Animal Bioscience

Asian Australasian Association of Animal Production Societies (아세아태평양축산학회)
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Association of histamine-N-methyl transferase gene polymorphisms with carnosine content in red-brown Korean native chickens

  • Jean Pierre Munyaneza (Division of Animal and Dairy Science, Chungnam National University) ;
  • Minjun Kim (Division of Animal and Dairy Science, Chungnam National University) ;
  • Eunjin Cho (Department of Bio-AI Convergence, Chungnam National University) ;
  • Aera Jang (Department of Applied Animal Science, College of Animal Life Science, Kangwon National University) ;
  • Hyo Jun Choo (Poultry Research Institute, National Institute of Animal Science) ;
  • Jun Heon Lee (Division of Animal and Dairy Science, Chungnam National University)
  • Received : 2023.12.29
  • Accepted : 2024.03.14
  • Published : 2024.09.01
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Abstract

Objective: Carnosine and anserine affect the meat flavor. The contents of carnosine and anserine in meat are affected by genetic and environmental factors. This study aimed to discover the single-nucleotide polymorphisms (SNPs) in the histamine-N-methyl transferase (HNMT) and histamine-N-methyl transferase-like (HNMT-like) genes and to associate them with the content of carnosine and anserine in Korean native chicken-red brown line (KNC-R). Methods: This study used a total of 384 birds (males, n = 192; females, n = 192) aged 10 weeks old, for genotyping HNMT and HNMT-like genes. One synonymous SNP (rs29009298C/T) of the HNMT gene was genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) methods whereas four missense SNPs (rs734406537G/A; rs736514667A/G; rs15881680G/A and rs316765035T/C) of the HNMT gene, and one missense SNP rs737657949A/C of the HNMT-like gene were genotyped by PCR allele competitive extension (PACE) genotyping technology. Two-way analysis of variance of the R program was used to associate HNMT genotypes with the contents of carnosine and anserine in KNC-R chickens. Results: There were significant associations (p<0.05) between the genotypes of the synonymous SNP:rs29009298C/T, missense SNP rs736514667A/G of the HNMT gene and the content of carnosine in KNC-Rs. This study also reported the sex effect on the carnosine content, where females had more content of carnosine compared to that of male KNC-R. Conclusion: Two SNPs (synonymous: rs735769522C/T) and missense: rs736514667A/G) in the HNMT gene might be used as genetic markers in the selection and breeding of chickens with better taste and high-flavored meat.

Keywords

Acknowledgement

This study was financially supported by the project funding number: RS-2021-RD010125 (PJ016205) of the Rural Development Administration, South Korea.

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