Journal of Microbiology and Biotechnology

  • 제34권1호
  • /
  • Pages.65-73
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  • 2024
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
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Skin Barrier-Enhancing Effects of Dermabiotics HDB with Regulation of Skin Microbiota

  • Kyung Min Kim (Biohealthcare R&D Center, HYUNDAI BIOLAND Co., Ltd.) ;
  • Ji-Won Song (Biohealthcare R&D Center, HYUNDAI BIOLAND Co., Ltd.) ;
  • Chang-Wan Lee (Biohealthcare R&D Center, HYUNDAI BIOLAND Co., Ltd.) ;
  • Du-Seong Kim (Biohealthcare R&D Center, HYUNDAI BIOLAND Co., Ltd.) ;
  • Johann Sohn (Biohealthcare R&D Center, HYUNDAI BIOLAND Co., Ltd.) ;
  • Seunghun Lee (Biohealthcare R&D Center, HYUNDAI BIOLAND Co., Ltd.)
  • 투고 : 2023.06.26
  • 심사 : 2023.09.19
  • 발행 : 2024.01.28
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초록

In the regulation of inflammatory responses and skin homeostasis, the skin and its microbiota are closely related. Studies have reported that lactic acid bacteria extracts can improve the skin condition and microbiota. In our previous study, we developed probiotic lysates, which are efficacious in improvement of human skin cells and the skin barrier. The skin-moisturizing effect of Dermabiotics HDB (HDB) prepared with Lactiplantibacillus plantarum, and the correlation between changes in the skin microbiota and moisture contents, were evaluated and analyzed in clinical trials. The clinical parameters on the cheeks of 21 female participants were measured using biophysical tools before and after (2 weeks) using HDB or control. The skin microbes were collected and identified using 16s rRNA gene sequencing. HDB significantly improved moisture intensity, transepidermal water loss (TEWL), and hot flush level on the cheek. The beta-diversity of the skin microbiota was different from that of the control in the unweighted UniFrac principal coordinate analysis after using HDB. The genus Lawsonella demonstrated a positive correlation with TEWL and a negative correlation with the moisture contents of the keratin layer, regardless of the use of HDB and control. Conversely, after HDB use, the genus Staphylococcus was increased and associated with a lower hot flush level, while the genera of the phylum Proteobacteria tended to decrease, which is associated with an improved skin condition. Overall, HDB showed clinically proven effects, including skin moisturization with regulation of the skin microbiota.

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참고문헌

  1. Oh J, Byrd AL, Deming C, Conlan S, NISC, Kong HH. 2014. Biogeography and individuality shape function in the human skin metagenome. Nature 514: 59-64. 
  2. Grice EA, Segre JA. 2011. The skin microbiome. Nat. Rev. Microbiol. 9: 224-253. 
  3. Yang Y, Qu L, Mijakovic I, Wei Y. 2022. Advances in the human skin microflora and its roles in cutaneous diseases. Microb. Cell Fact. 21: 176. 
  4. Rozas M, Ruijter AH, Fabrega MJ, Zorgani A, Guell M, Paetzold B, et al. 2021. From dysbiosis to healthy skin: major contributions of Cutibacterium acnes to skin homeostasis. Microorganisms 9: 628. 
  5. Fourniere M, Latire L, Souak D, Feuilloley M, Bedous G. 2020. Staphylococcus epidermidis and Cutibacterium acnes: two major sentinels of skin microflora and the influence of cosmetics. Microorganisms 8: 1752. 
  6. Francuzik W, Frankek K, Schumann RR, Heine G, Worm M. 2018. Propionibacterium acnes abundance correlates inversely with Staphylococcus aureus: data from atopic Ddermatitis skin microbiome. Acta Derm. Venereol. 98: 490-495. 
  7. Skowron K , Bauza-Kaszewska J, Kraszewska Z, Wiktorczyk-Kapischke N, Grudelwska-Buda K, Keiwcinska-Pirog J, et al. 2021. Human skin microbiome: impact of intrinsic and extrinsic factors on skin microflora. Microorganisms 9: 543. 
  8. Lim HY, Jeong D, Park SH, Shin KK, Hong YH, Kim E, et al. 2020. Antiwrinkle and antimelanogenesis effects of tyndallized Lactobacillus acidophilus KCCM12625P. Int. J. Mol. Sci. 21: 1620. 
  9. Jung YO, Jeong H, Cho Y, Lee EO, Jang HW, Kim J, et al. 2019. Lysates of a probiotic, Lactobacillus rhamnosus, can improve skin barrier function in a reconstructed human epidermis model. Int. J. Mol. Sci. 20: 4289. 
  10. Khmaladze I, Butler E, Fabre S, Gillbro J. 2019. Lactobacillus reuteri DSM 17938-A comparative study on the effect of probiotics and lysates on human skin. Exp. Dermatol. 28: 822-828. 
  11. Kim HJ, Oh HN, Park T, Kim H, Lee HG, An S, et al. 2022. Aged related human skin microbiome and mycobiome in Korean women. Sci. Rep. 12: 2351. 
  12. Yang SJ, Lee CW, Cha SY, Chio JW, Lee S. 2021. Skin barrier enhancement of ferment using lava seawater and Lactobacillus plantarum HDB1234 as a novel cosmetic ingredient. J. Kor. Soc. Cosmetol. 27: 356-363. 
  13. Parada, AE, Needham DM, Fuhrman JA. 2016. Every base matters: assessing small subunit rRNA primers for marine microbiomes with mock communities, time series and global field samples. Environ. Microbiol. 18: 1403-1414. 
  14. Rognes T, Flouri T, Nichols B, Quince C, Mahe F. 2016. VSEARCH: a versatile open source tool for metagenomics. PeerJ. 4: e2584. 
  15. Caporaso JG, Kuczynski J, Stombaugh J, Bitteinger K, Bushman FD, Kostello EK, et al. 2010. QIIME allows analysis of high-throughput community sequencing data. Nat. Methods 7: 335. 
  16. Lee HJ, Jeong SE, Lee S, Kim S, Han H, Jeon CO. 2018. Effects of cosmetics on the skin microbiome of facial cheeks with different hydration levels. Microbiology 7: e00557. 
  17. Ogai K, Nagase S, Mukai K, Luchi T, Mori Y, Matsue M, et al. 2018. A comparison of techniques for collecting skin microbiome samples: swabbing versus tape-stripping. Front. Microbiol. 2: 2362. 
  18. Kim JH, Son SM, Park H, Kim BK, Choi IS, Kim H, et al. 2021. Taxonomic profiling of skin microbiome and correlation with clinical skin parameters in healthy Koreans. Sci. Rep. 11: 16269. 
  19. Kim HS. 2020. Microbiota in Rosacea. Am. J. Clin. Dermatol. 21: S25-S35. 
  20. Callewaert C, Helffer KR, Lebaron P. 2020. Skin microbiome and its interplay with the environment. Am. J. Clin. Dermatol. 21: 4-11. 
  21. Grice EA, Kong HH, Sean Conlan S, Deming CB, Davis J, Young AC, et al. 2009. Topographical and temporal diversity of the human skin microbiome. Science 324: 1190-1192.