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과수 화상병 전염 차단을 위한 전정 가위 소독 방법

Disinfection Methods of Pruning Scissor for Preventing Transmission of Fire Blight

  • 임연정 (강원대학교 농업생명과학대학 스마트농업융합학과) ;
  • 함현희 (농촌진흥청 국립농업과학원 농산물안전성부 작물보호과) ;
  • 이미현 (농촌진흥청 국립농업과학원 농산물안전성부 작물보호과) ;
  • 이우형 (농촌진흥청 국립농업과학원 농산물안전성부 작물보호과) ;
  • 이용환 (농촌진흥청 국립농업과학원 농산물안전성부 작물보호과)
  • Yeon-Jeong Lim (Interdisciplinary Program in Smart Agriculture, Interdisciplinary Pragrom in Agriculture, Kangwon National University) ;
  • Hyeonheui Ham (Crop Protection Division, National Institute of Agricultural Science, Rural Development Administration) ;
  • Mi-Hyun Lee (Crop Protection Division, National Institute of Agricultural Science, Rural Development Administration) ;
  • Woohyung Lee (Crop Protection Division, National Institute of Agricultural Science, Rural Development Administration) ;
  • Yong Hwan Lee (Crop Protection Division, National Institute of Agricultural Science, Rural Development Administration)
  • 투고 : 2024.04.11
  • 심사 : 2024.06.03
  • 발행 : 2024.06.30

초록

화상병은 전정 도구 등에서 장시간 생존이 가능하고 작업 도구를 통해 화상병균이 전파될 수 있다. 특히, 우리나라에서는 꽃이나 어린 과실, 도장지 등을 제거하기 위해 전정 작업이 수시로 이루어지는데 이런 농작업이 화상병 확산의 주요인으로 알려져 있다. 따라서, 본 연구에서는 전정 가위에 묻어 있는 화상병균을 완전히 멸균하기 위하여 국내에 유통되는 소독제의 소독 효과를 검증하고 이를 이용하여 효과적인 소독 방법을 구명하였다. 화상병균을 가위 날에 접종 후에 침지 소독할 경우 70% 에탄올 및 1%와 0.2% 차아염소산나트륨의 경우에는 침지 후 10초가 지나면 바로 멸균되는 반면, 30% 이산화염소는 소독 효과가 거의 보이지 않았다. 분무 처리로 소독할 경우에는 70% 에탄올은 분무 1초 후에는 바로 멸균이 되었지만 1%와 0.2% 차아염소산나트륨은 각각 10초와 60초 후에 멸균되었다. 자연적으로 화상병이 발병한 가지를 5회 및 100회 절단한 후 전정 가위 날 표면에 묻어 있는 화상병균에 대한 침지 소독 시간에 따른 제거 효과를 검증한 결과, 70% 에탄올에 30초 동안 침지 처리한 경우 완전히 세균이 멸균되지 않았지만 60초 이상에서는 세균이 멸균되었다. 이런 결과를 볼 때 현장에서 전정 작업 시 전정 가위를 70% 에탄올에 60초 이상 수시로 침지하여 소독할 것을 제안한다.

Pathogens of fire blight can survive for a long time on pruning tools, etc., and fire blight can be spread through agricultural implements. In particular, in Korea, pruning is frequently performed to remove flowers, immature fruits, and succulent shoots, and this farm work is known to be a major factor of the spread of fire blight. Therefore, in this study, in order to completely sterilize pathogens on pruning shears, we verified the disinfection effect of disinfectants distributed domestically and used them to identify an effective disinfection method. When disinfecting by immersion after inoculating Erwinia amylovora TS3128 on the scissor blade, 70% ethanol and 1% and 0.2% sodium hypochlorite sterilized immediately 10 sec after immersion, while 30% chlorine dioxide showed little disinfection effect. When disinfecting by spraying, 70% ethanol sterilized bacteria 1 sec after spraying, but 1% and 0.2% sodium hypochlorite disinfected bacteria after 10 and 60 sec, respectively. After cutting the naturally disease occurring branches five and 100 times, the bacteria were not hardly sterilized in immersion treatment in 70% ethanol for 30 sec but perfectly disinfected over 60 sec. Considering these results, pruning shears should be disinfected by frequently immersing them in 70% ethanol for at least 60 sec during pruning work in the field.

키워드

과제정보

This study was carried out with the support of Cooperative Research Programs (Project No. RS-2020-RD009337) from Rural Development Administration, Republic of Korea.

참고문헌

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