The purpose of this study was to assess the effect of bridging stabilization exercises on trunk muscles activity on and off a Swiss ball. 20 healthy university students volunteered to participate in this study. Subjects were required to complete following four exercises: exercise 1, single bridging exercise; exercise 2, feet on ball bridging exercise; exercise 3, calf on ball bridging exercise; exercise 4, back on ball bridging exercise. Surface electromyography from selected trunk muscles was normalized to maximum voluntary isometric contraction. A repeated measures of ANOVA with post-hoc Bonferroni's correction was used to determine the influence of exercise type on muscle activity for each muscle and descriptive statistics was used to determine local/global muscle ratios. The rectus abdominis of exercise 4 showed significantly higher muscle activity than rectus abdominis of exercise 1, 2, 3 (p<.05). The external oblique of exercise 2, 4 showed significantly higher muscle activity than external oblique of exercise 1 (p<.05). The internal oblique of exercise 2, 4 showed significantly higher muscle activity than internal oblique of exercise 1 (p<.05). The erector spinae of exercise 2, 3, 4 showed significantly higher muscle activity than erector spinae of exercise 1 (p<.05). Median of internal oblique/rectus abdominis ratio of exercise 1 was 1.16, exercise 2 was 2.43, exercise 3 was 2.45, exercise and 4 was 1.27. Median of internal oblique/external oblique ratio of exercise 1 was 1.01, exercise 2 was .91, exercise 3 was .99, and exercise 4 was .93. Muscle activity can be influenced by addition of a Swiss ball in bridging exercises. It is recommend to use a Swiss ball for trunk stabilization exercise.