At present, quarternary ammonium salts(cationic surfactant) and Tego compounds (amphoteric surfactant) are used as germicidal agent. In this paper, it was investigated whether their germicidal activities are influenced or not by some suspending agents which are added to them 0.005% benzalkonium chloride aq. solution and 0.05% dodecyl diaminoethyl glycine aq. solution sterilized respectively against Staphylococcus aureus and Escherichia coli within a minute. The solutions aded acacia, carboxymethyl cellulose, sodium alginate solutions to make to 0.005% to above surfactants solution decreased the germicidal activity, not being sterilized bacteria for more than 2 hours however, the solutions decreased the germicidal activity by addition of suspending agents such as acacia, carboxymethyl cellose, sodium alginate and bacteria were survival for more than 2 hrs, 0.05% of benzalkonium chloride and 0.1% dodecyl diaminoethyl glycine respectively would be sterilized within 10 minutes, when added to 5% suspending agents. This result show that bactericidal action of dodecyl diaminoethyl glycine is affected by suspending agents less than that of benzalkonium chloride.