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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Rice CHD3/Mi-2 chromatin remodeling factor RFS regulates vascular development and root formation by modulating the transcription of auxin-related genes NAL1 and OsPIN1

  • Hyeryung Yoon (Department of Agriculture, Forestry and Bioresources, Plant Genomics and Breeding Institute, Research Institute of Agriculture and Life Sciences, Seoul National University) ;
  • Chaemyeong Lim (Department of Agriculture, Forestry and Bioresources, Plant Genomics and Breeding Institute, Research Institute of Agriculture and Life Sciences, Seoul National University) ;
  • Bo Lyu (Division of Plant Science and Technology, University of Missouri) ;
  • Qisheng Song (Division of Plant Science and Technology, University of Missouri) ;
  • So-Yon Park (Division of Plant Science and Technology, University of Missouri) ;
  • Kiyoon Kang (Division of Life Sciences, Incheon National University) ;
  • Sung-Hwan Cho (Department of Agriculture, Forestry and Bioresources, Plant Genomics and Breeding Institute, Research Institute of Agriculture and Life Sciences, Seoul National University) ;
  • Nam-Chon Paek (Department of Agriculture, Forestry and Bioresources, Plant Genomics and Breeding Institute, Research Institute of Agriculture and Life Sciences, Seoul National University)
  • Received : 2024.01.13
  • Accepted : 2024.02.23
  • Published : 2024.10.31
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Abstract

The vascular system in plants facilitates long-distance transportation of water and nutrients through the xylem and phloem, while also providing mechanical support for vertical growth. Although many genes that regulate vascular development in rice have been identified, the mechanism by which epigenetic regulators control vascular development remains unclear. This study found that Rolled Fine Striped (RFS), a Chromodomain Helicase DNA-binding 3 (CHD3)/Mi-2 subfamily protein, regulates vascular development in rice by affecting the initiation and development of primordia. The rfs mutant was found to affect auxin-related genes, as revealed by RNA sequencing and reverse transcription-quantitative PCR analysis. The transcript levels of OsPIN1 and NAL1 genes were downregulated in rfs mutant, compared to the wild-type plant. The chromatin immunoprecipitation assays showed lower levels of H3K4me3 in the OsPIN1a and NAL1 genes in rfs mutant. Furthermore, exogenous auxin treatment partially rescued the reduced adventitious root vascular development in rfs mutant. Subsequently, exogenous treatments with auxin or an auxin-transport inhibitor revealed that the expression of OsPIN1a and NAL1 is mainly affected by auxin. These results provide strong evidence that RFS plays an important role in vascular development and root formation through the auxin signaling pathway in rice.

Keywords

Acknowledgement

This research was supported by the Basic Science Research Program through the NRF of Korea grant funded by the Korean Government (NRF-2010-355-F00003), the Brain Pool program funded by the Ministry of Science and ICT through the National Research Foundation of Korea (2022H1D3A2A01096185), and the Basic Science Research Program through the NRF of Korea grant funded by the Korean Government (RS-2023-00247376).

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