Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Inhibitory Effects of Latilactobacillus curvatus BYB3 Cell-Free Extract on Human Melanoma B16F10 Cells and Tumorigenic Mice

  • Dingyun Li (Division of Animal Science, Chonnam National University) ;
  • Xing Wang (Division of Animal Science, Chonnam National University) ;
  • Dong-June Park (Korea Food Research Institute) ;
  • Dong Hun Lee (Department of Biological Sciences, Chonnam National University) ;
  • Sejong Oh (Division of Animal Science, Chonnam National University)
  • Received : 2023.09.04
  • Accepted : 2023.10.19
  • Published : 2024.03.28
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Abstract

Latilactobacillus curvatus BYB3 (BYB3) is a species of lactic acid bacteria, formerly named Lactobacillus curvatus, which is isolated from kimchi. In this study, the effect of BYB3, Lactobacillus rhamnosus GG, and Lactobacillus acidophilus GP1B strain extracts at various concentrations was examined on B16F10, a mouse melanoma cell line. Cell viability was examined via MTT assay, and the results indicated that compared to the other two probiotics, BYB3 significantly decreased the total percentages of viable cells. The effects of BYB3 on cell migration and proliferation in B16F10 cells were evaluated using wound healing mobility and proliferation assays, respectively; the results indicated that BYB3 inhibits cell migration and proliferation in a concentration-dependent manner. Using human dermal fibroblast cells to investigate BYB3 extract in vivo had no effect on skin-related cells. Nonetheless, the BYB3 extract inhibited tumor growth in a mouse model, as demonstrated by liver slices. Therefore, this suggests that using BYB3 extract to inhibit melanoma may be a novel approach.

Keywords

Acknowledgement

This work was supported by the National Research Foundation grant (NRF) grant funded by the Korea government (2021R1A4A1031220 and RS-2023-00209264).

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