• 대한한의학회지
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• 제28권4호
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• pp.74-85
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• 2007

Objective : In this study we wanted to confirm if proper stimulation and de-Qi of traditional Korean medical acupuncture could increase hot flash relief efficacy. Design : A randomized controlled, single blind study. We used two modalities of acupuncture, one with optimal stimulation [Study group; Korean medical acupuncture (TKMA)] and one with minimal stimulation [Control group; Minimal acupuncture (MA)]. Same acupoints [PC6(內關), HT8(少府), HT7(神門), LI4(合谷), ST36(足三里), SP6(三陰交), Ren4(關元)] were used in both groups. Fifty-two patients were treated twice a week for 8 weeks, and follow up was done after 4 weeks from the last treatment. Patients were checked hot flash VAS (visual analog scale), frequency and duration every time they visited. Results : Hot flash relief efficacy by 100mm hot flash VAS was obvious in both groups. Hot flash VAS scores of study group were smaller than the scores of control group at the early stage (3rd, $4^{th}$ and $8^{th}$ visit), but there wasn't a remarkable difference between study and control group at the end of the trial. Besides, diminution of hot flash VAS was faster and more even in the study group than control group by visualization using 'Box plot'. We compared frequency and duration of hot flash, 100mm sweating, palpitation, sleep disturbance VAS, and Kupperman Index, MENQOL, Patient's global assessment score. Both groups showed definite decrease from the baseline, but the difference was not statistically significant. There wasn't any adverse event. Hot flash relief efficacy was kept in most patients after 4 weeks' follow-up. Conclusion : Acupoint combination by Traditional Korean medical theory is effective on hot flashes and hot flash relief efficacy was faster and more even in optimal stimulation than minimal stimulation.

• Journal of Microbiology and Biotechnology
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• 제26권12호
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• pp.2214-2223
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• 2016

Toll-like receptors (TLRs) can recognize conserved molecular patterns and initiate a wide range of innate and adaptive immune responses against invading infectious agents. The aim of this study was to assess the transcript profile of mink TLRs (mTLRs) in mink peripheral blood mononuclear cells (PBMCs) and a range of tissues, and to explore the potential role of mTLRs in the antiviral immune response process. The results indicated that the mTLR partial nucleotide sequences had a high degree of nucleotide identity with ferret sequences (95-98%). Phylogenetic analysis showed that mammalian TLRs grouped into five TLR families, with a closer relationship of the mTLRs with those of ferret than the other mammalian sequences. Moreover, all the mTLRs were ubiquitously expressed in lymphoid organs (spleen and lymph nodes) and PBMCs. Interestingly, the mTLR expression patterns in lung, uterus, and heart showed quite a lot of similarity. Another remarkable observation was the wide expression of mTLR1-3 mRNAs in all tissues. Among the analyzed tissues, skeletal muscle was revealed to being the lowest repertoire of mTLR expression. Additionally, mink PBMCs exposed to the canine distemper virus revealed significant upregulation of mTLR2, mTLR4, mTLR7, and mTLR8 mRNAs, indicating that mTLRs have a role in innate immunity in the mink. Collectively, our results are the first to establish the basic expression patterns of mTLRs and the relationship between mTLRs and a virus, which will contribute to better understanding of the evolution and the functions of mTLRs in the innate immune system in minks.

• Journal of Microbiology and Biotechnology
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• 제28권7호
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• pp.1122-1132
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• 2018

In this study, we attempted to find new and efficient microbial enzymes for producing rare sugars. A ribose-5-phosphate isomerase B (OsRpiB) was cloned, overexpressed, and preliminarily purified successfully from a newly screened Ochrobactrum sp. CSL1, which could catalyze the isomerization reaction of rare sugars. A study of its substrate specificity showed that the cloned isomerase (OsRpiB) could effectively catalyze the conversion of $\text\tiny{L}$-rhamnose to $\text\tiny{L}$-rhamnulose, which was unconventional for RpiB. The optimal reaction conditions ($50^{\circ}C$, pH 8.0, and 1 mM $Ca^{2+}$) were obtained to maximize the potential of OsRpiB in preparing $\text\tiny{L}$-rhamnulose. The catalytic properties of OsRpiB, including $K_m$, $k_{cat}$, and catalytic efficiency ($k_{cat}/K_m$), were determined as 43.47 mM, $129.4sec^{-1}$, and 2.98 mM/sec. The highest conversion rate of $\text\tiny{L}$-rhamnose under the optimized conditions by OsRpiB could reach 26% after 4.5 h. To the best of our knowledge, this is the first successful attempt of the novel biotransformation of $\text\tiny{L}$-rhamnose to $\text\tiny{L}$-rhamnulose by OsRpiB biocatalysis.

• The Plant Pathology Journal
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• 제31권2호
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• pp.140-151
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• 2015

Bacillus subtilis B006 strain effectively suppresses the cucumber fusarium wilt caused by Fusarium oxysporum f. sp. cucumerinum (Foc). The population dynamics of Foc, strain B006 and its surfactin over-producing mutant B841 and surfactin-deficient mutant B1020, in the rhizosphere were determined under greenhouse conditions to elucidate the importance of the lipopeptides excreted by these strains in suppressing Foc. Results showed that B. subtilis strain B006 effectively suppressed the disease in natural soil by 42.9%, five weeks after transplanting, whereas B841 and B1020 suppressed the disease by only 22.6% and 7.1%, respectively. Quantitative PCR assays showed that effective colonization of strain B006 in the rhizosphere suppressed Foc propagation by more than 10 times both in nursery substrate and in field-infected soil. Reduction of Foc population at the cucumber stems in a range of $0.96log_{10}ng/g$ to $2.39log_{10}ng/g$ was attained at the third and the fifth weeks of B006 treatment in nursery substrate. In field-infected soil, all three treatments with B. subtilis suppressed Foc infection, indicated by the reduction of Foc population at a range of $2.91log_{10}ng/g$ to $3.36log_{10}ng/g$ at the stem base, one week after transplanting. This study reveals that the suppression of fusarium wilt disease is affected by the effective colonization of the surfactin-producing B. subtilis strain in the rhizosphere. These results improved our understanding of the biocontrol mechanism of the B. subtilis strain B006 in the natural soil and facilitate its application as biocontrol agent in the field.

• 한국유기농업학회지
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• 제27권4호
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• pp.513-528
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• 2019

본 논문은 고수 에탄올 추출물의 항산화 활성 및 항염증 효과를 평가하기 위하여 수행되었다. 총 폴리페놀 및 플라보노이드 함량은 1 mg/mL 농도에서 각각 76.03 ± 1.36 mg of Gallic acid equivalents/g, 182.23±4.32 mg of Rutin equivalents/g으로 나타났고 DPPH와 ABTS radical 소거능은 각각 500 ㎍/mL, 1000 ㎍/mL 농도에서 52.8%, 58.3%의 소거율을 확인하였으며 2000 ㎍/mL 농도에서 환원력을 나타났다. 세포독성 검사 결과, LPS와 함께 처리한 세포에서 고수 추출물 (0-500 ㎍/mL)에 의한 독성이 나타나지 않았고 염증 매개인자인 NO, 염증성 cytokine인 TNF-α 및 IL-1β의 생성량을 농도의존적으로 유의하게 감소시켰다. 또한 염증성 단백질인 iNOS 및 COX-2의 발현 수준을 유의하게 감소시켰고 이런 염증성 단백질 발현을 조절하는 전사인자 NF-κB/MAPK signaling pathway의 활성화 수준도 유의하게 억제시킨 것으로 나타났다. 이와 같이 고수 에탄올 추출물의 항산화 및 항염증 활성을 나타냄으로써 활용 가치가 기능성 식품 및 대체 의약 소재로 이용될 수 있다고 사료된다.

• Journal of Microbiology
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• 제45권1호
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• pp.21-28
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• 2007

In order to search for specific genotypes related to this unique phenotype, we used whole genomic DNA microarray to characterize the genomic diversity of Helicobacter pylori (H. pylori) strains isolated from clinical patients in China. The open reading frame (ORF) fragments on our microarray were generated by PCR using gene-specific primers. Genomic DNA of H. pylori 26695 and J99 were used as templates. Thirty-four H. pylori isolates were obtained from patients in Shanghai. Results were judged based on In(x) transformed and normalized Cy3/Cy5 ratios. Our microarray included 1882 DNA fragments corresponding to 1636 ORFs of both sequenced H. pylori strains. Cluster analysis, revealed two diverse regions in the H. pylori genome that were not present in other isolates. Among the 1636 genes, 1091 (66.7%) were common to all H. pylori strains, representing the functional core of the genome. Most of the genes found in the H. pylori functional core were responsible for metabolism, cellular processes, transcription and biosynthesis of amino acids, functions that are essential to H. pylori's growth and colonization in its host. In contrast, 522 (31.9%) genes were strain-specific genes that were missing from at least one strain of H. pylori. Strain-specific genes primarily included restriction modification system components, transposase genes, hypothetical proteins and outer membrane proteins. These strain-specific genes may aid the bacteria under specific circumstances during their long-term infection in genetically diverse hosts. Our results suggest 34 H. pylori clinical strains have extensive genomic diversity. Core genes and strain-specific genes both play essential roles in H. pylori propagation and pathogenesis. Our microarray experiment may help select relatively significant genes for further research on the pathogenicity of H. pylori and development of a vaccine for H. pylori.

• Reproductive and Developmental Biology
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• 제31권3호
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• pp.145-152
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• 2007

본 연구에서는 SV40 Tag을 마우스 albumin 유전자의 promoter/enhancer 조절 하에 발현하도록 설계된 재조합 유전자를 마우스 1세포기 수정란에 미세 주입하여 형질 전환마우스를 제작하고 이들의 인체 간암 모델로써의 적합성을 조사하였다. 형질 전환이 확인된 총 11개체의 founder 생쥐들 중 4개체가 간암을 일으켰고, 두 개체는 신장암을, 한 개체는 피부 및 뇌에서 종양을 각각 일으켰다. 이들로부터 외래 유전자를 계대 유전하는 3가계를 얻었다(#1-2, #1-6, #1-11). 이들 가계의 자소들에서 8주령(#1-2, #1-6)혹은 10주령(#1-11)시부터 간암이 반복적으로 발생되었으며, #1-11 founder개체에서 폐로 암세포가 전이된 것 외에는 다른 조직에서의 형태학적 변이가 발견되지 않았다. 간암 발생은 조직학적 변화에 따라 3단계로 나눌 수 있었다. 즉, 출생에서 3주령까지는 간세포의 과량증식을 보이나 세포핵의 이상은 관찰되지 않았으며, 4주령부터 7주령(#1-2, #1-6) 혹은 9주령(#1-11)까지는 diffuse liver cell dysplasia를 나타내지만 tumor nodule은 발견되지 않았고, 그 이후에는 liver dysplasia를 배경으로 간암이 발생하였다. 본 연구에서 작출한 간암 모델 마우스는 인체 간암과 일부 유사한 소견을 보였는바 인체 간암 기전 연구를 위한 유용한 동물 모델로 이용할 수 있을 것으로 생각된다.

• 대한지역사회영양학회지
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• 제18권2호
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• pp.165-176
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• 2013

The objective of this study was to analyze the influence of socio-demographic factors on food consumption frequencies among adolescents in Korea. Data were obtained from the Seventh Korea Youth Risk Behavior Web-based Survey (2011 KYRBS), a nationwide representative sample of 75,643 (37,873 males and 37,770 females) middle and high school students. It was carried out as a self-administered on-line survey. The frequency of eating breakfast was 4.8 times per week for middle school students and 4.6 times per week for high school students (p < 0.001). Higher levels of perceived household economic status, family affluence scale (FAS) and education attainment of mother were associated with more frequent breakfast eating. The frequencies of consumption of vegetables and milk were higher in males than in females (p < 0.001). The frequency of consumption fruits was higher in females than in males (p < 0.01). Higher levels of perceived household economic status, FAS and education attainment of mother were associated with more frequent consumption of vegetable, fruits and milk. The frequencies of consumption of soda, fast food and instant noodls were higher in males than in females (p < 0.001). The frequency of consumption of snacks was higher in females than in males. Adolescents with lower levels of FAS and education attainment of mother were at risk for skipping breakfast and consuming of soda, fast food and instant noodls more frequently. Whereas, adolescents with higher levels of FAS, education attainment of mother were more likely to be frequent consumers of vegetable, fruits and milk. These findings demonstrated that being high school students and belonging to lower level of socio-economic status (SES) were associated with undesirable food habits.

• 센서학회지
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• 제7권2호
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• pp.111-116
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• 1998

리튬 이온 전도체를 이용하여 히터가 내장된 단순한 형태의 고체전해질 이산화탄소 감지소자를 제작하였다. 소자의 기준전극과 감지전극은 Au를 사용하였다. type ( I )과 type (II)의 두가지 형태의 소자로 구분하여 제조하였다. type ( I ) 소자는 알카리 금속 탄산염을 $420{\sim}500^{\circ}C$에서 융착하여 제조하였다. Type (II) 소자의 감지막은 알카리 금속 탄산염을 결착제와 혼합한 후 감지전극에 도포하여 제조하였다. 제조된 이산화탄소 감지소자를 동작온도 $420^{\circ}C$에서 이산화탄소 농도 950 ppm에서 9,950 ppm의 영역에 걸쳐 응답특성을 조사하였다. type ( I ) 소자와 type (II) 소자는 이산화탄소 농도변화에 대하여 각각 62 mV/decade와 65 mV/decade의 감도를 보였다. Type (II) 소자의 기전력값의 변화는 동작온도 $420^{\circ}C$에서 Nernst 식의 이론적인 값에 거의 일치하였고, $15{\sim}20$초 이내의 빠른 응답시간을 가지면서 매우 안정한 응답특성을 보였다. 또한 type (II) 소자는 60일 동안 우수한 장기 안정도와 재현성을 보였다.

• 한국수산과학회지
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• 제35권5호
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• pp.490-496
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• 2002

한국, 미국, 일본지역에 서식하는 연어에서 추출한 genomic DNA를 이용하여 연어의 mtDNA NDI 영역, D-loop 영역, growth hormone, IGF-I, MCH2, histone H3의 염기서열을 분석하여, 최적의 primer를 제작하여 PCR을 실시한 결과, mtDNA NDI 영역은 Ks12, Ks24, As11, As14, Js13, Js15에서 증폭된 DNA를 확인하였으며, D-loop 영역, growth hormone, IGF-I, histone H3, MCH2에서는 모든 시료에서 증폭된 DNA를 확인하였다. DGGE 분석의 결과, mtDNA NDI 영역 (AF133701, 449-880), D-loop 영역 (AF125518, 11-514)과 growth hormone (AFO05927, 181-530)에서는 이형다양성을 확인하였으며, IGF-I (AF063216, 962-1461)과 MCH2 (M27281, 70-593)는 모두 이형다양성이 나타났으나, histone H3 (AF017147, 7-487)는 모두 이형다양성이 관찰되지 않았다. 그리고 real time PCR 관찰 결과는 DGGE의 결과와 유사한 점을 찾을 수 없었지만, real time PCR도 각각의 유전자에 따라 서로 다른 DNA 생성 패턴을 보여 DNA 변이를 쉽게 구별하는데 보조적인 도움이 되었다.