• Title/Summary/Keyword: "The cat"

Search Result 2,110, Processing Time 0.036 seconds

Isolation of Constitutive Mutant of xylA Gene in Escherichia coli (대장균 xylA 유전자의 구성적 변이주의 분리)

  • Soh, Jae Hyun;Roh, Dong Hyun;Rhee, In Koo
    • Current Research on Agriculture and Life Sciences
    • /
    • v.11
    • /
    • pp.81-89
    • /
    • 1993
  • In order to isolate a mutant which was constitutively expressed in xylA gene, Pxyl-cat-xylA fusion gene was constructed by the insertion of cat gene between xylA promoter and xylA structural gene in pEX13 contained xylA gene. The expression of cat and xylA gene from transformants of xylA mutant DH77 with plasmid pEXC131 containing Pxyl-cat-xylA fusion gene was induced by the addition of 0.4% xylose to media. This results indicated that cat and xylA gene were expressed under control of xylA promoter the presence of xylR gene. We have also isolated constitutive mutant plasmid pEXC131-39 from pEXC131 by trementment with N-methyl-N'-nitro-N-nitrosoguanidine(NTG). cat and xylA gene from pEXC131-39 were constitutively expressed without induction of xylose regardless of xylR gene. Transformants of xylR mutant DH60 with pEXC131-39 also expressed chloramphenicol resistances and xylose isomerase without induction of xylose. This result shows that mutation in region of xylA promoter might make it possible to be constitutively expressed.

  • PDF

Analysis of Water Cycle at Main Streams in Ulsan Using CAT Model (CAT 모형을 이용한 울산지역 주요 하천유역의 물순환 분석)

  • Lee, Sang Hyeon;Cho, Hong Je
    • Journal of Korea Water Resources Association
    • /
    • v.47 no.1
    • /
    • pp.1-10
    • /
    • 2014
  • This study aimed to analyze water cycle at Taehwa river, Dongcheon, Hoiya river and Cheongryang cheon in Ulsan city using CAT model developed by Korea Institute of construction technology. To apply CAT model, we separated Teahwa river into 25, Dongcheon into 11, Hoiya river into 17 and Cheongryangcheon into 5 subbasins and discriminated between contribution runoff basins and source basins. The results of water cycle analysis performed using rainfall datas measured from 1975 and 2008 and hydrologic datas of change of land use etc. were that surface runoff increase and interflow decrease, caused by the increase of impervious area. The increases of surface runoff at the basin of Taehwa river and Dongcheon which is a tributary of Taehwa river were small and similar to each other respectively as 1.7% and 2.4%, and increased high rate of 3.2% and 7.7% in Hoiya river and Cheongryangcheon including subbasins which are having high rate of urbanization.

A Rare Case of Acute Obstructive Laryngitis in a Cat with Severe Respiratory Distress

  • Hyeona Bae;Dongbin Lee;DoHyeon Yu
    • Journal of Veterinary Clinics
    • /
    • v.40 no.2
    • /
    • pp.124-129
    • /
    • 2023
  • A 5-year-old neutered male domestic short-haired cat presented with acute dyspnea characterized by open-mouth breathing and stridor for 2 days. Direct visualization via laryngoscopy revealed diffuse laryngeal swelling and severe thickening of the vocal folds bilaterally; thus, the upper respiratory tract was obstructed owing to severe edema. Neutrophil infiltration was found on fine needle aspiration of the larynx cytology, and no discrete mass with polyp or neoplasia was identified on diagnostic imaging. The cat was diagnosed with acute obstructive laryngitis, and a tracheostomy tube was immediately installed. After 17 days of treatment with steroids, doxycycline and azithromycin, the swollen larynx gradually improved, and there was no recurrence of laryngitis or respiratory obstruction. A feline upper respiratory polymerase chain reaction panel revealed Mycoplasma felis infection; however, it could not be determined whether it was pathogenic or opportunistic. Herein, we report a case of obstructive laryngitis in a cat. When respiratory obstruction due to acute laryngitis is identified, a good prognosis is expected with rapid and appropriate treatment.

G1 the common Echinococcus granulosus genotype infected domestic cat (Felis catus) in Iraq

  • Musafer H. Al-Ardi
    • Journal of Veterinary Science
    • /
    • v.25 no.1
    • /
    • pp.7.1-7.7
    • /
    • 2024
  • Background: Infections of cats with Echinococcus granulosus is uncommon because the cat is not part of the parasite life cycle that a carnivorous and another herbivore represent. Nevertheless, it occurs incidentally when eating food or drinking water contaminated with the worm's larva, especially with the presence of the definitive host (dogs), in this case, the infections are concentrated in stray or outside cats. For this reason, this study examined the possibility of cat infection with E. granulosus and diagnosed the common genotype of this infection. Objective: This study examined the possibility of cat infection with E. granulosus and diagnosed the common genotype of this infection. Methods: Four of the 37 cats that had died in different accidents developed cystic echinococcosis (CE). The cytochrome c oxidase subunit I (COX1) gene was initially amplified and sequenced to determine if these cysts belonged to E. granulosus, in beginning. The DNA fragments resulting from sequencing were then compared and aligned with other sequences using the Gene Bank database. Finally, a phylogenetic tree was drawn according to the sequence data obtained from cox1 genes sequencing, and the MEGA 7.0 phylogenetic analysis program was utilized. Results: Four different sequences were deposited in the Gen Bank with accession numbers (ON795961 to ON795964), all of which belong to the G1 genotype. Approximately 84% and 100% of these sequences aligned with G1 (AB622277.1) and G1 (MG722980.1), respectively. Conclusions: G1 is the dominant genotype that causes cat infections, even though the cat's EC infection was incidental.

Cat Flea Infestation in a Hospital: A Case Report

  • Leelavathi, Muthupalaniappen;Norhayati, Moktar;Lee, Yin Yin
    • Parasites, Hosts and Diseases
    • /
    • v.50 no.1
    • /
    • pp.79-82
    • /
    • 2012
  • Cat flea bite in humans results in extremely pruritic skin lesions. It has been reported to occur among those living in domiciliary accommodation. However, nosocomial infestation with cat flea has not been reported. We hereby report a case of nosocomial infestation of cat flea in a hospital facility. Identification of the parasite, its appropriate eradication, and adequate medical management of the patients resulted in a satisfactory outcome.

Real-time EtherCAT Master Implementation on Xenomai for a Robot System

  • Moon, Yong-Seon;Ko, Nak-Yong;Lee, Kwang-Seok;Bae, Young-Chul;Park, Jong-Kyu
    • International Journal of Fuzzy Logic and Intelligent Systems
    • /
    • v.9 no.3
    • /
    • pp.244-248
    • /
    • 2009
  • This paper describes a real-time EtherCAT Master library. The library is developed using Xenomai. Xenomai is a real-time development framework. It cooperates with the Linux kernel, in order to provide a pervasive, interface-agnostic, hard real-time support to user-space applications, seamlessly integrated into the GNU/Linux environment. The proposed master library implements EtherCAT protocol for master side, and supports Application Programming Interfaces(APIs) for programming of real-time application which controls EtherCAT slave.

Development of Realtime EtherCAT Master Library Using INtime

  • Moon, Yong-Seon;Trong, Tuan Anh Vo;Ko, Nak-Yong;Seo, Dong-Jin;Lim, Seung-Woo
    • International Journal of Fuzzy Logic and Intelligent Systems
    • /
    • v.9 no.2
    • /
    • pp.94-98
    • /
    • 2009
  • This paper proposes an architecture of a real-time EtherCAT master library called RtEML. The controls EtherCAT slaves under EtherCAT protocol in real-time. It provides a simple programming interface which is useful in developing robot application in C/C++ or C#. To achieve deterministic, hard real-time control in Microsoft Windows environment without additional hardware, INtime is used. Since INtime is designed specifically to take advantage of the powerful capabilities of the x86 processor architecture, the proposed RtEML achieves microseconds of real-time performance.