Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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A Case of Canine Mammary Comedocarcinoma with Regulatory T Cell Infiltration

  • Siwon Jeong (College of Veterinary Medicine, Jeju National University) ;
  • Jiwoong Yoon (College of Veterinary Medicine, Jeju National University) ;
  • Woo-Jin Song (Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Jeju National University) ;
  • Jongtae Cheong (College of Veterinary Medicine, Jeju National University) ;
  • Young-min Yun (Research Institute of Veterinary Medicine, College of Veterinary Medicine, Jeju National University) ;
  • Gee Euhn Choi (Research Institute of Veterinary Medicine, College of Veterinary Medicine, Jeju National University) ;
  • Myung-Chul Kim (Research Institute of Veterinary Medicine, College of Veterinary Medicine, Jeju National University)
  • Received : 2024.06.13
  • Accepted : 2024.08.02
  • Published : 2024.08.31
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Abstract

An adult female dog was presented for evaluation of rapid growth of mammary gland masses. Complete blood count, serum biochemistry, and diagnostic imaging results were unremarkable. Fine needle aspirates of the mammary masses indicated mammary carcinoma characterized by large globoid cells with finely granular eosinophilic globules or Melamed-Wolinska-like bodies. A regional mastectomy was performed on the masses. Subsequent histopathologic examination of the surgically resected masses resulted in a diagnosis of mammary comedocarcinoma with nodal metastasis and distinct perivascular immune infiltrates, which were subject to immunohistochemical and flow cytometric immunophenotyping. Immunohistochemical examination confirmed the infiltration of CD3+ T and PAX5+ B lymphocytes. Flow cytometric analysis demonstrated tumor-infiltrating CD4+CD25+FOXP3+ regulatory T, CD8+ T, CD11b+ myeloid, and CD21+ B cells. Of note, paired flow cytometric analysis of peripheral blood and tumor tissues showed a preferential tumor infiltration of regulatory T and B cells. Approximately two months after the mastectomy, the tumor reoccurred at the surgery site. The dog died due to deteriorating conditions. We report a rare case of canine mammary comedocarcinoma, providing clinical, clinicopathologic, histologic, and immunophenotypic characteristics. Our case is valuable in providing a rationale for basic research that maps the immune landscape of mammary comedocarcinoma to identify key immune subsets for cancer progression.

Keywords

Acknowledgement

The authors express sincere appreciation to all the pathologist, including Ha-Young Lim, from Green vet Laboratories involved with the pathology and immunohistochemistry reports used in this study. We thank Jin-Hyeon Kim (JNBIO Co., Ltd.), Jaewook Shin (BMIKOREA Co., Ltd.), and Ju-Hun Kim (BioApplications Inc.) for their academic and technical consultation on this study. This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (MOE) (#RS-2023-00241779). This research was also supported by Regional Innovation Strategy (RIS) through the NRF funded by MOE (#2023RIS-009).

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