Korean Journal of Plant Resources (한국자원식물학회지)

The Plant Resources Society of Korea (한국자원식물학회)
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Effect of Alternating Temperature Conditions, and GA3 Treatment on Seed Germination of Trollius macropetalus (Regel) F.Schmidt

변온 조건 및 GA3 처리가 큰금매화의 종자 발아에 미치는 영향

  • Dong-Hak Kim (DMZ Botanic Garden, Korea National Arboretum) ;
  • Seungju Jo (Department of Forest Environmental System, Kangwon National University) ;
  • Jung-Won Sung (Department of Landscape Architecture, Korea National University of Agriculture and Fisheries) ;
  • Jung-Won Yoon (DMZ Botanic Garden, Korea National Arboretum)
  • 김동학 (국립수목원, DMZ산림생물자원보전과) ;
  • 조승주 (강원대학교, 산림환경시스템학과) ;
  • 성정원 (한국농수산대학교, 조경학과) ;
  • 윤정원 (국립수목원, DMZ산림생물자원보전과)
  • Received : 2023.09.06
  • Accepted : 2023.12.11
  • Published : 2024.02.01
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Abstract

This study was conducted to determine the conditions for breaking dormancy in the seeds of the North Korean plant resource, Trollius macropetalus, and to investigate its germination characteristics. The morphological characteristics and viability of T. macropetalus seeds were examined. Germination characteristics were investigated based on temperature conditions (15/6℃ & 25/15℃), cold stratification treatment, and GA3 treatment. The results showed that cold stratification treatment had no effect on breaking the dormancy of T. macropetalus. GA3 treatment significantly increased the germination rate of T. macropetalus and also improved the mean germination days and germination speed. However, compared to the 15/6℃ condition, the effectiveness of GA3 treatment for T. macropetalus decreased under the 25/15℃ condition. It was determined that T. macropetalus have a nondeep-type morpho-physiological dormancy. The most effective conditions for breaking dormancy were found to be the 15/6℃ temperature condition and GA3 treatment at 500 mg·L-1. The findings of this study are expected to provide valuable information for the mass propagation of T. macropetalus, which has potential applications in landscaping and pharmaceutical materials.

본 연구는 북한 식물자원 큰금매화의 종자 번식을 위해 발아에 대한 기초자료를 제시하고자 수행되었다. 큰금매화 종자의 형태적 특성과 활력을 검정하였고, 변온 조건(15/6℃ & 25/15℃), 저온층적처리 및 GA3 처리에 따른 발아 특성을 조사하였다. 연구 결과, 저온층적처리는 큰금매화 종자의 휴면타파에 효과가 없었다. GA3 처리는 큰금매화 종자의 발아율을 현저히 증가시켰고, 평균발아일수와 발아세 또한 유의미하게 향상시켰다. 한편, 15/6℃ 조건에 비해 25/15℃ 조건에서는 큰금매화 종자에 대한 GA3 처리의 효과가 감소하였다. 큰금매화 종자는 Nondeep-type의 형태·생리적휴면(MPD)을 가지는 것으로 판단되었고, 본 연구에서 가장 효율적인 발아 조건은 15/6℃ 변온조건과 GA3 500 mg·L-1 처리로 확인되었다. 이러한 본 연구의 결과는 고산성 북방계 식물인 큰금매화의 대량증식에 유용한 자료가 될 것이다.

Keywords

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