대한한의학방제학회지 (Herbal Formula Science)

  • 제32권3호
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  • Pages.203-221
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  • 2024
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  • 1229-1218(pISSN)
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  • 2288-5641(eISSN)
대한한의학방제학회 (The Korean Medicine Society for the Herbal Formula Study)
권호 표지
DOI QR코드

DOI QR Code

산화적스트레스에 대한 향산온담탕(香山溫膽湯)의 간보호(肝保護) 효과

Hyangsanondam-tang protects oxidative stress induced-liver damage

  • 박상미 (대구한의대학교 한의과대학) ;
  • 정대화 (대구한의대학교 제약공학과) ;
  • 변성희 (대구한의대학교 한의과대학) ;
  • 김상찬 (대구한의대학교 한의과대학)
  • Sang Mi Park (College of Korean Medicine, Daegu Haany University) ;
  • Dae Hwa Jung (Department of Pharmaceutical Engineering, Daegu Haany University) ;
  • Sung Hui Byun (College of Korean Medicine, Daegu Haany University) ;
  • Sang Chan Kim (College of Korean Medicine, Daegu Haany University)
  • 투고 : 2024.07.08
  • 심사 : 2024.08.11
  • 발행 : 2024.08.31
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초록

Objective: Hyangsanondam-tang (⾹⼭溫膽湯, HSODT) is one of the Korean herbal formula for treatment of insomnia, neurosis and anxiety induced by liver Qi stagnation and unhealthy heart. We investigated the hepatoprotective effect of HSODT against arachidonic acid (AA) + iron-mediated cytotoxicity in HepG2 cells. Methods: AA + iron induced oxidative stress and mitochondrial dysfunction in HepG2 cells. The cytoprotective effects of HSODT were determined by MTT assay, western blot and fluorescence activated cell sorting analysis. Results: In HepG2 cells, pretreatment with HSODT significantly suppressed cytotoxicity and the expression of proteins related to apoptosis induced by AA + iron. In addition, pretreatment with HSODT significantly prevented the increase of H2O2 production, and decrease GSH depletion and mitochondrial membrane potential induced by AA + iron. Conclusions : These results indicated that HSODT could protect against oxidative stress-induced liver damage.

키워드

과제정보

This research was supported by a grant from Daegu Haany University Ky.lin Foundation in 2023.

참고문헌

  1. Kim SC. Establishment of Hyangsanondamtang through the analysis of Hyangsan's clinical chart. The 3rd Joint Conference of Society of Herbal Medicine & Society of Oriental Medicinal Formula. 2023. 79-96.
  2. Kang SB, Kim YJ, Woo CH. The Effect of Gamiondam-tang on Sleep Disorder after Traffic Accident: a Clinical Case Report. The Journal of East-West Medicine. 2020;45(2):13-20.
  3. Kong WH, Kim WI. A Case Report on 14 Patients with Palpitations Treated with Gami-ondam-tang. J. Int. Korean Med. 2021;42(5):1160-72. https://doi.org/10.22246/jikm.2021.42.5.1160
  4. Jeong HJ, Jeon SY. A Case Report of the Treatment of an Unstable Angina Pectoris Patient with Chest Pain, Insomnia, and Dyspnea by Gamiondam-tang-gagam. J. Int. Korean Med. 2019;40(3):525-33. https://doi.org/10.22246/jikm.2019.40.3.525
  5. Kang TL. Effect of Gami-Ondamtang(GO) on brain neuronal transmitters in immobilized rats. Kor. J. Oriental Preventive Medical Society. 2001;5(2):114-21.
  6. Kim KS, Jeun SY, Ann JJ, Hong S, Jeung SM. Study on Antihyperlipidemic Effect of Kamiondamtang. Th Korean Journal of Oriental Medical Prescription. 2005;13(1):85-101.
  7. Kim HC, Jeong DG. A study of the comparative effect of Kuibitang, Kamiondarmtang, and Kuibiondarmtang on serum levels in rats under the immobilization stress. J. of Oriental Neuropsychiatry. 1993;4(1):99-119.
  8. Eom SH, Hong MC, Shin MK, Kim WH. Experimental study on the effect of Gamiondarmtang on sleep time. The Journal of East-West Medicine. 1983;8(2):1-11
  9. Jin HJ, Park SM, Kim EO, Kim SC. Hepatoprotective effect of Ikwiseungyang-tang via Nrf2 activation. Herb. Formula Sci. 2021;29(4):167-179.
  10. Dong GZ, Lee JH, Ki SH, Yang JH, Cho IJ, Kang SH, Zhao RJ, Kim SC, Kim YW. AMPK activation by isorhamnetin protects hepatocytes against oxidative stress and mitochondrial dysfunction. Eur J Pharmacol. 2014:740:634-40. https://doi.org/10.1016/j.ejphar.2014.06.017
  11. Yin H, Zhou Y, Zhu M, Hou S, Li Z, Zhong H, Lu J, Meng T, Wang J, Xia L, Xu Y, Wu Y. Role of mitochondria in programmed cell death mediated by arachidonic acid-derived eicosanoids. Mitochondrion. 2013;13(3):209-24. https://doi.org/10.1016/j.mito.2012.10.003
  12. Li B, Nasser M, Masood M, Adlat S, Huang Y, Yang B, Luo C, Jiang N. Efficiency of Traditional Chinese medicine targeting the Nrf2/HO-1 signaling pathway. Biomed Pharmacother. 2020;126:110074.
  13. Taguchi K, Motohashi H, Yamamoto M. Molecular mechanisms of the Keap1-Nrf2 pathway in stress response and cancer evolution. Genes Cells. 2011;16(2):123-40. https://doi.org/10.1111/j.1365-2443.2010.01473.x
  14. Tejo FV, Quintanilla RA. Contribution of the Nrf2 Pathway on Oxidative Damage and Mitochondrial Failure in Parkinson and Alzheimer's Disease. Antioxidants 2021, 10, 1069
  15. Hyangsan Liberal Arts Education Research Institute of Daegu Haany University. The scholarship and era positioning of Dr. Byun JH. 2022.
  16. http://weekly.khan.co.kr/khnm.html?mode=view&art_id=17534
  17. https://www.isom.or.kr/bbs/content.php?co_id=c1_menu01
  18. https://www.akom.org/Home/Company/2?sub=10
  19. Lee DG. Hyangsan's life about 'Contributing to the country through education'. Hyangsan Liberal Arts Education Research Institute of Daegu Haany University. 2022;23-38.
  20. Byun JH. Edited by Gyeongsan University College of Oriental Medicine Alumni Association. Dr. Byun JH's special clinical lecture notes. Gyeongsan University College of Oriental Medicine Alumni Association. 1996.
  21. Hwang DY. Bangyakhappyeon. Seoul:Namsandang. 1985:205.
  22. Heo J. Donguibogam. Seoul:Namsandang. 1987:96.
  23. Wang T, Fu X, Chen Q, Patra JK, Wang D, Wang Z, Gai Z. Arachidonic Acid Metabolism and Kidney Inflammation. Int. J. Mol. Sci. 2019;20:3683.
  24. Li Y, Zhao H, Wang Y, Zheng H, Yu W, Chai H, Zhang J, John R. Falck, Guo AM, Yue J, Peng R, Yang J. Isoliquiritigenin induces growth inhibition and apoptosis through downregulating arachidonic acid metabolic network and the deactivation of PI3K/Akt in human breast cancer. Toxicol Appl Pharmacol. 2013;;272(1):37-48. https://doi.org/10.1016/j.taap.2013.05.031
  25. Shin SM, Kim SG. Inhibition of arachidonic acid and iron-induced mitochondrial dysfunction and apoptosis by oltipraz and novel 1,2-dithiole-3-thione congeners. Mol Pharmacol. 2009;75(1):242-53. https://doi.org/10.1124/mol.108.051128
  26. Lee EH, Baek SY, Kim KY, Lee SG, Kim SC, Lee HS, Kim YW. Effect of Rheum undulatum Linne extract and Glycyrriza uralensis Fischer extract against arachidonic acid and iron-induced oxidative stress in HepG2 cell and CCl4-induced liver injury in mice. Herb. Formula Sci. 2016;24(3):163-74. https://doi.org/10.14374/HFS.2016.24.3.163
  27. Zhang X, Yu Y, Lei H, Cai Y, Shen J, Zhu P, He Q, Zhao M. The Nrf-2/HO-1 Signaling Axis: A Ray of Hope in Cardiovascular Diseases. Cardiol Res Pract. 2020;2020:5695723.
  28. Ulasov AV, Rosenkranz AA, Georgiev GP, Sobolev AS. Nrf2/Keap1/ARE signaling: Towards specific regulation. Life Sci. 2022;291:120111.
  29. Lee JS, Surh YJ. Nrf 2 as a novel molecular target for chemoprevention. Cancer Letters. 2005;224:171-84. https://doi.org/10.1016/j.canlet.2004.09.042
  30. Zhang Q, Liu J, Duan H, Li R, Peng W, Wu C. Activation of Nrf2/HO-1 signaling: An important molecular mechanism of herbal medicine in the treatment of atherosclerosis via the protection of vascular endothelial cells from oxidative stress. J Adv Res. 2021;34:43-63. https://doi.org/10.1016/j.jare.2021.06.023
  31. Yang JH, Choi MH, Na CS, Cho SS, Kim JH, Ku SK, Cho IJ, Shin HJ, Ki SH. Bamboo Stems (Phyllostachys nigra variety henosis) Containing Polyphenol Mixtures Activate Nrf2 and Attenuate Phenylhydrazine-Induced Oxidative Stress and Liver Injury. Nutrients. 2019;;11(1):114.
  32. Cho NJ, Lee WH, Kim KK, Han HS. Investigation of the Antioxidant Effect of Angelicae Radix from Korea, China and Japan. J Physiol & Pathol Korean Med. 2017;31(3):182-7. https://doi.org/10.15188/kjopp.2017.06.31.3.182
  33. Hwang EY, Kim1 DH, Kim HJ, Hwang JY, Park TS, Lee IS, Son JH. Antioxidant Activities and Nitric Oxide Production of Medicine Plants in Gyeongsangbukdo (Carthamus tinctorius seed, Cyperus rotundus, Schizonepeta tenuifolia, Polygonatum odoratum var. pluriflorum, Paeonia Lactiflora). J. Appl. Biol. Chem. 2011;54(3):171-7. https://doi.org/10.3839/jabc.2011.029
  34. Joo JC, Shin JH, Lee SJ, Cho HS, Sung NJ. Antioxidative Activity of Hot Water Extracts from Medicinal Plants. J Korean Soc Food Sci Nutr. 2006;35(1):7-14. https://doi.org/10.3746/jkfn.2006.35.1.007
  35. Oh YL. Effect of Antioxidant of Citri Reticulatae Pericarpium on Cytotoxicity of Oxygen Free Radicals in Cultured NIH3T3 Fibroblast. Kor. J. Herbology. 2006;21(4):143-8.
  36. Liu S, Li M, Liu W, Zhang Z, Wang X, Dong H. Structure and properties of acidic polysaccharides isolated from Massa Medicata Fermentata: Neuroprotective and antioxidant activity. Int J Biol Macromol. 2024;259:129128.
  37. Yang HJ, Park MJ, Lee HS. Antioxidative Activities and Components of Gardenia jasminoides. Korean J. Food Sci. Technol. 2011;43(1):51-7. https://doi.org/10.9721/KJFST.2011.43.1.051
  38. Tang D, Yan R, Sun Y, Kai G, Chen K, Li J. Material basis, effect, and mechanism of ethanol extract of Pinellia ternata tubers on oxidative stress-induced cell senescence. Phytomedicine. 2020;77:153275.
  39. Kong GB, Kwon OH. Antioxidant Effects of Ponciri Fructus Bioconversion Extracts as Cosmetic Ingredients. Asian J Beauty Cosmetol. 2021;(19)3:477-85. https://doi.org/10.20402/ajbc.2021.0205
  40. Park YM, Kim SJ, Jo KH, Yang EJ, Jung SH. Anticariogenic and Antioxidant Activities from Medicinal Herbs. J Korean Soc Food Sci Nutri. 2006;35(3):284-93. https://doi.org/10.3746/jkfn.2006.35.3.284
  41. Lee SE, LEE SW, Bang JK, Yu YJ, Seong NS. Antioxidant Activities of Leaf, Stem and Root of Panax ginseng C. A. Meyer. Korean J. Medicinal Crop Sci. 2004;12(2):237-42.
  42. Kim JY, Lee HY, Jo WS, Park SC. Comparison of composition and antioxidant activity of Poria cocos Wolf cultivated in a mortuary and cemetery. J. Mushrooms. 2018;16(2):111-7. https://doi.org/10.14480/JM.2018.16.2.111
  43. Jang GY, Kim HY, Lee SH, Kang YR, Hwang IG, Woo KS, Kang TS, Lee JS, Jeong HS. Effects of Heat Treatment and Extraction Method on Antioxidant Activity of Several Medicinal Plant. J Korean Soc Food Sci Nutr. 2012;41(7):914-20. https://doi.org/10.3746/jkfn.2012.41.7.914
  44. Jang JS. Antioxidant Activities of Liriope platyphylla L. Extracts Obtained from Different Solvents. Korean J. Food Nutr. 2018;31(4):543-8.
  45. Choi CY, Lee KJ, Jeong HG. Effects of Aqueous Extract Isolated from Platycodon grandiflorum Against t-Buty lhydroperoxide-induced Oxidative Stress in Rat Primary Hepatocytes. Yakhak Hoeji. 2002;46(4):466-71.
  46. Choi SY, Kim KT. Anti-oxidative and muscle cell protective effects of ginger extract according to the extraction conditions. Korean J Food Preserv. 2002;29(5):762-7. https://doi.org/10.11002/kjfp.2022.29.5.762
  47. Hong JY, Nam HS, Shin SR. Changes on the Antioxidant Activities of Extracts from the Ziziphus jujube Miller Fruits During Maturation. Korean J. Food Preserv. 2010;17(5):712-9.