대한의생명과학회지 (Biomedical Science Letters)

  • 제30권3호
  • /
  • Pages.113-122
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  • 2024
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  • 1738-3226(pISSN)
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  • 2288-7415(eISSN)
대한의생명과학회 (The Korean Society for Biomedical Laboratory Sciences)
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Anti-Cancer Effects of the Pandanus tectorius Parkinson Extract: Reduction of YAP and TAZ Levels via Inhibition of the Hippo and Notch Signaling Pathways

  • Min Kyu Kang (Department of Biomedical Laboratory Science, Daegu Haany University) ;
  • Da Hyun Kim (Department of Biomedical Laboratory Science, Kyungbok University)
  • 투고 : 2024.05.31
  • 심사 : 2024.08.08
  • 발행 : 2024.09.30
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초록

In this study, we aimed to investigate the anti-cancer effects of the Pandanus tectorius extract on AGS cells. P. tectorius, commonly known as hala or screw pine, is a tropical plant traditionally used for its medicinal properties, including anti-inflammatory and antioxidant properties. Here, effects of the P. tectorius extract on cell proliferation, migration, and gene expression were evaluated using various assays, including the water-soluble tetrazolium salt (WST)-1, wound healing, migration, and western blotting assays. WST-1 assay revealed a significant dose- and time-dependent decrease in cell viability, with higher concentrations of the extract resulting in more pronounced viability inhibition. Wound healing and migration assays revealed that the P. tectorius extract effectively hindered cell migration, as the treated cells showed considerably slower wound closure and reduced migration than the control cells. Molecular analysis revealed that the extract significantly downregulated the expression levels of key oncogenic proteins, genes, and components of the Notch signaling pathway. Western blotting confirmed the substantial reduction in the marker protein levels in treated cells. These findings suggest that P. tectorius extract exerts its anti-cancer effects by inhibiting multiple signaling pathways crucial for cancer cell proliferation, migration, and survival. Overall, this study highlights the potential of P. tectorius extract as a therapeutic agent for gastric cancer treatment.

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참고문헌

  1. Anirudhan A, Iryani MTM, Andriani Y, et al. The effects of Pandanus tectorius leaf extract on the resistance of white-leg shrimp penaeus vannamei towards pathogenic vibrio parahaemolyticus. Fish and Shellfish Immunololgy Reports. 2023. 4: 100101.
  2. BeLow M, Osipo C. Notch signaling in breast cancer: A role in drug resistance. Cells. 2020. 9.
  3. Benjakul N, Prakobphol N, Tangshewinsirikul C, et al. Notch signaling regulates vasculogenic mimicry and promotes cell morphogenesis and the epithelial-to-mesenchymal transition in pancreatic ductal adenocarcinoma. PLoS One. 2022. 17: e0279001.
  4. Cheng C, Park SC, Giri SS. Effect of Pandanus tectorius extract as food additive on oxidative stress, immune status, and disease resistance in cyprinus carpio. Fish and Shellfish Immunology. 2022. 120: 287-294.
  5. Cheng F, Wang J, Wang R, et al. Fgf2 promotes the proliferation of injured granulosa cells in premature ovarian failure via hippo-yap signaling pathway. Molecular and Cellular Endocrinology. 2024. 589: 112248.
  6. Del Mundo CR, Castillo AL, An SSA, Tan MA. In vivo cox-2 modulation and metabolite profiling of Pandanus tectorius leaves extracts. 3 Biotech. 2020. 10: 90.
  7. Fonseca T, Coimbra M, Santos-Sousa H, Barbosa E, Barbosa J. Impact of perioperative chemotherapy in the treatment of patients with gastric cancer. Porto Biomedical Journal. 2022. 7: e180.
  8. Ghazvinian Z, Abdolahi S, Ahmadvand M, et al. Chemo-immune cell therapy by intratumoral injection of adoptive nk cells with capecitabine in gastric cancer xenograft model. Bioimpacts. 2023. 13: 383-392.
  9. Giacomini A, Taranto S, Rezzola S, et al. Inhibition of the fgf/fgfr system induces apoptosis in lung cancer cells via c-myc downregulation and oxidative stress. International Journal of Molecular Science. 2020. 21.
  10. Heng BC, Zhang X, Aubel D, et al. Role of yap/taz in cell lineage fate determination and related signaling pathways. Frontiers in Cell and Developmental Biology. 2020. 8: 735.
  11. Jin WM, Zhu Y, Cai ZQ, et al. Progress of clinical studies targeting claudin18.2 for the treatment of gastric cancer. Digestive Diseases and Sciences. 2024. 2631-2647.
  12. Jin Y, Qiu J, Lu X, Li G. C-myc inhibited ferroptosis and promoted immune evasion in ovarian cancer cells through ncoa4 mediated ferritin autophagy. Cells. 2022. 11.
  13. Kaur S, Najm MZ, Khan MA, et al. Drug-resistant breast cancer: Dwelling the hippo pathway to manage the treatment. Breast Cancer (Dove Med Press). 2021. 13: 691-700.
  14. Kim DH, Lee HW, Park HW, Lee HW, Chun KH. Bee venom inhibits the proliferation and migration of cervical-cancer cells in an hpv e6/e7-dependent manner. BMB Reports. 2020a. 53: 419-424.
  15. Kim DH, Lee S, Kang HG, Chun KH et al. Synergistic antitumor activity of a dll4/vegf bispecific therapeutic antibody in combination with irinotecan in gastric cancer. BMB Reports. 2020b. 53: 533-538.
  16. Kim DH, Yang EJ, Lee JA, Chang JH. Ginkgo biloba leaf extract regulates cell proliferation and gastric cancer cell death. Korean Journal of Biochemistry and Molecular Biology. 2022. 28: 92-100.
  17. Kim JW, Ko JH, Sage J. Dll3 regulates notch signaling in small cell lung cancer. iScience. 2022. 25: 105603.
  18. Linhares M, Pinto CM, Libanio D, Teixeira MR, Dinis-Ribeiro M, Brandao C. Gastric cancer: A practical review on management of individuals with hereditary or familial risk for gastric cancer. GE Portuguese Journal of Gastroenterology. 2023. 30: 253-266.
  19. Lo Sardo F, Pulito C, Sacconi A, et al. Yap/taz and ezh2 synergize to impair tumor suppressor activity of tgfbr2 in non-small cell lung cancer. Cancer Letters. 2021. 500: 51-63.
  20. Miliotis C, Ma Y, Katopodi XL, et al. Determinants of gastric cancer immune escape identified from non-coding immune-landscape quantitative trait loci. Nature Communications. 2024. 15: 4319.
  21. Pan C, Hao X, Deng X, et al. The roles of hippo/yap signaling pathway in physical therapy. Cell Death Discovery. 2024. 10: 197.
  22. Riffet M, Eid Y, Faisant M, et al. Deciphering promoter hyper-methylation of genes encoding for rassf/hippo pathway reveals the poor prognostic factor of rassf2 gene silencing in colon cancers. Cancers (Basel). 2021. 13.
  23. Tyagi A, Sharma AK, Damodaran C. A review on notch signaling and colorectal cancer. Cells. 2020. 9.
  24. Wu CM, Luan H, Wang S, Zhang XP, Liu HT, Guo P. Pandanus tectorius derived caffeoylquinic acids inhibit lipid accumulation in hepg2 hepatoma cells through regulation of gene expression involved in lipid metabolism. Yao Xue Xue Bao. 2015. 50: 278-283.
  25. Xia R, Xu M, Yang J, Ma X. The role of hedgehog and notch signaling pathway in cancer. Molecular Biomedicine. 2022. 3: 44.
  26. Yousefi H, Bahramy A, Zafari N, et al. Notch signaling pathway: A comprehensive prognostic and gene expression profile analysis in breast cancer. BMC Cancer. 2022. 22: 1282.
  27. Yuan Y, Park J, Feng A, Awasthi P, Wang Z, Chen Q, Iglesias-Bartolome R. Yap1/taz-tead transcriptional networks maintain skin homeostasis by regulating cell proliferation and limiting klf4 activity. Nature Communications. 2020. 11: 1472.
  28. Zakiryanova GK, Kustova E, Urazalieva NT, et al. Notch signaling defects in nk cells in patients with cancer. Cancer Immunology Immunotherapy. 2021. 70: 981-988.
  29. Zhu X, Ge B, Wen L, Huang H, Shi X. Analysis of multiple factors influencing the survival of patients with advanced gastric cancer. Aging. 2024. 16.