Korean Journal of Agricultural Science (농업과학연구)

Institute of Agricultural Science, CNU (충남대학교 농업과학연구소)
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Residual characteristics and safety assessments of prochloraz and its metabolites in Aster yomena using QuEChERS and LC-MS/MS

  • Hyeon-Jin Lim (Agricultural Chemistry, Chungnam National University) ;
  • Young-Shin Kim (Agricultural Chemistry, Chungnam National University) ;
  • Chi-Hwan Lim (Agricultural Chemistry, Chungnam National University)
  • Received : 2024.03.20
  • Accepted : 2024.05.16
  • Published : 2024.06.01
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Abstract

Although Aster yomena has recently attracted attention for its potential anti-cancer, antimicrobial, and immune-boosting effects, there are concerns about residual pesticides because they are consumed in salad-like forms. This study investigated residual characteristics and human dietary risks of the imidazole fungicide prochloraz applied to the herbal medicine Aster yomena with different spray frequency and timing. Residual analysis of prochloraz and its three main metabolites (BTS44595, BTS44596, and 2,4,6-trichlorophenol) in Aster yomena samples was performed using the QuEChERS method and LC-MS/MS. Mean recovery rates of the fungicide and its metabolites were satisfactory in the range of 80.1 ± 1.2% to 108.2 ± 3.8%. The residual concentration of the fungicide calculated as the sum of prochloraz and its metabolites was the highest (4.14 mg·kg-1) in the Aster yomena sample applied three times at weekly intervals with the fungicide until immediately before harvest. The fungicide residue concentration in the Aster yomena was below the method-limit of quantification (MLOQ) when it was applied twice at the interval of 9 day until 21 days before harvest. The theoretical maximum daily intake of prochloraz, calculated based on the daily intake of Aster yomena, mean adult body weight, and the highest residue level analyzed in the this study, was safe at < 80% of the acceptable daily intake of the fungicide (0.01 mg-1·kg·bw-1·day). In conclusion, the triple application method with prochloraz at weekly intervals until the harvest day is recommended to produce safe Aster yomena from the fungicide residues and risks to humans.

Keywords

Acknowledgement

본 연구는 농촌진흥청(Rural Development Administration)의 농약 직권 등록 작물 잔류 시험(2023-AR-0538)으로 수행되었습니다.

References

  1. Bae BJ, Lee HK, Son KA, Im GJ, Kim JB, Kim TH, Chae S, Park JW. 2012. The residue property of fungicide boscalid and fluidioxonil at the same time harvest leafy-vegetables. The Korean Journal of Pesticide Science 16:98-108. [in Korean]
  2. CAC (Codex Alimentarius Commission). 2010. Guidelines on good laboratory practice in pesticide residue analysis. CAC, Rome, Italy.
  3. Choi YH, Han SS, Kim IK. 2002. Determination of residual concentration and half-life time in soils of imidazole fungicide prochloraz. Analytical Science & Technology 15:67-71. [in Korean]
  4. EPA (U.S. Environmental Protection Agency), IRIS (Integrated Risk Information System). 1989. Chemical Assessment Summary, prochloraz. EPA, Washington, D.C., USA.
  5. EURL-SRM (EU Reference Laboratorie for Residues of Pesticides-Single Residue Methods). 2014. Determination of prochloraz (sum) via its metabolites version 1, Europe. European Commission, Fellbach, Germany.
  6. Fang Q, Yao G, Shi Y, Ding C, Wang Y, Wu X, Hua R, Cao H. 2017. Residue dynamics and risk assessment of prochloraz and its metabolite 2,4,6-trichlorophenol in apple. Molecules 22:1780-1788.
  7. Gu SY, Lee SJ, Lee SE, Park CY, Lee JM, Park IJ, Chung YM, Jang GH, Moon GL. 2023. Selection and validation of an analytical method for trifludimoxazin in agricultural products with LC-MS/MS. Journal of Food Hygiene and Safety 38:79-88. [in Korean]
  8. Hwang KA. 2020. Functional food for immune regulation focusing on Korean native materials. Food Industry and Nutrition 25:11-18. [in Korean]
  9. Jin MJ, Park HK, Jeong HR, Lee JW, Jo SH, Noh HH, Lee JY, Kim JS, Kwon CH, Kim JE, et al. 2018. Residual characteristics and safety assessments of the fungicide fenhexamid in some minor crops. The Korean Journal of Pesticide Science 22:363-369. [in Korean]
  10. KCPA (Korea Crop Protection Association). 2013. Crop protection products Q&A. Accessed in https://new.koreacpa.org/crop-protection/qa/166/ on 5 January 2024. [in Korean]
  11. KHIDI (Korea Health Industry Development Institute). 2021. Food intake statistics. Accessed in https://www.khidi.or.kr/kps/dhraStat/intro?menuId=MENU01650&year=2021#sub04 on 5 January 2024. [in Korean]
  12. Kim YJ, Song JW, Chio SG, Kim JH. 2021. Residual characteristics of fungicides azoxystrobin, fluxapyroxad, and penthiopyrad on Peucedanum japonicum Thunb. The Korean Journal of Pesticide Science 25:415-424. [in Korean]
  13. Kim YS, Yang JY, Jin NY, Yu YM, Youn YN, Lim CH. 2017. Residue analysis of spinetoram and spinosad on paprika leaf using the modified QuEChERS pre-treatment methods. Korean Journal of Agricultural Science 44:487-494. [in Korean]
  14. Lee EY. 2018. When PLS is implemented, 931 farms receive inappropriate judgments for their agricultural products. Accessed in http://www.farminsight.net/news/articleView.html?idxno=878 on 5 January 2024. [In Korean]
  15. Lee HJ. 2014. The benefits of angelica, good for cough and asthma but poisonous for diarrhea Accessed in https://m.health.chosun.com/svc/news_view.html?contid=2014102702152 on 5 January 2024.
  16. Lee JA. 2015. Quality characteristics of cookies added with Aster yomena Powder. The Korean Journal of Culinary Research 21:141-153. [in Korean]
  17. MFDS (Ministry of Food and Drug Safety). 2017a. Notice of PLS introduction. Accessed in https://mfds.go.kr/brd/m_74/view.do?seq=36124&srchFr=&srchTo=&srchWord=&srchTp=&itm_seq_1=0&itm_seq_2=0&multi_itm_seq=0&company_cd=&company_nm=&page=69 on 5 January 2024. [In Korean]
  18. MFDS (Ministry of Food and Drug Safety). 2017b. International harmonization of supervised trials for evaluation of the pesticide residue. MFDS, Cheongju, Korea. [in Korean]
  19. MFDS (Ministry of Food and Drug Safety). 2023a. Pesticide MRLs in food. MFDS, Cheongju, Korea. [in Korean]
  20. MFDS (Ministry of Food and Drug Safety). 2023b. Practical guide for food code residue pesticide testing methods. MFDS, Cheongju, Korea. [in Korean]
  21. MFDS (Ministry of Food and Drug Safety). 2023c. Korean food composition database. Accessed in https://data.mfds.go.kr/nsd/obaaa/stdDbSrchRsltList.do on 5 January 2024. [in Korean]
  22. NAQS (National Agricultural Products Quality Management Service). 2022. Agricultural products residual pesticide analysis method. NAQS, Jeonju, Korea. [in Korean]
  23. NAQS (National Agricultural Products Quality Management Service). 2023. Registration criteria for pesticides and technical materials. NAQS, Jeonju, Korea. [in Korean]
  24. Oh KY, Lee DY, Song TB, Kim YJ, Kim JY. 2022. Residual dissipation pattern and the safety assessment of tebufenozide and teflubenzuron on Black Chokeberry (Aronia melanocarpa). The Korean Journal of Pesticide Science 26:95-102. [in Korean]
  25. Park BJ, Choi JH, Kim CS, Lee BM, Ihm YB, Cho IK. 2003. Safe and easy disposal of prochloraz wastewaters after used as rice seed disinfectant. The Korean Journal of Pesticide Science 7:169-175. [in Korean]
  26. Park JS, Choi H. 2020. Simultaneous determination for fungicide prochloraz and its metabolites in animal commodities with GC-ECD after hydrolysis. Journal of Applied Biological Chemistry 63:153-159. [in Korean]
  27. Park JU, Bae BJ, Woo SW, Jeong HJ, Jang YJ, Park JW, Chai S, Lee KS, Keum YS, Kyung KS, et al. 2022. Residual characteristics and risk assessments of afidopyropen, pydiflumetofen and mefentrifluconazole in perilla leaves. The Korean Journal of Pesticide Science 26:65-73. [in Korean]
  28. RDA (Rural Development Administration). 2020. Pesticide evaluation procedure. Accessed in https://psis.rda.go.kr/psis/cont/contentMain.ps?menuId=PS00308 on 5 January 2024. [in Korean]
  29. Seo SW. 2019. Protective effects of ethanol extract from Aster Yomena on acute pancreatitis. Journal of Physiology & Pathology in Korean Medicine 33:109-115. [in Korean]
  30. Shimshoni JA, Bommuraj V, Chen Y, Sperling R, Barel S, Feygenberg O, Maurer D, Alkan N. 2020. Posthavest fungicide for avocado fruits: Antifungal efficacy and peel to pulp distribution kinetics. Foods 9:124-137.
  31. Tian F, Qiao C, Wang C, Pang T, Guo L, Li J, Pang R, Xie H. 2022. Dissipation behavior of prochloraz and its metabolites in grape under open-field, storage and the wine-making process. Journal of Food Composition and Analysis 114:104846.
  32. You YH, Lee YS, Kwon HJ. 2011. Reduction factors of pesticides with different physicochemical properties under washing and cooking conditions. Korean Journal of Food Science and Technology 43:537-543.
  33. Zhao HY, Yang GI, Liu YI, Ye HP, Qi XJ, Wang Q. 2019. Residual behavior and risk assessment of prochloraz in bayberries and bayberry wine for the Chinese population. Environmental Monitoring and Assessment 191:644.