산업진흥연구 (Industry Promotion Research)

  • 제9권3호
  • /
  • Pages.77-85
  • /
  • 2024
  • /
  • 2466-1139(pISSN)
  • /
  • 2714-013X(eISSN)
산업진흥원 (Industrial Promotion Institute)
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감국꽃 열수 추출물의 생리활성에 관한 연구

Study on the Physiological Activity of Chrysanthemum indicum Flower Extracts Using the Hydrothermal Water Extraction Method

  • 하지성 (한남대학교 코스메틱사이언스학과) ;
  • 강유리 (한남대학교 화학과) ;
  • 김운중 (한남대학교 화학과)
  • Jisung Ha (Department of Cosmetic Science, Hannam University) ;
  • Yuri Kang (Department of Chemistry, Hannam University) ;
  • Woonjung Kim (Department of Chemistry, Hannam University)
  • 투고 : 2024.06.23
  • 심사 : 2024.08.11
  • 발행 : 2024.07.31
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초록

본 연구에서는 감국꽃 열수 추출물의 분석을 통하여 기능성 소재 가치를 확인하고자 하였다. GC-MS를 통한 성분분석 결과 다양한 방향족 화합물이 검출되었으며, 항산화 분석을 통하여 추출물의 높은 항산화 효능을 확인하였다. 가장 높은 활성이 나타난 에탄올 함량 70% 추출물을 대상으로 TIME KILL TEST를 이용한 시험에서 높은 향균력을 확인하였고, 세포 내 멜라닌 생성 저해 시험 결과 추출물을 1%, 1.2%, 1.5%의 농도로 처리하였을 때 각각의 농도에서 α-MSH 처리군 대비 115.77 ± 6.67%, 112.42 ± 1.64%, 67.12 ± 3.89%로 확인되었다. Collagenage 활성 저해 분석 결과 1.5%, 3%, 5%의 농도로 처리하였을 때 56.57 ± 2.28%, 58.64 ± 2.99, 29.53 ± 1.03%의 Collagenase 억제율을 확인하였다. 추출물에 대한 세포독성 시험 결과 추출물의 농도가 1.5%, 3%, 5%일 때 세포 독성이 나타나지 않는 것으로 확인하였다. 따라서 본 연구를 통하여 감국꽃의 열수 추출물이 기능성 화장품 소재 가치로 유용할 것으로 예측한다.

In this study, the hydrothermal extract of Chrysanthemum indicum Flowers are analyzed for the identification of its functional material value. Various aromatic compounds were detected as a result of GC-MS analysis, and antioxidant analysis confirmed the high antioxidant activity of the extract. High antibacterial activity was confirmed in the TIME KILL TEST using the 70% ethanol extract, which showed the highest activity, and the inhibition of intracellular melanogenesis was confirmed to be 115.77 ± 6.67%, 112.42 ± 1.64%, and 67.12 ± 3.89% compared to the α-MSH treatment group at concentrations of 1%, 1.2%, and 1.5%, respectively, when the extract was treated with 1%, 1.2%, and 1.5%. Collagenage activity inhibition assay showed collagenase inhibition of 56.57 ± 2.28%, 58.64 ± 2.99 and 29.53 ± 1.03% when treated at concentrations of 1.5%, 3% and 5%. The cytotoxicity test of the extract showed no cytotoxicity at concentrations of 1.5%, 3% and 5%. Therefore, this study predicts that the hydrothermal extract of Chrysanthemum indicum Flowers will be useful as a functional cosmetic material.

키워드

과제정보

This work was supported by the "Leaders in Industry-university Cooperation 3.0" Project and 2024 Local University Revitalization Project funded by the National Research Foundation of Korea.

참고문헌

  1. J. M. Hyun, Y . J. Jo, Y . B. Kim, S. M. Park, K. S. Y oon, ,N. H. Lee, "Anti-inflammatory and Anti-oxidative Activities of Flavonoids Extracted from Dendranthema indicum Flowers in Jeju Island", Journal of the Korean Applied Science and Technology. 36(4), pp. 1259-1267, (2019) 
  2. J. Y. Kim, H. J. Yang, K. H. Lee, S. M. Jeon, Y. J. Ahn, B. R. Won, S. N. Park, "Antioxidative and Antiaging Effects of Jeju Native Plant Extracts (II)", J. Soc. Cosmet. Scientists Korea. 33(3), pp. 165-173, (2007) 
  3. H. J. Nam, Y. B. Kim, "Aging and Skin Aging", The journal of Korean oriental medical ophthalmology & otolaryngology & dermatology. 17(1), pp. 16-33, (2004) 
  4. J. H. Chung, "Generation Mechanism and Cause of Wrinkle", Journal of the society of cosmetic scientists of Korea. 29(2), pp. 1-35, (2003) 
  5. J. Y. Kim, M. K. Lee, B. S. Hwang, G. C. Kim, I. G. Hwang, "Antioxidant Activity and Tyrosinase Inhibitory Activities of Codonopsis lanceolata Extract and Solvent Fraction", Korean J. Food Nutr. 32(6), pp. 611-619, (2019) 
  6. Y. R. Choi, H. S. Do, D. W. Jeong, J. T. Par k, Y. S. Choi, "Reaction Stability of the Recombinant Tyrosinase-CNK Originating from the Psychrophilic Marine Microorganism Candidatus Nitrosopumilus Koreensis", Clean Technol. 22(3), pp. 175-180, (2016) 
  7. Y. H. Kim, E. J. Par k, R. Yang, "The Flow Behavior of Skin Collagen", KO REA J. FO O D SCI. TECHNO L. 27(4), pp. 576-581, (1995) 
  8. J. E. Kim, J. A. Lee, H. A. Kim, J. M. Kim, Y. H. Cho, "Effect of Vitamin C, Silicon and Iron on Collagen Synthesis and Break-Down Enzyme Expression in the Human Dermal Fibroblast Cell (HS27)* Cell (HS27)* ", Korean J Nutr. 42(6), pp. 505-515, (2009) 
  9. J. M. Hyun, Y . J. Jo, Y . B. Kim, S. M. Park, K. S. Yoon, ,N. H. Lee, "Anti-inflammatory and Anti-oxidative Activities of Flavonoids Extracted from Dendranthema indicum Flowers in Jeju Island", Journal of the Korean Applied Science and Technology. 36(4), pp. 1259-1267, (2019) 
  10. S. H. Lee, I. G. Hwang, J. W. Nho, Y. D. Chang, C. H. Lee, K. S. Woo, H. S. Jeong, "Quality Characteristics and Antioxidant Activity of Chrysanthemum indicum L., Chrysanthemum boreale M. and Chrysanthemum zawadskii K. Powdered Teas", J Korean Soc Food Sci Nutr. 38(7), pp. 824-831, (2009) 
  11. K. S. Woo, J. S. Yu, I. G. Hwang, Y. R. Lee, C. H. Lee, H. S. Yoon, J. S. Lee, H. S. Jeong, "Antioxidative Activity of Volatile Compounds in Flower of Chrysanthemum indicum, C. morifolium, and C. zawadskii", J Korean Soc Food Sci Nutr. 37(6), pp. 805-809, (2008) 
  12. Marsden S. Blois, "Antioxidant determinations by the use of a stable free radecal", Nature. 181(4617), pp. 1199-1200, (1958)