Multiple studies conducted in the past evaluated the conductor response under one tornado wind field, while the performance of transmission lines under different tornado wind fields still remains unknown. Thus, the objective of this paper is to estimate the variation in the conductor's critical longitudinal and transverse reactions under different tornado wind fields, as well as providing the corresponding critical tornado configurations. The considered full-scale tornadoes are the Spencer, South Dakota, 1998, the Stockton, Kansas, 2005 and the Goshen County, Wyoming, 2009. Computational Fluid Dynamics (CFD) simulations were previously conducted to develop these wind fields. All tornadoes have been rescaled to have a common velocity matching the upper limit of the F2 Fujita scale. Eight conductor systems, each including six spans, are considered in this paper. For each conductor, parametric studies are conducted by varying the location of the three tornado wind fields relative to the tower of interest, therefore the peak reactions associated with each tornado are determined. A semi-analytical closed-form solution, previously developed and validated, is used to calculate the reactions. The study conducted in this paper can be divided into two parts: In the first part, a parametric study considering a wide range of tornado locations is conducted. In the second part, the parametric study focuses on the tornado location leading to the critical tangential velocity on the tower. Based on this extensive parametric study, a critical tornado defined as the Design Tornado and its critical locations, tornado distance R = 125 m, tornado angle 𝜃 = 15° and 30°, are recommended for design purposes.