The 3D printing technology is one of the fourth industrial revolution technology that drives innovation in the manufacturing process, and should be applied to nuclear industry for various purposes according to the manufacturing trend change. In nuclear industry, it can be applied to manufacture obsolete items and new designed parts in advanced reactors or small modular reactors (SMRs), replacing the traditional manufacturing technologies. A gate valve body was manufactured, which was obsolete in nuclear power plant, using DED(Directed Energy Deposition) metal 3D printing technology after restoring design characteristics including 3D design drawing by reverse engineering. The 3D printed valve body was assembled with commercial parts such as seat-ring, disk, stem, and actuator for performance test. For the valve assembly, including 3D printed valve body, several tests were performed, including pressure test, end-loading test, and seismic test according to KEPIC MGG and KEPIC MFC. In the pressure test, hydraulic pressure of 391kgf/cm2 was applied to 3D printed valve body, and no leak was detected. Also the 3D printed valve assembly was performed well in end-loading and seismic tests.