Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Complete Genome Sequence of Bacillus safensis DMB13 Exhibiting Non-Antibacterial Activity

  • Do-Won Jeong (Department of Food and Nutrition, Dongduk Women's University)
  • Received : 2024.04.23
  • Accepted : 2024.05.08
  • Published : 2024.06.28
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Abstract

Strain Bacillus safensis DMB13 exhibiting protease and lipase activity was isolated from fermented kimchi in the previous study. Phenotypically, strain DMB13 showed no antibacterial activity. Thus, the complete genome sequence was analyzed to understand the phenotype of strain DMB13. The genome is 3,856,276-bp with a G+C content of 41.61 mol% and consists of a single circular 3,849,633-bp chromosome and one circular plasmids. Two genes related to bacteriocin production, skfF and skfG, were identified; however, six other genes in the skf operon were not detected. The genome includes 54 protease- and 5 lipase-encoding genes.

Keywords

Acknowledgement

This study was conducted in support of the sabbatical program of Dongduk Women's University in 2023.

References

  1. Satomi M, La Duc MT, Venkateswaran K. 2006. Bacillus safensis sp. nov., isolated from spacecraft and assembly-facility surfaces. Int. J. Syst. Evol. Microbiol. 56: 1735-1740. https://doi.org/10.1099/ijs.0.64189-0
  2. Romero-Severson J, Moran TE, Shrader DG, Fields FR, Pandey-Joshi S, Thomas CL, et al. 2021. A seed-endophytic Bacillus safensis strain with antimicrobial activity has genes for novel bacteriocin-like antimicrobial peptides. Front. Microbiol. 12: 734216.
  3. Wu PS, Liu CH, Hu SY. 2021. Probiotic Bacillus safensis NPUST1 administration improves growth performance, gut microbiota, and innate immunity against Streptococcus iniae in Nile tilapia (Oreochromis niloticus). Microorganisms 9: 2494.
  4. Kim T, Heo S, Na HE, Lee G, Kim JH, Kwak MS, et al. 2022. Bacterial community of galchi-baechu kimchi based on culture-dependent and - independent investigation and selection of starter candidates. J. Microbiol. Biotechnol. 32: 341-347. https://doi.org/10.4014/jmb.2112.12003
  5. Tatusov RL, Galperin MY, Natale DA, Koonin EV. 2000. The COG database: a tool for genome-scale analysis of protein functions and evolution. Nucleic Acids Res. 28: 33-36. https://doi.org/10.1093/nar/28.1.33
  6. Aziz RK, Bartels D, Best AA, DeJongh M, Disz T, Edwards RA, et al. 2008. The RAST Server: rapid annotations using subsystems technology. BMC Genomics 9: 75.
  7. Kwon G-H, Lee, HA, Kim, J-H. 2010. A bacteriocin of 5-kDa in size secreted by Bacillus subtilis 168. Korean J. Microbiol. Biotechnol. 38: 163-167.
  8. Allenby NE, Watts CA, Homuth G, Pragai Z, Wipat A, Ward AC, et al. 2006. Phosphate starvation induces the sporulation killing factor of Bacillus subtilis. J. Bacteriol. 188: 5299-5303. https://doi.org/10.1128/JB.00084-06
  9. Jeong DW, Heo S, Ryu S, Blom J, Lee JH. 2017. Genomic insights into the virulence and salt tolerance of Staphylococcus equorum. Sci. Rep. 7: 5383.