• Journal of Microbiology and Biotechnology
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• v.26 no.6
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• pp.989-998
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• 2016

Acidic endo-polygalacturonases are the major part of pectinase preparations and extensively applied in the clarification of fruits juice, vegetables extracts, and wines. However, most of the reported fungal endo-polygalacturonases are active and stable under narrow pH range and low temperatures. In this study, an acidic endo-polygalacturonase (EPG4) was purified and characterized from a mutant strain of Penicillium oxalicum. The N-terminal amino acid sequence of EPG4 (ATTCTFSGSNGAASASKSQT) was different from those of reported endo-polygalacturonases. EPG4 displayed optimal pH and temperature at 5.0 and 60-70℃ towards polygalacturonic acid (PGA), respectively, and was notably stable at pH 2.2-7.0. When tested against pectins, EPG4 showed enzyme activity over a broad acidic pH range (>15.0% activity at pH 2.2-6.0 towards citrus pectin; and >26.6% activity at pH 2.2-7.0 towards apple pectin). The K_m and V_max values were determined as 1.27 mg/ml and 5,504.6 U/mg, respectively. The enzyme hydrolyzed PGA in endo-manner, releasing oligo-galacturonates from PGA, as determined by TLC. Addition of EPG4 (3.6 U/ml) significantly reduced the viscosity (by 42.4%) and increased the light transmittance (by 29.5%) of the papaya pulp, and increased the recovery (by 24.4%) of the papaya extraction. All of these properties make the enzyme a potential application in the beverage industry.

• The Korean Journal of Physiology and Pharmacology
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• v.20 no.5
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• pp.507-514
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• 2016

Although 3D-complex fractionated atrial electrogram (CFAE) mapping is useful in radiofrequency catheter ablation for persistent atrial fibrillation (AF), the directions and configuration of the bipolar electrodes may affect the electrogram. This study aimed to compare the spatial reproducibility of CFAE by changing the catheter orientations and electrode distance in an in -silico left atrium (LA). We conducted this study by importing the heart CT image of a patient with AF into a 3D-homogeneous human LA model. Electrogram morphology, CFAE-cycle lengths (CLs) were compared for 16 different orientations of a virtual bipolar conventional catheter (conv-cath: size 3.5 mm, inter-electrode distance 4.75 mm). Additionally, the spatial correlations of CFAE-CLs and the percentage of consistent sites with CFAE-CL<120 ms were analyzed. The results from the conv-cath were compared with that obtained using a mini catheter (mini-cath: size 1 mm, inter-electrode distance 2.5 mm). Depending on the catheter orientation, the electrogram morphology and CFAE-CLs varied (conv-cath: $11.5{\pm}0.7%$ variation, mini-cath: $7.1{\pm}1.2%$ variation), however the mini-cath produced less variation of CFAE-CL than conv-cath (p<0.001). There were moderate spatial correlations among CFAE-CL measured at 16 orientations (conv-cath: $r=0.3055{\pm}0.2194$ vs. mini-cath: $0.6074{\pm}0.0733$, p<0.001). Additionally, the ratio of consistent CFAE sites was higher for mini catheter than conventional one ($38.3{\pm}4.6%$ vs. $22.3{\pm}1.4%$, p<0.05). Electrograms and CFAE distribution are affected by catheter orientation and electrode configuration in the in-silico LA model. However, there was moderate spatial consistency of CFAE areas, and narrowly spaced bipolar catheters were less influenced by catheter direction than conventional catheters.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.12 no.7
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• pp.3030-3038
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• 2011

This study was conducted as descriptive research to examine the relations among beauty business workers' professional identity, emotional labor and depression, to analyze factors that influence their depression, and to provide basic information necessary to develop interventions for beauty business workers' depression. The subjects of this study were 158 beauty business workers sampled from 25 beauty shops in Seoul. Data were collected using a structured questionnaire during the period from April 12 to May 13, 2011. From the collected data were obtained real numbers, percentages, and the means and standard deviations of professional identity, emotional labor and depression using the SPSS 16.0 Program. In addition, multiple regression analysis was performed to determine the correlations among the variables and to identify factors influencing the beauty business workers' depression. In the results of multiple regression analysis on factors that influence the subjects' depression, factors found to explain their depression were autonomy among the sub factors of professional identity, and the diversity of emotional expressions and surface acting among the sub factors of emotional labor, and these factors explained 23.6% of their depression. The subjects' professional identity was in a positive correlation with the frequency of emotional expressions and deep acting among the sub factors of emotional labor, and in a negative correlation with surface acting and depression. The Frequency of emotional expression and the diversity of emotional expressions which the sub factors of emotional labor were in a negative correlation with depression and in a positive correlation with surface acting and depression. In order to relieve beauty business workers from their depression based on these results, there should be education or programs for establishing beauty business workers' professional identity and program for increasing of emotional expressions and decreasing of surface acting.

• Journal of Korea Multimedia Society
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• v.14 no.4
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• pp.494-503
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• 2011

In this paper, we propose a recognition application of facial expression for laughter theraphy on smartphone. It detects face region by using AdaBoost face detection algorithm from the front camera image of a smartphone. After detecting the face image, it detects the lip region from the detected face image. From the next frame, it doesn't detect the face image but tracks the lip region which were detected in the previous frame by using the three step block matching algorithm. The size of the detected lip image varies according to the distance between camera and user. So, it scales the detected lip image with a fixed size. After that, it minimizes the effect of illumination variation by applying the bilateral symmetry and histogram matching illumination normalization. After that, it computes lip eigen vector by using PCA(Principal Component Analysis) and recognizes laughter expression by using a multilayer perceptron artificial network. The experiment results show that the proposed method could deal with 16.7 frame/s and the proposed illumination normalization method could reduce the variations of illumination better than the existing methods for better recognition performance.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.12 no.7
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• pp.1227-1234
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• 2008

In this paper is designed a 256-bit synchronous OTP(one-time programmable) memory required in application fields such as automobile appliance power ICs, display ICs, and CMOS image sensors. A 256-bit synchronous memory cell consists of NMOS capacitor as antifuse and access transistor without a high-voltage blocking transistor. A gate bias voltage circuit for the additional blocking transistor is removed since logic supply voltage VDD(=1.5V) and external program voltage VPPE(=5.5V) are used instead of conventional three supply voltages. And loading current of cell to be programmed increases according to RON(on resistance) of the antifuse and process variation in case of the voltage driving without current constraint in programming. Therefore, there is a problem that program voltage can be increased relatively due to resistive voltage drop on supply voltage VPP. And so loading current can be made to flow constantly by using the current driving method instead of the voltage driving counterpart in programming. Therefore, program voltage VPP can be lowered from 5.9V to 5.5V when measurement is done on the manufactured wafer. And the sens amplifier circuit is simplified by using the sens amplifier of clocked inverter type instead of the conventional current sent amplifier. The synchronous OTP of 256 bits is designed with Magnachip $0.13{\mu}m$ CMOS process. The layout area if $298.4{\times}314{\mu}m2$.

• Food Science and Preservation
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• v.24 no.3
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• pp.374-380
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• 2017

The quality characteristics of 'Xiangshui' pears (Pyrus ussuriensis) treated with different concentrations of biomass pyrolysis liquid (BPL) during storage at $25^{\circ}C$ were investigated. The weight of 'Xiangshui' pears treated with BPL declined at a slower rate than that of the control. The rot index of BPL-treated 'Xiangshui' pears decreased with increasing storage times, and treatment with 20-fold-diluted BPL resulted in the lowest rot index after storage for 12 days. The total acid content of 'Xiangshui' pears treated with 20-fold-diluted BPL was 0.19%, and was the highest after storage for 12 days. After storage for 12 days, the total sugar content of 'Xiangshui' pears treated with 20-fold-diluted BPL was 7.19%; this was significantly higher than that of the control, but not significantly different from that of pears treated with other BPL dilutions. The vitamin C content of 'Xiangshui' pears showed a decreased trend, and pears treated with 20-fold-diluted BPL had a vitamin C content of 2.21 mg/100 g after storage for 12 days and showed the least decline compared to other treatments. In addition, respiration in 'Xiangshui' pears was effectively inhibited by treatment with BPL. In conclusion, BPL treatment exerts a protective effect on the quality of 'Xiangshui' pears during storage, with 20-fold-diluted BPL being the most effective.

• The Journal of the Korea Contents Association
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• v.21 no.9
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• pp.419-430
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• 2021

The purpose of this study was to examine the effects of Adlerian Encouragement Program on the interpersonal satisfaction, self-efficacy and self-esteem among elementary school students. The program was consisted of 8 sessions: 1^st session was for introducing programs and making positive nicknames, 2^nd~5^th sessions were for abandoning inferiority and discouraged thoughts, displaying posters of success experiences, creating merit trees, 6^th~7^th sessions were for self-encouragement and other people encouragement, 8^th session was for closing the program. Subjects were 40 students who were the fifth grader students at I elementary school in G city. The subjects were divided into an experimental group(N=21) and a control group(N=19). All participants were asked to complete the Interpersonal Satisfaction scale(Kim, 2010), Self-efficacy scale(Han, 2003) and Self-esteem scale(Choi & Jeon, 1993). The pretest and post-test were taken in order to verify the effectiveness of the program. The data were analyzed with ANCOVA. The results are as follows: the Adlerian Encouragement Program had a significant impact on interpersonal satisfaction and self-esteem but didn't show a significant impact on self-efficacy. The study confirmed that the Adlerian Encouragement Program had a significant effect on interpersonal satisfaction and self-esteem of elementary school students, suggesting the encouragement, a key technique in Adlerian psychology, is an important educational philosophy and method for healthy development and democratic class management.

• Journal of Digital Convergence
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• v.17 no.7
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• pp.423-431
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• 2019

The objective of this study was to survey Chinese university students taking a required taekwondo course, with a goal to understand the impact of motives for participating in required university physical education courses on the subjective sense of well-being of the students. To achieve this end, the current study selected 256 students participating in a required taekwondo course in a university located in Shaanxi Province, China, as final effective samples. SPSS 22.0 was used to process the data, and the analysis results are as follows: First, Chinese university students' motives of participating in required physical education courses were positively correlated with their subjective well-being. More specifically, cognitive motivation, intellectual motivation, and social motivation, in that order, had significant correlation with positive emotions. Second, intellectual motivation was found to have a positive correlation with sense of satisfaction in life, while cognitive motivation had a negative correlation with sense of satisfaction in life. Third, intellectual and cognitive motivations were found to influence positive emotions, but social motivation was found not to have an influence on all areas of subjective sense of well-being. In addition, intellectual, cognitive, and social motivations were found not to have an influence on negative emotions.

• Journal of Microbiology and Biotechnology
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• v.29 no.7
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• pp.1043-1052
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• 2019

Active lipase-producing bacterium Burkholderia gladioli Bps-1 was rapidly isolated using a modified trypan blue and tetracycline, ampicillin plate. The electro-phoretically pure enzyme was obtained by purification using ethanol precipitation, ion-exchange chromatography, and gel filtration chromatography. The molecular weight was 34.6 kDa and the specific activity was determined to be 443.9 U/mg. The purified lipase showed the highest activity after hydrolysis with $p-NPC_{16}$ at a pH of 8.5 and $50^{\circ}C$, and the $K_m$, $k_{cat}$, and $k_{cat}/K_m$ values were 1.05 mM, $292.95s^{-1}$ and $279s^{-1}mM^{-1}$, respectively. The lipase was highly stable at $7.5{\leq}pH{\leq}10.0$. $K^+$ and $Na^+$ exerted activation effects on the lipase which had favorable tolerance to short-chain alcohols with its residual enzyme activity being 110% after being maintained in 30% ethanol for 1 h. The results demonstrated that the lipase produced by the strain B. gladioli Bps-1 has high enzyme activity and is an alkaline lipase. The lipase has promising chemical properties for a range of applications in the food-processing and detergent industries, and has particularly high potential for use in the manufacture of biodiesel.

• Korean Journal of Environment and Ecology
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• v.32 no.6
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• pp.676-685
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• 2018

This study is to compare carbon budget between serpentine sites and non-serpentine sites dominated by Pinus densiflora forest in the Andong serpentine area where has high values of magnesium and low values of calcium, and are usually deficient in nitrogen and phosphorus, but rich in heavy metals such as nickel, chrome, cobalt, etc. and to measure soil $CO_2$ efflux and environmental factors between January 2017 and December 2017. Soil $CO_2$ efflux was measured with LI-6400 once a month; the soil temperature at 10 cm depth, air temperature, soil moisture contents, and solar radiation were measured in continuum. Soil $CO_2$ efflux in the serpentine area and non-serpentine were $151.71{\pm}75.09g\;CO_2{\cdot}m^{-2}month^{-1}$(42.48 ~ 262.61 g $CO_2{\cdot}m^{-2}month^{-1}$) and $165.09{\pm}118.96g\;CO_2{\cdot}m^{-2}month^{-1}$(20.94 ~ 449.24 g $CO_2{\cdot}m^{-2}month^{-1}$), respectively. Carbon storage in the serpentine area and non-serpentine area were 91.90, $222.85ton{\cdot}ha^{-1}$, respectively. Carbon absorption in the serpentine area and non-serpentine area were 7.99, $17.41ton{\cdot}ha^{-1}yr^{-1}$, respectively. Carbon budget in the serpentine area and non-serpentine area were absorbs 5.3, $14.49ton{\cdot}Cha^{-1}yr^{-1}$, respectively.