Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Weighted gene co-expression network analysis identifies important modules and hub genes involved in the regulation of breast muscle yield in broilers

  • Xing Guo (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Hao Wang (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Meng Liu (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Jin-Mei Xu (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Ya-Nan Liu (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Hong Zhang (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Xin-Xin He (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Jiang-Xian Wang (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Wei Wei (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Da-Long Ren (College of Animal Science and Technology, Anhui Agricultural University) ;
  • Run-Shen Jiang (College of Animal Science and Technology, Anhui Agricultural University)
  • Received : 2023.12.28
  • Accepted : 2024.03.10
  • Published : 2024.10.01
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Abstract

Objective: Increasing breast meat production is one of the primary goals of the broiler industry. Over the past few decades, tremendous progress has been made in genetic selection and the identification of candidate genes for improving the breast muscle mass. However, the molecular network contributing to muscle production traits in chickens still needs to be further illuminated. Methods: A total of 150 1-day-old male 817 broilers were reared in a floor litter system. At the market age of 50 d, eighteen healthy 817 broilers were slaughtered and the left pectoralis major muscle sample from each bird was collected for RNA-seq sequencing. The birds were then plucked and eviscerated and the whole breast muscle was removed and weighed. Breast muscle yield was calculated as the ratio of the breast muscle weight to the eviscerated weight. To identify the co-expression networks and hub genes contributing to breast muscle yield in chickens, we performed weighted gene co-expression network analysis (WGCNA) based on the 18 transcriptome datasets of pectoralis major muscle from eighteen 817 broilers. Results: The WGCNA analysis classified all co-expressed genes in the pectoral muscle of 817 broilers into 44 modules. Among these modules, the turquoise and skyblue3 modules were found to be most significantly positively (r = 0.78, p = 1e-04) and negatively (r = -0.57, p = 0.01) associated with breast meat yield, respectively. Further analysis identified several hub genes (e.g., DLX3, SH3RF2, TPM1, CAV3, MYF6, and CFL2) that involved in muscle structure and muscle development were identified as potential regulators of breast meat production. Conclusion: The present study has advanced our understanding of the molecular regulatory networks contributing to muscle growth and breast muscle production and will contribute to the molecular breeding of chickens in the future.

Keywords

Acknowledgement

This work was supported by the Science and Technology Major Project of Anhui Province (202203a06020015); the key research and development project of Anhui Province (202204c06020050); the Science and Technology Major Project of Huaibei city (HK2021015); the Major Special science and technology project of Anhui province (202103b06020023); the Natural Science Research Project of Anhui Educational Committee (KJ2021A0148); and the China Agriculture Research System of MOF and MARA (CARS-41).

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