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Effect of Expanded Polystyreneon Growth and Development Time of Tenebrio molitor

발포폴리스티렌이 Tenebrio molitor의 성장 및 발달 기간에 미치는 영향

  • Ji Yun Yun (Department of Horticulture, Kongju National University) ;
  • Se Min Lee (Department of Horticulture, Kongju National University) ;
  • Soo Jin Jeong (Department of Horticulture, Kongju National University) ;
  • Ava Johnston (Department of Mathematics and Statistics, California State University) ;
  • Emelyn Anderson (Department of Mathematics and Statistics, California State University) ;
  • Steven Kim (Department of Mathematics and Statistics, California State University) ;
  • Dong Sub Kim (Department of Horticulture, Kongju National University)
  • 윤지윤 (공주대학교 산업과학대학 원예학과 ) ;
  • 이세민 (공주대학교 산업과학대학 원예학과 ) ;
  • 정수진 (공주대학교 산업과학대학 원예학과 ) ;
  • 아바 존스톤 (캘리포니아 주립대학교 몬트레이베이 수학통계학과 ) ;
  • 에멜린 안데르손 (캘리포니아 주립대학교 몬트레이베이 수학통계학과 ) ;
  • 스티븐 김 (캘리포니아 주립대학교 몬트레이베이 수학통계학과 ) ;
  • 김동섭 (공주대학교 산업과학대학 원예학과 )
  • Received : 2024.07.26
  • Accepted : 2024.07.31
  • Published : 2024.07.31

Abstract

Many studies have reported that mealworms can ingest plastic during their larval stage. The purpose of this study was to investigate the effects of expanded polystyrene consumption on the growth and development of mealworms during their larval stage. We found that the growth rate is significantly different between mealworms consumed wheat bran and mealworms consumed expanded polystyrene (p < 0.001). The transformation into pupa occurred faster among the mealworms consumed expanded polystyrene than those consumed wheat bran (p < 0.001). However, the survival rate was not significantly different between the two groups of mealworms (p = 0.786). Based on the data, we conclude that mealworms consuming expanded polystyrene have a slower weight gain and a shorter development period than mealworms consuming wheat bran, but the expanded polystyrene does not affect the survival rate before the transformation into pupa. Practically, we anticipate that mealworms can be a resource for the sustainable and eco-friendly removal of expanded polystyrene waste.

많은 연구에 따르면 Tenebrio molitor은 유충 단계에서 플라스틱을 섭취할 수 있다고 보고되었다. 이 연구의 목적은 T. molitor 유충의 성장과 발달에 발포폴리스티렌 섭취가 미치는 영향을 조사하는 것이다. 밀기울을 섭취한 유충의 성장률은 발포폴리스티렌을 섭취한 유충의 성장률보다 더 좋았고(p < 0.001) 발포폴리스티렌을 섭취한 유충의 번데기로 전환되는 기간은 밀기울을 섭취한 유충의 번데기로 전환되는 기간보다 더 빨랐다(p < 0.001). 하지만 두 처리구간 생존율은 유의미한 차이가 없었다(p = 0.786). 이 결과에 따르면 발포폴리스티렌을 섭취한 유충은 체중 감소와 짧은 발육기간이 특징이지만 생존하는 것에는 문제가 없었다. 따라서 우리는 T. molitor가 플라스틱 폐기물의 지속 가능하고 친환경적인 제거를 위한 주요 자원이라는 결론을 내렸다.

Keywords

References

  1. Bates D., M. Maechler, B. Bolker, and S. Walker 2015, Fitting linear mixed-effects models using lme4. J Stat Softw 67(1):1-48. doi:10.18637/jss.v067.i01
  2. Geyer R., J.R. Jambeck, and K.L. Law 2017, Production, use, and fate of all plastics ever made. Sci Adv 3(7):e1700782. doi:10.1126/sciadv.1700782
  3. Gu B.J. 2023, Fine Structural study on the microplastic biodegradation in the digestive tract of the mealworm, Tenebrio molitor. master's thesis, Dankook Univ. (in Korean)
  4. Hong S.Y., C.W. Lee, S.W. Hong, J.M. Lee, and Y.C. Jang 2014, Evaluation of beach pollution by aquaculture Styrofoam buoys in Tongyeong, Korea. J Korean Soc Mar Environ Energy 17(2):104-115. doi:10.7846/JKOSMEE.2014.17.2.104
  5. Kuznetsova A., P.B. Brockhoff, and R.H.B. Christensen 2017, lmerTest package: Tests in linear mixed effects models. J Stat Softw 82(13):1-26. doi:10.18637/jss.v082.i13
  6. Lebreton L., M. Egger, and B. Slat 2019, A global mass budget for positively buoyant macroplastic debris in the ocean. Sci Rep 9(1):12922. doi:10.1038/s41598-019-49413-5
  7. Ministry of Environment in South Korea 2017, The 5th National Waste Statistics Survey. (in Korean)
  8. Mealworm Nara (in Korean), https://mwnara.co.kr/ Accessed 12 March 2024.
  9. Nukmal N., S. Umar, S.P. Amanda, and M. Kanedi 2018, Effect of Styrofoam waste feeds on the growth, development and fecundity of mealworms (Tenebrio molitor). Online J Biol Sci 18(1):24-28. doi:10.3844/ojbsci.2018.24.28
  10. Peng B.Y., Y. Li, R. Fan, Z. Chen, J. Chen, A.M. Brandon, C.S. Criddle, Y. Zhang, and W.M. Wu 2020, Biodegradation of low-density polyethylene and polystyrene in superworms, larvae of Zophobas atratus (Coleoptera: Tenebrionidae): Broad and limited extent depolymerization. Environ Pollut 266:115206. doi:10.1016/j.envpol.2020.115206
  11. R Core Team 2023, R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria. https://www.R-project.org/ Accessed 1 June 2024.
  12. Therneau T 2023, A package for survival analysis in R. R package version 3.5-5, https://CRAN.R-project.org/package=survival Accessed 1 June 2024.
  13. Therneau T.M. and P.M. Grambsch 2000, Modeling survival data: Extending the Cox model. Springer New York. doi:10.1007/978-1-4757-3294-8
  14. Wang X., and T. Tang 2022, Effects of polystyrene diet on the growth and development of Tenebrio molitor. Toxics 10(10):608. doi:10.3390/toxics10100608
  15. Yang S.S., A.M. Brandon, J.C. Andrew Flanagan, J. Yang, D. Ning, S.Y. Cai, H.Q. Fan, Z.Y. Wang, J. Ren, E. Benbow, N.Q. Ren, R.M. Waymouth, J. Zhou, C.S. Criddle, and W.M. Wu 2018, Biodegradation of polystyrene wastes in yellow mealworms (larvae of Tenebrio molitor Linnaeus): factors affecting biodegradation rates and the ability of polystyrene-fed larvae to complete their life cycle. Chemosphere 191:979-989. doi:10.1016/j.chemosphere.2017.10.117
  16. Yang S.S., M.Q. Ding, X.R. Ren, Z.R. Zhang, M.X. Li, L.L. Zhang, J.W. Pang, C.X. Chen, L. Zhao, D.F. Xing, N.Q. Ren, J. Ding, and W.M. Wu 2022, Impacts of physical-chemical property of polyethylene on depolymerization and biodegradation in yellow and dark mealworms with high purity microplastics. Sci Total Environ 828:154458. doi:10.1016/j.scitotenv.2022.154458
  17. Yang Y., J. Yang, W.M. Wu, J. Zhao, Y. Song, L. Gao, R. Yang, and L. Jiang 2015, Biodegradation and mineralization of polystyrene by plastic-eating mealworms: Part 1. Chemical and physical characterization and isotopic tests. Environ Sci Technol 49(20):12080-12086. doi:10.1021/acs.est.5b02661
  18. Zhong Z., X. Zhou, Y. Xie, and L.M. Chu 2023, The interplay of larval age and particle size regulates micro-polystyrene biodegradation and development of Tenebrio molitor L. Sci Total Environ 857:159335. doi:10.1016/j.scitotenv.2022.159335
  19. Zielinska E., D. Zielinski, A. Jakubczyk, M. Karas, U. Pankiewicz, B. Flasz, M. Dziewiecka, and S. Lewicki 2021, The impact of polystyrene consumption by edible insects Tenebrio molitor and Zophobas morio on their nutritional value, cytotoxicity, and oxidative stress parameters. Food Chem 345:128846. doi:10.1016/j.foodchem.2020.128846