 days after flowering) contained tomatine $6333.49{\mu}g\;per\;100g$ and dohydrotomatine and ${\alpha}$-tomatine in a ratio of about 1:13.8. The tomatine content then decreased by about 85% during stage 2(20 days after flowering) an dehydrotomatine contene dropped to a value near zero during stage 3, 4 It on the tomatine content dropped to a value near zero during the final stage(50 days after flowering). With respect to health benefits at all stages of maturity, unripe tomato contain chlorophyll and ${\alpha}$-tomatine should consumed.

• 한국식품영양학회지
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• 제23권4호
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• pp.556-561
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• 2010

Tomato fruits(Lycoperisicon esculentum) synthesize the glycoalkaloids dehydrotomatine and ${\alpha}$-tomatine, possibly as defense against bacteria, fungi and insects. We developed a new effective method to prepare and purify dehydrotomatine and ${\alpha}$-tomatine that exists in tomato fruits using alumina column chromatography and high performance liquid chromatography (HPLC). The tomato glycoalkaloids(TGA) in tomato was extracted with 2% acetic acid, and then precipitated with ammonium hydroxide(pH=10.5). The dry precipitate substance was applied on alumina column, and then fractionated with water saturated n-butylalcohol. The TGA(Fr. No. 26~36) were collected and dried under reduced pressure. The TGA was performed on a reverse phase HPLC(Inertsil ODS-2, $5\;{\mu}m$), eluted with acetonitrile/20mM $KH_2PO_4$(24:76, v/v) at 208 nm. Two peaks were detected on HPLC, and individual peak was collected by repeating HPLC. Furthermore, to confirm the identity dehydrotomatine and ${\alpha}$-tomatine, each peak isolated was hydrolyzed with 1N HCl into sugar and aglycone tomatidine. The sugars were converted to trimethylsilyl ester derivatives. The nature and molar ratios of sugars were identified by gas-liquid chromatography(GLC) and the aglycone by high-performance liquid chromatography(HPLC). The first peak (Rt=17.5 min) eluted from HPLC was identified as dehydrotomatine, and second peak(Rt=21.0 min) was as ${\alpha}$-tomatine. This technique has been used effectively to prepare and isolate dehydrotomatine and ${\alpha}$-tomatine from tomato fruits.

• 원예과학기술지
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• 제32권2호
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• pp.252-260
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• 2014

Contents of carotenoids, phenolic compounds, volatile organic compounds, and alkaloids in leaves, internodes, fruits, and roots of tomatoes in different developmental stages were measured. Lycopene, ${\beta}$-carotene, and lutein were detected in all the tested parts except roots and green fruits. Lycopene content in red fruits was $49.04{\mu}g{\cdot}g^{-1}$ FW, while that in the other parts was below $40{\mu}g{\cdot}g^{-1}$ FW. ${\beta}$-Carotene and lutein contents in 24th leaves were 5.81 and $6.40{\mu}g{\cdot}g^{-1}$ FW, respectively, and were greater than those in the other parts. Caffeic, chlorogenic, and vanillic acids were detected in all the tested parts except roots. The content of chlorogenic acid in the 18th leaves was $40.11{\mu}g{\cdot}g^{-1}$ FW, while that in the other parts was lower than $31.00{\mu}g{\cdot}g^{-1}$ FW. The contents of caffeic and vanillic acids in the 24th leaves were 9.18 and $1.64{\mu}g{\cdot}g^{-1}$ FW, respectively, and were greater than those in the other parts. Moreover, younger leaves contained the more diverse volatile organic compounds including monoterpenes and sesquiterpenes. Contents of dehydro-tomatine and ${\alpha}$-tomatine were greatest in leaves, followed by internodes, roots and fruits. Younger leaves and internodes contained more dehydro-tomatine and ${\alpha}$-tomatine than older leaves and internodes. The contents of dehydro-tomatine and ${\alpha}$-tomatine in the 24th leaves were 0.89 and $1.42mg{\cdot}g^{-1}$ FW, respectively, and were greatest among all the tested parts. Our results indicated that, except lycopene, tomato leaves included greater secondary metabolites contents than red fruits. The results suggest that inedible parts of tomato plants can be used as raw material for antioxidants, anti-inflammatory agents, fungistats, and pesticides.

• 한국응용곤충학회지
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• 제39권3호
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• pp.157-163
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• 2000

청동방아벌레((Selatosomus puncticollis Motschulsky) 유충에 의한 50종 감자(Solanum tuberosum L.) 품종의 피해정도를 검정한 전보의 결과를 바탕으로, 10개 품종(수미, 조풍, 남작, 대서, 대지, 세풍, 남서, Anco, Bintje, Denali)의 괴경피해율과 피해도를 포장과 실내에서 검정하였다. 또한 품종별 피해도 차이에 대한 원인을 구명하고자 괴경에 함유된 여러 가지 성분들(glycoalkaloids, 당, 전분, 전질소, Ca, K, Mg)의 함량을 측정하였다. 청동방아벌레 유충에 대한 10개 품종의 포장 피해괴경율은 19~72%로 높았다. 실내검정 결과 청동방아벌레 유충에 의한 피해구멍수는 남서 품종에서 많았으나, 수미, 대서, Anco, Bintje, Denali 품종은 상대적으로 적었다. 또한 방아벌레 유충에 대한 $\alpha$-chaconine, $\alpha$-solanine, $\alpha$-tomatine의 활성을 실내에서 검정한 결과, 2,500 ppm 농도에서 어떠한 활성도 관찰되지 않았다. 괴경의 glycoalkaloids 함량은 품종별로 차이가 많았는데, 수미품종은 18.83 mg%로 가장 많이 함유하였으나, 남작 품종은 6.39%로 가장 적었다. 괴경중 화원당과 총당 함량은 남서 품종에서 각각 0.71%와 2.95%였으나, 대지 품종은 0.26%와 1.77%였다. 전분 함량은 대지, 남작, Bintje 품종이 많았던 반면 조풍과 세풍 품종에서는 적었다. 괴경중 전질소, K를 가장 많이 함유한 품종은 Anco 품종이었고, Ca는 대서 품종에 Mg는 조풍 품종에 가장 많았다. 이러한 결과를 바탕으로 품종별 해충의 피해도와 저항성 관련 요인들간의 상관성을 분석한 결과, 청동방아벌레 유충에 의한 피해는 괴경중 전질소 함량과는 부의 상과(r=-0.71435*)이었으나, 괴경중 총당 함량과는 정의 상관(r=0.78018*)을 보였다. 이러한 결과는 해충종합방제 프로그램을 확립하고 또한 새로운 내충성 품종의 육성에 기초자료가 될 것으로 생각된다.