• Korean Journal of Pharmacognosy
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• v.34 no.2 s.133
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• pp.156-160
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• 2003

$NF-{\kappa}B$ (nuclear factor-kappa B) plays a particularly central role in epidermal biology. It has been established that ultraviolet radiation (UVR) is one of the mechanisms to induce the activation of $NF-{\kappa}B$ in human skin. We previously demonstrated that melanogenic inhibitors may act through the inhibition of $NF-{\kappa}B$ activation in keratinocytes. In order to find another type of melanogenic inhibitors of $NF-{\kappa}B$ activation, various kinds of the extracts from crude drugs $(30\;{\mu}g/ml)$ were preincubated with transfectant HaCaT cells for 3 hrs and then UVR $(60\;mj/cm^2)$ was irradiated. UVR-exposed cells were incubated for another 6 hrs to measure the $NF-{\kappa}B$ activity. $NF-{\kappa}B$ activation was measured with the secreatory alkaline phosphates (SEAP) reporter gene assay using a fluorescence detection method. Among natural products, Lycium chinense, Acanthopanax senticosus, Angelica koreana, Kalopanax pictus and Asparagus cochinchinensis were the most potent inhibitors of $NF-{\kappa}B$ activation by UVR. These observations suggest that some crude drugs might act partially through the modulation of the synthesis of melanotrophic factors to decrease melanogenesis in keratinocytes.

• The Korean Journal of Physiology and Pharmacology
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• v.7 no.2
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• pp.65-71
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• 2003

Increasing evidences suggest that ischemia-induced vascular damage is an integral step in the cascade of the cellular and molecular events initiated by cerebral ischemia. In the present study, employing a mouse brain endothelioma-derived cell line, bEnd.3, and oxygen-glucose deprivation (OGD) as an in vitro stroke model, the role of nuclear factor kappa B (NF-${\kappa}B$) activation during ischemic injury was investigated. OGD was found to activate NF-${\kappa}B$ and to induce bEnd.3 cell death in a time-dependent manner. OGD phosphorylated neither 32 Ser nor 42 Tyr of $I{\kappa}B{\alpha}$. OGD did not change the amount of $I{\kappa}B{\alpha}$. The extents of OGD-induced cell death after 8 h, 10 h, 12 h and 14 h of OGD were 10%, 35%, 60% and 85%, respectively. Reperfusion following OGD did not cause additional cell death, indicating no reperfusion injury after ischemic insult in cerebral endothelial cells. Three known as NF-${\kappa}B$ inhibitors, including pyrrolidine dithiocarbamate (PDTC) plus zinc, aspirin and caffeic acid phenethyl ester (CAPE), inhibited OGD-induced NF-${\kappa}B$ activation and increased OGD-induced bEnd.3 cell death in a dose dependent manner. There were no changes in the protein levels of bcl-2, bax and p53 which are modulated by NF-${\kappa}B$ activity. These results suggest that NF-${\kappa}B$ activation might be a protective mechanism for OGD-induced cell death in bEnd.3.

• Food Science and Biotechnology
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• v.15 no.2
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• pp.303-307
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• 2006

Effect of Oenanthe javanica ethanol extract (OJE) on nuclear factor-${\kappa}B$ (NF-${\kappa}B$)-mediated inflammatory reaction in RAW 264.7 macrophage cells was investigated. The OJE dose-dependently inhibited secretions of tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) and prostaglandins $E_2\;(PGE_2)$ from lipopolysaccharide (LPS)-stimulated RAW 264.7 cells and blocked LPS-induced expression of cyclooxygenase-2. To clarify mechanistic basis for its inhibitions of NF-${\kappa}B$ and activator protein-1 (AP-1) activations, effects of OJE on activations of NF-${\kappa}B$ and AP-1 genes by luciferase reporter activity were examined. The LPS-stimulated activations of NF-${\kappa}B$ and AP-1 were significantly blocked by 400 and $600\;{\mu$}g/mL of OJE, implicating that OJE might regulate gene expression through more than one signaling pathway. Cytosolic degradation of I-${\kappa}B{\alpha}$ was inhibited by OJE dose-dependently, indicating that the nuclear translocation of p65 was inhibited by OJE. These findings suggest that the inhibition of LPS-stimulated COX-2 expression by OJE is due to its inhibition of NF-${\kappa}B$ activation by blocking I-${\kappa}B{\alpha}$ degradation, which may be mechanistic basis of anti-inflammatory effects of OJE.

• Molecules and Cells
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• v.20 no.2
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• pp.241-246
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• 2005

PDK-1 activates PI3-kinase/Akt signaling and regulates fundamental cellular functions, such as growth and survival. NF-${\kappa}B$ is involved in the induction of a variety of cellular genes affecting immunity, inflammation and the resistance to apoptosis induced by some anti-cancer drugs. Even though the crucial involvement of the PI3-kinase/Akt pathway in the anti-apoptotic activation of NF-${\kappa}B$ is well known, the exact role of PDK-1 as well as PI3-kinase/Akt in NF-vactivation is not understood. Here we demonstrate that PDK-1 plays a pivotal role in transcriptional activation of NF-${\kappa}B$ by dissociating the transcriptional co-repressor HDAC1 from the p65 subunit of NF-${\kappa}B$. The association of CBP with p65 was not directly modulated by PDK-1 or by PI3-kinase. Etoposide activated NF-${\kappa}B$ through PI3-kinase/Akt, and the transcription activation domain (TAD) of p65 was further activated by wild-type PDK-1. Overexpression of a dominant negative PDK-1 mutant decreased etoposide-induced NF-${\kappa}B$ transcription and further down-regulated the ectopic HDAC1-mediated decrease in NF-${\kappa}B$ transcriptional activity. Thus activation of PDK-1 relieves the HDAC1-mediated repression of NF-${\kappa}B$ that may be related to basal as well as activated transcription by NF-${\kappa}B$. This effect may also explain the role of the PI3-kinase/PDK-1 pathway in the anti-apoptotic function of NF-${\kappa}B$ associated with the chemoresistance of cancer cells.

• Journal of Physiology & Pathology in Korean Medicine
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• v.21 no.4
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• pp.1017-1024
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• 2007

Thymus and activation-regulated chemokine (TARC/CCL-17), produced by keratinocytes, is a CC chemokine known to selectively Th2 type T cells via $CCR4^+$ and is implicated in the development of atopic dermatitis (AD). TARC/CCL17 expression was induced by cytokines such as tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) and interferon-${\gamma}$ (IFN-${\gamma}$). We recently found that the wogonin, a flavone isolated from Scutellaria baicalensis, suppressed TARC expression via heme oxygenase 1 (HO1) in human keratinocytes induced with mite antigen. However, little is known about the inhibitory mechanism of wogonin on TARC/CCL-17 expression stimulated with cytokines. To investigate the inhibitory mechanism, I determined the inhibitory effects of wogonin on the activation of nuclear factor-${\kappa}B$ (NF-${\kappa}B$) and $I{\kappa}B{\alpha}$ phosphorylation, and also examined the activation of p38 MAP kainase in HaCaT keratinocytes stimulated with TNF-${\alpha}$ and IFN-${\gamma}$. Wogonin inhibited NF-${\kappa}B$-DNA complex, NF-${\kappa}B$ binding activity, and the phosphorylation of $I{\kappa}B{\alpha}$ in a dose dependent manner. Wogonin also inhibited the translocation of NF-${\kappa}B$ from cytosol to nucleus. Moreover, the phosphorylation of of p38 MAP kinase in the TNF-${\alpha}$ and IFN-${\gamma}$-stimulated HaCaT keratinocytes were suppressed by wogonin in a dose dependent manner. These results suggest that wogonin may inhibit cytokine-induced NF-${\kappa}B$ activation by $I{\kappa}B{\alpha}$ degradation via suppression of p38 MAP kinase signaling pathway in keratinocytes and modulation of wogonin signaling pathway may be beneficial for the treatment of AD.

• Journal of the Korean Mathematical Society
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• v.38 no.3
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• pp.623-631
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• 2001

Let $\kappa$ be a real abelian field of conductor f and $\kappa$(sub)$\infty$ = ∪(sub)n$\geq$0$\kappa$(sub)n be its Z(sub)p-extension for an odd prime p such that płf$\phi$(f). he aim of this paper is ot compute the cohomology groups of circular units. For m>n$\geq$0, let G(sub)m,n be the Galois group Gal($\kappa$(sub)m/$\kappa$(sub)n) and C(sub)m be the group of circular units of $\kappa$(sub)m. Let l be the number of prime ideals of $\kappa$ above p. Then, for mm>n$\geq$0, we have (1) C(sub)m(sup)G(sub)m,n = C(sub)n, (2) H(sup)i(G(sub)m,n, C(sub)m) = (Z/p(sup)m-n Z)(sup)l-1 if i is even, (3) H(sup)i(G(sub)m,n, C(sub)m) = (Z/P(sup)m-n Z)(sup l) if i is odd (※Equations, See Full-text).

• Proceedings of the KSME Conference
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• 2003.04a
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• pp.1980-1985
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• 2003

Two nonlinear ${\kappa}-{\epsilon}$ models with the wall function method are applied to the fully developed turbulent flow in a square duct. Typical predicted quantities such as axial and secondary velocities, turbulent kinetic energy and Reynolds stresses are compared in details both qualitatively and quantitatively with each other. A nonlinear ${\kappa}-{\epsilon}$ model with the wall function method capable of predicting accurately duct flows involving turbulence-driven secondary motion is presented in the present paper. The nonlinear ${\kappa}-{\epsilon}$ model adopted in a commercial code is found to be unable to predict accurately duct flows with the prediction level of secondary flows one order less than that of the experiment.

• Communications of the Korean Mathematical Society
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• v.26 no.2
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• pp.197-205
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• 2011

Explicit sufficient conditions are established for the oscillation of the one order neutral differential equations with impulsive $(x(t)+{\sum\limits^n_{i=1}}c_ix(t-{\sigma}_i))'+px(t-{\tau})=0$, $t{\neq}t_{\kappa}$, ${\Delta}(x(t_{\kappa})+{\sum\limits^n_{i=1}}c_ix(t_{\kappa}-{\sigma}_i))+p_0x(t_{\kappa}-{\tau})=0$, $c_i{\geq}0$, $i=1,2,{\ldots}n$, $p{\tau}$>0, $p_0{\tau}$>0, ${\Delta}(x_{\kappa})=x(t^+_{\kappa})-x(t_{\kappa})$. Explicit sufficient and necessary condition are established when $c_i$ = 0, i = 1, 2, ${\ldots}$, n.

• Nonlinear Functional Analysis and Applications
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• v.26 no.3
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• pp.553-563
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• 2021

As hypergeometric meromorphic multivalent functions of the form $$L^{t,{\rho}}_{{\varpi},{\sigma}}f(\zeta)=\frac{1}{{\zeta}^{\rho}}+{\sum\limits_{{\kappa}=0}^{\infty}}{\frac{(\varpi)_{{\kappa}+2}}{{(\sigma)_{{\kappa}+2}}}}\;{\cdot}\;{\frac{({\rho}-({\kappa}+2{\rho})t)}{{\rho}}}{\alpha}_{\kappa}+_{\rho}{\zeta}^{{\kappa}+{\rho}}$$ contains a new subclass in the punctured unit disk ${\sum_{{\varpi},{\sigma}}^{S,D}}(t,{\kappa},{\rho})$ for -1 ≤ D < S ≤ 1, this paper aims to determine sufficient conditions, distortion properties and radii of starlikeness and convexity for functions in the subclass $L^{t,{\rho}}_{{\varpi},{\sigma}}f(\zeta)$.

• Communications of the Korean Mathematical Society
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• v.26 no.4
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• pp.623-634
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• 2011

The object of the present paper is to study N(${\kappa}$)-quasi Einstein manifolds. Existence of N(${\kappa}$)-quasi Einstein manifolds are proved. Physical example of N(${\kappa}$)-quasi Einstein manifold is also given. Finally, Weyl-semisymmetric N(${\kappa}$)-quasi Einstein manifolds have been considered.