The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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A comparative study on enhanced anti-inflammatory activity of Cannabis root extract prepared by combined processing process

복합가공 과정에 의한 대마 뿌리 추출물의 항염증 활성 증진에 관한 비교 연구

  • Ga-Ram Yu (Department of Diagnostics, College of Korean Medicine, Dongguk University ) ;
  • Hye-Lin Jin (Department of Diagnostics, College of Korean Medicine, Dongguk University ) ;
  • Da-Hoon Kim (TOPO Lab., Co., Ltd.) ;
  • Jay Kyun Noah (The Korean Cannabis Industry Association) ;
  • Hyuck Kim (Department of Diagnostics, College of Korean Medicine, Dongguk University ) ;
  • Dong-Woo Lim (Department of Diagnostics, College of Korean Medicine, Dongguk University )
  • 유가람 (동국대학교 한의과대학 진단학교실) ;
  • 진혜린 (동국대학교 한의과대학 진단학교실) ;
  • 김다훈 (토포랩 주식회사) ;
  • 노중균 (사단법인 한국대마산업협회) ;
  • 김혁 (동국대학교 한의과대학 진단학교실) ;
  • 임동우 (동국대학교 한의과대학 진단학교실)
  • Received : 2024.04.15
  • Accepted : 2024.05.07
  • Published : 2024.06.01
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Abstract

Objectives: Cannabis root is a medicinal plant that has been used in traditional medicine around the world. However, in previous studies using simple extracts, the biological activity was not relatively prominent compared to other herbal medicines. The aim of the present study is to confirm the enhancement of anti-inflammatory activity and bioactive compound of Cannabis root extract prepared by combined processing process. Methods: A series of processes including repeated steam and dry, fermentation, hydrothermal extraction and ethanol extraction was applied to Cannabis root. The antioxidant content of cannabis root extracts obtained through a combined processing process was investigated by analyzing the total phenolic, tannin, and flavonoid contents. Anti-inflammatory effects were tested in LPS-treated RAW264.7 cells. The anti-inflammatory mechanism was examined by western blot. Finally, the component profile of Cannabis root extract was analyzed using High Performance Liquid Chromatography (HPLC) and Thin layer chromatography (TLC). Results: The cannabis root extract prepared by complex processing process had higher antioxidant and anti-inflammatory effects than simple extract. Total phenolic and tannin contents were significantly increased, and DPPH free radical inhibition activity was strengthened by combined processing process. Increased NO production and iNOS expression in LPS-treated RAW264.7 cells were decreased in a concentration-dependent manner upon extract treatment by complex processing process. Additionally, the Stigmasterol content of Cannabis root extract was increased through a complex processing process. Conclusions: Further research is needed on the mechanisms and substances that exhibit the anti-inflammatory effects of Cannabis roots extract prepared by complex processing process.

Keywords

Acknowledgement

이 논문은 2023년도 정부(교육부)의 재원으로 한국연구재단의 지원을 받아 수행된 기초연구사업임(No. 2022R1A6A3A01087626), 한국의료대마운동본부 상임고문 김성복 목사님의 노고에 의해 결실을 맺게 되었으므로 감사의 말씀을 드립니다.

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