• 한국약용작물학회지
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• 제8권4호
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• pp.342-350
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• 2000

천연물유래의 안전하고 효과적인 항산화 화합물을 찾기 위하여 채취한 86종의 다양한 식물체로부터 얻은 MeOH 추출물을 DPPH free radical 소거법을 이용하여 항산화활성을 검정한 결과 밤껍질 $(RC_{50}=5.8{\mu}g)$과 느릅나무 $(RC_{50}=12{\mu}g)$에서 대조구인 ${\alpha}-tocopherol(RC_{50}=12{\mu}g)$ 과 BHA$(RC_{50}=14{\mu}g)$에 비해 강한 활성을 보였고 그외 13종에서 도 비교적 강한 활성을 나타내었다. 또한 대표적인 식물병원균 중 6종의 균주를 사용하여 in vivo 방법으로 항미생물 활성을 검정한 결과 굴피나무, 비목나무, 산국, 소리쟁이, 가막사리, 아왜나무에서 90% 이상의 식물병원균의 생육저해 효과를 보였다.

• Preventive Nutrition and Food Science
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• 제18권3호
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• pp.188-195
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• 2013

Antioxidants are an important group of medicinal preventive compounds as well as being food additives inhibiting detrimental changes of easily oxidizable nutrients. The present investigation has been carried out to evaluate the antioxidant properties of different solvent extracts of Agriophyllum pungens seeds by various in vitro systems. The antioxidative activities of these samples were determined using four methods: 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'- azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) radical scavenging activity, ferric-reducing antioxidant potential (FRAP), and hydroxyl (OH) radical scavenging activities. Additionally, total flavonoids and phenolic contents (TPC) were also determined. Yield of extracts varied widely among solvents and was the highest for water extract (5.642% based on dry weight basis), while ethyl acetate extract exhibited the highest total phenolic content (0.149 mg/mL), total flavonoid content (0.111 mg/mL), and antioxidant activities (P<0.05). The ABTS radical scavenging activity of A. pungens seeds occurred in the following order: ascorbic acid (92.9157%)>BHA (90.1503%)> ${\alpha}$-tocopherol (87.7527%)>APEA (83.9887%) >APWR (75.5633%); the antioxidant activity of the extracts might be attributed to the presence of these phenolics. This suggests that A. pungens seed extract is a potential source of natural antioxidants, which could be added to dietary supplements to help prevent oxidative stress.

• Applied Biological Chemistry
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• 제47권2호
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• pp.283-286
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• 2004

In the course of screening for reactive oxygen species scavengers from natural products, two antioxidants were isolated from the edible mushroom Polyozellus multiflex and identified as polyozellin and thelephoric acid. Thelephoric acid inhibited 45.7% of the super oxide anion radical, 74.6% of the hydroxyl radical, and 44.0% of the DPPH radical at 0.1 mM, while the positive control ${\alpha}-tocopherol$ did 22.1%, 75.6%, and 26.5% of each radical, respectively. Polyozellin, the reductive acetylated form of thelephoric acid, showed almost same scavenging activity against above mentioned radicals. The isolated compounds showed scavenging activity on the superoxide anion radical in the ESR method ($IC_{50}$ of polyozellin and thelephoric acid were $218.0\;{\mu}M$ and $21.1\;{\mu}M$, respenctively). However, they showed no significant activity on the hydrogen peroxide radical.

• 생약학회지
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• 제28권4호
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• pp.198-208
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• 1997

This Study was attempted to investigate the effect of Allium cepa L. (onion) on the activities of GOT and GPT, the levels total lipids, triglyceride, total cholesterol, phospholipid and ${\beta}-lipoprotein$ in the serum and the increase ratio of body and liver weight in the experimentally induced hyperlipemic rats. The activities of S-GOT and S-GPT were significantly decreased in dose of onion juice 3 ml and 5 ml/head as compared to the high lipid-diet control group. The levels of total lipids. Triglyceride, total cholesterol and phospholipids were significantly decreased in dose of onion juice 3 ml and 5 ml/head as compared to the control group, respectively. However, the level of ${\beta}-lipoprotein$ was significantly decreased in dose of onion juice 3 ml/head. The levels of triglyceride and total cholesterol in the liver were significanatly decreased in dose of onion juice 3 ml/head. Increase ratio of the body and liver weight were significantly decreased in dose of onion juice 3 ml/head. Anti-fatigue activity of onion juice were studied in mice using the swimming performance method. The potencies of anti-fatigue acti-vities was significantly found in 1.2 ml/head. p.o. of onion juice. The methanol extract and juice extract of onion was significantly found to have an antioxidative activity on the air oxidation of linoleic acid as compared to the 3-butyl-4-hydroxyanisole and ${\alpha}-tocopherol$.

• Journal of Nutrition and Health
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• 제33권5호
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• pp.507-516
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• 2000

The purpose of this study was to investigate the effect of vitamin C and vitamin E supplementation on the iron contents and oxidative stress of the rats. Rats were fed 18g ascorbic acid and 300IU $\alpha$-tocopherol/kg diet, respectively. Rats were sacrificed at 1, 3, 5 and 7 month of age. The blood, liver and brain were selected for the quantitation of iron and malondialdehyde(MDA) contents, glutathione peroxidase(GSHPx), superoxided dismutase(SOD) and catalase(CAT) activity. Iron and MDA contents and GSHPx activities were increased with aging. Vitamin C and Vitamin E supplementation increased iron contents of the plasma. Vitamin C raised iron contents, but vitamin E decreased iron contents of the liver. In the brain vitamin C and vitamin E did not affect the iron level. MDA levels were decreased with vitamin C and vitamin E supplementation in the erythrocyte and liver, and vitamin C supplementation elevated MDA levels in the brain. GSHPx activity was increased with vitamin C and vitamin E supplementation. SOD activities of erythroucyte and brain were not affected with age, but in the liver, SOD activity was raised with age and vitamin C supplementation. Vitamin C and vitamin E supplementation promoted CAT activity of erythroucyte and liver, and CAT activity of brain was eleveated with vitamin addition but was decreaed with vitamin E addition. Vitamin C and vitamin E decreased iron contents of blood plasma, MDA contents of plasma and liver, and CAT activity of erythrocyte. Above results indicated that iron contents and biomarkers of oxidative stress were more affected by age than antioxidant action of vitamin C and vitamin E.

• Journal of Nutrition and Health
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• 제46권2호
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• pp.126-136
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• 2013

This study was conducted in order to investigate the association between hypertension and oxidative stress-related parameters and to evaluate these parameters in subclinical hypertensive patients and normotensive subjects living in Korea. We attempted to determine whether oxidative stress-related parameters would differ between two groups of 227 newly-diagnosed, untreated (systolic blood pressure (BP) ${\geq}$ 130 mmHg and diastolic BP ${\geq}$ 85 mmHg) and 130 normotensive subjects (systolic BP < 120 mmHg and diastolic BP < 80 mmHg). General characteristics of the subjects were collected using a simple questionnaire. From subjects' blood, degree of DNA damage in lymphocytes, the activities of erythrocyte superoxide dismutase, catalase, and glutathione peroxidase, level of plasma total radical-trapping antioxidant potential (TRAP), glutathione, and anti-oxidative vitamins, as well as plasma lipid profiles and conjugated diene (CD) were analyzed. Evaluation of the associations of oxidative stress-related parameters with blood pressure of the subjects was performed using Pearson partial correlation and multivariate logistic regression analysis after adjusting for confounding factors. Several oxidative stress-related parameters were higher in subclinical hypertensive patients than in normotensive subjects. Plasma levels of ${\alpha}$-tocopherol, ${\beta}$-carotene, TRAP, and activity of GSH-px were significantly lower in subclinical hypertensive patients than in normotensive subjects. Increased levels of DNA damage, lipid peroxidation, triglyceride, total cholesterol, and LDL-cholesterol were observed in subclinical hypertensive patients. These results confirm an association between blood pressure and oxidative stress-related parameters and suggest that the pathogenic role of oxidative stress in hypertension might be significant.

• Journal of Nutrition and Health
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• 제41권4호
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• pp.291-298
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• 2008

To study antioxidant role of zinc, the effects of dietary zinc deficiency and vitamin E supplementation on lipid peroxidation were studied. Levels of zinc and vitamin E in blood and liver were also measured. Forty Sprague-Dawley male rats aging 8 weeks old were used as experimental animals. Zinc deficient diet (Zn, 0 ppm), zinc normal diet (Zn,36.5 ppm), and vitamin E supplemented diet (1,000 IU ${\alpha}$-tocopherol/kg of diet) were used as experimental diet. During the first three weeks, rats were divided into zinc normal (ZnN, 8 animals) and zinc deficient (ZnD, 32 animals) group. Eight rats from each group were sacrificed to get blood and liver after 3 weeks of experiment. The remaining 24 zinc deficient rat were then divided into zinc normal (ZnDN), zinc deficient (ZnDD), vitamin E supplemented (ZnDE) diet groups. After another 3 weeks of experiment, all animals were sacrificed as well. Thiobarbituric acid reactive substanc (TBARS) levels in plasma and liver, conjugated diene levels in liver were measured as lipid peroxidation index. There were no significant differences in food intake, body weight gain, and food efficiency ratio among groups. Weights of liver per 100 g body weight were not significantly different. There were no significant differences in Zn levels in serum. Plasma and liver TBARS level, and liver conjugated diene level were significantly lower in ZnDE than in ZnDN or ZnDD, and significantly higher in ZnDD than in ZnDN. Therefore, it seems that lipid peroxidation is accelerated by dietary zinc deficiency and recovered partly by vitamin E supplementation.

• 한국축산식품학회지
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• 제39권4호
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• pp.632-642
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• 2019

The concentrations of functional compounds and micronutrients of chicken breast from native chickens were compared with those from broiler. Totally 200 male chicks from a commercial native chicken (HH) and three newly bred native chicken strains (2A, 2C, and 2D) were reared for about 2 kg of final live weight up to 12 wk. After slaughtered, antioxidant dipeptides, reducing sugar, free amino acids, vitamins, and minerals of the breast muscles were analyzed with those from broilers with similar live weight. Mostly native chicken strains had higher contents of carnosine, anserine, and reducing sugar than the broiler. Especially HH implied the highest values of carnosine and anserine, and 2C did the highest of reducing sugar (p<0.05). Vitamin A contents between native chickens and broiler were not significantly different (p>0.05). The contents of ${\alpha}-tocopherol$ were significantly higher in 2C than those of HH or broiler (p<0.05). Native chicken strains contained lower cholesterol levels than the broiler. Broiler had higher contents of P, Mg, and Na than native chickens (p<0.05), but it had lower content of Cu than HH or 2A. The savory free amino acids including glutamic acid was highest in 2A than the other native chickens and broiler (p<0.05). This study confirms that certain new strains of native chickens be a good source in terms of functional compounds and micronutrients which can be attractive health promoting nutritional quality factors.

• KSBB Journal
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• 제23권4호
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• pp.329-334
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• 2008

오가피는 건강보조식품, 식품이나 화장품 등의 첨가제로 좋은 약재로 평가되고 있다. 그러나 오가피의 연구의 대부분은 가온 용매추출이나 열수추출을 통한 유효성분의 추출과 생리활성 연구에 치중되어 있다. 따라서 온도 및 추출용매를 조절하여 추출하고 Diaion HP2O 분획을 통한 유효성분의 함량 및 항산화활성에 대하여 연구하였다. 오가피추출물은 열에 불안정하여 갈변현상을 보이므로 상온추출 및 냉온추출하는 것of 좋으며, 에탄올 함량별로 추출시 주 활성성분인 chlorogenic acid의 함량을 보면 100% 정제수 및 100% 에탄올의 단일 용매 추출보다 에탄올수용액을 사용하여 추출시 chlorogenic acid의 함량이 증가함을 알 수 있었다. 에탄올수용액에서의 chlorogenic acid의 함량은 유사하므로 농축시간을 고려하여 70$\sim$85% 에탄올수용액으로 추출하는 것이 바람직할 것으로 사료된다. 최적화된 조건에서 추출된 오가피추출물은 Diaion HP2O column chromatography를 이용하여 10%, 20%, 40%, 60% 에탄올수용액으로 분획 후, 각 분획층의 주 활성성분 합량과 항산화효과를 확인하였다. 10% 및 20% 에탄올 수용액층은 chlorogenic acid가 주 구성성분이었으며, 40% 에탄올 수용액층은 caffeic acid 및 eleutheroside E가 주 구성성분임을 알 수 있었다. Chlorogenic acid와 caffeic acid 함량이 높은 20% 및 40% 에탄올수용액 분획층에서 우수한 DPPH와 superoxide radical에 대한 소거효과를 나타내었다. DPPH radical 소거효과에서 20% 및 40% 에탄올수용액 분획층은 각각 $IC_{50}$ 값이 $1.748{\pm}0.098{\mu}g/ml$, $11.487{\pm}1.768{\mu}g/ml$을 나타내었으며, Superoxide radical 소거효과에서는 $131.491{\pm}2.235{\mu}g/ml$, $200.681{\pm}2.444{\mu}g/ml$로 양성 대조군으로 사용한 ascorbic acid와 동등하거나 dl-$\alpha$-tocopherol보다 우수한 항산화효과를 보였다. 각 분획층의 항산화효과를 미루어 볼 때, 20% 및 40%의 주 구성성분을 조합하여 오가피추출물을 제조하면 더욱 우수한 소재로 개발할 수 있을 것으로 사료된다. 본 기술연구소에서는 최적화된 조건에서 추출된 오가피추출물 및 Diaion HP2O 분획물을 이용하여 B16 melanoma 세포에서의 멜라닌 생성저해 효과를 연구하여 새로운 미백제를 개발 중에 있으며, 건강보조식품, 기능성 향장품소재 등으로의 활용 가치가 높을 것으로 사료된다.

• 대한화장품학회지
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• 제37권4호
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• pp.357-363
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• 2011

본 연구에서는 대황 추출물의 항산화 활성, 타이로시네이즈(tyrosinase) 저해 활성을 확인하였다. 대황의 50 %에탄올 추출물, 에틸아세테이트(ethyl acetate) 분획, 아글리콘(aglycone) 분획으로 실험을 진행하였다. 대황 추출물들의 DPPH (1,1-diphenyl-2-picrylhydrazyl) 소거활성($FSC_{50}$)은 대표적인 항산화제인 (+)-${\alpha}$-tocopherol보다 낮은 것으로 나타났다. Luminol 발광법을 이용한 $Fe^{3+}$-EDTA/$H_2O_2$ 계에서 생성된 활성산소종에 대한 아글리콘 분획의 소거활성(총 항산화능, $OSC_{50}$)은 $0.265\;{\mu}g/mL$로 매우 큰 활성을 나타내었다. 대황 추출물의 rose-bengal로 증감된 $^1O_2$에 의한 적혈구 파괴에 대한 세포보호 효과는 모든 분획에서 농도 의존적($1{\sim}50\;{\mu}g/mL$)으로 증가하였으며, 특히 아글리콘 분획은 $10\;{\mu}g/mL$ 농도에서 ${\tau}_{50}$이 757.0 min으로 높은 세포 보호 활성을 나타내었다. 대황 추출물 중 아글리콘 분획의 타이로시네이즈 저해활성($IC_{50}$)은 $11.20\;{\mu}g/mL$으로 $226.88\;{\mu}g/mL$인 알부틴(arbutin)보다 큰 활성을 보여주었다. 이상의 결과들로부터 대황 추출물은 활성산소종을 소거하는 항산화제로 이용가능하며, 특히 아글리콘 분획의 현저한 항산화작용 및 큰 타이로시네이즈 저해 효과로부터 이들 분획 또한 화장품원료로서 응용 가능성이 큼을 알 수 있었다.