• 대한기계학회논문집B
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• 제28권12호
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• pp.1549-1556
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• 2004

We have deigned, fabricated and compared four different types of static chaos microfluid mixers, including the mixers using straight channel flow, microblock-induced alternating whirl flow, microchannel-induced lamination flow, and combined alternating whirl-lamination flow. Among them, the alternating whirl-lamination (AWL-type) mixer, composed of 3-D rotationally arranged microblocks and dividing microchannels fabricated by conventional planar lithography process, is effective to reduce the mixing length over wide flow rate ranges. We characterize the performance of the fabricated mixers, through the flow visualization technique using phenolphthalein solution. We verify that the AWL-type microfluid mixer shows the shortest fluid mixing length of 2.8mm∼5.8mm for the flow rate range of Re=0.26∼26 with the pressure drop lower than 5kPa. Compared to the previous mixers, requiring the mixing lengths of 7∼17mm, the AWL-type microfluid mixer results in the 60% reduction of the mixing lengths. Due to the reduced mixing lengths within reasonable pressure drop ranges, the present micromixers have potentials for use in the miniaturized Micro-Total-Analysis-Systems($\mu$TAS).

• 대한기계학회논문집B
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• 제30권2호
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• pp.110-116
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• 2006

A syringe pump or a device using high electric voltage has been used for controlling flows inside a LOC (lab-on-a-chip). Compared to LOC, however, these microfluidic devices are large and heavy that they are burdensome for a portable ${\mu}-TAS$ (micro total analysis system). In this study, a new flow control technique employing pressure regulators and pressure chambers was developed. This technique utilizes compressed air to control the micro-scale flow inside a LOC, instead of a mechanical actuator or an electric power supply. The pressure regulator controls the output air pressure by adjusting the variable resistor attached. We checked the feasibility of this system by measuring the flow rate inside a capillary tube of $100{\mu}m$ diameter in the Re numbers ranged from 0.5 to 50. In addition, the performance of this flow control system was compared with that of a conventional syringe pump. The developed flow control system was found to show superior performance, compared with the syringe pump. It maintains automatically the: air pressure inside a pressure chamber whether the flow inside the capillary tube is on or off. Since the flow rate is nearly proportional to the resistance, we can control flow in multiple microchannels precisely. However, the syringe pump shows large variation of flow rate when the fluid flow is blocked in the microchannel.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2005년도 춘계학술대회 논문집
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• pp.1824-1827
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• 2005

For application to micro fluid control systems such as ${\mu}TAS$ (Micro Total Analysis Systems) and DDS (Drug Delivery Systems), it is very significant to handle precise and minute flow rates with low pressure pulsation. In this study, a novel valveless piezoelectric pump using peristaltic motion with three disk type PZT actuators is presented. The newly devised pump with an effective size of $70mm{\times}60mm{\times}55mm$ has three actuator layers connected in series from inlet to outlet. The PZT actuator has a maximum displacement of 240 ${\mu}m$ and a maximum force of 1.6 N. When the driving voltage for PZT actuators is sequentially applied with a certain phase shift, the pumping is performed by peristaltic motion of liquid volume. The working fluid is shut off without the driving voltage. Three methods for sequential driving are proposed and experimentally investigated. First and second methods utilize an intermittent sinusoidal waveform with phase shift of $90{\circ}\;and\;120^{\circ}$, respectively. Third method uses a rectangular waveform with phase shift of $90^{\circ}$. A controller with multi-phase shifter is designed and fabricated. Then, frequency and voltage-flow rate characteristics and load pressure-flow rate characteristics are experimentally investigated to verify the validity of the developed pump.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2006년도 제37회 하계학술대회 논문집 C
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• pp.1629-1630
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• 2006

This paper describes a micro total analysis system ($\mu$ TAS) for detecting and digesting the target protein which includes a bead based temperature controllable microchip and computer based controllers for temperature and valve actuation. We firstly combined the temperature control function with a bead based microchip and realized the on-chip sequential reactions using two kinds of beads. The PEG-grafted bead, on which RNA aptamer was immobilized, was used for capturing and releasing the target protein. The target protein can be chosen by the type of RNA aptamer. In this paper, we used the RNA aptamer of HCV replicase. The trypsin coated bead was used for digesting the released protein prior to the matrix assisted laser desorption ionization time of flight mass spectrometer (MALDI TOF MS). Heat is applied for release of the captured protein binding on the bead, thermal denaturation and trypsin digestion. PDMS microchannel and PDMS micro pneumatic valves were also combined for the small volume liquid handling. The entire procedures for the detection and the digestion of the target protein were successfully carried out on a microchip without any other chemical treatment or off-chip handling using $20\;{\mu}l$ protein mixture within 20 min. We could acquire six matched peaks (7% sequence coverage) of HCV replicase.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2006년도 제37회 하계학술대회 논문집 C
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• pp.1645-1646
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• 2006

Micro ElectroMechanical Systems (MEMS) 기술을 이용한 초미세 유체 제어 시스템 (마이크로 펌프, 마이크로 밸브, 마이크로 채널, 마이크로 믹서 등)은 화학, 생명분야의 DNA 분석, 항원-항체 분석, 질병의 진단 등에 사용되는 lab-on-a-chip, micro total analysis system ($\mu$-TAS) 등에서 화학 및 바이오 유체를 제어하는 분석 시스템의 일부분으로서 사용되며 필수적으로 요구된다. 본 논문에서는 이러한 microchip을 구현하기 위해 초미세 유체 제어 소자인 마이크로 펌프와 밸브를 같은 기관 위에 polydimethylsiloxane (PDMS)와 indium tin oxide (ITO)-Glass를 사용하여 동일한 구조로 집적 하였다. 마이크로 펌프의 pumping rate은 인가 직류 펄스 전력의 주파수와 duty 비를 변화시켜 최적화하였다. 직류 펄스 전력 500 mW를 인가하였을 때 주파수 2 Hz, duty 비 7 %에서 약 $1.05{\mu}l/min$의 최대 유량이 측정되었다. 마이크로 밸브는 ITO 히터에 전력을 인가함으로서 유량의 on/off 제어가 잘 됨을 확인할 수 있었고 유체를 closing하기 위해 필요한 전력은 약 300 mW이다.

• 대한기계학회논문집B
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• 제34권1호
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• pp.77-82
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• 2010

소형분석시스템에서 미세채널의 상판이 제거되면 상판에 의한 빛 에너지 손실이 대폭 감소되어 광학측정법으로 대상을 분석할 때 장점을 갖는다. 본 연구에서는 상판이 없는 사각단면 미세채널 내 액체유동을 이해하려는 목적으로 실험과 전산유체역학 해석을 수행하였다. 개방형 사각단면 미세채널에서 입자추적기법으로 유속을 측정하였고, 채널의 단면적 변화에 따른 모세관 유동현상을 이론적으로 해석하였다. 단면의 너비와 높이가 각각 20 ${\mu}m$로 제작된 미세채널의 주입부에 물을 떨어뜨렸을 때 물은 오직 모세관 힘에 의해 미세채널을 따라 이동하였다. 액체의 젖음현상에 영향을 미치는 중요한 유동 파라미터는 채널의 크기와 표면장력, 점성 등으로 볼 수 있으며, 미세채널에서 액체 유동을 조절하는데 이용될 수 있다.

• Journal of Community Nutrition
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• 제6권2호
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• pp.103-109
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• 2004

Body antioxidant status is an important factor for the prevention of many chronic diseases in the elderly. This study was done to investigate antioxidant status and its relationship to immune response by measuring plasma cytokine (IL-2 and IL-6) levels in elderly women. Subjects were 76 elderly women aged over 60 years, visiting Jangwhi Social Welfare Center of Seongbook-Gu in Seoul. Subjects were divided into 3 groups according to age (< 65, 65 - 74, > 75). Dietary intakes were assessed by semi-quantitative food frequency questionnaires (SFFQ). Plasma vitamin C level was measured by 2,4-dinitrophenylhydrazine method and plasma levels of vitamin E, A and ${\beta}$-carotene were measured by HPLC. Plasma levels of IL-2 and IL-6 were determined with a solid phase sandwich enzyme linked-immuno-sorbent assay (ELISA) using commercial kits. The average intakes of antioxidant vitamins were 96.3mg (137.5% of RDA) for vitamin C and 523.3 ${\mu}$gRE (74.8% of RDA) for vitamin A in elderly women. All of the average plasma levels of antioxidant vitamins were within normal range. However the percentage of the elderly women with deficiency plus marginal values were 7.9% in vitamin C, 9.2% in vitamin A and 7.9% in vitamin E. Plasma levels of IL-2 and IL-6 were 27.1${\pm}$7.1pg/ml and 5.9${\pm}$5.3pg/ml in elderly women. Correlation data showed that plasma IL-2 level was negatively correlated with plasma vitamin C level. In addition, IL-6 level was also negatively correlated with plasma vitamin C, A and E levels, respectively. There was a significant positive correlation between erythrocyte thiobarbituric acid-reactive substance(TB-ARS) level and plasma IL-2 or IL-6 levels. In addition, erythrocyte TBARS level showed a significant positive correlation with plasma total antioxidant status (TAS) level and a significant negative correlation with plasma vitamin C level. Overall results might imply that the decreased levels of antioxidant vitamins result in an increase in oxidative stress and thereby increase cytokine production such as IL-2 and IL-6. However further research is required to elucidate these relationships.