The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Development of a Selective Medium for Surveillance of Fusarium Head Blight Disease

  • Hosung Jeon (Department of Agricultural Biotechnology, Seoul National University) ;
  • Jung Wook Yang (Crop Cultivation and Environment Research Division, National Institute of Crop Science, Rural Development Administration) ;
  • Donghwan Shin (Department of Agricultural Biotechnology, Seoul National University) ;
  • Donggyu Min (Department of Agricultural Biotechnology, Seoul National University) ;
  • Byung Joo Kim (Crop Cultivation and Environment Research Division, National Institute of Crop Science, Rural Development Administration) ;
  • Kyunghun Min (Department of Agricultural Biotechnology, Seoul National University) ;
  • Hokyoung Son (Department of Agricultural Biotechnology, Seoul National University)
  • Received : 2023.12.17
  • Accepted : 2024.01.24
  • Published : 2024.04.01
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Abstract

Fusarium head blight (FHB), predominantly caused by Fusarium graminearum and F. asiaticum, is a significant fungal disease impacting small-grain cereals. The absence of highly resistant cultivars underscores the need for vigilant FHB surveillance to mitigate its detrimental effects. In 2023, a notable FHB outbreak occurred in the southern region of Korea. We assessed FHB disease severity by quantifying infected spikelets and grains. Isolating fungal pathogens from infected samples often encounters interference from various microorganisms. We developed a cost-effective, selective medium, named BGT (Burkholderia glumae Toxoflavin) medium, utilizing B. glumae, which is primarily known for causing bacterial panicle blight in rice. This medium exhibited selective growth properties, predominantly supporting Fusarium spp., while substantially inhibiting the growth of other fungi. Using the BGT medium, we isolated F. graminearum and F. asiaticum from infected wheat and barley samples across Korea. To further streamline the process, we used a direct PCR approach to amplify the translation elongation factor 1-α (TEF-1α) region without a separate genomic DNA extraction step. Phylogenetic analysis of the TEF-1α region revealed that the majority of the isolates were identified as F. asiaticum. Our results demonstrate that BGT medium is an effective tool for FHB diagnosis and Fusarium strain isolation.

Keywords

Acknowledgement

This work was supported by the National Research Foundation of Korea (2022R1I1A1A01065138 and 2021R1C1C1004200) and the project PJ015741022024 of National Institute of Crop Science, Rural Development Administration, Republic of Korea. We thank Eunhye Goo for providing the B. glumae BGR1 strain.

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