• 대한금속재료학회지
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• 제46권6호
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• pp.338-344
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• 2008

The crystallization kinetics of the $Cu_{43}Zr_{43}Al_7Be_7$ bulk metallic glass were studied by differential scanning calorimetry(DSC) in the continuous heating and isothermal annealing modes. Only one major peak could be detected on the DSC traces of $Cu_{43}Zr_{43}Al_7Be_7$ bulk amorphous alloy, and the activation energy for crystallization corresponding to the peak determined by the Kissinger method was resulted of 239 kJ/mol. The isothermal kinetic, analyzed by the Johnson-Mehl-Avrami equation yielded values for the Avrami exponents in the range 1.69 to 2.37, which implied a crystallization governed by a three-dimensioned growth. Primary phases were essentially the cubic structure CuZr together with the $Cu_{10}Zr_7$ phase. At higher temperature, the CuZr disappeared while the $Cu_{10}Zr_7$ became predominant. After long term annealing at 731 K, the phases were $Cu_{10}Zr_7$, $Cu_2ZrAl$ and $Al_3Zr_5$.

• Asian Pacific Journal of Cancer Prevention
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• 제16권7호
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• pp.2671-2674
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• 2015

Thyroid cancer is the most prevalent endocrine cancer and is evident in nearly 5% of thyroid nodules. The correlation between mean platelet volume (MPV) and many other cancer types has been investigated previously. However, the correlation between papillary thyroid carcinoma (PTC) and MPV has not yet been studied in detail. The aim of this study was to examine whether MPV would be a useful inflammatory marker to differentiate PTC patients from cases of benign goiter and healthy controls. Preoperative MPV levels in patients with PTC were found to be significantly higher when compared with benign goiter patients and healthy controls ((respectively, 8.05 femtoliter (fl), 7.57 fl, 7.36 fl, p=0.001). After surgical treatment of PTC patients, a significant decrease in MPV levels was seen (8.05 fl versus 7.60 fl, p=0.005). ROC analysis suggested 7.81 as the cut-off value for MPV (AUC=0.729, sensitivity 60%, specificity 80%). In conclusion, maybe changes in MPV levels can be used as an easily available biomarker for monitoring the risk of PTC in patients with thyroid nodules, enabling early diagnosis of PTC.

• 교육시설
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• 제16권4호
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• pp.71-78
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• 2009

After the introduction of the 7th revision of curriculum, educational facilities have been requested to change to face new curriculums and have been planned. But, it is actual condition that researches of curriculum and facilities seem to be insufficient. This research will re-establish in an angle of space-syntax based on existed research for home-base area of variation type in high school according to the 7th curriculum. And the each specific character of home-base area representing each type and strong / weak points of area structure of each home-base will be analyzed. And analysis result with the model of variation type in high school visit the on-the-spot inspection result which leads and comparison and with his interview back it leads and currently there is the objective to the evaluation the variation type in high school home-base which is planned regarding the suitable in real use. Each case to the school I visited the scene, not all high schools applied the 7th curriculum courses. Therefore, it is also home-base space capabilities were not being properly utilize. According to 7th curriculum, one of Hall-type home-base and Room-type home-base can be suitable when integration is higher. However, the way the current operational training, it is the best that Distributed-type home-base needs to be included in each curriculum block.

• Journal of Microbiology and Biotechnology
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• 제24권2호
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• pp.188-196
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• 2014

Extracellular production of recombinant human bone morphogenetic protein-7 (rhBMP-7) was carried out through the fermentation of Bacillus subtilis. Three significant fermentation conditions and medium components were selected and optimized to enhance the rhBMP-7 production by using the response surface methodology (RSM). The optimum values of the three variables for the maximum extracellular production of rhBMP-7 were found to be 2.93 g/l starch, 5.18 g/l lactose, and a fermentation time of 34.57 h. The statistical optimization model was validated with a few fermentations of B. subtilis in shake flasks under optimized and unoptimized conditions. A 3-L jar fermenter using the shake-flask optimized conditions resulted in a higher production (413 pg/ml of culture medium) of rhBMP-7 than in a shake flask (289.1 pg/ml), which could be attributed to the pH being controlled at 6.0 and constant agitation of 400 rpm with aeration of 1 vvm.

• 통합자연과학논문집
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• 제11권2호
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• pp.87-92
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• 2018

5-Hydroxytryptamine receptor 7 ($5-HT_7R$) is one of G-Protein coupled receptors, which is found to be involved in the pathophysiology of various neurological disorders including depression, sleep disorders, memory deficiency and neuropathic pain. After activation of $5-HT_7R$ by serotonin, it activates the production of the intracellular signaling molecule cyclic AMP. The availability of 3D structure of the receptor would enhance the development of new drugs. Hence, in the present study, homology modelling of human 5-hydroxytryptamine receptor 7 ($5-HT_7R$) was performed using comparative modelling (Easy Modeller) and threading (I-TASSER) approaches. The generated models were validated using Ramachandran plot and ERRAT plot and the best models were selected based on the validation results. The 3D model developed here could be useful for identifying crucial residues and further docking study.

• Bulletin of the Korean Chemical Society
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• 제20권7호
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• pp.809-814
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• 1999

Computations are reported for the very floppy cumulenones H2CnO (n = 3-7). The structures, harmonic frequencies and dipole moments are computed using the DFT and MP2 methods. H2C6O and H2C7O are treated for the first time. The carbon skeletons in ground states of these molecules with smaller number of carbon atoms are found to be bent, but very floppy. Those of the cumulenones with higher number of carbon atoms are predicted to be nearly linear. All the molecules are predicted to be of Cs symmetry by the present results. Good agreement with the available experimental observations is obtained.

• 미생물과산업
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• 제14권1호
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• pp.7-11
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• 1988

Efficient in vitro RNA synthesis can be easily accomplished from cloned DNA using bactrio-phage SP6, T7 or T3 RNA polymerase. Despite its popularity as in vitro transcription system, molecular mechanisms of bacteriophage transcription has not been studied, although physical and catalytic properties of several phage RNA polymerases have well been documented (1). Only recently the T7 promoter has been physically mapped by footprinting of the T7 RNA polymerase (2,3). These simple phage systems, however, could be useful for detailed molecular studies of transcription.

• Journal of the korean society of oceanography
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• 제38권4호
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• pp.157-165
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• 2003

In order to understand the characteristics of sedimentary environments in Ulleung Basin of the East Sea (Sea of Japan), three sediment cores were taken with a box corer during R/V Tamyang cruise in October 1999. Activities of $^{7}Be,{\;}^{210}Pb,{\;}^{226}Ra,{\;}^{234}Th,{\;}^{238}{\;}and{\;}^{137}Cs$ in sediment samples were determined by non-destructive gamma-ray spectrometry. Rates of sedimentation and particle mixing were estimated by best fitting an advection­diffusion particle mixing model to the data of $^{7}Be,{\;}^{234}Th,{\;}and{\;}^{210}Pb$. Estimated sedimentation rates were 0.06-0.08 cm/yr and particle mixing rates were $0.13-0.65{\;}\textrm{cm}^2/yr$. The use of multiple tracers in our study prevented us from probable up to 38% overestimation of sedimentation rates.

• Journal of Microbiology and Biotechnology
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• 제8권4호
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• pp.369-377
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• 1998

Rotavirus is a major cause of severe gastroenteritis in young children and animals throughout the world. The VP7 of rotavirus is thought to induce the synthesis of neutralizing antibodies and to be responsible for determining viral serotypes. The cDNA coding for the VP7 capsid protein of human rotavirus, obtained from Korean patients (HRV-Y14), was cloned and its nucleotide sequence was determined. Comparative analysis of the nucleotide sequences between VP7 of Y14 and that of other foreign isolates showed $92.7~95.2\%$ homology to G1 serotypes (RV-4, KU, K8, WA), $74.2\%$ homolgy to G2 serotype HU-5, $76.4\%$ homology to G3 serotype SA-11, and $77.6\%$ homology to G4 serotype A01321. These data suggest that HRV-Y14 can be classified as a G1 serotype. cDNA coding for VP7 of HRV-YI4 was subcloned into the baculovirus vector and the VP7 glycoprotein was expressed in insect cells. The expressed proteins in Sf9 cell extract and tissue culture fluid were separated on SDS-PAGE, and Western blot analysis with monoclonal antibody raised against the synthetic peptide containing 21 amino acids within the VP7 conserved region was performed. The molecular weight of recombinant VP7 was estimated to be 36 kDa which is about the same size as the native VP7. Addition of tunicamycin in the culture media caused a reduction of the molecular weight of the recombinant VP7 indicating that the expressed protein was glycosylated.

• 대한화학회지
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• 제47권1호
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• pp.67-71
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• 2003

7차 교육과정에 따라 개발된 7학년 과학교과서 물질 영역의 탐구활동을 분석하여 교과서의 개발과 학습에 대한 교육적 시사점을 찾고자 하였다. 교과서의 탐구활동을 탐구유형, 탐구과정, 탐구상황 등의 3개의 영역으로 나누어 분석하였다. 6개 출판사에서 발간된 과학교과서의 ‘물질의 세 가지 상태', ‘분자운동', ‘상태변화와 에너지'를 분석 대상으로 하였다. 7학년 물질 영역의 탐구 활동 분석 결과를 종합하였을 때, 탐구 유형이나 탐구 과정 요소 및 탐구 상황의 편중성이 두드러졌으며, 이러한 부분을 보완할 수 있는 탐구 활동으로 학생들을 지도하여야 할 것이다. 또한, 과학과의 교육 목표에 부합되는 교과서 개발을 위해 탐구 활동을 검토하고 교과서 검정 기준에 탐구 활동의 평가틀을 제공하여야 할 것이다.