Echocardiography is a widely used test to evaluate the contractility of the heart. However, this exam is cannot be said to be objective because the test relies on the examiner's visual judgment. Therefore, although a lot of efforts are being made to quantify the strain through speckle tracking echocardiography (STE), there are some limitations, and studies of conditions to supplement are needed. Through this study, we determine whether the general characteristics of the subjects affect vessel-specific strain and whether changes in various imaging conditions (general, resolution, compression, gain, chroma) affect vessel-specific strain. According to the results of the study, the general conditions obtained during the examination were generally less significant compared to other conversion conditions, and Resolution, Compress, Gain, Chroma, and LAD showed significant correlations under the Compress conditions (p < .05). Therefore, Accurate strain assessment will be made if echocardiography is analyzed using significant conditions for each stenotic vessel