Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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A Case of Canine Colorectal Carcinoma In Situ with Regulatory T Cell Infiltration

  • Yunhee Joung (Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Jeju National University) ;
  • Jiwoong Yoon (College of Veterinary Medicine, Jeju National University) ;
  • Dong Ju Lee (College of Veterinary Medicine, Jeju National University) ;
  • Woo-Jin Song (Laboratory of Veterinary Internal Medicine, College of Veterinary Medicine, Jeju National University) ;
  • Jongtae Cheong (Department of Veterinary Medicine, College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University) ;
  • Hyunjung Park (Department of Veterinary Medicine, College of Veterinary Medicine and Veterinary Medical Research Institute, Jeju National University) ;
  • Young-min Yun (Department of Veterinary Internal Medicine, Wildlife Rescue Center, College of Veterinary Medicine, Jeju National University) ;
  • Gee Euhn Choi (Research Institute of Veterinary Medicine, College of Veterinary Medicine, Jeju National University) ;
  • Myung-Chul Kim (Research Institute of Veterinary Medicine, College of Veterinary Medicine, Jeju National University)
  • Received : 2024.05.27
  • Accepted : 2024.07.16
  • Published : 2024.08.31
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Abstract

An adult castrated male dog was presented with persistent hematochezia. Digital rectal examination and endoscopy found multiple colorectal masses. Complete blood count and serum biochemical results were within the reference interval. Fine needle aspirate of the masses indicated a diagnosis of inflamed polyps with a primary differential of malignancy. Histopathologic examination using endoscopy-guided incisional biopsy of the masses revealed an inflamed neoplasm with ossification. A colectomy was performed to remove the tumor. Subsequent histopathologic examination of the surgically resected masses resulted in a diagnosis of colorectal carcinoma in situ (CiS) with immune infiltrates, which were subject to immunohistochemical and flow cytometric immunophenotyping. The immunohistochemistry confirmed intraepithelial CD3+ T cells within CiS. The flow cytometric analysis indicated tumor-infiltrating CD4+ T, CD8+ T, and CD11b+ myeloid subsets. The flow cytometric analysis of circulating and tumor-infiltrating leukocytes demonstrated a preferential expansion of CD25+FOXP3+ regulatory T cells within CiS. To the author's knowledge, this is the first report to show clinical evidence emphasizing the immunogenicity and immune-suppressive environment of canine colorectal CiS. Our case will be valuable in providing a rationale for basic research that dissects the immune environment for canine colorectal cancers for the future development of cancer immunotherapy.

Keywords

Acknowledgement

The authors express sincere appreciation to all the pathologists from IDEXX Laboratories involved with the pathology and immunohistochemistry reports used in this study. This research was supported by the Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (MOE) (#RS-2023-00241779).

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