Journal of Microbiology and Biotechnology

  • 제34권1호
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  • Pages.167-175
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  • 2024
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Isolation of a Potential Probiotic Levilactobacillus brevis and Evaluation of Its Exopolysaccharide for Antioxidant and α-Glucosidase Inhibitory Activities

  • Se-Young Kwun (Department of Food Biotechnology and Environmental Science, Kangwon National University) ;
  • Jeong-Ah Yoon (Department of Food Biotechnology and Environmental Science, Kangwon National University) ;
  • Ga-Yeon Kim (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Young-Woo Bae (Department of Food Biotechnology and Environmental Science, Kangwon National University) ;
  • Eun-Hee Park (Department of Food Science and Biotechnology, Kangwon National University) ;
  • Myoung-Dong Kim (Department of Food Science and Biotechnology, Kangwon National University)
  • 투고 : 2023.04.25
  • 심사 : 2023.09.29
  • 발행 : 2024.01.28
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초록

The probiotic properties of ten lactic acid bacteria and antioxidant and α-glucosidase inhibitory activities of the exopolysaccharide (EPS) of the selected strain were investigated in this study. Levilactobacillus brevis L010 was one of the most active strains across all the in vitro tests. The cell-free supernatant (50 g/l) of L. brevis L010 showed high levels of both α-glucosidase inhibitory activity (98.73 ± 1.32%) and 2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging activity (32.29 ± 3.86%). The EPS isolated from cell-free supernatant of L. brevis L010 showed 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) radical-scavenging activity (80.27 ± 2.51%) at 80 g/l, DPPH radical-scavenging activity (38.19 ± 9.61%) at 40 g/l, and ferric reducing antioxidant power (17.35 ± 0.20 mg/l) at 80 g/l. Further, EPS exhibited inhibitory activities against α-glucosidase at different substrate concentrations. Kinetic analysis suggests that the mode of inhibition was competitive, with a kinetic constant of Km = 2.87 ± 0.88 mM and Vmax = 0.39 ± 0.06 μmole/min. It was concluded that the EPS might be one of the plausible candidates for possible antioxidant and α-glucosidase activities of the L. brevis L010 strain.

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과제정보

This research was financially supported by the Ministry of Trade Industry and Energy, Korea, under the "Regional Specialized Industry Development Program" (reference number P0002815) supervised by the Korea Institute for Advancement of Technology. This research was funded by the Research Program for Agricultural Science and Technology Development (Project No. RS-2022-RD010225) and the National Institute of Agricultural Science, Rural Development Administration, Republic of Korea.

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