Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Synergistic Antibacterial Effect of Eisenia bicyclis Extracts in Combination with Antibiotics against Fish Pathogenic Bacteria

  • Raul Joao Lourenco Mascarenha (KOICA-PKNU International Graduate Program of Fisheries Science, Pukyong National University) ;
  • Du-Min Jo (National Marine Biodiversity Institute of Korea) ;
  • Yoon-Ah Sim (Department of Food Science and Technology, Pukyong National University) ;
  • Do-Hyung Kim (Department of Aquatic Life Medicine, Pukyong National University) ;
  • Young-Mog Kim (KOICA-PKNU International Graduate Program of Fisheries Science, Pukyong National University)
  • Received : 2024.06.15
  • Accepted : 2024.07.02
  • Published : 2024.10.28
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Abstract

The aquaculture industry faces significant challenges due to bacterial infections caused by Edwardsiella tarda, Photobacterium damselae, and Vibrio harveyi. The extensive use of traditional antibiotics, has resulted in widespread antibiotic resistance. This study aimed to investigate the antibacterial potential of the brown seaweed Eisenia bicyclis, particularly its synergistic effects with antibiotics against these fish pathogenic bacteria. E. bicyclis were processed to obtain methanolic extracts and fractionated using different polar solvents. The antibacterial activities of these extracts and fractions were assessed through disc diffusion and minimum inhibitory concentration (MIC) assays. The study further evaluated the antibiotic susceptibility of the bacterial strains and the synergistic effects of the extracts combined with erythromycin and oxyteteracycline using the fractional inhibitory concentration index. Results showed that the ethyl acetate (EtOAc) fraction of E. bicyclis methanolic extract exhibited the highest antibacterial activity. The combination of the EtOAc fraction with erythromycin significantly enhanced its antibacterial efficacy against the tested strains. This synergistic effect was indicated by a notable reduction in MIC values, demonstrating the potential of E. bicyclis to enhance the effectiveness of traditional antibiotics. The findings suggest that E. bicyclis extracts, particularly the EtOAc fraction, could serve as a potent natural resource to counteract antibiotic resistance in aquaculture.

Keywords

Acknowledgement

This research was supported by the Korea Institute of Marine Science & Technology Promotion (KIMST) and funded by the Ministry of Oceans and Fisheries (RS-2024-00404977).

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