• Biotechnology and Bioprocess Engineering:BBE
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• v.11 no.3
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• pp.258-261
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• 2006

The addition of zeolite particles enhances the stability of mRNA molecules in a cell-free protein synthesis system. When $20{\mu}g/{\mu}L$ of zeolite (Y5.4) is added to a reaction mixture of cell-free protein synthesis, a substantial increase in protein synthesis is observed. The stabilizing effect of zeolite is most dearly observed in an in vitro translation reaction directed by purified mRNA, as opposed to a coupled transcription and translation reaction. Upon the addition of zeolite in the in vitro translation reaction, the life span of the mRNA molecules is substantially extended, leading to an 80% increase in protein synthesis. The effect of zeolite upon the mRNA stability appears be strongly related to the cation exchange (potassium to sodium) reaction. Our results demonstrate the possibility of modifying this biological process using heterogeneous, non-biological substances in a cell-free protein synthesis system.

• Journal of Periodontal and Implant Science
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• v.34 no.2
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• pp.345-356
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• 2004

The triclosan was shown to have anti-microbial and anti-inflammatory effect with inhibition of inflammatory mediators such as prostaglandin $E_2(PGE_2)$. The purpose of this study was to elucidate whether and how $PGE_2$ could be inhibited by triclosan in human gingival fibroblast. Human gingival fibroblast-1 cells (ATCC CRL2014) were pre-treated for 1 hour with triclosan (0.001 ${\mu}/ml{\sim}10$ ${\mu}/ml$) and then stimulated with $TNF-{\alpha}$ (1.0 ng/ml). $PGE_2$ synthesis was evaluated by ELISA and gene expression of COX-1 and COX-2 was evaluated by RT-PCR after $TNF-{\alpha}$, triclosan, and NS-398 (COX-2 inhibitor, 5, ${\mu}M$) and/ or cycloheximide (protein synthesis inhibitor, 2 ${\mu}g/ml$). Triclosan was cytotoxic to human gingival fibroblasts in the concentration higher than 1.0 ${\mu}g/ml$ for longer than 24 hours in tissue culture. The $PGE_2$ synthesis was inhibited by triclosan in dose-dependent manner. Greater COX-2 mRNA suppression was observed with triclosan (0.1 ${\mu}g/ml$) than with $TNF-{\alpha}$ alone, without change in COX-1 gene expression. Inhibitory effects of triclosan on $PGE_2$ synthesis disappeared in presence of cycloheximide. This study suggests that triclosan inhibit prostaglandin $E_2$ at the level of COX-2 gene regulation and require de novo protein synthesis.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2001.04a
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• pp.275-278
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• 2001

Research on the forced vibration control of a flexible GFR composite beam using $\mu$-synthesis is performed on this paper. Modal analysis method and modal coordinates are introduced to obtain the state equations of the structural system. Using these equations, Robust control algorithm using $\mu$-synthesis is adopted to suppress the forced vibration of a flexible beam since the designed controller can considered plant uncertainty and external disturbance. Constant disturbance which is generated by shaking the flexible beam as I's natural frequency is effectively rejected by a PZT actuator. Simulations and experiments are carried out with the designed controller and effectiveness of forced vibration suppression strategy is verified by results.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2000.11a
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• pp.11-14
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• 2000

This paper presents the vibration control of a glass-fiber reinforced thermoplastic composite beam with a distributed PVDF sensor and piezo-ceramic achlator. The three types of different controllen which are PID, H$\infty$ , and p-synthesis ontrollcr are employed to achieve vibration suppression in the transient vibration of composite beam. In the H$\infty$ , controller design, 1st and 2nd natural frequencies are considered in the modeling, because robust control theory which has robustness to struchred uncertainty is adopled Lo suppress the vibration. If the controller designed by H$\infty$ , theory does not satisfy control performance, it is improved by $\mu$ -synthesis method with D-K iteration so that the$\mu$-contoller based on the structured singular value satisfies the nominal performance and robust performance Simulations and experiments were carried out with the designed controllers m order to demonstrate the suppression efficiency of each controller.

• International Journal of Precision Engineering and Manufacturing
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• v.1 no.2
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• pp.76-83
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• 2000

A study on the robust control of a composite beam with a distributed PVDF sensor and piezo-ceramic actuator is presented in this paper. $1^{st}$ and $2^{nd}$ natural frequencies are considered in the modeling, because robust control theory which has robustness to structured uncertainty is adopted to suppress the vibration. If the controllers designed by $H_{\infty}$ theory do not satisfy control performance, it is improved by $\mu$-synthesis method with D-K iteration so that the $\mu$-controller based on the structured singular value satisfies the nominal performance and robust performance. Simulation and experiment were carried out with the designed controller and the verification of the robust control properties was presented by results.

• The Korean Journal of Zoology
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• v.20 no.2
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• pp.93-99
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• 1977

Dose response forDNA repair synthesis induced by various concentrations of MMC (0.05 $\\sim$ 0.5 $\\mu$g/ml) in HeLa $S_3$ cells was not dose-dependent and the amounts of it were relatively lower, representing $7\\sim9%$ of total DNA synthesizing cells in $0.1\\sim0.5 \\mug/ml$ concentrations. Time dependence study showed that MMC-induced DNA repair synthesis occurred as long as for 24 hours with similar incidences in all time courses. Pretreatment with BUdR was found to have a sensitization effect on MMC-induced DNA repair synthesis, but that with IUdR was not. Combined treatment with BUdR of IUdR and MMC suppressed remarkably the semiconservative DNA synthesis especially at later time course. These results seem to suggest that damages induced in DNA by MMC might be repaired by both fast and slow excision processes.

• Journal of the Korea Convergence Society
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• v.8 no.5
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• pp.1-11
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• 2017

With a given spring-mass-damper system, $H_{\infty}$ and ${\mu}$-synthesis control methods are used to build system controllers which minimize vibrations at two major natural frequencies in two cases; without uncertainty; with 20% uncertainty. In order to check the stability and performance of two controllers, those are examined using GM and PM values. The signal strength of output responses is compared using the concept of central numerical differentiation and then results are quantified using the RMS method. Lastly, 40 random samples of $H_{\infty}$ and ${\mu}$-synthesis controllers are obtained for three different $W_{per\;f1}$ weighting functions and drawn in the time domain in order to compare the stability. Overall, ${\mu}$-synthesis controller manages the vibrations much better than $H_{\infty}$ controller according to the robust stability and performance values obtained by simulating random samples of 40 plant models.

• YAKHAK HOEJI
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• v.34 no.5
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• pp.365-373
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• 1990

The effects of the protein fraction of Panax ginseng on primary cultured chicken embryonic brain cells and DRG cultured with a deficient medium were studied. The protein fraction was further fractionated into four groups according to the molecular weight; larger than 10,000 dalton(fraction A), between 5,000 and 10,000 daltons(fraction B), between 1,000 and 5,000 daltons(fraction C), between 500 and 1,000 daltons(fraction D). All four protein fractions at the concentration of $100\;{\mu}g/ml$ significantly increased the number of the brain cells which promoted the neurite outgrowth. The activity of PDHC in the brain cells was elevated significantly by the protein fraction B at the concentration of $100\;{\mu}g/ml$. It was noted that $100\;{\mu}g/ml$ protein fraction C and D significantly enhanced the synthesis of protein in the brain cells. At the concentration of $100\;{\mu}g/ml$, the protein fraction B enhanced RNA synthesis and the protein fraction A significantly enhanced DNA synthesis in the brain cells. The protein fractions B, C, and D significantly promoted the neurite outgrowth of DRG at the concentration of $100\;{\mu}g/ml$.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.44 no.1
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• pp.57-69
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• 2016

There are three kinds of algorithms(2-ARE, mu-synthesis, LMI) for controller design using closed-loop shaping method. This paper provides the summary of background theory of three algorithms and $H_{\infty}$ controller design results for spacecraft attitude control using the three controller design tools of Matlab$^{TM}$ Toolbox for comparison. As a result, it reveals that LMI design method is more reliable as well as easier than others for spacecraft attitude control design. Comparison results are as follow: 2-ARE method and LMI method provide almost same results in robust stability, robust performance and control authority level. But 2-ARE method is more sensitive than LMI method with respect to proper design of weighting functions: 2-ARE method is more difficult than LMI method in weighting function design. The design result of mu-synthesis method shows worse performance and requires bigger control authority than others.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2004.11a
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• pp.574-577
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• 2004

This paper presents a hybrid system combining lead rubber bearings and hydraulic actuators controlled by a $\mu-synthesis$ method for seismic response control of a cable-stayed bridge. A hybrid system could alleviate some of restrictions and limitations that exist when each system is acting alone because multiple control devices are operating. Therefore, the overall control performance of a hybrid system may be improved compared to each system, however the overall system robustness may be negatively impacted by active device in the hybrid system or active controller may cause instability due to small margins. Therefore, a $\mu-synthesis$ method that guarantees the robust performance is considered to enhance the possibility of real applications of the control system. The performances of the proposed control system are compared with those of passive, active, semiactive control systems and hybrid system controlled by a LQG algorithm. Furthermore, an extensive robust analysis with respect to stiffness and mass matrices perturbation and time delay of actuator is performed. Numerical simulation results show that the performances of the proposed control system are superior to those of passive system and slightly better than those of active and semiactive systems and two hybrid systems show similar control performances. Furthermore, the hybrid system controlled by a f-synthesis method shows the good robustness without loss of control performances. Therefore, the proposed control system could effectively be used to seismically excited cable-stayed bridge which contains many uncertainties.