• Title/Summary/Keyword: $(P.S.)^*_c$ condition

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Gene cloning of β-mannanase C from Cellulosimicrobium sp. YB-43 and characterization of the enzyme (Cellulosimicrobium sp. YB-43으로부터 mannanase C 유전자의 클로닝과 효소 특성)

  • Yoon, Ki-Hong
    • Korean Journal of Microbiology
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    • v.54 no.2
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    • pp.126-135
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    • 2018
  • The characteristics of enzyme and gene for mannanase B had been reported from Cellulosimicrobium sp. YB-43 producing some kind of mannanase. A gene coding for the enzyme, named mannanase C (ManC), was expected to be located downstream of the manB gene. The manC gene was cloned by polymerase chain reaction and sequenced completely. From this nucleotide sequence, ManC was identified to consist of 448 amino residues and contain a carbohydrate binding domain CBM2 besides a catalytic domain, which was homologous to mannanase belonging to the glycosyl hydrolase family 5. The catalytic domain of ManC showed the highest amino acid sequence similarity of 55% with the mannanases from Streptomyces sp. SirexAA-E (55.8%; 4FK9_A) and S. thermoluteus (57.6%; BAM62868). The His-tagged ManC (HtManC) lacking N-terminal signal peptide with hexahistidine at C-terminus was produced and purified from cell extract of recombinant Escherichia coli. The purified HtManC showed maximal activity at $65^{\circ}C$ and pH 7.5, with no significant change in its activity at pH range from 7.5 to 10. HtManC showed more active on konjac and locust bean gum (LBG) than guar gum and ivory nut mannan (ivory nut). Vmax and Km values of the HtManC for LBG were 68 U/mg and 0.45 mg/ml on the optimal condition, respectively. Mannobiose and mannotriose were observed on TLC as major products resulting from the HtManC hydrolysis of mannooligosacharides. In addition, mannobiose and mannose were commonly detected as the hydrolyzed products of LBG, konjac and ivory nut.

The Gettering Effect of Boron Doped n-type Monocrystalline Silicon Wafer by In-situ Wet and Dry Oxidation

  • Jo, Yeong-Jun;Yun, Ji-Su;Jang, Hyo-Sik
    • Proceedings of the Korean Vacuum Society Conference
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    • 2012.08a
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    • pp.429-429
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    • 2012
  • To investigate the gettering effect of B-doped n-type monocrystalline silicon wafer, we made the p-n junction by diffusing boron into n-type monocrystalline Si substrate and then oxidized the boron doped n-type monocrystalline silicon wafer by in-situ wet and dry oxidation. After oxidation, the minority carrier lifetime was measured by using microwave photoconductance and the sheet resistance by 4-point probe, respectively. The junction depth was analyzed by Secondary Ion Mass Spectrometry (SIMS). Boron diffusion reduced the metal impurities in the bulk of silicon wafer and increased the minority carrier lifetime. In the case of wet oxidation, the sheet resistance value of ${\sim}46{\Omega}/{\Box}$ was obtained at $900^{\circ}C$, depostion time 50 min, and drive-in time 10 min. Uniformity was ~7% at $925^{\circ}C$, deposition time 30 min, and drive-in time 10 min. Finally, the minority carrier lifetime was shown to be increased from $3.3{\mu}s$ for bare wafer to $21.6{\mu}s$ for $900^{\circ}C$, deposition 40 min, and drive-in 10 min condition. In the case of dry oxidation, for the condition of 50 min deposition, 10 min drive-in, and O2 flow of 2000 SCCM, the minority carrier lifetime of 16.3us, the sheet resistance of ${\sim}48{\Omega}/{\Box}$, and uniformity of 2% were measured.

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Design of Face Recognition algorithm Using PCA&LDA combined for Data Pre-Processing and Polynomial-based RBF Neural Networks (PCA와 LDA를 결합한 데이터 전 처리와 다항식 기반 RBFNNs을 이용한 얼굴 인식 알고리즘 설계)

  • Oh, Sung-Kwun;Yoo, Sung-Hoon
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.61 no.5
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    • pp.744-752
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    • 2012
  • In this study, the Polynomial-based Radial Basis Function Neural Networks is proposed as an one of the recognition part of overall face recognition system that consists of two parts such as the preprocessing part and recognition part. The design methodology and procedure of the proposed pRBFNNs are presented to obtain the solution to high-dimensional pattern recognition problems. In data preprocessing part, Principal Component Analysis(PCA) which is generally used in face recognition, which is useful to express some classes using reduction, since it is effective to maintain the rate of recognition and to reduce the amount of data at the same time. However, because of there of the whole face image, it can not guarantee the detection rate about the change of viewpoint and whole image. Thus, to compensate for the defects, Linear Discriminant Analysis(LDA) is used to enhance the separation of different classes. In this paper, we combine the PCA&LDA algorithm and design the optimized pRBFNNs for recognition module. The proposed pRBFNNs architecture consists of three functional modules such as the condition part, the conclusion part, and the inference part as fuzzy rules formed in 'If-then' format. In the condition part of fuzzy rules, input space is partitioned with Fuzzy C-Means clustering. In the conclusion part of rules, the connection weight of pRBFNNs is represented as two kinds of polynomials such as constant, and linear. The coefficients of connection weight identified with back-propagation using gradient descent method. The output of the pRBFNNs model is obtained by fuzzy inference method in the inference part of fuzzy rules. The essential design parameters (including learning rate, momentum coefficient and fuzzification coefficient) of the networks are optimized by means of Differential Evolution. The proposed pRBFNNs are applied to face image(ex Yale, AT&T) datasets and then demonstrated from the viewpoint of the output performance and recognition rate.

Thermal Stability of Superconductor NdBCO Sintered at Various Oxygen Partial Pressures (다양한 산소분압에서 소결한 NdBCO 초전도체의 열적 안정성)

  • Chung, J.K.;Kim, W.J.;Park, S.C.;Kang, S.G.;Lim, Y.J.;Kim, C.J.
    • Progress in Superconductivity
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    • v.10 no.2
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    • pp.133-138
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    • 2009
  • The $Nd_{1+x}Ba_{2-x}Cu_3O_{7-{\delta}}$(Nd123) superconductor exhibits high performance in high magnetic field and high temperature. We have studied phase stability for Nd123 under reduced oxygen partial pressure and various heat-treatment conditions. The main phase is Nd123 and some samples contain small amounts of Nd422 depending on the temperature and oxygen partial pressure. The decomposition temperature decreases with decreasing oxygen partial pressure from $1052^{\circ}C(P(O_2)$=150 Torr) to about $845^{\circ}C(P(O_2)$=0.1 Torr). The liquidus line was steeper temperature with decreasing oxygen partial pressure. In same condition of oxygen partial pressure, the region of stable Nd123 phase was formed at slightly higher temperature than the region of stable YBCO phase.

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Iontophoretic Delivery of Vitamine C 2-Phosphate (이온토포레시스를 이용한 2-인산 비타민 C의 피부투과)

  • Kim, Su-Youn;Oh, Seaung-Youl
    • Journal of Pharmaceutical Investigation
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    • v.34 no.3
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    • pp.201-207
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    • 2004
  • In order to develop an optimum formulation for iontophoretic flux of vitamine C 2-phosphate (VCP), we have prepared three different hydrogels containing VCP, using carbopol, HPMC and poloxamer, and iontophoretic flux through hairless mouse skin from these hydrogels was carried out. Drug stability in phosphate buffer (PBS) solution (pH 7.4) with and without current application was studied. The effect of various factors, such as drug concentration, current density, and current profile on skin flux was also investigated. Stability study indicated that VCP in PBS (pH 7.4) solution was stable under the experimental condition, irrespective of the presence of current. Cathodal delivery increased the flux markedly, whereas the anodal and passive flux was negligible. Thus, cathodal delivery was used in all experiments. Flux increased as the drug concentration (2.5, 5.0, 7.5%) and current density $(0.2,\;0.4,\;0.6\;mA/cm^2)$ increased. Pulsed application of the current showed lower flux than constant current application. The results obtained suggest that VCP can be delivered into the skin and the amount delivered can be controlled by varying hydrogel, current density, drug concentration and current application profile.

Design of Efficient Monitoring and Diagnosis System for Motor Condition Monitoring (모터의 상태 감시를 위한 효율적인 모터 감시 및 진단 시스템의 설계 및 구현)

  • Choi, J.H.;Park, H.S.;Lee, D.Z.;Eo, S.Y.;Kang, T.K.;Jeong, H.P.;Choi, J.P.
    • Proceedings of the KIEE Conference
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    • 2006.10a
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    • pp.167-168
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    • 2006
  • 본 논문은 현장에 운전 중인 모터를 효율적으로 고압 모터 감시 및 진단 기법을 검토하고 현장에 보다 적합한 고압모터 감시 및 진단 시스템을 설계 및 개발에 관하여 기술하였다. 고압 모터의 감시 및 진단 방법은 MCSA(Motor Current Signature Analysis) 기법을 기반으로 하여 순시전력, 공극토크, 진동분석 등 다양한 기법이 있으며 이 기법을 적용한 시스템이 출시되고 있다. 그러나 사용자의 요구 및 편이가 반영되지 않아 그대로 적용하기에는 어려움이 있다. 따라서 본 논문에서는 국내의 실정 및 사용자의 편이 맞도록 진단 시스템을 개발 필요성이 요구되어 다양한 검출 요소를 종합적으로 이용한 보다 현장에 적합하고 유용한 모터 감시 시스템을 설계하였다.

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Studies of Cyclosporin A Biosynthesis under the Conditions of Limited Dissolved Oxygen or Carbon Source in Fed-batch Culture (용존산소 제한 또는 탄소원 제한 조건의 유가식배양에서의 Cyclosporin A 생합성 연구)

  • 전계택;박성관;권호균;정연호;정용섭;장용근;이영행
    • KSBB Journal
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    • v.13 no.2
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    • pp.203-208
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    • 1998
  • We investigated the effects of dissolved oxygen (D.O.) and fructose (C-source) on cell growth and biosynthesis of cyclosporin A (CyA) produced as a secondary metabolite by a wild-type filamentous fungus, Tolypocladium inflatum. This was performed by controlling the level of D.O. and the residual C-source, as required, through adjustment of medium flow rate, medium concentration and agitation rate in fed-batch cultures. CyA production was furned out to be maximal, when D.O. level was controlled around 10% saturated D.O. and concentration of the C-source was maintained sufficiently low (below 2 g/L) not to cause carbon catabolite repression. Under this culture condition, we obtained the highest values of CyA concentration (507.14 mg/L), Qp (2.11 mg CyA/L/hr), $Y_x/s$ (0.49 g DCW/g fructose), $Y_p/s$<(22.56 mg CyA/g fructose), and YTEX>$_p/x$ (48.31 mg CyA/g DCW), but relatively lower values of cell concentration (11.98 g DCW/L) and cell productivity (0.043 g DCW/L/hr), in comparison with other parallel fed-batch fermentation conditions. These results implied that, in the carbon-limited culture with 10% saturated D.O. level, the producer microorganism utilized the C-source more efficiently for secondary metabolism.

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The Study on DISC with Direct and Indirect Social Support of Dental Hygienists (치과위생사의 행동유형(DISC)과 사회적 간접·직접지지와의 관계)

  • Kim, Ji-Hee;Kim, Hye-Jin;Yum, Jong-Hwa
    • The Korean Journal of Health Service Management
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    • v.8 no.2
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    • pp.127-137
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    • 2014
  • The purpose of this study is to investigate the relationship between behavior patterns(DISC) and direct indirect of social support from dental hygienists. The data used in this study were obtained from 171 dental hygienists who have been working on dental hospital or clinic in Pusan and Gyeongsangnam-do from 1st July to 30th August in 2011. In the level of direct indirect of social support according to subjects' general characteristics, higher educational level and non-religion was related with higher in direct of social support(p<0.05), and hygienists who worked in general hospital was related with higher indirect of social support(p<0.01). According to between behavior patterns(DISC) and indirect social support, these score were that I-type 3.55, D-type 3.53, C-type 3.41, and S-type 3.36. All behavior patterns(DISC) were similar with the indirect support. In direct support these score were that S-type 2.98 I-type 2.95, D-type and C-type 2.91. In frequency of behavior patterns(DISC), these were that I-type 56.7%, S-type 16.4%, C-type 14%, and D-type 12.9%. In correlation between factors, direction social support was correlated with I-type(p<.01) and C-type(p<.01) of behavior patterns(DISC). On based on these results, if the change of working environment, working condition will improve social support of dental hygienists. And education program have to developed for dental hygienists.

Evaluation of Undrained Shear Strength of Busan New-port Clay by DMT (DMT를 이용한 부산신항 점토의 비배수 전단강도 추정)

  • Hong, Sung-Jin;Shin, Dong-Hyun;Kim, Dong-Hee;Jung, Sang-Jin;Lee, Woo-Jin
    • Journal of the Korean Geotechnical Society
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    • v.23 no.7
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    • pp.87-98
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    • 2007
  • A series of dilatometer test, field vane test, and $CK_0U$ triaxial test were performed for clayey soils of Busan new port site to develop the relationships between undrained shear strength and the DMT results. Normalized undrained shear strength is turned out to be $S_{u(CKU)}/{\sigma}'_v=0.30{\sim}0.35\;for\;CK_0U$ triaxial test and ${\mu}S_{u(VST)}/{\sigma}'_v=0.20{\sim}0.22$ for vane shear test. By comparing the undrained shear strength estimated from DMT indices with the results measured by in-situ vane test or $CK_0U$ triaxial test, two methods to predict the undrained shear strength from DMT results are suggested. One is based on the relationship between $S_u/{\sigma}'_v$ and horizontal stress index (KD) while another method comes from $N_c-I_D$ and $N_c-E_D$ correlation. It was observed that the method based on $N_c-I_D\;or\;N_c-E_D$ relation shows slightly better accuracy than the one based on $K_D$ although all of the methods suggested in this study provided comparable values of predicted undrained shear strength. Since the definitions of $I_D\;and\;E_D$ contain $p_1-p_0$, in which soil condition is reflected, it is believed that the prediction method using $N_c$ is capable of taking a material type into consideration.

REGULARITIES OF MULTIFRACTAL HEWITT-STROMBERG MEASURES

  • Attia, Najmeddine;Selmi, Bilel
    • Communications of the Korean Mathematical Society
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    • v.34 no.1
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    • pp.213-230
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    • 2019
  • We construct new metric outer measures (multifractal analogues of the Hewitt-Stromberg measure) $H^{q,t}_{\mu}$ and $P^{q,t}_{\mu}$ lying between the multifractal Hausdorff measure ${\mathcal{H}}^{q,t}_{\mu}$ and the multifractal packing measure ${\mathcal{P}}^{q,t}_{\mu}$. We set up a necessary and sufficient condition for which multifractal Hausdorff and packing measures are equivalent to the new ones. Also, we focus our study on some regularities for these given measures. In particular, we try to formulate a new version of Olsen's density theorem when ${\mu}$ satisfies the doubling condition. As an application, we extend the density theorem given in [3].