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Antioxidative Effect and Tyrosinase Inhibitory Activity of Rheum undulatum L. Extracts

대황의 항산화 효과 및 타이로시네이즈 저해 활성

  • Kim, Jung-Eun (Department of Fine Chemistry, Nanobiocosmetic Lab., Cosmetic R&D Center, College of Nature and Life Science, Seoul National University of Science and Technology) ;
  • Park, Chan-Ha (Hansung Science High School) ;
  • Oh, Dae-Seok (Hansung Science High School) ;
  • Lee, Seung-Yeon (Hansung Science High School) ;
  • Jang, Se-Hun (Hansung Science High School) ;
  • Hong, Jee-Yeon (Hansung Science High School) ;
  • Min, Hye-Jin (Department of Fine Chemistry, Nanobiocosmetic Lab., Cosmetic R&D Center, College of Nature and Life Science, Seoul National University of Science and Technology) ;
  • Park, Su-Ah (Department of Fine Chemistry, Nanobiocosmetic Lab., Cosmetic R&D Center, College of Nature and Life Science, Seoul National University of Science and Technology) ;
  • Won, Doo-Hyun (Department of Fine Chemistry, Nanobiocosmetic Lab., Cosmetic R&D Center, College of Nature and Life Science, Seoul National University of Science and Technology) ;
  • Park, Soo-Nam (Department of Fine Chemistry, Nanobiocosmetic Lab., Cosmetic R&D Center, College of Nature and Life Science, Seoul National University of Science and Technology)
  • 김정은 (서울과학기술대학교 자연생명과학대학 정밀화학과 나노바이오화장품연구실 화장품종합기술연구소) ;
  • 박찬하 (한성과학고등학교) ;
  • 오대석 (한성과학고등학교) ;
  • 이승연 (한성과학고등학교) ;
  • 장세훈 (한성과학고등학교) ;
  • 홍지연 (한성과학고등학교) ;
  • 민혜진 (서울과학기술대학교 자연생명과학대학 정밀화학과 나노바이오화장품연구실 화장품종합기술연구소) ;
  • 박수아 (서울과학기술대학교 자연생명과학대학 정밀화학과 나노바이오화장품연구실 화장품종합기술연구소) ;
  • 원두현 (서울과학기술대학교 자연생명과학대학 정밀화학과 나노바이오화장품연구실 화장품종합기술연구소) ;
  • 박수남 (서울과학기술대학교 자연생명과학대학 정밀화학과 나노바이오화장품연구실 화장품종합기술연구소)
  • Received : 2011.11.02
  • Accepted : 2011.11.14
  • Published : 2011.12.30

Abstract

In this study, the antioxidative effects and inhibitory activities on tyrosinase of Rheum undulatum (R.undulatum) L. extracts were investigated. 50 % ethanol extract, ethyl acetate and aglycone fractions of R. undulatum L. were used in experiments. The DPPH (1,1-diphenyl-2-picrylhydrazyl) scavenging activities ($FSC_{50}$) of R. undulatum L. extracts was lower than (+)-${\alpha}$-tocopherol, known as a typical antioxidant. Reactive oxygen species (ROS) scavenging activities ($OSC_{50}$) of aglycone fraction on ROS generated in $Fe^{3+}$-EDTA/$H_2O_2$ system using the luminol-dependent chemiluminescence assay showed the most prominent effect at a concentration of $0.265\;{\mu}g/mL$. The cellular protective effects of extract/fractions of R. undulatum L. on the rose-bengal sensitized photohemolysis of human erythrocytes were increased in a concentration dependent manner ($1{\sim}50\;{\mu}g/mL$). Especially, aglycone fraction in $10\;{\mu}g/mL$ concentration showed the most protective effect among extracts (${\tau}_{50}$, 757.0 min). The inhibitory effects ($IC_{50}$, $11.20\;{\mu}g/mL$) on tyrosinase of aglycone fraction was much higher than arbutin ($226.88\;{\mu}g/mL$), known as a whitening agent. These results indicate that R. undulatum L. extracts can be used as antioxidant. Particularly, aglycone fraction of R. undulatum L. showed superior antioxdative activity and high inhibitory effect on tyrosinase. Therefore, aglycone fraction of R. undulatum L. could be applicable to new functional cosmetics.

본 연구에서는 대황 추출물의 항산화 활성, 타이로시네이즈(tyrosinase) 저해 활성을 확인하였다. 대황의 50 %에탄올 추출물, 에틸아세테이트(ethyl acetate) 분획, 아글리콘(aglycone) 분획으로 실험을 진행하였다. 대황 추출물들의 DPPH (1,1-diphenyl-2-picrylhydrazyl) 소거활성($FSC_{50}$)은 대표적인 항산화제인 (+)-${\alpha}$-tocopherol보다 낮은 것으로 나타났다. Luminol 발광법을 이용한 $Fe^{3+}$-EDTA/$H_2O_2$ 계에서 생성된 활성산소종에 대한 아글리콘 분획의 소거활성(총 항산화능, $OSC_{50}$)은 $0.265\;{\mu}g/mL$로 매우 큰 활성을 나타내었다. 대황 추출물의 rose-bengal로 증감된 $^1O_2$에 의한 적혈구 파괴에 대한 세포보호 효과는 모든 분획에서 농도 의존적($1{\sim}50\;{\mu}g/mL$)으로 증가하였으며, 특히 아글리콘 분획은 $10\;{\mu}g/mL$ 농도에서 ${\tau}_{50}$이 757.0 min으로 높은 세포 보호 활성을 나타내었다. 대황 추출물 중 아글리콘 분획의 타이로시네이즈 저해활성($IC_{50}$)은 $11.20\;{\mu}g/mL$으로 $226.88\;{\mu}g/mL$인 알부틴(arbutin)보다 큰 활성을 보여주었다. 이상의 결과들로부터 대황 추출물은 활성산소종을 소거하는 항산화제로 이용가능하며, 특히 아글리콘 분획의 현저한 항산화작용 및 큰 타이로시네이즈 저해 효과로부터 이들 분획 또한 화장품원료로서 응용 가능성이 큼을 알 수 있었다.

Keywords

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